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The map label for this gene is alkJ [H]

Identifier: 225853103

GI number: 225853103

Start: 1602253

End: 1603914

Strand: Direct

Name: alkJ [H]

Synonym: BMEA_A1687

Alternate gene names: 225853103

Gene position: 1602253-1603914 (Clockwise)

Preceding gene: 225853101

Following gene: 225853104

Centisome position: 75.38

GC content: 56.62

Gene sequence:

>1662_bases
ATGCTGCTCCCCGAACATCCCACGTCGCAGCTTGGGAGGAGTAAAATGGAAAAATTCGATTATATCATAATCGGGGCCGG
ATCATCCGGCTGCGCGCTGGCGAAGGGGCTGACTGAGAACGCCACGAATACCGTCCTTCTGCTTGAAGCAGGCCCCCATG
CTGACCGGTTCTGGGTCAACACGCCTGCTGGCATGGCCAAGCTTTTTTTTCACGATGTATTGAATTGGAACTATTATACC
GAGCCTATGGCGAGGCTAAAAGGCCGCAAGATGTACTGGCCTCGCGGTAAGCTTCTCGGAGGATCGAGTCCCATAAATGG
CATGGTGTTTATTCGCGGACACCCAAGCGACTTCGACCCATGGCGCGATCTTGGCAATCCCGGCTGGGGTTATCAGGACG
TTCTACCCTATTTCAAGGCAATGGAGCACTTTGAACGGCGTAGCGATGTCTGGCGCGGTCAGGGTGGCCCCTTGTGGATC
AGCGATCCCGTCGTCAAGGAGCCTGCGAGCTACGATTTCATAGAGACATGCAGGCGCCTCGGCCACCTTGAAACGGAGGA
CATGAACGGCGAGCAACATGACGGTGTGGGCTTCATGCAGCACACAATCAAGAATGGCCGCCGTCACTCGGCCTACCGCT
CCTTCGTAAAACCAATTCTGAAACGGTCAAATCTGACCGTGCGAACGAATGCACATGTGCAACGCATCCTGTTCGACGGC
AAAACGGCGGTAGGTGTTGAAGTCCTGCTTGACGGAGAACTCCACAGAATATTGGCGGCCCGTGAAGTCATCCTCTCGGG
AGGCTCGATCAACTCACCACAGCTCCTGATGCTATCGGGCGTCGGTCCCGCCGCAGAGGTGACACGTCACGATATCCCGC
TGATTCTGGATTTGCCGGGCGTCGGCCGGAACTTACTTGATCATTTCTGCGTGCATACAGGCTGGCGATCAACGCCGGGC
AGCTCCTACAACGCGAACCTAAGGGGGCTGCGAAAATACTGGGAAGGCTTCAAATATCTCATGGCACATAAGGGGCATTT
GGCGCTTGGTTCGTCCCAGGTCGCCGCCTTCGTCAAAAGCTCGCCGGAAGAGCCCTATGCGGACCTCCAGATCAGTTTTC
GCCCCATGACATTCAACTATTTTCCAGATGGCCGTATGGAGGTGGAGGATTTCCCGGGCTTGGGCGTGTCGATTTTCCAA
TTGCGCCCCAACGCGCAGGGCACGGTAACATTGCGCTCCCCGCGCGCAACAGACAAGGCTGTCTGCACGCCCCAGTGTAT
GACGGACGCTTACGACATTGCAGCAATGATGAGCGGCATCAAGCAGGTCCGGCAAATCATGAATTCGGAACCTATCAAGT
CGCGTGTCGTCGCCGAAGAGGTTCCCGGTCCGGACGTTCAGAGCGACGACGAAATCTTCCGTTTCATGGAAGAGACCGGC
AATACCGCCCAGCACACTGCCGGAACGTGCAAGATGGGCAAGGCCCCCATGGCCGTTGTCGATGAGCGCCTCAGGGTTCG
AGGGATCGAGCGTCTTCGAGTGGTAGATGCCTCCGTCATGCCGAAGCTGACATCCGGGAACACGAACGCTCCGGCGATCA
TGATCGGCGTTAAGGGCGCGGATATGATTTGCAAGGATGCGGTACCGCGCCGGGAATTTTAA

Upstream 100 bases:

>100_bases
AAAGCCTATAAGAAGACACTCCTCGGAACATTTGTATGGTAACATCACTGGCTCTCAGATCGGAAAGATAAGGGAGGGCC
TCGCCGCTATCGTATAATCC

Downstream 100 bases:

>100_bases
GGCGAACGAATAGCGGTCGAAACCACCGCCTCTCAAAATCCGGCTGGATCAAAGACAGGAGGGAGATCACATCGAGCACT
TCCGAAAACTTTGTGGTTAG

Product: choline dehydrogenase

Products: Betaine Aldehyde; Reduced Acceptor. [C]

Alternate protein names: NA

Number of amino acids: Translated: 553; Mature: 553

Protein sequence:

>553_residues
MLLPEHPTSQLGRSKMEKFDYIIIGAGSSGCALAKGLTENATNTVLLLEAGPHADRFWVNTPAGMAKLFFHDVLNWNYYT
EPMARLKGRKMYWPRGKLLGGSSPINGMVFIRGHPSDFDPWRDLGNPGWGYQDVLPYFKAMEHFERRSDVWRGQGGPLWI
SDPVVKEPASYDFIETCRRLGHLETEDMNGEQHDGVGFMQHTIKNGRRHSAYRSFVKPILKRSNLTVRTNAHVQRILFDG
KTAVGVEVLLDGELHRILAAREVILSGGSINSPQLLMLSGVGPAAEVTRHDIPLILDLPGVGRNLLDHFCVHTGWRSTPG
SSYNANLRGLRKYWEGFKYLMAHKGHLALGSSQVAAFVKSSPEEPYADLQISFRPMTFNYFPDGRMEVEDFPGLGVSIFQ
LRPNAQGTVTLRSPRATDKAVCTPQCMTDAYDIAAMMSGIKQVRQIMNSEPIKSRVVAEEVPGPDVQSDDEIFRFMEETG
NTAQHTAGTCKMGKAPMAVVDERLRVRGIERLRVVDASVMPKLTSGNTNAPAIMIGVKGADMICKDAVPRREF

Sequences:

>Translated_553_residues
MLLPEHPTSQLGRSKMEKFDYIIIGAGSSGCALAKGLTENATNTVLLLEAGPHADRFWVNTPAGMAKLFFHDVLNWNYYT
EPMARLKGRKMYWPRGKLLGGSSPINGMVFIRGHPSDFDPWRDLGNPGWGYQDVLPYFKAMEHFERRSDVWRGQGGPLWI
SDPVVKEPASYDFIETCRRLGHLETEDMNGEQHDGVGFMQHTIKNGRRHSAYRSFVKPILKRSNLTVRTNAHVQRILFDG
KTAVGVEVLLDGELHRILAAREVILSGGSINSPQLLMLSGVGPAAEVTRHDIPLILDLPGVGRNLLDHFCVHTGWRSTPG
SSYNANLRGLRKYWEGFKYLMAHKGHLALGSSQVAAFVKSSPEEPYADLQISFRPMTFNYFPDGRMEVEDFPGLGVSIFQ
LRPNAQGTVTLRSPRATDKAVCTPQCMTDAYDIAAMMSGIKQVRQIMNSEPIKSRVVAEEVPGPDVQSDDEIFRFMEETG
NTAQHTAGTCKMGKAPMAVVDERLRVRGIERLRVVDASVMPKLTSGNTNAPAIMIGVKGADMICKDAVPRREF
>Mature_553_residues
MLLPEHPTSQLGRSKMEKFDYIIIGAGSSGCALAKGLTENATNTVLLLEAGPHADRFWVNTPAGMAKLFFHDVLNWNYYT
EPMARLKGRKMYWPRGKLLGGSSPINGMVFIRGHPSDFDPWRDLGNPGWGYQDVLPYFKAMEHFERRSDVWRGQGGPLWI
SDPVVKEPASYDFIETCRRLGHLETEDMNGEQHDGVGFMQHTIKNGRRHSAYRSFVKPILKRSNLTVRTNAHVQRILFDG
KTAVGVEVLLDGELHRILAAREVILSGGSINSPQLLMLSGVGPAAEVTRHDIPLILDLPGVGRNLLDHFCVHTGWRSTPG
SSYNANLRGLRKYWEGFKYLMAHKGHLALGSSQVAAFVKSSPEEPYADLQISFRPMTFNYFPDGRMEVEDFPGLGVSIFQ
LRPNAQGTVTLRSPRATDKAVCTPQCMTDAYDIAAMMSGIKQVRQIMNSEPIKSRVVAEEVPGPDVQSDDEIFRFMEETG
NTAQHTAGTCKMGKAPMAVVDERLRVRGIERLRVVDASVMPKLTSGNTNAPAIMIGVKGADMICKDAVPRREF

Specific function: Converts aliphatic medium-chain-length alcohols into aldehydes. May be linked to the electron transfer chain [H]

COG id: COG2303

COG function: function code E; Choline dehydrogenase and related flavoproteins

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GMC oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI217272839, Length=541, Percent_Identity=39.9260628465804, Blast_Score=389, Evalue=1e-108,
Organism=Escherichia coli, GI1786503, Length=543, Percent_Identity=38.6740331491713, Blast_Score=318, Evalue=7e-88,
Organism=Caenorhabditis elegans, GI17532301, Length=552, Percent_Identity=36.7753623188406, Blast_Score=342, Evalue=2e-94,
Organism=Drosophila melanogaster, GI24642059, Length=584, Percent_Identity=35.445205479452, Blast_Score=302, Evalue=5e-82,
Organism=Drosophila melanogaster, GI24642048, Length=565, Percent_Identity=35.2212389380531, Blast_Score=301, Evalue=7e-82,
Organism=Drosophila melanogaster, GI45551458, Length=573, Percent_Identity=33.8568935427574, Blast_Score=296, Evalue=3e-80,
Organism=Drosophila melanogaster, GI45549471, Length=573, Percent_Identity=33.8568935427574, Blast_Score=296, Evalue=3e-80,
Organism=Drosophila melanogaster, GI24642042, Length=577, Percent_Identity=34.315424610052, Blast_Score=295, Evalue=7e-80,
Organism=Drosophila melanogaster, GI24650267, Length=569, Percent_Identity=34.622144112478, Blast_Score=285, Evalue=5e-77,
Organism=Drosophila melanogaster, GI17137792, Length=546, Percent_Identity=34.4322344322344, Blast_Score=282, Evalue=4e-76,
Organism=Drosophila melanogaster, GI24642055, Length=584, Percent_Identity=33.2191780821918, Blast_Score=279, Evalue=3e-75,
Organism=Drosophila melanogaster, GI24642039, Length=572, Percent_Identity=34.2657342657343, Blast_Score=276, Evalue=4e-74,
Organism=Drosophila melanogaster, GI18859995, Length=576, Percent_Identity=34.5486111111111, Blast_Score=275, Evalue=5e-74,
Organism=Drosophila melanogaster, GI24642037, Length=570, Percent_Identity=33.5087719298246, Blast_Score=268, Evalue=1e-71,
Organism=Drosophila melanogaster, GI24642035, Length=576, Percent_Identity=33.3333333333333, Blast_Score=259, Evalue=3e-69,
Organism=Drosophila melanogaster, GI24642051, Length=573, Percent_Identity=30.5410122164049, Blast_Score=243, Evalue=3e-64,
Organism=Drosophila melanogaster, GI18859993, Length=572, Percent_Identity=31.6433566433566, Blast_Score=237, Evalue=2e-62,
Organism=Drosophila melanogaster, GI24645930, Length=581, Percent_Identity=28.3993115318417, Blast_Score=158, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24642057, Length=591, Percent_Identity=28.7648054145516, Blast_Score=141, Evalue=9e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012132
- InterPro:   IPR000172
- InterPro:   IPR007867 [H]

Pfam domain/function: PF05199 GMC_oxred_C; PF00732 GMC_oxred_N [H]

EC number: 1.1.99.1 [C]

Molecular weight: Translated: 61485; Mature: 61485

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: PS00623 GMC_OXRED_1 ; PS00624 GMC_OXRED_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLPEHPTSQLGRSKMEKFDYIIIGAGSSGCALAKGLTENATNTVLLLEAGPHADRFWVN
CCCCCCCHHHHHHHHHHHCCEEEEECCCCCCHHHCCCCCCCCCEEEEEECCCCCCEEEEE
TPAGMAKLFFHDVLNWNYYTEPMARLKGRKMYWPRGKLLGGSSPINGMVFIRGHPSDFDP
CCHHHHHHHHHHHHCCCCCCCHHHHHCCCEEECCCCCCCCCCCCCCCEEEEECCCCCCCH
WRDLGNPGWGYQDVLPYFKAMEHFERRSDVWRGQGGPLWISDPVVKEPASYDFIETCRRL
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCHHHHHHHH
GHLETEDMNGEQHDGVGFMQHTIKNGRRHSAYRSFVKPILKRSNLTVRTNAHVQRILFDG
CCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEECCCEEEEEECC
KTAVGVEVLLDGELHRILAAREVILSGGSINSPQLLMLSGVGPAAEVTRHDIPLILDLPG
CCEEEEEEEECCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHCCCCEEEECCC
VGRNLLDHFCVHTGWRSTPGSSYNANLRGLRKYWEGFKYLMAHKGHLALGSSQVAAFVKS
CCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHC
SPEEPYADLQISFRPMTFNYFPDGRMEVEDFPGLGVSIFQLRPNAQGTVTLRSPRATDKA
CCCCCCCCEEEEEEEEEEEECCCCCEECCCCCCCCEEEEEECCCCCEEEEEECCCCCCCC
VCTPQCMTDAYDIAAMMSGIKQVRQIMNSEPIKSRVVAEEVPGPDVQSDDEIFRFMEETG
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCC
NTAQHTAGTCKMGKAPMAVVDERLRVRGIERLRVVDASVMPKLTSGNTNAPAIMIGVKGA
CCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHEEECHHHCCCCCCCCCCCCEEEEEECCC
DMICKDAVPRREF
CHHHHCCCCCCCC
>Mature Secondary Structure
MLLPEHPTSQLGRSKMEKFDYIIIGAGSSGCALAKGLTENATNTVLLLEAGPHADRFWVN
CCCCCCCHHHHHHHHHHHCCEEEEECCCCCCHHHCCCCCCCCCEEEEEECCCCCCEEEEE
TPAGMAKLFFHDVLNWNYYTEPMARLKGRKMYWPRGKLLGGSSPINGMVFIRGHPSDFDP
CCHHHHHHHHHHHHCCCCCCCHHHHHCCCEEECCCCCCCCCCCCCCCEEEEECCCCCCCH
WRDLGNPGWGYQDVLPYFKAMEHFERRSDVWRGQGGPLWISDPVVKEPASYDFIETCRRL
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCHHHHHHHH
GHLETEDMNGEQHDGVGFMQHTIKNGRRHSAYRSFVKPILKRSNLTVRTNAHVQRILFDG
CCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEECCCEEEEEECC
KTAVGVEVLLDGELHRILAAREVILSGGSINSPQLLMLSGVGPAAEVTRHDIPLILDLPG
CCEEEEEEEECCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHCCCCEEEECCC
VGRNLLDHFCVHTGWRSTPGSSYNANLRGLRKYWEGFKYLMAHKGHLALGSSQVAAFVKS
CCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHC
SPEEPYADLQISFRPMTFNYFPDGRMEVEDFPGLGVSIFQLRPNAQGTVTLRSPRATDKA
CCCCCCCCEEEEEEEEEEEECCCCCEECCCCCCCCEEEEEECCCCCEEEEEECCCCCCCC
VCTPQCMTDAYDIAAMMSGIKQVRQIMNSEPIKSRVVAEEVPGPDVQSDDEIFRFMEETG
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCC
NTAQHTAGTCKMGKAPMAVVDERLRVRGIERLRVVDASVMPKLTSGNTNAPAIMIGVKGA
CCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHEEECHHHCCCCCCCCCCCCEEEEEECCC
DMICKDAVPRREF
CHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Coenzyme Q [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 7 {choline}} 1.1 {phenazine}} [C]

Substrates: Choline; Acceptor [C]

Specific reaction: Choline + Acceptor = Betaine Aldehyde + Reduced Acceptor. [C]

General reaction: Redox reaction [C]

Inhibitor: 2-Dimethylaminoethanol; Betaine aldehyde; Monoethanolamine; Semicarbazide [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11390693 [H]