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The map label for this gene is aspA [H]

Identifier: 225853394

GI number: 225853394

Start: 1905818

End: 1907269

Strand: Direct

Name: aspA [H]

Synonym: BMEA_A2015

Alternate gene names: 225853394

Gene position: 1905818-1907269 (Clockwise)

Preceding gene: 225853393

Following gene: 225853395

Centisome position: 89.66

GC content: 58.54

Gene sequence:

>1452_bases
ATGACAACCCTGGAAAATATTCATCCCCTGACACGGCGCGAGCAGGATTCCCTTGGGGAGCGCGATATTCCCATGGATGC
CTATTTCGGCATCCAGACCCTACGGGCAGTTGAAAACTTCTCGCTTTCCGATGTTGCGCTGAACCATATTCCTGCCCTCG
TTCGCGCCCTTGCAATGGTCAAGAAAGCGGCTGCCACCGCCAATTACAAGCTGCGGCAGCTGCCGGAGCCCAAATATGCG
GCCATCGTCGCCGCCTGCGACGACATCATTGACGGGCTTTTGATGGAGCAATTCGTCGTGGATGTGTTTCAGGGCGGCGC
GGGCACCTCAAGCAATATGAACGCAAATGAGGTTATCGCAAACCGCGCCCTTGAACATCTGGGGCGGCCGCGAGGCGACT
ACCAGACGATCCATCCCAATGATGACGTCAACATGTCGCAGTCCACCAATGATGTGTATCCAACCGCGGTAAGGCTGGCG
CTTCTTCTCAGCCAGAACCAGGTTCAGACGGCGCTGCACCGGCTGATTGCCGCCTTTGAAGCAAAGGGGCGGGAATTCGC
GACGGTCATCAAGATCGGGCGCACACAGTTGCAGGATGCGGTTCCGATCACTCTTGGACAGGAGTTCGAGGCATTTGCCG
CAACGTTGCGGGAGGACACAGCCCGGCTGGAGGAGGTTGCCGCGCTTTTCCGCGAAGTAAACCTCGGTGGAACCGCTATC
GGCACGCGCATCAACGCCTCGCACGCCTATGCCGAACAGGCCATCGCGGAACTTTCGCAGATTTCCGGTATCGAACTGAA
AGCCGCCGGCAATCTCGAGGAAGCGAGCTGGGATACCGGTGCGTTCGTCACCTTTTCCGGGATATTGCGCCGGATCGCAG
TCAAGCTTTCCAAGATCGCCAACGATCTGCGGCTACTCTCAAGCGGGCCGCGAAGCGGCCTTGGTGAAATCCGCCTGCCT
GCGGTTCAGCCGGGATCGTCGATCATGCCGGGAAAGGTCAATCCGGTCATACCGGAATCGGTCAATCAGGTCTGCTATCA
GGTGATCGGCAATGACCTAACTGTAACCATGGCAGCCGAAAGCGGGCAATTGCAGCTCAACGCGTTCGAGCCGCTGATCG
TCTACAATATCCTCAGTTCCATGCGACTGCTCGGCCGCGCCATGACCAATCTGGCCGAACGTTGCGTCGACGGAATTGAA
GCCAATGTGGAACGCTGTCGCGCAGGCGCGGAGGAAAGCATTTCGCTTGCAACCGCCCTCGTCCCGGTCGTGGGCTATGC
CAGGGCGGCAGAGATCGCCAAGCAGGCGCTTGCCTCAGGGCAGACCGTCATGGAAGTGGCGATTTCCAAGGGGCTGGACG
CATCTGCGCTCACGATAATGCTTGACCCCTTGAGAATGGCATTCCCGCCGGAAACGCACGACAAGGAGGCACATCATGCA
TCTGTCGGCTAA

Upstream 100 bases:

>100_bases
GTGCTGCCGTGCGCGCCGACTATTTCGGCACGAAAGCAGAGCTTGAAACGGCCGCTGGCAAAACCATGAAACCGGCCCAC
GCCGCAGCCTGAGAGCCATC

Downstream 100 bases:

>100_bases
GAAGACCTCCAGTGCCGTCGCCATGCGCGCGGTCAGCAAATGGTACGGGCAATTTCAGGTTCTCAAGGACATTGATTTCG
ATGTCGCCAGAGGCGAACGG

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 483; Mature: 482

Protein sequence:

>483_residues
MTTLENIHPLTRREQDSLGERDIPMDAYFGIQTLRAVENFSLSDVALNHIPALVRALAMVKKAAATANYKLRQLPEPKYA
AIVAACDDIIDGLLMEQFVVDVFQGGAGTSSNMNANEVIANRALEHLGRPRGDYQTIHPNDDVNMSQSTNDVYPTAVRLA
LLLSQNQVQTALHRLIAAFEAKGREFATVIKIGRTQLQDAVPITLGQEFEAFAATLREDTARLEEVAALFREVNLGGTAI
GTRINASHAYAEQAIAELSQISGIELKAAGNLEEASWDTGAFVTFSGILRRIAVKLSKIANDLRLLSSGPRSGLGEIRLP
AVQPGSSIMPGKVNPVIPESVNQVCYQVIGNDLTVTMAAESGQLQLNAFEPLIVYNILSSMRLLGRAMTNLAERCVDGIE
ANVERCRAGAEESISLATALVPVVGYARAAEIAKQALASGQTVMEVAISKGLDASALTIMLDPLRMAFPPETHDKEAHHA
SVG

Sequences:

>Translated_483_residues
MTTLENIHPLTRREQDSLGERDIPMDAYFGIQTLRAVENFSLSDVALNHIPALVRALAMVKKAAATANYKLRQLPEPKYA
AIVAACDDIIDGLLMEQFVVDVFQGGAGTSSNMNANEVIANRALEHLGRPRGDYQTIHPNDDVNMSQSTNDVYPTAVRLA
LLLSQNQVQTALHRLIAAFEAKGREFATVIKIGRTQLQDAVPITLGQEFEAFAATLREDTARLEEVAALFREVNLGGTAI
GTRINASHAYAEQAIAELSQISGIELKAAGNLEEASWDTGAFVTFSGILRRIAVKLSKIANDLRLLSSGPRSGLGEIRLP
AVQPGSSIMPGKVNPVIPESVNQVCYQVIGNDLTVTMAAESGQLQLNAFEPLIVYNILSSMRLLGRAMTNLAERCVDGIE
ANVERCRAGAEESISLATALVPVVGYARAAEIAKQALASGQTVMEVAISKGLDASALTIMLDPLRMAFPPETHDKEAHHA
SVG
>Mature_482_residues
TTLENIHPLTRREQDSLGERDIPMDAYFGIQTLRAVENFSLSDVALNHIPALVRALAMVKKAAATANYKLRQLPEPKYAA
IVAACDDIIDGLLMEQFVVDVFQGGAGTSSNMNANEVIANRALEHLGRPRGDYQTIHPNDDVNMSQSTNDVYPTAVRLAL
LLSQNQVQTALHRLIAAFEAKGREFATVIKIGRTQLQDAVPITLGQEFEAFAATLREDTARLEEVAALFREVNLGGTAIG
TRINASHAYAEQAIAELSQISGIELKAAGNLEEASWDTGAFVTFSGILRRIAVKLSKIANDLRLLSSGPRSGLGEIRLPA
VQPGSSIMPGKVNPVIPESVNQVCYQVIGNDLTVTMAAESGQLQLNAFEPLIVYNILSSMRLLGRAMTNLAERCVDGIEA
NVERCRAGAEESISLATALVPVVGYARAAEIAKQALASGQTVMEVAISKGLDASALTIMLDPLRMAFPPETHDKEAHHAS
VG

