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The map label for this gene is leuA [H]

Identifier: 225853043

GI number: 225853043

Start: 1536146

End: 1537873

Strand: Reverse

Name: leuA [H]

Synonym: BMEA_A1620

Alternate gene names: 225853043

Gene position: 1537873-1536146 (Counterclockwise)

Preceding gene: 225853044

Following gene: 225853041

Centisome position: 72.35

GC content: 57.52

Gene sequence:

>1728_bases
ATGAACCAGCCTGTCTCCACGCCAAAGTCCGTGTCCCAAGCCGTATCGGCACGCAAGGGCATGCCTATTGCCGGTACCAA
ATACGCGCCGTTCCCTGCGCCGCAGCTCGATGACCGCACTTGGCCTTCCAAACGCATCGAAAAGGCGCCGATCTGGTGTT
CGGTCGATCTGCGCGACGGCAACCAGGCTTTGATCGACCCCATGGGGCATGACCGCAAGGAGCGCATGTTCCGCCTGCTG
ATCGATATGGGTTTCCCGGAAATCGAGATCGGTTTCCCGTCCGCTTCGCAGACGGATTTCGACTTCTGCCGCTGGGCCAT
TGAACAGGGCGATGTTCCTGACGACGTGGATTTGCAGGTTCTGGTCCAGTGCCGTCCTGAATTGATTACGCGCACTTTCG
AGGCGCTGGAAGGCGCGAAGACGCCGATCATCCATTTCTACAATTCCACCAGCGAATTGCAGCGCCGCGTGGTGTTTGCC
AAGGATGTCGGCGGCATCAAGCAGATTGCAACCGATGCGGCCAAGATGATCATGGATATGGCTGCCAAGGCTGGCGGCGG
TTATCGCTTCCAGTATTCGCCGGAAAGCTTTACCGGCACCGAACTGGATGTGGCGCTGGAAATCTGCAATGCGGTGATCG
AGATCGTGAAGCCGACGCCGGACAACAAGCTGATCGTCAACCTGCCTTCGACGGTGGAGATGAACACGCCGAATGTCTAT
GCCGACCAGATCGAATGGATGTGCCGCAATCTGGACAATCGCGAGAGCCTGATTATCTCGCTGCATCCGCATAATGACCG
CGGCACGGGCATTGCCGCCACTGAACTGGGCCTGATGGCAGGCGCTGATCGCGTGGAAGGCACGCTGTTCGGCAATGGCG
AGCGCACGGGCAATGTGGATGTGGTGACGCTGGCGCTCAACATGTACACGCAGGGCATCGACCCCGGGCTGGATTGCACC
GATATCAACCGGATGAAGGAAGTCTACGAATATTCCAACCAGTTGAAGATCGCCGAGCGCCATCCTTATGTGGGCGAGCT
GGTCTATACGGCTTTCTCCGGCTCGCATCAGGATGCGATCAACAAGGGCATGAAAGCGCGCCGTTCGGCCAATTCGCCGG
TTTGGGAAGTTCCTTACCTGCCGATCGATCCGCAGGATGTGGGCCGCTCCTACGAGGCGATCATCCGCATCAATTCGCAA
TCGGGCAAGGGCGGTATCGCCTATATCCTGCAAGCCGATTACGGCCTGAACCTGCCGCGCAACCTGCAGGTCGAGTTCCG
CGAAATCATCCAGCACATTACGGATGAAGAGGGCAAGGAGCTGCCATCGAAGCGCATTTACGAGGAGTTCCAGAAACTCT
ATGTGACGCAGCCCGATGCCCGCATCAAGTTCGTGGATCACCACACCTATCCCCATCCTGAGCAAAAGGGCCGGCGCATC
CTGACGGCTGAGATCACCGATAACGGTGTCACCAAGACTATCGAGGGCAAGGGCACGGGACCGATCGATGGTTTCGTGGA
TGCGCTGTCGAAATATCTTGGCGTGAAAATGTCGGTGGTCGATTATTCCGAACATTCGCTCCAGCAGGGTTCGGATGCTT
CGGCCATTTCCTATGTCGAGATGGTTTATCCCGGCGGCAAGCTGTTCGGTGTCGGCATCAACGATAATATCGTCAGCGCC
TCGCTTGAAGCCGTGGTGTCGGCGGCAAATCGTGTGATTGCGAAGTAG

Upstream 100 bases:

>100_bases
GCCGGACCTGTGCTCCTTTCCAGAAAACCCATATAATCGATCAGCATTTGTCCGCGCTTGACGGTATGGAACGCCGTTGA
AAGCCGGGAGGAAACGAGAA

Downstream 100 bases:

>100_bases
AGCTACAGTTGCAAATTCGTCAGGCCGTGGCGAAAAGAGTGATTTTCGAGTACCGGAGCGCAGCGTATTTAAGGCTACGT
GAGCACCGGAACGCAGATAG

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 575; Mature: 575

Protein sequence:

>575_residues
MNQPVSTPKSVSQAVSARKGMPIAGTKYAPFPAPQLDDRTWPSKRIEKAPIWCSVDLRDGNQALIDPMGHDRKERMFRLL
IDMGFPEIEIGFPSASQTDFDFCRWAIEQGDVPDDVDLQVLVQCRPELITRTFEALEGAKTPIIHFYNSTSELQRRVVFA
KDVGGIKQIATDAAKMIMDMAAKAGGGYRFQYSPESFTGTELDVALEICNAVIEIVKPTPDNKLIVNLPSTVEMNTPNVY
ADQIEWMCRNLDNRESLIISLHPHNDRGTGIAATELGLMAGADRVEGTLFGNGERTGNVDVVTLALNMYTQGIDPGLDCT
DINRMKEVYEYSNQLKIAERHPYVGELVYTAFSGSHQDAINKGMKARRSANSPVWEVPYLPIDPQDVGRSYEAIIRINSQ
SGKGGIAYILQADYGLNLPRNLQVEFREIIQHITDEEGKELPSKRIYEEFQKLYVTQPDARIKFVDHHTYPHPEQKGRRI
LTAEITDNGVTKTIEGKGTGPIDGFVDALSKYLGVKMSVVDYSEHSLQQGSDASAISYVEMVYPGGKLFGVGINDNIVSA
SLEAVVSAANRVIAK

Sequences:

>Translated_575_residues
MNQPVSTPKSVSQAVSARKGMPIAGTKYAPFPAPQLDDRTWPSKRIEKAPIWCSVDLRDGNQALIDPMGHDRKERMFRLL
IDMGFPEIEIGFPSASQTDFDFCRWAIEQGDVPDDVDLQVLVQCRPELITRTFEALEGAKTPIIHFYNSTSELQRRVVFA
KDVGGIKQIATDAAKMIMDMAAKAGGGYRFQYSPESFTGTELDVALEICNAVIEIVKPTPDNKLIVNLPSTVEMNTPNVY
ADQIEWMCRNLDNRESLIISLHPHNDRGTGIAATELGLMAGADRVEGTLFGNGERTGNVDVVTLALNMYTQGIDPGLDCT
DINRMKEVYEYSNQLKIAERHPYVGELVYTAFSGSHQDAINKGMKARRSANSPVWEVPYLPIDPQDVGRSYEAIIRINSQ
SGKGGIAYILQADYGLNLPRNLQVEFREIIQHITDEEGKELPSKRIYEEFQKLYVTQPDARIKFVDHHTYPHPEQKGRRI
LTAEITDNGVTKTIEGKGTGPIDGFVDALSKYLGVKMSVVDYSEHSLQQGSDASAISYVEMVYPGGKLFGVGINDNIVSA
SLEAVVSAANRVIAK
>Mature_575_residues
MNQPVSTPKSVSQAVSARKGMPIAGTKYAPFPAPQLDDRTWPSKRIEKAPIWCSVDLRDGNQALIDPMGHDRKERMFRLL
IDMGFPEIEIGFPSASQTDFDFCRWAIEQGDVPDDVDLQVLVQCRPELITRTFEALEGAKTPIIHFYNSTSELQRRVVFA
KDVGGIKQIATDAAKMIMDMAAKAGGGYRFQYSPESFTGTELDVALEICNAVIEIVKPTPDNKLIVNLPSTVEMNTPNVY
ADQIEWMCRNLDNRESLIISLHPHNDRGTGIAATELGLMAGADRVEGTLFGNGERTGNVDVVTLALNMYTQGIDPGLDCT
DINRMKEVYEYSNQLKIAERHPYVGELVYTAFSGSHQDAINKGMKARRSANSPVWEVPYLPIDPQDVGRSYEAIIRINSQ
SGKGGIAYILQADYGLNLPRNLQVEFREIIQHITDEEGKELPSKRIYEEFQKLYVTQPDARIKFVDHHTYPHPEQKGRRI
LTAEITDNGVTKTIEGKGTGPIDGFVDALSKYLGVKMSVVDYSEHSLQQGSDASAISYVEMVYPGGKLFGVGINDNIVSA
SLEAVVSAANRVIAK

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=532, Percent_Identity=23.6842105263158, Blast_Score=96, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6324225, Length=563, Percent_Identity=46.7140319715808, Blast_Score=526, Evalue=1e-150,
Organism=Saccharomyces cerevisiae, GI6324682, Length=565, Percent_Identity=47.0796460176991, Blast_Score=517, Evalue=1e-147,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005668
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 63711; Mature: 63711

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQPVSTPKSVSQAVSARKGMPIAGTKYAPFPAPQLDDRTWPSKRIEKAPIWCSVDLRDG
CCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCEEEEEEECCC
NQALIDPMGHDRKERMFRLLIDMGFPEIEIGFPSASQTDFDFCRWAIEQGDVPDDVDLQV
CEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCHHH
LVQCRPELITRTFEALEGAKTPIIHFYNSTSELQRRVVFAKDVGGIKQIATDAAKMIMDM
HECCCHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
AAKAGGGYRFQYSPESFTGTELDVALEICNAVIEIVKPTPDNKLIVNLPSTVEMNTPNVY
HHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEECCCCCH
ADQIEWMCRNLDNRESLIISLHPHNDRGTGIAATELGLMAGADRVEGTLFGNGERTGNVD
HHHHHHHHHCCCCCCEEEEEEECCCCCCCCEEHHHHHHHCCCCCCCEEEEECCCCCCCEE
VVTLALNMYTQGIDPGLDCTDINRMKEVYEYSNQLKIAERHPYVGELVYTAFSGSHQDAI
EEEEEEEHHHCCCCCCCCCHHHHHHHHHHHHHCCEEEECCCCCHHHHHEEHHCCCCHHHH
NKGMKARRSANSPVWEVPYLPIDPQDVGRSYEAIIRINSQSGKGGIAYILQADYGLNLPR
HHHHHHHHCCCCCEEECCCCCCCHHHHCCCEEEEEEEECCCCCCCEEEEEEECCCCCCCC
NLQVEFREIIQHITDEEGKELPSKRIYEEFQKLYVTQPDARIKFVDHHTYPHPEQKGRRI
CCCHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHEECCCCCEEEEEECCCCCCCHHHCCEE
LTAEITDNGVTKTIEGKGTGPIDGFVDALSKYLGVKMSVVDYSEHSLQQGSDASAISYVE
EEEEECCCCCEEEECCCCCCCHHHHHHHHHHHHCCEEEEEECCHHHHCCCCCCCHHEEEE
MVYPGGKLFGVGINDNIVSASLEAVVSAANRVIAK
EEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNQPVSTPKSVSQAVSARKGMPIAGTKYAPFPAPQLDDRTWPSKRIEKAPIWCSVDLRDG
CCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCEEEEEEECCC
NQALIDPMGHDRKERMFRLLIDMGFPEIEIGFPSASQTDFDFCRWAIEQGDVPDDVDLQV
CEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCHHH
LVQCRPELITRTFEALEGAKTPIIHFYNSTSELQRRVVFAKDVGGIKQIATDAAKMIMDM
HECCCHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
AAKAGGGYRFQYSPESFTGTELDVALEICNAVIEIVKPTPDNKLIVNLPSTVEMNTPNVY
HHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEECCCCCH
ADQIEWMCRNLDNRESLIISLHPHNDRGTGIAATELGLMAGADRVEGTLFGNGERTGNVD
HHHHHHHHHCCCCCCEEEEEEECCCCCCCCEEHHHHHHHCCCCCCCEEEEECCCCCCCEE
VVTLALNMYTQGIDPGLDCTDINRMKEVYEYSNQLKIAERHPYVGELVYTAFSGSHQDAI
EEEEEEEHHHCCCCCCCCCHHHHHHHHHHHHHCCEEEECCCCCHHHHHEEHHCCCCHHHH
NKGMKARRSANSPVWEVPYLPIDPQDVGRSYEAIIRINSQSGKGGIAYILQADYGLNLPR
HHHHHHHHCCCCCEEECCCCCCCHHHHCCCEEEEEEEECCCCCCCEEEEEEECCCCCCCC
NLQVEFREIIQHITDEEGKELPSKRIYEEFQKLYVTQPDARIKFVDHHTYPHPEQKGRRI
CCCHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHEECCCCCEEEEEECCCCCCCHHHCCEE
LTAEITDNGVTKTIEGKGTGPIDGFVDALSKYLGVKMSVVDYSEHSLQQGSDASAISYVE
EEEEECCCCCEEEECCCCCCCHHHHHHHHHHHHCCEEEEEECCHHHHCCCCCCCHHEEEE
MVYPGGKLFGVGINDNIVSASLEAVVSAANRVIAK
EEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]