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The map label for this gene is potH [H]

Identifier: 225853082

GI number: 225853082

Start: 1576814

End: 1577725

Strand: Reverse

Name: potH [H]

Synonym: BMEA_A1663

Alternate gene names: 225853082

Gene position: 1577725-1576814 (Counterclockwise)

Preceding gene: 225853083

Following gene: 225853081

Centisome position: 74.22

GC content: 56.36

Gene sequence:

>912_bases
ATGCAAAGGGTGATCGCTTCCATCGCCAGCCGCCTTGTCATCGCAGTGCCATATCTCTGGCTGCTGGTGTTCTTCTTGGT
GCCGTTCTTCATCGTCTTCAAGATTTCCCTGTCGGAAGTCGCGATGGCGATCCCGGCCTATGCGCCGACCTTCGATCTGG
CACGGGGATTTGCGGCGAACTGGGAGAAAATAAAGCAGCTCTCCTTCGATAATTATCTGTGGCTGCTTGACGATCCGCTC
TATTACAAGGCCTATCTGTCGAGCGTCCGCATTGCCGCCATTTCCACTGTTCTGACGCTGATTGTGGCCTATCCGATGGC
CTATGGCATTGCGCGCGCGCCAGCCACCATCCGTCCGACATTGTTGATGATGGTTATCCTGCCGTTCTGGACCTCGTTCC
TCATCCGCGTCTATGCGTGGATCGGTATTTTGAAGCCGGAAGGGCTGCTCAATCAGTTCCTCATGGGGCTGAACATCATC
GATACGCCGCTCAATATCCTGAACACCGATACGGCGGTGTTCATCGGTATCGTCTATTCCTATCTGCCCTTCATGGTGCT
GCCGATCTATTCGTCTTTGGAAAAGATGGATTATTCGCTGATCGAGGCGGCGCAGGATCTGGGCTGCACACCCTTTGCCG
CCTTCTGGAAGATCACCTTTCCGTTGTCGCTGGCGGGTGTTCTGGCCGGCTGCTTCCTCGTCTTCATCCCGGCGGTGGGC
GAATTCGTCATCCCGGATCTTCTGGGTGGATCACAGACGCTGATGATCGGCAAGACGATCTGGAGCGAGTTCTTCTCCAA
CCGCGACTGGCCGGTCTCCTCGGCGGTGGCGGTGGTGCTGCTCGTCCTGCTGATTGTGCCGATCGTCATTTTCCAGCAGG
TGCAGGCGCGGGCACAGGAAAAGGGGAAATAG

Upstream 100 bases:

>100_bases
GCGCAAGACTGAAAACCCGCTCACCTATGACGAGCGCGTCTGGGTTTCATTCGATATCGATGCCGGTCTGGTGCTGACGG
CCTGAGGAAAGGGCAAGCTT

Downstream 100 bases:

>100_bases
GATGCAGAACAACTGGTCCCGCTTCAATATCGTATCGGTGGCGCTGGGTTTTGCCTTTCTCTACCTGCCCATCGTGCTTT
TGGTGATTTATTCGTTCAAT

Product: binding-protein-dependent transport systems inner membrane protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MQRVIASIASRLVIAVPYLWLLVFFLVPFFIVFKISLSEVAMAIPAYAPTFDLARGFAANWEKIKQLSFDNYLWLLDDPL
YYKAYLSSVRIAAISTVLTLIVAYPMAYGIARAPATIRPTLLMMVILPFWTSFLIRVYAWIGILKPEGLLNQFLMGLNII
DTPLNILNTDTAVFIGIVYSYLPFMVLPIYSSLEKMDYSLIEAAQDLGCTPFAAFWKITFPLSLAGVLAGCFLVFIPAVG
EFVIPDLLGGSQTLMIGKTIWSEFFSNRDWPVSSAVAVVLLVLLIVPIVIFQQVQARAQEKGK

Sequences:

>Translated_303_residues
MQRVIASIASRLVIAVPYLWLLVFFLVPFFIVFKISLSEVAMAIPAYAPTFDLARGFAANWEKIKQLSFDNYLWLLDDPL
YYKAYLSSVRIAAISTVLTLIVAYPMAYGIARAPATIRPTLLMMVILPFWTSFLIRVYAWIGILKPEGLLNQFLMGLNII
DTPLNILNTDTAVFIGIVYSYLPFMVLPIYSSLEKMDYSLIEAAQDLGCTPFAAFWKITFPLSLAGVLAGCFLVFIPAVG
EFVIPDLLGGSQTLMIGKTIWSEFFSNRDWPVSSAVAVVLLVLLIVPIVIFQQVQARAQEKGK
>Mature_303_residues
MQRVIASIASRLVIAVPYLWLLVFFLVPFFIVFKISLSEVAMAIPAYAPTFDLARGFAANWEKIKQLSFDNYLWLLDDPL
YYKAYLSSVRIAAISTVLTLIVAYPMAYGIARAPATIRPTLLMMVILPFWTSFLIRVYAWIGILKPEGLLNQFLMGLNII
DTPLNILNTDTAVFIGIVYSYLPFMVLPIYSSLEKMDYSLIEAAQDLGCTPFAAFWKITFPLSLAGVLAGCFLVFIPAVG
EFVIPDLLGGSQTLMIGKTIWSEFFSNRDWPVSSAVAVVLLVLLIVPIVIFQQVQARAQEKGK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=295, Percent_Identity=55.5932203389831, Blast_Score=306, Evalue=1e-84,
Organism=Escherichia coli, GI1787369, Length=234, Percent_Identity=43.1623931623932, Blast_Score=209, Evalue=1e-55,
Organism=Escherichia coli, GI1787713, Length=235, Percent_Identity=28.936170212766, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1787368, Length=122, Percent_Identity=35.2459016393443, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 33831; Mature: 33831

Theoretical pI: Translated: 8.13; Mature: 8.13

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRVIASIASRLVIAVPYLWLLVFFLVPFFIVFKISLSEVAMAIPAYAPTFDLARGFAAN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCC
WEKIKQLSFDNYLWLLDDPLYYKAYLSSVRIAAISTVLTLIVAYPMAYGIARAPATIRPT
HHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
LLMMVILPFWTSFLIRVYAWIGILKPEGLLNQFLMGLNIIDTPLNILNTDTAVFIGIVYS
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHCCHHHHCCHHHHHHHHHHH
YLPFMVLPIYSSLEKMDYSLIEAAQDLGCTPFAAFWKITFPLSLAGVLAGCFLVFIPAVG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EFVIPDLLGGSQTLMIGKTIWSEFFSNRDWPVSSAVAVVLLVLLIVPIVIFQQVQARAQE
HHHHHHHCCCCCCEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KGK
CCC
>Mature Secondary Structure
MQRVIASIASRLVIAVPYLWLLVFFLVPFFIVFKISLSEVAMAIPAYAPTFDLARGFAAN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCC
WEKIKQLSFDNYLWLLDDPLYYKAYLSSVRIAAISTVLTLIVAYPMAYGIARAPATIRPT
HHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
LLMMVILPFWTSFLIRVYAWIGILKPEGLLNQFLMGLNIIDTPLNILNTDTAVFIGIVYS
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHCCHHHHCCHHHHHHHHHHH
YLPFMVLPIYSSLEKMDYSLIEAAQDLGCTPFAAFWKITFPLSLAGVLAGCFLVFIPAVG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EFVIPDLLGGSQTLMIGKTIWSEFFSNRDWPVSSAVAVVLLVLLIVPIVIFQQVQARAQE
HHHHHHHCCCCCCEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KGK
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]