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The map label for this gene is cit [H]

Identifier: 225852934

GI number: 225852934

Start: 1431514

End: 1432839

Strand: Reverse

Name: cit [H]

Synonym: BMEA_A1503

Alternate gene names: 225852934

Gene position: 1432839-1431514 (Counterclockwise)

Preceding gene: 225852936

Following gene: 225852932

Centisome position: 67.41

GC content: 57.16

Gene sequence:

>1326_bases
ATGCATGTTGACGCCAATGGCGAGGTCGAGGCGCCGCACGATGCCCACGATACGCGCCGCCGTGTTTATGCAATCGTAGC
CAGTGCCTCGGGCAACCTCGTGGAATGGTATGATTTTTACGTCTATTCCTTTGGTGCGCTTTATTTCGCGTCGCAATTCT
TCCCGGCTGAAGATCAGACCAGTCAATTGCTGAATGCTGCGGCCATTTTCGCGGCGGGCTTCCTGATGCGCCCCATCGGC
GGCTGGCTCTTCGGCCGCATCGCGGACAAGCTCGGCCGCCGCCTGTCCATGCTGGTTTCGGTTACGATGATGTGTTTCGG
CTCCTTCGCGATCGCGATCCTGCCAACCTATGAAACAATCGGCGTTCTGGCGCCTTTCCTGCTTCTGGTGGTGCGCCTCG
TTCAGGGGCTTTCAGTCGGCGGTGAATATGGCACCACAGCCACTTATATGAGCGAGGTTGCTCTGGCCGGGCGCCGTGGC
TTCTTCTCGTCTTTCCAGTATGTGACGCTGATCGGTGGCCAGCTTCTGGCCGTTCTGGTGATTGTCGTGCTGCAATTCCT
GCTCACGTCCGAGCAGCTTCATTCCTGGGGCTGGCGCATTCCTTTCGCCATTGGCGGCCTTGCGGCCATCGTGGCTCTTT
ACCTGCGCCGCACGCTGCATGAAACCTCGACGGAAAAGACCCGTGGCAACAAGGCGGCGGGCAGCTTCTCCAACATCTGG
AAGAACCATCGCAAGGCTTTCCTCGTTGTCGTGGGCTTCACGGCTGGCGGCTCGCTCTCCTTCTATACTTTCACCACTTA
TATGCAGAAATATCTGGTGAATACGGCGGGGATGAGCAAGGAAACGGCCAGTGAAACGATGACCTTCGTGCTTCTGGTCT
TCATGCTGATGCAGCCACTTTTCGGCGCGCTGTCTGACAAGGTCGGCCGTAAGACCATGATGATGGGCTTTGGCGGTATT
TCGATCCTGACCACAATCCCGCTCATGACGGTTATCGGCTCGGTGCAAAGCTCTACCTGGGCGTTTATCTATATCGTGAT
CGCACTTGCGGTGGTCAGCATGTACACTTCCGTCAGCGGCATTGTGAAGGCGGAGCTTTTCCCGGCGGAAGTGCGGGCGC
TGGGTGTCGGGTTTTCCTACGCCATCGCCAATGCACTTTTCGGCGGCACGGCGGAATATGTCGCTCTCTGGTTCAAGAAG
CAGGGAATGGAAAGCGGCTTCTTCTGGTATGTGACGGCCATTATGGTGGTGGTCTTTATCGTGAGCCTCCTCATGCCGAA
CCCGCGTGAACATGGCTATTTGCAGGGGCATGGTTCGGACGATTGA

Upstream 100 bases:

>100_bases
AACAATCGTCGCTTTTGATAATCCCCGTATGGGATAGACGGCTTGCAGGCTGTTGCGGAACCGCCTGTTATCGGGAGTTT
TCAGAATGAACAATGCAGGA

Downstream 100 bases:

>100_bases
TGTTGCCAGACCTTTCAGCTTGTTATGCTTCAGCGCCGCCTTCGGGCGGCGTTGTCACATCTCTACCGCTTCGTCGTCTT
CCGGGGACAGCAGCCGTGTT

Product: metabolite:proton symporter family protein

Products: Proton [Cytoplasm]; 2-oxoglutarate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MHVDANGEVEAPHDAHDTRRRVYAIVASASGNLVEWYDFYVYSFGALYFASQFFPAEDQTSQLLNAAAIFAAGFLMRPIG
GWLFGRIADKLGRRLSMLVSVTMMCFGSFAIAILPTYETIGVLAPFLLLVVRLVQGLSVGGEYGTTATYMSEVALAGRRG
FFSSFQYVTLIGGQLLAVLVIVVLQFLLTSEQLHSWGWRIPFAIGGLAAIVALYLRRTLHETSTEKTRGNKAAGSFSNIW
KNHRKAFLVVVGFTAGGSLSFYTFTTYMQKYLVNTAGMSKETASETMTFVLLVFMLMQPLFGALSDKVGRKTMMMGFGGI
SILTTIPLMTVIGSVQSSTWAFIYIVIALAVVSMYTSVSGIVKAELFPAEVRALGVGFSYAIANALFGGTAEYVALWFKK
QGMESGFFWYVTAIMVVVFIVSLLMPNPREHGYLQGHGSDD

Sequences:

>Translated_441_residues
MHVDANGEVEAPHDAHDTRRRVYAIVASASGNLVEWYDFYVYSFGALYFASQFFPAEDQTSQLLNAAAIFAAGFLMRPIG
GWLFGRIADKLGRRLSMLVSVTMMCFGSFAIAILPTYETIGVLAPFLLLVVRLVQGLSVGGEYGTTATYMSEVALAGRRG
FFSSFQYVTLIGGQLLAVLVIVVLQFLLTSEQLHSWGWRIPFAIGGLAAIVALYLRRTLHETSTEKTRGNKAAGSFSNIW
KNHRKAFLVVVGFTAGGSLSFYTFTTYMQKYLVNTAGMSKETASETMTFVLLVFMLMQPLFGALSDKVGRKTMMMGFGGI
SILTTIPLMTVIGSVQSSTWAFIYIVIALAVVSMYTSVSGIVKAELFPAEVRALGVGFSYAIANALFGGTAEYVALWFKK
QGMESGFFWYVTAIMVVVFIVSLLMPNPREHGYLQGHGSDD
>Mature_441_residues
MHVDANGEVEAPHDAHDTRRRVYAIVASASGNLVEWYDFYVYSFGALYFASQFFPAEDQTSQLLNAAAIFAAGFLMRPIG
GWLFGRIADKLGRRLSMLVSVTMMCFGSFAIAILPTYETIGVLAPFLLLVVRLVQGLSVGGEYGTTATYMSEVALAGRRG
FFSSFQYVTLIGGQLLAVLVIVVLQFLLTSEQLHSWGWRIPFAIGGLAAIVALYLRRTLHETSTEKTRGNKAAGSFSNIW
KNHRKAFLVVVGFTAGGSLSFYTFTTYMQKYLVNTAGMSKETASETMTFVLLVFMLMQPLFGALSDKVGRKTMMMGFGGI
SILTTIPLMTVIGSVQSSTWAFIYIVIALAVVSMYTSVSGIVKAELFPAEVRALGVGFSYAIANALFGGTAEYVALWFKK
QGMESGFFWYVTAIMVVVFIVSLLMPNPREHGYLQGHGSDD

Specific function: Uptake of alpha-ketoglutarate across the boundary membrane with the concomitant import of a cation (symport system) [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788942, Length=416, Percent_Identity=64.6634615384615, Blast_Score=518, Evalue=1e-148,
Organism=Escherichia coli, GI1790550, Length=417, Percent_Identity=29.7362110311751, Blast_Score=201, Evalue=7e-53,
Organism=Escherichia coli, GI1788292, Length=383, Percent_Identity=31.8537859007833, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1789941, Length=405, Percent_Identity=30.1234567901235, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI87082231, Length=310, Percent_Identity=28.0645161290323, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI87082404, Length=327, Percent_Identity=25.9938837920489, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 48256; Mature: 48256

Theoretical pI: Translated: 9.19; Mature: 9.19

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHVDANGEVEAPHDAHDTRRRVYAIVASASGNLVEWYDFYVYSFGALYFASQFFPAEDQT
CCCCCCCCCCCCCCHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHH
SQLLNAAAIFAAGFLMRPIGGWLFGRIADKLGRRLSMLVSVTMMCFGSFAIAILPTYETI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHH
GVLAPFLLLVVRLVQGLSVGGEYGTTATYMSEVALAGRRGFFSSFQYVTLIGGQLLAVLV
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
IVVLQFLLTSEQLHSWGWRIPFAIGGLAAIVALYLRRTLHETSTEKTRGNKAAGSFSNIW
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCHHHHH
KNHRKAFLVVVGFTAGGSLSFYTFTTYMQKYLVNTAGMSKETASETMTFVLLVFMLMQPL
HHCCEEEEEEEEECCCCCEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
FGALSDKVGRKTMMMGFGGISILTTIPLMTVIGSVQSSTWAFIYIVIALAVVSMYTSVSG
HHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
IVKAELFPAEVRALGVGFSYAIANALFGGTAEYVALWFKKQGMESGFFWYVTAIMVVVFI
HHHHHCCHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
VSLLMPNPREHGYLQGHGSDD
HHHHCCCCCCCCEECCCCCCC
>Mature Secondary Structure
MHVDANGEVEAPHDAHDTRRRVYAIVASASGNLVEWYDFYVYSFGALYFASQFFPAEDQT
CCCCCCCCCCCCCCHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHH
SQLLNAAAIFAAGFLMRPIGGWLFGRIADKLGRRLSMLVSVTMMCFGSFAIAILPTYETI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHH
GVLAPFLLLVVRLVQGLSVGGEYGTTATYMSEVALAGRRGFFSSFQYVTLIGGQLLAVLV
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
IVVLQFLLTSEQLHSWGWRIPFAIGGLAAIVALYLRRTLHETSTEKTRGNKAAGSFSNIW
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCHHHHH
KNHRKAFLVVVGFTAGGSLSFYTFTTYMQKYLVNTAGMSKETASETMTFVLLVFMLMQPL
HHCCEEEEEEEEECCCCCEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
FGALSDKVGRKTMMMGFGGISILTTIPLMTVIGSVQSSTWAFIYIVIALAVVSMYTSVSG
HHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
IVKAELFPAEVRALGVGFSYAIANALFGGTAEYVALWFKKQGMESGFFWYVTAIMVVVFI
HHHHHCCHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
VSLLMPNPREHGYLQGHGSDD
HHHHCCCCCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 2-oxoglutarate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 2-oxoglutarate [Periplasm] = Proton [Cytoplasm] + 2-oxoglutarate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2118499; 9205837; 9278503; 1709493; 2190187; 2053984; 8419306 [H]