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The map label for this gene is pgk [H]

Identifier: 225853183

GI number: 225853183

Start: 1687654

End: 1688844

Strand: Direct

Name: pgk [H]

Synonym: BMEA_A1782

Alternate gene names: 225853183

Gene position: 1687654-1688844 (Clockwise)

Preceding gene: 225853182

Following gene: 225853185

Centisome position: 79.39

GC content: 60.2

Gene sequence:

>1191_bases
ATGATGTTCCGCACCCTTGACGATGCCAATGTCCAATCCAAGCGCGTGCTGGTCCGTGTTGACCTCAACGTGCCGATGGC
AAACGGCGAAGTCACCGACCTTACCCGCATCGAACGCATCGTTCCGACCATCGCGGAACTGTCAAGGAAGGGCGCGAAGG
TCATCCTGCTCGCCCATTTCGGTCGCCCGAAGGGGGTTGCCTCGGATGAAAATTCGCTGAAGCATGTCGTGAAGCCGCTC
TCCAAGGTTCTGGATCACAGTGTTCATTTTGCCGAAGACTGCATCGGCGACAAGGCGAAAGCGGCCGTCGATGCGCTGAA
GGACGGCGACGTCCTGCTTCTGGAAAATACCCGTTTCCACAAGGGCGAGGAAAAGAACGATCCTGAATTCGTGCAGGCGC
TGGCCGCCAATGGCGATCTCTATGTGAACGATGCCTTTTCCGCCGCTCACCGCGCCCATGCCTCGACTGAAGGTCTGGCC
CATGTCCTGCCTGCCTTCGCGGGCCGCGCCATGCAGGCTGAGCTTGAAGCACTTGAAAAGGGCCTCGGCAATCCGGCCCG
CCCGGTGGTCGCCATCGTCGGCGGTGCAAAGGTTTCGACCAAGCTCGACCTCCTGTCCAACCTGATCGAAAAGGTCGATG
CGCTTGTCATCGGCGGCGGCATGGCCAACACGTTCCTTGCAGCCAAGGGTCTTGATGTCGGCAAATCGCTCTGCGAGCAT
GAGCTGGCCTCGACCGCACGCGAAATCATGGCCAAGGCCGAAACCACCAAATGCGCCATCATCCTGCCGGTGGACGCGGT
CGTGGGCTGGCACTTCGCCGCCGATACGCCACACCAGACCTATGGCGTGGATTCGGTTCCGGGCGACGCCATGATCCTTG
ACGCAGGCGAGCTTTCGACCGACCTCATTGCTTCGGCCATCGATGATGCCGCAACATTGGTCTGGAACGGCCCGCTTGGG
GCTTTCGAGTTGCGCCCCTTTGACACAGCAACGGTGAAGGTCGCAAGGCATGTCGCAAAGCGCACAAAGGAAGGCAAGCT
TGTTTCGGTCGGCGGCGGCGGCGATACGGTGGCAGCCCTCAACCATGCGGGCGTGGCCGACGACTTCACCTATATCTCAA
CCGCTGGCGGCGCTTTCCTCGAATGGATGGAAGGCAAACCGCTTCCGGGCGTCAATGTATTGAAGAAGTGA

Upstream 100 bases:

>100_bases
GACACCGCCGTCGCTTTGGGCAAGCTGATCTGATAACGGCAACGCCTCTCCTTCACTGGCGAGGCGTTTTCATTTCTTGA
TAAGGACCGAGAGAAGAAAC

Downstream 100 bases:

>100_bases
TACAATAAATCCCATCCACATAAGCCCGGTTGATGCCGGGCTCAGATTGATGAAAAACCCCTCGATTTTTGGCAGCAGTC
AAAAACGAGGGGTTTTGATT

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MMFRTLDDANVQSKRVLVRVDLNVPMANGEVTDLTRIERIVPTIAELSRKGAKVILLAHFGRPKGVASDENSLKHVVKPL
SKVLDHSVHFAEDCIGDKAKAAVDALKDGDVLLLENTRFHKGEEKNDPEFVQALAANGDLYVNDAFSAAHRAHASTEGLA
HVLPAFAGRAMQAELEALEKGLGNPARPVVAIVGGAKVSTKLDLLSNLIEKVDALVIGGGMANTFLAAKGLDVGKSLCEH
ELASTAREIMAKAETTKCAIILPVDAVVGWHFAADTPHQTYGVDSVPGDAMILDAGELSTDLIASAIDDAATLVWNGPLG
AFELRPFDTATVKVARHVAKRTKEGKLVSVGGGGDTVAALNHAGVADDFTYISTAGGAFLEWMEGKPLPGVNVLKK

Sequences:

>Translated_396_residues
MMFRTLDDANVQSKRVLVRVDLNVPMANGEVTDLTRIERIVPTIAELSRKGAKVILLAHFGRPKGVASDENSLKHVVKPL
SKVLDHSVHFAEDCIGDKAKAAVDALKDGDVLLLENTRFHKGEEKNDPEFVQALAANGDLYVNDAFSAAHRAHASTEGLA
HVLPAFAGRAMQAELEALEKGLGNPARPVVAIVGGAKVSTKLDLLSNLIEKVDALVIGGGMANTFLAAKGLDVGKSLCEH
ELASTAREIMAKAETTKCAIILPVDAVVGWHFAADTPHQTYGVDSVPGDAMILDAGELSTDLIASAIDDAATLVWNGPLG
AFELRPFDTATVKVARHVAKRTKEGKLVSVGGGGDTVAALNHAGVADDFTYISTAGGAFLEWMEGKPLPGVNVLKK
>Mature_396_residues
MMFRTLDDANVQSKRVLVRVDLNVPMANGEVTDLTRIERIVPTIAELSRKGAKVILLAHFGRPKGVASDENSLKHVVKPL
SKVLDHSVHFAEDCIGDKAKAAVDALKDGDVLLLENTRFHKGEEKNDPEFVQALAANGDLYVNDAFSAAHRAHASTEGLA
HVLPAFAGRAMQAELEALEKGLGNPARPVVAIVGGAKVSTKLDLLSNLIEKVDALVIGGGMANTFLAAKGLDVGKSLCEH
ELASTAREIMAKAETTKCAIILPVDAVVGWHFAADTPHQTYGVDSVPGDAMILDAGELSTDLIASAIDDAATLVWNGPLG
AFELRPFDTATVKVARHVAKRTKEGKLVSVGGGGDTVAALNHAGVADDFTYISTAGGAFLEWMEGKPLPGVNVLKK

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI4505763, Length=410, Percent_Identity=41.219512195122, Blast_Score=271, Evalue=5e-73,
Organism=Homo sapiens, GI31543397, Length=411, Percent_Identity=41.3625304136253, Blast_Score=267, Evalue=2e-71,
Organism=Escherichia coli, GI1789294, Length=398, Percent_Identity=43.4673366834171, Blast_Score=295, Evalue=4e-81,
Organism=Caenorhabditis elegans, GI17508823, Length=407, Percent_Identity=42.7518427518428, Blast_Score=293, Evalue=9e-80,
Organism=Saccharomyces cerevisiae, GI6319857, Length=408, Percent_Identity=38.9705882352941, Blast_Score=257, Evalue=2e-69,
Organism=Drosophila melanogaster, GI24581203, Length=410, Percent_Identity=40, Blast_Score=267, Evalue=9e-72,
Organism=Drosophila melanogaster, GI17136394, Length=408, Percent_Identity=38.7254901960784, Blast_Score=248, Evalue=5e-66,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 41795; Mature: 41795

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS00111 PGLYCERATE_KINASE ; PS00678 WD_REPEATS_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMFRTLDDANVQSKRVLVRVDLNVPMANGEVTDLTRIERIVPTIAELSRKGAKVILLAHF
CCEECCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEC
GRPKGVASDENSLKHVVKPLSKVLDHSVHFAEDCIGDKAKAAVDALKDGDVLLLENTRFH
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEECCCCC
KGEEKNDPEFVQALAANGDLYVNDAFSAAHRAHASTEGLAHVLPAFAGRAMQAELEALEK
CCCCCCCHHHHHHHHCCCCEEEECHHHHHHHHHCCHHHHHHHHHHHHCHHHHHHHHHHHH
GLGNPARPVVAIVGGAKVSTKLDLLSNLIEKVDALVIGGGMANTFLAAKGLDVGKSLCEH
CCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHCCCHHHHHHHHH
ELASTAREIMAKAETTKCAIILPVDAVVGWHFAADTPHQTYGVDSVPGDAMILDAGELST
HHHHHHHHHHHHHCCCEEEEEEEHHHHHEEEEECCCCCCCCCCCCCCCCEEEEECCCHHH
DLIASAIDDAATLVWNGPLGAFELRPFDTATVKVARHVAKRTKEGKLVSVGGGGDTVAAL
HHHHHHHCCCEEEEECCCCCCEEECCCCHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEC
NHAGVADDFTYISTAGGAFLEWMEGKPLPGVNVLKK
CCCCCCCCCCEEECCCCHHHHHHCCCCCCCCHHHCC
>Mature Secondary Structure
MMFRTLDDANVQSKRVLVRVDLNVPMANGEVTDLTRIERIVPTIAELSRKGAKVILLAHF
CCEECCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEC
GRPKGVASDENSLKHVVKPLSKVLDHSVHFAEDCIGDKAKAAVDALKDGDVLLLENTRFH
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEECCCCC
KGEEKNDPEFVQALAANGDLYVNDAFSAAHRAHASTEGLAHVLPAFAGRAMQAELEALEK
CCCCCCCHHHHHHHHCCCCEEEECHHHHHHHHHCCHHHHHHHHHHHHCHHHHHHHHHHHH
GLGNPARPVVAIVGGAKVSTKLDLLSNLIEKVDALVIGGGMANTFLAAKGLDVGKSLCEH
CCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHCCCHHHHHHHHH
ELASTAREIMAKAETTKCAIILPVDAVVGWHFAADTPHQTYGVDSVPGDAMILDAGELST
HHHHHHHHHHHHHCCCEEEEEEEHHHHHEEEEECCCCCCCCCCCCCCCCEEEEECCCHHH
DLIASAIDDAATLVWNGPLGAFELRPFDTATVKVARHVAKRTKEGKLVSVGGGGDTVAAL
HHHHHHHCCCEEEEECCCCCCEEECCCCHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEC
NHAGVADDFTYISTAGGAFLEWMEGKPLPGVNVLKK
CCCCCCCCCCEEECCCCHHHHHHCCCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA