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The map label for this gene is tolB

Identifier: 225853152

GI number: 225853152

Start: 1659927

End: 1661258

Strand: Reverse

Name: tolB

Synonym: BMEA_A1749

Alternate gene names: 225853152

Gene position: 1661258-1659927 (Counterclockwise)

Preceding gene: 225853153

Following gene: 225853151

Centisome position: 78.15

GC content: 59.08

Gene sequence:

>1332_bases
ATGAAAATCGGCATCATCAATACAAAGATCCGGACGGTCTTTTCGGCTTTCGCGTGTATGATTGCCGCAAGCCTCGTCTG
CACCATGCCGGCGCGCGCTGTCGTGGAAATCAACATCAACAAGGGTGTGATCGAGCCGCTGCCGATCGCCATCACCGATT
TTCTTTCGGCTGATCAGCTTGGATCGAATATCACGTCGGTCATTGCGGCCGATCTTGAGCGGTCCGGCCTCTTCGCCCCC
ATAGACAAGGGCGCTTTCATCGAAAAGATCTCCAATCCGGATGCGGCGCCGCGTTTCGAGGACTGGAAGGTCATCAATGC
GCAGGCGCTCGTTACGGGCCGCATCACCAAACAGCCCGATGGCAGGCTCAAGGCTGAGTTCCGTCTCTGGGATACATTTG
GCGGCCAGCAGATGATCGGCCAGCAATTTTTCACCACGCCGGATAACTGGCGTCGTGTTGCCCATATCATCGCGGACGCC
ATCTATGAGCGCCTGACCGGTGACAAAGGCTATTTCGATACCCGTGTCGTTTTCGTGGATGAATCAGGCCCGGCGCAGAA
GCGTGTGAAGCGCCTTGCCATCATGGACCAGGACGGCGCCAATGTGCGTTTTATTTCTGATGGCCGCGCCCTTTCGCTGA
CGCCGCGCTTCTCGCCGAACCGGCAGGAAGTGACCTATATGTCGTTTGAGGGCGGCTCGCCGAAGGTCTATCTGCTGCAA
CTTGAAACCGGCCAGCGCGAGCTTGTCGGCAACTTCCCCGGCATGACTATTGCGCCGCGTTTTTCGCCCGACGGCCAGAA
GGTCGTGATGAGCCTGCTGCAGGATGACGGCAGCGCCAATATCTATACGATGGATCTGCGCAATCGCACGACCACGCGCC
TGACCAGCAGCCAGGCGATCGATACGGGTGCTTCCTATTCGCCGGATGGTTCGCAGATTGTCTTCACATCCGACCGTGGC
GGGCGCCCGCAGCTCTATGTCATGGGTGCCGATGGCTCCAATCCGCGCCGTATCTCGATGGGTGACGGCAGCTATTCCAC
GCCGGTCTGGTCCCCGCGCGGTGATCTCATCGCTTTCACCAAGCAATCGCAGGGCCAGTTCTCCATTGGTGTGATGAAGA
CCGATGGTTCGGGTGAGCGTCTGCTGACATCGGGCTTTCACAATGAAGGCCCGACATGGGCCCCGAATGGCCGCGTGCTG
ATGTTTTTCCGCAAGGCAGCCGGTGCAGGCGGCCCGAAACTCTTCACCATCGACCTGACCGGCCGTAACGAACGCCAGAT
CCAGACGCCGAATTTTGCTTCCGACCCGGCCTGGTCGCCGCTGCTCGAATAG

Upstream 100 bases:

>100_bases
TGGTCGGAAGTGATCGTCAATTTCGACCCAAGCGAAATGTTCTGACCGGGGTTCCGGTCGCCAACCCATCACTGCCAAGG
ACAAAGAGGTCCCAGAAGAC

Downstream 100 bases:

>100_bases
CGATCATCGAAAAAATGACAGGGTTGAAGGCGTGAAGCTTCGGTTTCGCGCCTTTATTTATGGCTGATTGCCACAATCAG
GGGGGCAAAGCCGTGTTTCT

Product: translocation protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 443; Mature: 443

Protein sequence:

>443_residues
MKIGIINTKIRTVFSAFACMIAASLVCTMPARAVVEININKGVIEPLPIAITDFLSADQLGSNITSVIAADLERSGLFAP
IDKGAFIEKISNPDAAPRFEDWKVINAQALVTGRITKQPDGRLKAEFRLWDTFGGQQMIGQQFFTTPDNWRRVAHIIADA
IYERLTGDKGYFDTRVVFVDESGPAQKRVKRLAIMDQDGANVRFISDGRALSLTPRFSPNRQEVTYMSFEGGSPKVYLLQ
LETGQRELVGNFPGMTIAPRFSPDGQKVVMSLLQDDGSANIYTMDLRNRTTTRLTSSQAIDTGASYSPDGSQIVFTSDRG
GRPQLYVMGADGSNPRRISMGDGSYSTPVWSPRGDLIAFTKQSQGQFSIGVMKTDGSGERLLTSGFHNEGPTWAPNGRVL
MFFRKAAGAGGPKLFTIDLTGRNERQIQTPNFASDPAWSPLLE

Sequences:

>Translated_443_residues
MKIGIINTKIRTVFSAFACMIAASLVCTMPARAVVEININKGVIEPLPIAITDFLSADQLGSNITSVIAADLERSGLFAP
IDKGAFIEKISNPDAAPRFEDWKVINAQALVTGRITKQPDGRLKAEFRLWDTFGGQQMIGQQFFTTPDNWRRVAHIIADA
IYERLTGDKGYFDTRVVFVDESGPAQKRVKRLAIMDQDGANVRFISDGRALSLTPRFSPNRQEVTYMSFEGGSPKVYLLQ
LETGQRELVGNFPGMTIAPRFSPDGQKVVMSLLQDDGSANIYTMDLRNRTTTRLTSSQAIDTGASYSPDGSQIVFTSDRG
GRPQLYVMGADGSNPRRISMGDGSYSTPVWSPRGDLIAFTKQSQGQFSIGVMKTDGSGERLLTSGFHNEGPTWAPNGRVL
MFFRKAAGAGGPKLFTIDLTGRNERQIQTPNFASDPAWSPLLE
>Mature_443_residues
MKIGIINTKIRTVFSAFACMIAASLVCTMPARAVVEININKGVIEPLPIAITDFLSADQLGSNITSVIAADLERSGLFAP
IDKGAFIEKISNPDAAPRFEDWKVINAQALVTGRITKQPDGRLKAEFRLWDTFGGQQMIGQQFFTTPDNWRRVAHIIADA
IYERLTGDKGYFDTRVVFVDESGPAQKRVKRLAIMDQDGANVRFISDGRALSLTPRFSPNRQEVTYMSFEGGSPKVYLLQ
LETGQRELVGNFPGMTIAPRFSPDGQKVVMSLLQDDGSANIYTMDLRNRTTTRLTSSQAIDTGASYSPDGSQIVFTSDRG
GRPQLYVMGADGSNPRRISMGDGSYSTPVWSPRGDLIAFTKQSQGQFSIGVMKTDGSGERLLTSGFHNEGPTWAPNGRVL
MFFRKAAGAGGPKLFTIDLTGRNERQIQTPNFASDPAWSPLLE

Specific function: Involved in the tonB-independent uptake of proteins

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family

Homologues:

Organism=Escherichia coli, GI1786961, Length=434, Percent_Identity=30.4147465437788, Blast_Score=182, Evalue=3e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TOLB_BRUA1 (B2S7D4)

Other databases:

- EMBL:   CP000887
- RefSeq:   YP_001935555.1
- ProteinModelPortal:   B2S7D4
- SMR:   B2S7D4
- GeneID:   6327545
- GenomeReviews:   CP000887_GR
- KEGG:   bmc:BAbS19_I15970
- HOGENOM:   HBG644919
- OMA:   RVADYDG
- ProtClustDB:   PRK05137
- HAMAP:   MF_00671
- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943
- Gene3D:   G3DSA:3.40.50.10070
- Gene3D:   G3DSA:2.130.10.10
- TIGRFAMs:   TIGR02800

Pfam domain/function: PF07676 PD40; PF04052 TolB_N; SSF52964 TolB_N

EC number: NA

Molecular weight: Translated: 48529; Mature: 48529

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGIINTKIRTVFSAFACMIAASLVCTMPARAVVEININKGVIEPLPIAITDFLSADQL
CEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHCHHHH
GSNITSVIAADLERSGLFAPIDKGAFIEKISNPDAAPRFEDWKVINAQALVTGRITKQPD
CCHHHHHHHHHHHHCCCEEECCCCHHHHHCCCCCCCCCCCCEEEEEEEEEEEEEEECCCC
GRLKAEFRLWDTFGGQQMIGQQFFTTPDNWRRVAHIIADAIYERLTGDKGYFDTRVVFVD
CCEEEEEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEE
ESGPAQKRVKRLAIMDQDGANVRFISDGRALSLTPRFSPNRQEVTYMSFEGGSPKVYLLQ
CCCCHHHHHHHHEEECCCCCEEEEEECCCEEEEECCCCCCCCEEEEEEECCCCCEEEEEE
LETGQRELVGNFPGMTIAPRFSPDGQKVVMSLLQDDGSANIYTMDLRNRTTTRLTSSQAI
ECCCCHHHHCCCCCCEEECCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCEEEEECCCCC
DTGASYSPDGSQIVFTSDRGGRPQLYVMGADGSNPRRISMGDGSYSTPVWSPRGDLIAFT
CCCCCCCCCCCEEEEECCCCCCCEEEEEECCCCCCCEEEECCCCCCCCCCCCCCCEEEEE
KQSQGQFSIGVMKTDGSGERLLTSGFHNEGPTWAPNGRVLMFFRKAAGAGGPKLFTIDLT
ECCCCEEEEEEEEECCCCCEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEEEC
GRNERQIQTPNFASDPAWSPLLE
CCCCCEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MKIGIINTKIRTVFSAFACMIAASLVCTMPARAVVEININKGVIEPLPIAITDFLSADQL
CEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHCHHHH
GSNITSVIAADLERSGLFAPIDKGAFIEKISNPDAAPRFEDWKVINAQALVTGRITKQPD
CCHHHHHHHHHHHHCCCEEECCCCHHHHHCCCCCCCCCCCCEEEEEEEEEEEEEEECCCC
GRLKAEFRLWDTFGGQQMIGQQFFTTPDNWRRVAHIIADAIYERLTGDKGYFDTRVVFVD
CCEEEEEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEE
ESGPAQKRVKRLAIMDQDGANVRFISDGRALSLTPRFSPNRQEVTYMSFEGGSPKVYLLQ
CCCCHHHHHHHHEEECCCCCEEEEEECCCEEEEECCCCCCCCEEEEEEECCCCCEEEEEE
LETGQRELVGNFPGMTIAPRFSPDGQKVVMSLLQDDGSANIYTMDLRNRTTTRLTSSQAI
ECCCCHHHHCCCCCCEEECCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCEEEEECCCCC
DTGASYSPDGSQIVFTSDRGGRPQLYVMGADGSNPRRISMGDGSYSTPVWSPRGDLIAFT
CCCCCCCCCCCEEEEECCCCCCCEEEEEECCCCCCCEEEECCCCCCCCCCCCCCCEEEEE
KQSQGQFSIGVMKTDGSGERLLTSGFHNEGPTWAPNGRVLMFFRKAAGAGGPKLFTIDLT
ECCCCEEEEEEEEECCCCCEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEEEC
GRNERQIQTPNFASDPAWSPLLE
CCCCCEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA