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The map label for this gene is pncA [H]

Identifier: 225852944

GI number: 225852944

Start: 1441798

End: 1442427

Strand: Direct

Name: pncA [H]

Synonym: BMEA_A1514

Alternate gene names: 225852944

Gene position: 1441798-1442427 (Clockwise)

Preceding gene: 225852942

Following gene: 225852946

Centisome position: 67.83

GC content: 56.03

Gene sequence:

>630_bases
ATGATCGGCCATGCACTTGTGGTTATCGACGTCCAGAATGATTTTTGCCCCGGCGGCGCCTTGGCCGTCGAACGTGGCGA
TGAAATCATCCCGATCGTCAACCGCCTGATCGGGGAAAGCGAAAATGTCGTGGTCACGCAGGATTGGCATCCGGCCAACC
ATTCCAGCTTCGCCTCCAACCACTCCGGCAGCCGCCCGTTCGATACGGTCAATATGTCTTATGGGCCGCAGGTGCTCTGG
CCGGATCATTGCGTGCAAAATAGCGAGGGGGCAGAATTTCACCCCGATCTGCAATGGTGGCGTGCACAGCTTGTCGTCCG
CAAGGGTTTCCGCATTGGTATCGACAGCTACTCCGCATTTTTTGAAAATGACCATTGCACGCCGACGGGCCTTGGCGGAT
ATTTGCGTGAACGCAATATCGGATCATTGACAATGGTTGGCCTCGCCACCGATTTCTGCGTCGCCAGTTCAGCGCTTGAT
GCGGTCCAGCAGGGCTTTCAGGTGCGGGTGCGACTCGATGCCTGCCGCGGTATTGATCTCAACGGGTCGATGAACATCAT
GCTGGAGAAAATGAAACAGGCTGGCGTCGAACTGATCGATGAGCCTGTTTCAGCGTCGCATCTACTCTAG

Upstream 100 bases:

>100_bases
TATCACCAATGCCTTTGTTATACCAGAACGTGCATATTTGCCGGTTTCCCAAAAACTGGATGGATATAAGTTGCTCGGCA
ACAACAAAGCGGAGCATCCC

Downstream 100 bases:

>100_bases
GTCACTTCCTCGGTCTCGTTCTGCCAGTTCGGGTTCAGGCGGTTATAGCGCAGCGACGACATGAAGGAGAGGCCGATAAG
TGCCGCGCCGATGAGGCCGG

Product: nicotinamidase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MIGHALVVIDVQNDFCPGGALAVERGDEIIPIVNRLIGESENVVVTQDWHPANHSSFASNHSGSRPFDTVNMSYGPQVLW
PDHCVQNSEGAEFHPDLQWWRAQLVVRKGFRIGIDSYSAFFENDHCTPTGLGGYLRERNIGSLTMVGLATDFCVASSALD
AVQQGFQVRVRLDACRGIDLNGSMNIMLEKMKQAGVELIDEPVSASHLL

Sequences:

>Translated_209_residues
MIGHALVVIDVQNDFCPGGALAVERGDEIIPIVNRLIGESENVVVTQDWHPANHSSFASNHSGSRPFDTVNMSYGPQVLW
PDHCVQNSEGAEFHPDLQWWRAQLVVRKGFRIGIDSYSAFFENDHCTPTGLGGYLRERNIGSLTMVGLATDFCVASSALD
AVQQGFQVRVRLDACRGIDLNGSMNIMLEKMKQAGVELIDEPVSASHLL
>Mature_209_residues
MIGHALVVIDVQNDFCPGGALAVERGDEIIPIVNRLIGESENVVVTQDWHPANHSSFASNHSGSRPFDTVNMSYGPQVLW
PDHCVQNSEGAEFHPDLQWWRAQLVVRKGFRIGIDSYSAFFENDHCTPTGLGGYLRERNIGSLTMVGLATDFCVASSALD
AVQQGFQVRVRLDACRGIDLNGSMNIMLEKMKQAGVELIDEPVSASHLL

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=204, Percent_Identity=47.0588235294118, Blast_Score=179, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI193206735, Length=219, Percent_Identity=36.0730593607306, Blast_Score=109, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI17543130, Length=221, Percent_Identity=35.7466063348416, Blast_Score=109, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI71999339, Length=224, Percent_Identity=31.6964285714286, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6321401, Length=215, Percent_Identity=32.093023255814, Blast_Score=119, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24648247, Length=201, Percent_Identity=34.8258706467662, Blast_Score=119, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 22936; Mature: 22936

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGHALVVIDVQNDFCPGGALAVERGDEIIPIVNRLIGESENVVVTQDWHPANHSSFASN
CCCCEEEEEECCCCCCCCCEEEECCCCCCHHHHHHHHCCCCCEEEEECCCCCCCCCCCCC
HSGSRPFDTVNMSYGPQVLWPDHCVQNSEGAEFHPDLQWWRAQLVVRKGFRIGIDSYSAF
CCCCCCCEEEECCCCCEEECCHHHCCCCCCCCCCCCHHHHHHHHHHHCCCEECCCHHHHH
FENDHCTPTGLGGYLRERNIGSLTMVGLATDFCVASSALDAVQQGFQVRVRLDACRGIDL
HCCCCCCCCCCCCHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCC
NGSMNIMLEKMKQAGVELIDEPVSASHLL
CCCHHHHHHHHHHCCHHHHCCCCCCCCCC
>Mature Secondary Structure
MIGHALVVIDVQNDFCPGGALAVERGDEIIPIVNRLIGESENVVVTQDWHPANHSSFASN
CCCCEEEEEECCCCCCCCCEEEECCCCCCHHHHHHHHCCCCCEEEEECCCCCCCCCCCCC
HSGSRPFDTVNMSYGPQVLWPDHCVQNSEGAEFHPDLQWWRAQLVVRKGFRIGIDSYSAF
CCCCCCCEEEECCCCCEEECCHHHCCCCCCCCCCCCHHHHHHHHHHHCCCEECCCHHHHH
FENDHCTPTGLGGYLRERNIGSLTMVGLATDFCVASSALDAVQQGFQVRVRLDACRGIDL
HCCCCCCCCCCCCHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCC
NGSMNIMLEKMKQAGVELIDEPVSASHLL
CCCHHHHHHHHHHCCHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]