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The map label for this gene is lpdA [H]

Identifier: 225853356

GI number: 225853356

Start: 1868546

End: 1869949

Strand: Reverse

Name: lpdA [H]

Synonym: BMEA_A1975

Alternate gene names: 225853356

Gene position: 1869949-1868546 (Counterclockwise)

Preceding gene: 225853357

Following gene: 225853353

Centisome position: 87.97

GC content: 58.4

Gene sequence:

>1404_bases
ATGTCTTACGATGTGGTTGTCATCGGCACGGGCCCCGGCGGTTATGTGGCCGCGATCAAGGCTGCCCAGCTTGGCCTTAA
GGTTGCGGTGGTGGAAAAGCGCAAGACCTTCGGTGGCACCTGCCTGAATATTGGCTGCATTCCTTCCAAGGCGCTTCTTC
ATGCGTCGGAAGTTTTTGCCGAAGCCGGTCACTCGTTCGATACGCTTGGCGTGGAAGTGACGCCGAAGCTCAATCTCACG
AAGATGCTGGCCCACAAGGACACGACCGTGAAGGCCAATGTCTCGGGCGTTGAATTCCTGTTCAAGAAGAACAAGATTAC
CCCTTACATCGGCACCGGCAAGATCGTTGGCAAGGGCAAGGTTTCCGTGACCTCGGAAGATGGCAAGGTTGAGGAAATCG
AAGCAAAGAACATCATCATCGCCACCGGTTCGGATGTCGCGGGCATTCCGGGCGTCAAGGTGGATATTGACGAGAAGGTC
ATCGTTTCCTCGACCGGTGCGCTCTCCTTCGACAAGGTGCCGGGCAGCCTTATCGTTGTTGGCGGGGGCGTGATCGGCCT
GGAGCTTGGTTCCGTCTGGGCGCGCCTTGGCGCGAAAGTTACGGTCGTGGAATATCTCGACAAGGTGCTTGGCCCGATGG
ACGGCGAAGTGTCGAAGCAGTTCCAGCGCCTGCTTGAAAAGCAGGGCATTGCCTTCAAGCTTGGCGCCAAGGTTACGGGC
GTCGAGAAAGTCGGAAAGGGCGCAAAGATAACGTTTGAACCGGTCAAGGGTGGCGATGCCGAAACGCTTGAAGCCGATGC
GGTTCTGATCGCGACCGGCCGTCGTCCTTACACGGATGGCCTCGGCCTGCAGGAAGCCGGTGTTGCTGTCGATGAGCGCG
GCCGCGTTGCGATCGACGATCATTGGCGCACCAATGTCGAAGGCATTTACGCCATTGGCGACGTGGTGCAGGGGCCAATG
CTGGCACACAAGGCCGAAGATGAAGGCATTGCGGTTGCCGAAATCATTGCCGGTCAGGCCGGGCATGTGAATTTCGACGT
CATTCCGAGCGTCGTTTATACCCAGCCGGAAGTGGCTTCCGTCGGCAAGACCGAAGAAGAGCTGAAGGCCGCCGGTATCG
ACTATAAGGTCGGCAAGTTCCCGTTCACGGCCAATGGCCGCGCGCGCGCCATGCTGCATACGGACGGTTTCGTCAAAATC
CTCGCTGACAAGGCGACAGACCGTGTTCTGGGCGCGCATATCCTTGGCTACAATGCCGGTGAAATGATCCACGAACTGGC
CGTGCTGATGGAATTTGGCGGTTCGTCGGAAGATCTGGCCCGCACCTGCCACGCCCATCCGACCATGTCGGAAACCGTGC
GTGAATCGGCGCTGGCAACCTTCGCCAAGCCGATCCATATGTGA

Upstream 100 bases:

>100_bases
CTTCGTGGTGATCGAAACTGCTCACCTTGGAATGAAAGAACGTGCCCGGATGAACCGGGCGTTCTAAAACGTTCAGGATA
GACTGAAGAAGGATAGTTCA

Downstream 100 bases:

>100_bases
TCGGTATTATACATGCTTTCGACAAAGGCCCGCGCAAGCGGGCCTTTTTCATGCTGATTGGATTATGCTATAGAGCGCCG
ATCTGATTGAATCAGATCGG

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase 3; LPD-3 [H]

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MSYDVVVIGTGPGGYVAAIKAAQLGLKVAVVEKRKTFGGTCLNIGCIPSKALLHASEVFAEAGHSFDTLGVEVTPKLNLT
KMLAHKDTTVKANVSGVEFLFKKNKITPYIGTGKIVGKGKVSVTSEDGKVEEIEAKNIIIATGSDVAGIPGVKVDIDEKV
IVSSTGALSFDKVPGSLIVVGGGVIGLELGSVWARLGAKVTVVEYLDKVLGPMDGEVSKQFQRLLEKQGIAFKLGAKVTG
VEKVGKGAKITFEPVKGGDAETLEADAVLIATGRRPYTDGLGLQEAGVAVDERGRVAIDDHWRTNVEGIYAIGDVVQGPM
LAHKAEDEGIAVAEIIAGQAGHVNFDVIPSVVYTQPEVASVGKTEEELKAAGIDYKVGKFPFTANGRARAMLHTDGFVKI
LADKATDRVLGAHILGYNAGEMIHELAVLMEFGGSSEDLARTCHAHPTMSETVRESALATFAKPIHM

Sequences:

>Translated_467_residues
MSYDVVVIGTGPGGYVAAIKAAQLGLKVAVVEKRKTFGGTCLNIGCIPSKALLHASEVFAEAGHSFDTLGVEVTPKLNLT
KMLAHKDTTVKANVSGVEFLFKKNKITPYIGTGKIVGKGKVSVTSEDGKVEEIEAKNIIIATGSDVAGIPGVKVDIDEKV
IVSSTGALSFDKVPGSLIVVGGGVIGLELGSVWARLGAKVTVVEYLDKVLGPMDGEVSKQFQRLLEKQGIAFKLGAKVTG
VEKVGKGAKITFEPVKGGDAETLEADAVLIATGRRPYTDGLGLQEAGVAVDERGRVAIDDHWRTNVEGIYAIGDVVQGPM
LAHKAEDEGIAVAEIIAGQAGHVNFDVIPSVVYTQPEVASVGKTEEELKAAGIDYKVGKFPFTANGRARAMLHTDGFVKI
LADKATDRVLGAHILGYNAGEMIHELAVLMEFGGSSEDLARTCHAHPTMSETVRESALATFAKPIHM
>Mature_466_residues
SYDVVVIGTGPGGYVAAIKAAQLGLKVAVVEKRKTFGGTCLNIGCIPSKALLHASEVFAEAGHSFDTLGVEVTPKLNLTK
MLAHKDTTVKANVSGVEFLFKKNKITPYIGTGKIVGKGKVSVTSEDGKVEEIEAKNIIIATGSDVAGIPGVKVDIDEKVI
VSSTGALSFDKVPGSLIVVGGGVIGLELGSVWARLGAKVTVVEYLDKVLGPMDGEVSKQFQRLLEKQGIAFKLGAKVTGV
EKVGKGAKITFEPVKGGDAETLEADAVLIATGRRPYTDGLGLQEAGVAVDERGRVAIDDHWRTNVEGIYAIGDVVQGPML
AHKAEDEGIAVAEIIAGQAGHVNFDVIPSVVYTQPEVASVGKTEEELKAAGIDYKVGKFPFTANGRARAMLHTDGFVKIL
ADKATDRVLGAHILGYNAGEMIHELAVLMEFGGSSEDLARTCHAHPTMSETVRESALATFAKPIHM

Specific function: LPD-3 may substitute for lipoamide dehydrogenase of the 2-oxoglutarate dehydrogenase and pyruvate multienzyme complexes when the latter is inactive or missing [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=469, Percent_Identity=56.7164179104478, Blast_Score=518, Evalue=1e-147,
Organism=Homo sapiens, GI50301238, Length=469, Percent_Identity=30.0639658848614, Blast_Score=178, Evalue=1e-44,
Organism=Homo sapiens, GI33519430, Length=474, Percent_Identity=29.1139240506329, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI33519428, Length=474, Percent_Identity=29.1139240506329, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI33519426, Length=474, Percent_Identity=29.1139240506329, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI148277065, Length=474, Percent_Identity=29.1139240506329, Blast_Score=146, Evalue=5e-35,
Organism=Homo sapiens, GI148277071, Length=474, Percent_Identity=29.1139240506329, Blast_Score=145, Evalue=7e-35,
Organism=Homo sapiens, GI22035672, Length=463, Percent_Identity=30.0215982721382, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI291045266, Length=480, Percent_Identity=28.9583333333333, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI291045268, Length=472, Percent_Identity=28.1779661016949, Blast_Score=124, Evalue=2e-28,
Organism=Escherichia coli, GI1786307, Length=449, Percent_Identity=42.9844097995546, Blast_Score=325, Evalue=3e-90,
Organism=Escherichia coli, GI87082354, Length=460, Percent_Identity=31.7391304347826, Blast_Score=228, Evalue=8e-61,
Organism=Escherichia coli, GI87081717, Length=458, Percent_Identity=30.3493449781659, Blast_Score=194, Evalue=8e-51,
Organism=Escherichia coli, GI1789915, Length=440, Percent_Identity=30.2272727272727, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI1789065, Length=205, Percent_Identity=28.780487804878, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI32565766, Length=460, Percent_Identity=56.0869565217391, Blast_Score=510, Evalue=1e-145,
Organism=Caenorhabditis elegans, GI17557007, Length=472, Percent_Identity=30.2966101694915, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI71982272, Length=486, Percent_Identity=26.9547325102881, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI71983419, Length=449, Percent_Identity=26.9487750556793, Blast_Score=109, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI71983429, Length=454, Percent_Identity=27.5330396475771, Blast_Score=109, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17559934, Length=249, Percent_Identity=26.1044176706827, Blast_Score=75, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6321091, Length=475, Percent_Identity=53.0526315789474, Blast_Score=471, Evalue=1e-134,
Organism=Saccharomyces cerevisiae, GI6325240, Length=473, Percent_Identity=33.4038054968288, Blast_Score=246, Evalue=4e-66,
Organism=Saccharomyces cerevisiae, GI6325166, Length=465, Percent_Identity=27.9569892473118, Blast_Score=155, Evalue=1e-38,
Organism=Drosophila melanogaster, GI21358499, Length=469, Percent_Identity=54.7974413646055, Blast_Score=510, Evalue=1e-145,
Organism=Drosophila melanogaster, GI24640553, Length=483, Percent_Identity=30.2277432712215, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24640549, Length=483, Percent_Identity=30.2277432712215, Blast_Score=144, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24640551, Length=483, Percent_Identity=30.2277432712215, Blast_Score=144, Evalue=2e-34,
Organism=Drosophila melanogaster, GI17737741, Length=482, Percent_Identity=27.3858921161826, Blast_Score=119, Evalue=5e-27,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 49052; Mature: 48921

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYDVVVIGTGPGGYVAAIKAAQLGLKVAVVEKRKTFGGTCLNIGCIPSKALLHASEVFA
CCEEEEEEECCCCCEEEEEEHHHCCEEEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHH
EAGHSFDTLGVEVTPKLNLTKMLAHKDTTVKANVSGVEFLFKKNKITPYIGTGKIVGKGK
HCCCCEEEECEEECCCCCHHHHHHCCCCEEEECCCCEEEEEECCCCCEEECCCEEEECCE
VSVTSEDGKVEEIEAKNIIIATGSDVAGIPGVKVDIDEKVIVSSTGALSFDKVPGSLIVV
EEEECCCCCEEEEECCEEEEECCCCCCCCCCEEEECCCEEEEECCCCEEECCCCCEEEEE
GGGVIGLELGSVWARLGAKVTVVEYLDKVLGPMDGEVSKQFQRLLEKQGIAFKLGAKVTG
ECCEEEEEHHHHHHHHCCEEEHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEECCEEEC
VEKVGKGAKITFEPVKGGDAETLEADAVLIATGRRPYTDGLGLQEAGVAVDERGRVAIDD
HHHHCCCCEEEEEECCCCCCCEEECCEEEEEECCCCCCCCCCCHHCCCEECCCCCEEECC
HWRTNVEGIYAIGDVVQGPMLAHKAEDEGIAVAEIIAGQAGHVNFDVIPSVVYTQPEVAS
CCCCCCCEEEEEHHHHCCCHHEECCCCCCEEEEHHHCCCCCCEEHEECCCEEECCCCHHH
VGKTEEELKAAGIDYKVGKFPFTANGRARAMLHTDGFVKILADKATDRVLGAHILGYNAG
CCCCHHHHHHCCCCEECCCCEECCCCCEEEEEECCCEEEEEECHHHHHHHHHHEECCCHH
EMIHELAVLMEFGGSSEDLARTCHAHPTMSETVRESALATFAKPIHM
HHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SYDVVVIGTGPGGYVAAIKAAQLGLKVAVVEKRKTFGGTCLNIGCIPSKALLHASEVFA
CEEEEEEECCCCCEEEEEEHHHCCEEEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHH
EAGHSFDTLGVEVTPKLNLTKMLAHKDTTVKANVSGVEFLFKKNKITPYIGTGKIVGKGK
HCCCCEEEECEEECCCCCHHHHHHCCCCEEEECCCCEEEEEECCCCCEEECCCEEEECCE
VSVTSEDGKVEEIEAKNIIIATGSDVAGIPGVKVDIDEKVIVSSTGALSFDKVPGSLIVV
EEEECCCCCEEEEECCEEEEECCCCCCCCCCEEEECCCEEEEECCCCEEECCCCCEEEEE
GGGVIGLELGSVWARLGAKVTVVEYLDKVLGPMDGEVSKQFQRLLEKQGIAFKLGAKVTG
ECCEEEEEHHHHHHHHCCEEEHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEECCEEEC
VEKVGKGAKITFEPVKGGDAETLEADAVLIATGRRPYTDGLGLQEAGVAVDERGRVAIDD
HHHHCCCCEEEEEECCCCCCCEEECCEEEEEECCCCCCCCCCCHHCCCEECCCCCEEECC
HWRTNVEGIYAIGDVVQGPMLAHKAEDEGIAVAEIIAGQAGHVNFDVIPSVVYTQPEVAS
CCCCCCCEEEEEHHHHCCCHHEECCCCCCEEEEHHHCCCCCCEEHEECCCEEECCCCHHH
VGKTEEELKAAGIDYKVGKFPFTANGRARAMLHTDGFVKILADKATDRVLGAHILGYNAG
CCCCHHHHHHCCCCEECCCCEECCCCCEEEEEECCCEEEEEECHHHHHHHHHHEECCCHH
EMIHELAVLMEFGGSSEDLARTCHAHPTMSETVRESALATFAKPIHM
HHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]