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=463, Percent_Identity=43.6285097192225, Blast_Score=357, Evalue=2e-98,
Organism=Escherichia coli, GI87082375, Length=442, Percent_Identity=51.8099547511312, Blast_Score=445, Evalue=1e-126,
Organism=Escherichia coli, GI1787896, Length=430, Percent_Identity=45.1162790697674, Blast_Score=358, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI17553882, Length=442, Percent_Identity=43.6651583710407, Blast_Score=360, Evalue=1e-99,
Organism=Caenorhabditis elegans, GI32565146, Length=309, Percent_Identity=44.336569579288, Blast_Score=256, Evalue=2e-68,
Organism=Saccharomyces cerevisiae, GI6324993, Length=444, Percent_Identity=42.7927927927928, Blast_Score=336, Evalue=5e-93,
Organism=Drosophila melanogaster, GI78710009, Length=461, Percent_Identity=41.648590021692, Blast_Score=359, Evalue=2e-99,
Organism=Drosophila melanogaster, GI24640177, Length=442, Percent_Identity=44.3438914027149, Blast_Score=357, Evalue=1e-98,
Organism=Drosophila melanogaster, GI24640179, Length=442, Percent_Identity=44.3438914027149, Blast_Score=357, Evalue=1e-98,
Organism=Drosophila melanogaster, GI24662684, Length=463, Percent_Identity=41.036717062635, Blast_Score=354, Evalue=9e-98,
Organism=Drosophila melanogaster, GI24583245, Length=466, Percent_Identity=40.343347639485, Blast_Score=347, Evalue=7e-96,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 51931; Mature: 51800

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLENIHPLTRREQDSLGERDIPMDAYFGIQTLRAVENFSLSDVALNHIPALVRALAMV
CCCCHHCCCHHCCHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
KKAAATANYKLRQLPEPKYAAIVAACDDIIDGLLMEQFVVDVFQGGAGTSSNMNANEVIA
HHHHHHCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
NRALEHLGRPRGDYQTIHPNDDVNMSQSTNDVYPTAVRLALLLSQNQVQTALHRLIAAFE
HHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKGREFATVIKIGRTQLQDAVPITLGQEFEAFAATLREDTARLEEVAALFREVNLGGTAI
HCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
GTRINASHAYAEQAIAELSQISGIELKAAGNLEEASWDTGAFVTFSGILRRIAVKLSKIA
CCEECCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
NDLRLLSSGPRSGLGEIRLPAVQPGSSIMPGKVNPVIPESVNQVCYQVIGNDLTVTMAAE
HHHHHHHCCCCCCCCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECC
SGQLQLNAFEPLIVYNILSSMRLLGRAMTNLAERCVDGIEANVERCRAGAEESISLATAL
CCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
VPVVGYARAAEIAKQALASGQTVMEVAISKGLDASALTIMLDPLRMAFPPETHDKEAHHA
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHEEECHHHHCCCCCCCCCCCCCC
SVG
CCC
>Mature Secondary Structure 
TTLENIHPLTRREQDSLGERDIPMDAYFGIQTLRAVENFSLSDVALNHIPALVRALAMV
CCCHHCCCHHCCHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
KKAAATANYKLRQLPEPKYAAIVAACDDIIDGLLMEQFVVDVFQGGAGTSSNMNANEVIA
HHHHHHCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
NRALEHLGRPRGDYQTIHPNDDVNMSQSTNDVYPTAVRLALLLSQNQVQTALHRLIAAFE
HHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKGREFATVIKIGRTQLQDAVPITLGQEFEAFAATLREDTARLEEVAALFREVNLGGTAI
HCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
GTRINASHAYAEQAIAELSQISGIELKAAGNLEEASWDTGAFVTFSGILRRIAVKLSKIA
CCEECCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
NDLRLLSSGPRSGLGEIRLPAVQPGSSIMPGKVNPVIPESVNQVCYQVIGNDLTVTMAAE
HHHHHHHCCCCCCCCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECC
SGQLQLNAFEPLIVYNILSSMRLLGRAMTNLAERCVDGIEANVERCRAGAEESISLATAL
CCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
VPVVGYARAAEIAKQALASGQTVMEVAISKGLDASALTIMLDPLRMAFPPETHDKEAHHA
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHEEECHHHHCCCCCCCCCCCCCC
SVG
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3096982 [H]