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The map label for this gene is xthA [H]

Identifier: 225853439

GI number: 225853439

Start: 1945122

End: 1945928

Strand: Direct

Name: xthA [H]

Synonym: BMEA_A2062

Alternate gene names: 225853439

Gene position: 1945122-1945928 (Clockwise)

Preceding gene: 225853438

Following gene: 225853442

Centisome position: 91.5

GC content: 58.36

Gene sequence:

>807_bases
ATGGCCTTTTCCATTGCTACCTGGAACATCAATTCGGTGCGTCTGCGCATGCCGCTTGTCGAGCAGTTTCTGCGCGATTA
TCAGCCCGATGTTCTGTGTTTGCAGGAAACCAAGTGCCCCGACGACCTGTTTCCCGCCAAGGGTTTTCGTGCGCTCGGCT
ATGAGCATATCGCGATCAGCGGCCAGAAAGGTTATCACGGGGTCGCCACGGTTTCACGCCGCCCGCTGGACGGTGTGGAG
AAGGTAGACTTCTGCAATATGGGCGATTGCCGCCACCTGAGCGCCGTCGTTGAGGCGGGCGACAGGAAACTGCGCATCCA
TAATTTCTATGTACCGGCGGGCGGCGACGAGCCGGACCCGGAAATCAACCCGAAATTTGCGCACAAGCTGGCATTTCTTG
AGGAAATGCGCGCCATTATTGCCGACCGGAAGGACGGATGTTCATCGCTTCTCGTCGGCGATCTCAACATCGCGCCGCTC
GAAAATGACGTCTGGTCGCACAAGCAGCTTTTGAAGATCGTCAGCCATACGCCGATCGAGACCGAAACGCTGGAAGACCT
GCGGATCAAGGGCGGCTGGAGCGACCTCATGCGCCATCTCATTGCGGCGGACCAGAAAATCTATACCTGGTGGAGCTATC
GCGCCAAGGATTGGGATGCCGCCGATCGCGGCCGCAGGCTGGACCATGTATGGGGTTCGGCTGATCTGGAAAGCCATAAC
AAGGGCTTGCAGGTGCTGCGCGATGCGCGCGGCTGGGACCGCCCTTCGGATCATGTGCCGGTGATTGCGACTTTCGATCT
GGATTGA

Upstream 100 bases:

>100_bases
ATATCCCCGTAATCATACTCTTGTGACATGAGTATGTTTGCAATTATCGGATAAATGGTTAGTTTCGCGACCAACCTGTC
AAAAGTCGCGAGTTCTTTCC

Downstream 100 bases:

>100_bases
GAAGATCAGTCGCGGTTTGCAAGCCGCGGCGCGACCTGCTCGATCTGGCCGATAAGCTCGACAAGATTGCGGCTGATATG
CGCATGAAGCGCGGCGGCCA

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III [H]

Number of amino acids: Translated: 268; Mature: 267

Protein sequence:

>268_residues
MAFSIATWNINSVRLRMPLVEQFLRDYQPDVLCLQETKCPDDLFPAKGFRALGYEHIAISGQKGYHGVATVSRRPLDGVE
KVDFCNMGDCRHLSAVVEAGDRKLRIHNFYVPAGGDEPDPEINPKFAHKLAFLEEMRAIIADRKDGCSSLLVGDLNIAPL
ENDVWSHKQLLKIVSHTPIETETLEDLRIKGGWSDLMRHLIAADQKIYTWWSYRAKDWDAADRGRRLDHVWGSADLESHN
KGLQVLRDARGWDRPSDHVPVIATFDLD

Sequences:

>Translated_268_residues
MAFSIATWNINSVRLRMPLVEQFLRDYQPDVLCLQETKCPDDLFPAKGFRALGYEHIAISGQKGYHGVATVSRRPLDGVE
KVDFCNMGDCRHLSAVVEAGDRKLRIHNFYVPAGGDEPDPEINPKFAHKLAFLEEMRAIIADRKDGCSSLLVGDLNIAPL
ENDVWSHKQLLKIVSHTPIETETLEDLRIKGGWSDLMRHLIAADQKIYTWWSYRAKDWDAADRGRRLDHVWGSADLESHN
KGLQVLRDARGWDRPSDHVPVIATFDLD
>Mature_267_residues
AFSIATWNINSVRLRMPLVEQFLRDYQPDVLCLQETKCPDDLFPAKGFRALGYEHIAISGQKGYHGVATVSRRPLDGVEK
VDFCNMGDCRHLSAVVEAGDRKLRIHNFYVPAGGDEPDPEINPKFAHKLAFLEEMRAIIADRKDGCSSLLVGDLNIAPLE
NDVWSHKQLLKIVSHTPIETETLEDLRIKGGWSDLMRHLIAADQKIYTWWSYRAKDWDAADRGRRLDHVWGSADLESHNK
GLQVLRDARGWDRPSDHVPVIATFDLD

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction [H]

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Escherichia coli, GI1788046, Length=272, Percent_Identity=29.7794117647059, Blast_Score=101, Evalue=4e-23,
Organism=Drosophila melanogaster, GI221330655, Length=276, Percent_Identity=26.8115942028986, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI17136678, Length=276, Percent_Identity=26.8115942028986, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 30454; Mature: 30323

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFSIATWNINSVRLRMPLVEQFLRDYQPDVLCLQETKCPDDLFPAKGFRALGYEHIAIS
CEEEEEEECCCCEEEECHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHCCEEEEEEE
GQKGYHGVATVSRRPLDGVEKVDFCNMGDCRHLSAVVEAGDRKLRIHNFYVPAGGDEPDP
CCCCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEEEECCCCCCCCC
EINPKFAHKLAFLEEMRAIIADRKDGCSSLLVGDLNIAPLENDVWSHKQLLKIVSHTPIE
CCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCC
TETLEDLRIKGGWSDLMRHLIAADQKIYTWWSYRAKDWDAADRGRRLDHVWGSADLESHN
HHHHHHHEECCCHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHCCCHHHHCCCCCCHHCH
KGLQVLRDARGWDRPSDHVPVIATFDLD
HHHHHHHHHCCCCCCCCCCCEEEEEECC
>Mature Secondary Structure 
AFSIATWNINSVRLRMPLVEQFLRDYQPDVLCLQETKCPDDLFPAKGFRALGYEHIAIS
EEEEEEECCCCEEEECHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHCCEEEEEEE
GQKGYHGVATVSRRPLDGVEKVDFCNMGDCRHLSAVVEAGDRKLRIHNFYVPAGGDEPDP
CCCCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEEEECCCCCCCCC
EINPKFAHKLAFLEEMRAIIADRKDGCSSLLVGDLNIAPLENDVWSHKQLLKIVSHTPIE
CCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCC
TETLEDLRIKGGWSDLMRHLIAADQKIYTWWSYRAKDWDAADRGRRLDHVWGSADLESHN
HHHHHHHEECCCHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHCCCHHHHCCCCCCHHCH
KGLQVLRDARGWDRPSDHVPVIATFDLD
HHHHHHHHHCCCCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]