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The map label for this gene is purH [H]

Identifier: 225853260

GI number: 225853260

Start: 1765546

End: 1767162

Strand: Direct

Name: purH [H]

Synonym: BMEA_A1866

Alternate gene names: 225853260

Gene position: 1765546-1767162 (Clockwise)

Preceding gene: 225853259

Following gene: 225853261

Centisome position: 83.06

GC content: 61.53

Gene sequence:

>1617_bases
ATGGCTGTCAGCTCCAAGCATATTCCCGCTCCCGATCTTCATCGGGTGCGCCGCGCCCTCCTTTCCGTTTCCGACAAAAC
CGGCCTGATCGATTTCGCCAAGGCGCTTCATGCCAATGGCGTCGAAATCCTCTCGACCGGCGGCACAGCAAAATCGATTG
CCGCCGCAGGCATTCCGGTGAAGGACGTATCCGAAATCACCGGCTTTCCGGAAATCATGGATGGGCGCGTCAAGACGCTG
CATCCGGCAGTCCATGGCGGCCTGCTCGCCGTGCGCAACGACCCGGAACATGTCGCGGCCATGGAAGAACACGGCATTGG
CGGCATCGATCTTGCCGTCATCAATCTTTATCCGTTCGAGGAAGTCCGCTTCAAGGGCGGCGACTACGACACGACCGTCG
AGAATATCGACATTGGCGGCCCGGCGATGATCCGCGCTTCGGCCAAGAACCACGCCTATGTCGCAACCGTCGTTGACCCT
GCCGATTATGCAGACGTCGTGGCTGAACTGGAAAAGCATTCCGGCTCCCTGCCGCTTGCCTTCCGCAAAAAGCTTGCCGC
CAAGGCCTTTTCGCGCACGGCAGCTTATGACGCGGCCATTTCCAACTGGTTTGCCGAAGCGATCGACGAAGAAACGCCGA
CCTATCGCGCGGTTGCCGGCAAGCTGCATTCCGTCATGCGCTACGGTGAAAACCCGCACCAGACGGCTGGCTTTTATCTC
ACCGGCGAAAAGCGCCCCGGCGTTGCCACTGCGACCCAGCTTCAGGGCAAGCAGCTTTCCTATAACAACATCAACGATAC
CGATGCGGCTTTTGAGCTGGTGGCCGAATTCGATCCCGCCCGCACCGCAGCCGTTGCCATCATCAAGCACGCCAATCCAT
GCGGCGTGGCGGAAGCGTCCACCATTAAGGAAGCCTACCTCAAGGCACTCGCCTGCGATCCGGTTTCGGCCTTTGGCGGC
ATTGTCGCGCTCAACAGGACGCTCGATGAGGAAGCCGCTGAAGAGATCGTGAAAACCTTCACAGAAGTCATCATCGCGCC
CGATGCCACCGAAGGCGCACAGGCCATCGTCGCGGCCAAGAAGAACCTGCGCCTGCTTGTCACCGGCGGCCTGCCGGACC
CGCGCGCCAAGGGCATTGCCGCCAAGACGGTTGCCGGTGGCCTGCTGGTCCAGTCGCGCGACAATGGTGTGGTGGACGAT
CTCGATCTCAAGGTCGTCACCAAGCGCGCACCGACCGAAGCCGAGCTTAACGATCTGAAATTCGCCTTCCGCGTCGGCAA
GCATGTGAAGTCGAACGCCATCGTCTATGTGAAGGACGGCGCAACGGTCGGCATTGGCGCAGGCCAGATGAGCCGCGTGG
ATTCAGCCCGCATTGCCGCCCGCAAGGCAGAAGATGCGGCCGAAGCCGCCGGCCTTGCAGCGCCGCTCACCAAGGGCTGC
GTGGTCGCGTCCGATGCATTCTTCCCCTTTGCAGACGGTCTGCTTTCCGCTGTCGAGGCCGGTGCAACCGCCGTCATCCA
GCCGGGCGGCTCCATGCGTGACGACGAAGTGATCGCCGCTGCCGATGAACATGGCATCGCCATGGTCATGACGGGGATGC
GCCACTTCCGCCACTGA

Upstream 100 bases:

>100_bases
AATCGGTCTTTTGCGGTAGGATTCTCGGCGCGCTTGTGTTAGGGCGAGCCGTCCACTTTCCTGAACGCATCCGTTTCAGC
AACGAAGAGACCAACTCCCT

Downstream 100 bases:

>100_bases
TCGGAACATATAAAGCAAAGGCCCCGGAACATATGTTTCGGGGCCTTTGTCATATCAGACGGTCCGCCGGATAGGGCGTA
ATCAGCGTCAGCGCAAAGCC

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 538; Mature: 537

Protein sequence:

>538_residues
MAVSSKHIPAPDLHRVRRALLSVSDKTGLIDFAKALHANGVEILSTGGTAKSIAAAGIPVKDVSEITGFPEIMDGRVKTL
HPAVHGGLLAVRNDPEHVAAMEEHGIGGIDLAVINLYPFEEVRFKGGDYDTTVENIDIGGPAMIRASAKNHAYVATVVDP
ADYADVVAELEKHSGSLPLAFRKKLAAKAFSRTAAYDAAISNWFAEAIDEETPTYRAVAGKLHSVMRYGENPHQTAGFYL
TGEKRPGVATATQLQGKQLSYNNINDTDAAFELVAEFDPARTAAVAIIKHANPCGVAEASTIKEAYLKALACDPVSAFGG
IVALNRTLDEEAAEEIVKTFTEVIIAPDATEGAQAIVAAKKNLRLLVTGGLPDPRAKGIAAKTVAGGLLVQSRDNGVVDD
LDLKVVTKRAPTEAELNDLKFAFRVGKHVKSNAIVYVKDGATVGIGAGQMSRVDSARIAARKAEDAAEAAGLAAPLTKGC
VVASDAFFPFADGLLSAVEAGATAVIQPGGSMRDDEVIAAADEHGIAMVMTGMRHFRH

Sequences:

>Translated_538_residues
MAVSSKHIPAPDLHRVRRALLSVSDKTGLIDFAKALHANGVEILSTGGTAKSIAAAGIPVKDVSEITGFPEIMDGRVKTL
HPAVHGGLLAVRNDPEHVAAMEEHGIGGIDLAVINLYPFEEVRFKGGDYDTTVENIDIGGPAMIRASAKNHAYVATVVDP
ADYADVVAELEKHSGSLPLAFRKKLAAKAFSRTAAYDAAISNWFAEAIDEETPTYRAVAGKLHSVMRYGENPHQTAGFYL
TGEKRPGVATATQLQGKQLSYNNINDTDAAFELVAEFDPARTAAVAIIKHANPCGVAEASTIKEAYLKALACDPVSAFGG
IVALNRTLDEEAAEEIVKTFTEVIIAPDATEGAQAIVAAKKNLRLLVTGGLPDPRAKGIAAKTVAGGLLVQSRDNGVVDD
LDLKVVTKRAPTEAELNDLKFAFRVGKHVKSNAIVYVKDGATVGIGAGQMSRVDSARIAARKAEDAAEAAGLAAPLTKGC
VVASDAFFPFADGLLSAVEAGATAVIQPGGSMRDDEVIAAADEHGIAMVMTGMRHFRH
>Mature_537_residues
AVSSKHIPAPDLHRVRRALLSVSDKTGLIDFAKALHANGVEILSTGGTAKSIAAAGIPVKDVSEITGFPEIMDGRVKTLH
PAVHGGLLAVRNDPEHVAAMEEHGIGGIDLAVINLYPFEEVRFKGGDYDTTVENIDIGGPAMIRASAKNHAYVATVVDPA
DYADVVAELEKHSGSLPLAFRKKLAAKAFSRTAAYDAAISNWFAEAIDEETPTYRAVAGKLHSVMRYGENPHQTAGFYLT
GEKRPGVATATQLQGKQLSYNNINDTDAAFELVAEFDPARTAAVAIIKHANPCGVAEASTIKEAYLKALACDPVSAFGGI
VALNRTLDEEAAEEIVKTFTEVIIAPDATEGAQAIVAAKKNLRLLVTGGLPDPRAKGIAAKTVAGGLLVQSRDNGVVDDL
DLKVVTKRAPTEAELNDLKFAFRVGKHVKSNAIVYVKDGATVGIGAGQMSRVDSARIAARKAEDAAEAAGLAAPLTKGCV
VASDAFFPFADGLLSAVEAGATAVIQPGGSMRDDEVIAAADEHGIAMVMTGMRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=471, Percent_Identity=40.7643312101911, Blast_Score=272, Evalue=5e-73,
Organism=Escherichia coli, GI1790439, Length=537, Percent_Identity=56.2383612662942, Blast_Score=544, Evalue=1e-156,
Organism=Caenorhabditis elegans, GI71985564, Length=475, Percent_Identity=35.3684210526316, Blast_Score=244, Evalue=7e-65,
Organism=Caenorhabditis elegans, GI71985574, Length=305, Percent_Identity=28.1967213114754, Blast_Score=95, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI71985556, Length=60, Percent_Identity=50, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323056, Length=607, Percent_Identity=34.7611202635914, Blast_Score=284, Evalue=3e-77,
Organism=Saccharomyces cerevisiae, GI6323768, Length=479, Percent_Identity=36.5344467640919, Blast_Score=257, Evalue=3e-69,
Organism=Drosophila melanogaster, GI24649832, Length=475, Percent_Identity=38.9473684210526, Blast_Score=261, Evalue=1e-69,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 56482; Mature: 56351

