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The map label for this gene is gloB

Identifier: 225853374

GI number: 225853374

Start: 1886500

End: 1887282

Strand: Direct

Name: gloB

Synonym: BMEA_A1993

Alternate gene names: 225853374

Gene position: 1886500-1887282 (Clockwise)

Preceding gene: 225853373

Following gene: 225853375

Centisome position: 88.75

GC content: 58.24

Gene sequence:

>783_bases
ATGCACAGGATGGAACAAAGGCTTGAAATCGAACAGTTCATCTGCCGCAGCGATAATTACGGCGTTCTGATCCATGATCC
CGAAAGCGCGCTGACAGCCACCATCGATGCGCCCGACGCCTATGCCATCGAAGCGGCACTGGAACGGCGCGGCTGGACGC
TCGATTTCATCTTCACCACCCATCACCATCTCGATCATGTCGAAGGCAATGAACCGCTGAAAGAAAAGTTCGGCGTCAGC
ATTATCGGGCCGGAGGCCGAAAAGGCAAAAATCCCCGGCATCGACCGCACGGTGAAGGGCGGCGACGAATTTACCTTCGG
GCTTTTCAAGGTGAAGGTCATTTCCACGCCCGGCCATACGGCGGGTGGGATTTCCTATTACCTGCCGGATGCGAAAGTCG
TCTTCACCGGCGACACGCTTTTCGCACTTGGCTGCGGGCGGCTTTTCGAGGGCACGCCCGCCACCATGTTCCATTCACTC
GAAAAGCTGGTGGCGCTGCCGGGCGATACCGCGCTTTATTGCGGCCATGAATATACGCAAAACAATGCCCGCTTCGCACT
GACCATCGACCCTGACAATTCGGCGCTGAAAGAACGGGCGAAGGAAATTGCCCGCCTGCGCGCGCACGAGCGGATGACGC
TGCCATCCACCATCGCGCTCGAAATGGCCACCAATCCATTCCTGCGCTGGCATGACCGCACCATTCGCGCGCGCCTCGGC
CTGCAGGATGCGCCAGACGAAGCCGTGTTTGCGGAAATCCGCAAGCGAAAGGATATGTTTTGA

Upstream 100 bases:

>100_bases
CCATTGTCTTCACGCTTTTACAAATGCCGCTTATCCAGAAGCATTCGTTGACTGATGAGGAAAACACCGCATCATAACAG
CCTGAAGAGGGAGGAGCGCC

Downstream 100 bases:

>100_bases
ACACACTTCCCACGCCTTATGAAGCTTTGATTTCAAGGACCAGGAGATGACGGCAATATCCCGTTTCAAGATCACAAGCC
GCGCTGTGTTGCTCGGCCTT

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MHRMEQRLEIEQFICRSDNYGVLIHDPESALTATIDAPDAYAIEAALERRGWTLDFIFTTHHHLDHVEGNEPLKEKFGVS
IIGPEAEKAKIPGIDRTVKGGDEFTFGLFKVKVISTPGHTAGGISYYLPDAKVVFTGDTLFALGCGRLFEGTPATMFHSL
EKLVALPGDTALYCGHEYTQNNARFALTIDPDNSALKERAKEIARLRAHERMTLPSTIALEMATNPFLRWHDRTIRARLG
LQDAPDEAVFAEIRKRKDMF

Sequences:

>Translated_260_residues
MHRMEQRLEIEQFICRSDNYGVLIHDPESALTATIDAPDAYAIEAALERRGWTLDFIFTTHHHLDHVEGNEPLKEKFGVS
IIGPEAEKAKIPGIDRTVKGGDEFTFGLFKVKVISTPGHTAGGISYYLPDAKVVFTGDTLFALGCGRLFEGTPATMFHSL
EKLVALPGDTALYCGHEYTQNNARFALTIDPDNSALKERAKEIARLRAHERMTLPSTIALEMATNPFLRWHDRTIRARLG
LQDAPDEAVFAEIRKRKDMF
>Mature_260_residues
MHRMEQRLEIEQFICRSDNYGVLIHDPESALTATIDAPDAYAIEAALERRGWTLDFIFTTHHHLDHVEGNEPLKEKFGVS
IIGPEAEKAKIPGIDRTVKGGDEFTFGLFKVKVISTPGHTAGGISYYLPDAKVVFTGDTLFALGCGRLFEGTPATMFHSL
EKLVALPGDTALYCGHEYTQNNARFALTIDPDNSALKERAKEIARLRAHERMTLPSTIALEMATNPFLRWHDRTIRARLG
LQDAPDEAVFAEIRKRKDMF

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family

Homologues:

Organism=Homo sapiens, GI94538322, Length=263, Percent_Identity=35.7414448669202, Blast_Score=154, Evalue=8e-38,
Organism=Homo sapiens, GI94538320, Length=260, Percent_Identity=35.7692307692308, Blast_Score=154, Evalue=9e-38,
Organism=Homo sapiens, GI21703352, Length=256, Percent_Identity=34.765625, Blast_Score=153, Evalue=1e-37,
Organism=Homo sapiens, GI116642887, Length=256, Percent_Identity=34.765625, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI14150041, Length=245, Percent_Identity=32.6530612244898, Blast_Score=138, Evalue=4e-33,
Organism=Homo sapiens, GI46361987, Length=188, Percent_Identity=34.5744680851064, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI41327741, Length=219, Percent_Identity=28.7671232876712, Blast_Score=84, Evalue=1e-16,
Organism=Escherichia coli, GI1786406, Length=249, Percent_Identity=40.9638554216867, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1787158, Length=176, Percent_Identity=30.6818181818182, Blast_Score=72, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17536925, Length=262, Percent_Identity=35.4961832061069, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17538952, Length=137, Percent_Identity=35.036496350365, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324614, Length=263, Percent_Identity=32.319391634981, Blast_Score=108, Evalue=9e-25,
Organism=Saccharomyces cerevisiae, GI6320478, Length=243, Percent_Identity=32.5102880658436, Blast_Score=105, Evalue=9e-24,
Organism=Drosophila melanogaster, GI21356335, Length=262, Percent_Identity=38.5496183206107, Blast_Score=173, Evalue=1e-43,
Organism=Drosophila melanogaster, GI24667711, Length=260, Percent_Identity=38.8461538461538, Blast_Score=172, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24667703, Length=262, Percent_Identity=38.5496183206107, Blast_Score=172, Evalue=2e-43,
Organism=Drosophila melanogaster, GI221330176, Length=218, Percent_Identity=28.4403669724771, Blast_Score=89, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLO2_BRUA1 (B2S889)

Other databases:

- EMBL:   CP000887
- RefSeq:   YP_001935772.1
- ProteinModelPortal:   B2S889
- SMR:   B2S889
- GeneID:   6326916
- GenomeReviews:   CP000887_GR
- KEGG:   bmc:BAbS19_I18160
- HOGENOM:   HBG753931
- OMA:   WCAHEYT
- ProtClustDB:   CLSK898025
- HAMAP:   MF_01374
- InterPro:   IPR001279
- InterPro:   IPR017782
- SMART:   SM00849
- TIGRFAMs:   TIGR03413

Pfam domain/function: PF00753 Lactamase_B

EC number: =3.1.2.6

Molecular weight: Translated: 29141; Mature: 29141

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRMEQRLEIEQFICRSDNYGVLIHDPESALTATIDAPDAYAIEAALERRGWTLDFIFTT
CCHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEECCCCCHHHHHHHHHCCCEEEEEEEC
HHHLDHVEGNEPLKEKFGVSIIGPEAEKAKIPGIDRTVKGGDEFTFGLFKVKVISTPGHT
CCCCCCCCCCCCHHHHCCEEEECCCCCCCCCCCCCCEECCCCCEEEEEEEEEEEECCCCC
AGGISYYLPDAKVVFTGDTLFALGCGRLFEGTPATMFHSLEKLVALPGDTALYCGHEYTQ
CCCEEEECCCCEEEEECCEEEEEECCHHCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCC
NNARFALTIDPDNSALKERAKEIARLRAHERMTLPSTIALEMATNPFLRWHDRTIRARLG
CCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEECCCEEEEECC
LQDAPDEAVFAEIRKRKDMF
CCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MHRMEQRLEIEQFICRSDNYGVLIHDPESALTATIDAPDAYAIEAALERRGWTLDFIFTT
CCHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEECCCCCHHHHHHHHHCCCEEEEEEEC
HHHLDHVEGNEPLKEKFGVSIIGPEAEKAKIPGIDRTVKGGDEFTFGLFKVKVISTPGHT
CCCCCCCCCCCCHHHHCCEEEECCCCCCCCCCCCCCEECCCCCEEEEEEEEEEEECCCCC
AGGISYYLPDAKVVFTGDTLFALGCGRLFEGTPATMFHSLEKLVALPGDTALYCGHEYTQ
CCCEEEECCCCEEEEECCEEEEEECCHHCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCC
NNARFALTIDPDNSALKERAKEIARLRAHERMTLPSTIALEMATNPFLRWHDRTIRARLG
CCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEECCCEEEEECC
LQDAPDEAVFAEIRKRKDMF
CCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA