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The map label for this gene is braE [H]

Identifier: 225853236

GI number: 225853236

Start: 1737683

End: 1739062

Strand: Reverse

Name: braE [H]

Synonym: BMEA_A1840

Alternate gene names: 225853236

Gene position: 1739062-1737683 (Counterclockwise)

Preceding gene: 225853237

Following gene: 225853235

Centisome position: 81.81

GC content: 57.9

Gene sequence:

>1380_bases
ATGGCAGTACAAACTGGTTCACGCCCGGATTCCCTTTTCGGCCGGGCTCTGCGCGAAGGGATCATGGCCGGTCTTCTGGC
TCTCGGTCTCTTCTGCCTCATCATCGGTTTCAAGACCGATCAGAACATCAACAACGAATTGGTGCTCGAACAGCGCTGGG
GAACGCTGGCCATCATGGTCGCGATCGCAGCGGTTGGGCGCTTCCTTTTCATCGCCTTTCTCCAGCCCTGGCTGGAGGCG
CGCAAGCTCGCCAAGAGCAGCCGGCCCGCGGTCTCAACCGAAAACCCTTCTTTTGTCCGCCGCAATTTCTCACGCCTTGG
CGTTGCCTTCCTCTTTGTCTATCCCGTTCTCATCGTTGCAGCCCTTGGCTGGCAGGGATCGTTGAAGTGGGTGGATAATT
TCGGTGTCCAGATCCTCATCTATGTGATGCTGGCCTGGGGCCTCAATATCGTGGTGGGTCTCGCCGGTCTGCTCGACCTT
GGCTATGTGGCCTTCTATGCGGTTGGCGCATATTCCTATGCCATTCTGTCCAGCTATTTCGGCCTGTCGTTCTGGGTGCT
TCTGCCCATCGCCGGTCTGCTCGCTGCGACATGGGGCATCATTCTGGGTTTCCCGGTGTTGCGCCTGCGCGGCGATTATC
TTGCAATCGTCACGCTTGCCTTCGGTGAAATCATCCGTCTGGTCCTCATCAACTGGACCGACCTTACCCGCGGCACCTTC
GGTATTTCCGGCATTGCCAAGGCAACCTTCTTCGGTCTGCCTTTCAATGCGAGCAAGGACGGTTTTGCCGCTCATTTCGG
CCTGGCCTTCTCAGCGGCGCATTATAAATTCTTTCTCTATTACGTCATTCTCCTGCTGGCCCTCCTGACGGCCTGGGTTA
CGATCCGCCTGCGCCGCATGCCGGTGGGGCGCGCGTGGGAAGCTTTGCGTGAAGACGAGATCGCCTGCCGTTCGCTTGGC
ATCAACACCACCACGACCAAGCTCACGGCGTTTGCCACGGGCGCGATGTTCGGTGGTTTTGCCGGTTCGTTCTTTGCCGC
ACGTCAGGGCTTCGTCAGCCCGGAATCCTTCGTGTTCCTCGAATCCGCAGTCATTCTGGCCATTGTGGTTCTGGGTGGCA
TGGGTTCGCTCGTTGGCATCGCGATTGCCGCGATTGTCATGATTGGCGGTACGGAAATCCTGCGTGAGATGAGCTTCCTG
AAGATAATCTTCGGGCCGGACTTTACGCCGGAACTCTATCGCATGTTGATCTTCGGCCTTGCCATGGTGGTTGTCATGGT
CTGGAAACCGCGTGGTTTCGTGGGAAGCCGTGAACCGAGCGCGTTCCTGAATGCGAGGAAGATGGTGTCAGGCGCCTTTA
CCAAGGAAGGGCACGGCTGA

Upstream 100 bases:

>100_bases
TCGACTACAAGGACGTCGCCGCGTTCTCGATCCTGGCTATCGTGCTGATCTTCATGCCGTCCGGCATTCTTGGCCGACCT
GAAGTCGAAAAGGTGTAATC

Downstream 100 bases:

>100_bases
TGGCGGAGACGAGCATTATGTCTGGTAATGGTTTGAAAGACACCATTCTCCAAGTCGAGCATCTTTCGATGCGCTTCGGT
GGCCTGATCGCCATCAATGA

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MAVQTGSRPDSLFGRALREGIMAGLLALGLFCLIIGFKTDQNINNELVLEQRWGTLAIMVAIAAVGRFLFIAFLQPWLEA
RKLAKSSRPAVSTENPSFVRRNFSRLGVAFLFVYPVLIVAALGWQGSLKWVDNFGVQILIYVMLAWGLNIVVGLAGLLDL
GYVAFYAVGAYSYAILSSYFGLSFWVLLPIAGLLAATWGIILGFPVLRLRGDYLAIVTLAFGEIIRLVLINWTDLTRGTF
GISGIAKATFFGLPFNASKDGFAAHFGLAFSAAHYKFFLYYVILLLALLTAWVTIRLRRMPVGRAWEALREDEIACRSLG
INTTTTKLTAFATGAMFGGFAGSFFAARQGFVSPESFVFLESAVILAIVVLGGMGSLVGIAIAAIVMIGGTEILREMSFL
KIIFGPDFTPELYRMLIFGLAMVVVMVWKPRGFVGSREPSAFLNARKMVSGAFTKEGHG

Sequences:

>Translated_459_residues
MAVQTGSRPDSLFGRALREGIMAGLLALGLFCLIIGFKTDQNINNELVLEQRWGTLAIMVAIAAVGRFLFIAFLQPWLEA
RKLAKSSRPAVSTENPSFVRRNFSRLGVAFLFVYPVLIVAALGWQGSLKWVDNFGVQILIYVMLAWGLNIVVGLAGLLDL
GYVAFYAVGAYSYAILSSYFGLSFWVLLPIAGLLAATWGIILGFPVLRLRGDYLAIVTLAFGEIIRLVLINWTDLTRGTF
GISGIAKATFFGLPFNASKDGFAAHFGLAFSAAHYKFFLYYVILLLALLTAWVTIRLRRMPVGRAWEALREDEIACRSLG
INTTTTKLTAFATGAMFGGFAGSFFAARQGFVSPESFVFLESAVILAIVVLGGMGSLVGIAIAAIVMIGGTEILREMSFL
KIIFGPDFTPELYRMLIFGLAMVVVMVWKPRGFVGSREPSAFLNARKMVSGAFTKEGHG
>Mature_458_residues
AVQTGSRPDSLFGRALREGIMAGLLALGLFCLIIGFKTDQNINNELVLEQRWGTLAIMVAIAAVGRFLFIAFLQPWLEAR
KLAKSSRPAVSTENPSFVRRNFSRLGVAFLFVYPVLIVAALGWQGSLKWVDNFGVQILIYVMLAWGLNIVVGLAGLLDLG
YVAFYAVGAYSYAILSSYFGLSFWVLLPIAGLLAATWGIILGFPVLRLRGDYLAIVTLAFGEIIRLVLINWTDLTRGTFG
ISGIAKATFFGLPFNASKDGFAAHFGLAFSAAHYKFFLYYVILLLALLTAWVTIRLRRMPVGRAWEALREDEIACRSLGI
NTTTTKLTAFATGAMFGGFAGSFFAARQGFVSPESFVFLESAVILAIVVLGGMGSLVGIAIAAIVMIGGTEILREMSFLK
IIFGPDFTPELYRMLIFGLAMVVVMVWKPRGFVGSREPSAFLNARKMVSGAFTKEGHG

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=307, Percent_Identity=55.3745928338762, Blast_Score=298, Evalue=6e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 50026; Mature: 49895

Theoretical pI: Translated: 10.27; Mature: 10.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AVQTGSRPDSLFGRALREGIMAGLLALGLFCLIIGFKTDQNINNELVLEQRWGTLAIMV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHH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HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]