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVSSKHIPAPDLHRVRRALLSVSDKTGLIDFAKALHANGVEILSTGGTAKSIAAAGIPV
CCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEECCCCHHHHHHCCCCH
KDVSEITGFPEIMDGRVKTLHPAVHGGLLAVRNDPEHVAAMEEHGIGGIDLAVINLYPFE
HHHHHHCCCHHHHCCCHHEECHHHHCCEEEECCCHHHHHHHHHCCCCCEEEEEEEECCHH
EVRFKGGDYDTTVENIDIGGPAMIRASAKNHAYVATVVDPADYADVVAELEKHSGSLPLA
HHEECCCCCCCCCCEECCCCCEEEEECCCCCEEEEEEECCHHHHHHHHHHHHCCCCCCHH
FRKKLAAKAFSRTAAYDAAISNWFAEAIDEETPTYRAVAGKLHSVMRYGENPHQTAGFYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEE
TGEKRPGVATATQLQGKQLSYNNINDTDAAFELVAEFDPARTAAVAIIKHANPCGVAEAS
ECCCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHEEEEEECCCCCCCCHHH
TIKEAYLKALACDPVSAFGGIVALNRTLDEEAAEEIVKTFTEVIIAPDATEGAQAIVAAK
HHHHHHHHHHCCCCHHHHCCEEEECCHHHHHHHHHHHHHHHHEEECCCCCCCHHHHEEEC
KNLRLLVTGGLPDPRAKGIAAKTVAGGLLVQSRDNGVVDDLDLKVVTKRAPTEAELNDLK
CCCEEEEECCCCCCHHCCCCHHHHHCCEEEEECCCCCCCCCCEEEEECCCCCCCHHHHHH
FAFRVGKHVKSNAIVYVKDGATVGIGAGQMSRVDSARIAARKAEDAAEAAGLAAPLTKGC
HHHHHCCCCCCCEEEEEECCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCE
VVASDAFFPFADGLLSAVEAGATAVIQPGGSMRDDEVIAAADEHGIAMVMTGMRHFRH
EEECCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
AVSSKHIPAPDLHRVRRALLSVSDKTGLIDFAKALHANGVEILSTGGTAKSIAAAGIPV
CCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEECCCCHHHHHHCCCCH
KDVSEITGFPEIMDGRVKTLHPAVHGGLLAVRNDPEHVAAMEEHGIGGIDLAVINLYPFE
HHHHHHCCCHHHHCCCHHEECHHHHCCEEEECCCHHHHHHHHHCCCCCEEEEEEEECCHH
EVRFKGGDYDTTVENIDIGGPAMIRASAKNHAYVATVVDPADYADVVAELEKHSGSLPLA
HHEECCCCCCCCCCEECCCCCEEEEECCCCCEEEEEEECCHHHHHHHHHHHHCCCCCCHH
FRKKLAAKAFSRTAAYDAAISNWFAEAIDEETPTYRAVAGKLHSVMRYGENPHQTAGFYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEE
TGEKRPGVATATQLQGKQLSYNNINDTDAAFELVAEFDPARTAAVAIIKHANPCGVAEAS
ECCCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHEEEEEECCCCCCCCHHH
TIKEAYLKALACDPVSAFGGIVALNRTLDEEAAEEIVKTFTEVIIAPDATEGAQAIVAAK
HHHHHHHHHHCCCCHHHHCCEEEECCHHHHHHHHHHHHHHHHEEECCCCCCCHHHHEEEC
KNLRLLVTGGLPDPRAKGIAAKTVAGGLLVQSRDNGVVDDLDLKVVTKRAPTEAELNDLK
CCCEEEEECCCCCCHHCCCCHHHHHCCEEEEECCCCCCCCCCEEEEECCCCCCCHHHHHH
FAFRVGKHVKSNAIVYVKDGATVGIGAGQMSRVDSARIAARKAEDAAEAAGLAAPLTKGC
HHHHHCCCCCCCEEEEEECCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCE
VVASDAFFPFADGLLSAVEAGATAVIQPGGSMRDDEVIAAADEHGIAMVMTGMRHFRH
EEECCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA