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The map label for this gene is murF [H]

Identifier: 225852917

GI number: 225852917

Start: 1413335

End: 1414780

Strand: Reverse

Name: murF [H]

Synonym: BMEA_A1483

Alternate gene names: 225852917

Gene position: 1414780-1413335 (Counterclockwise)

Preceding gene: 225852918

Following gene: 225852916

Centisome position: 66.56

GC content: 59.06

Gene sequence:

>1446_bases
ATGAGCAATCGGGAATTGAGCGATTGGCTCTGGACATCCGACGCCATGGTGGAAGCCATGGAAGGCAGGCCGTTCGGCAA
TCTGCCGGCGGGAGTAACCGGCATTTCCATCGATAGCCGCACCCTGAAGCCCGGTGAGGCTTTTTTCGCCATCAAGGGCG
AACAGTTCGACGGCCACGATTTTGCAACGGCCGCCATGGCGGCAGGCGCGGGGCTTCTGGTTGTGGCGGAACATCGCCTG
CCTGCTTTCGGCAATACAAAAGTGCCGATGATCGTGGTCGAGGACGTGCTGGAAGCCCTGATCAGGCTTGGCATCGCTGC
GCGCGCGCGCTCGAAGGCGCAGATCATCGCCGTGACGGGTTCGGTCGGCAAGACAACCACCAAGGAAGCCCTGCGCCATG
TTCTGTCCGATGTCGGCAAGGTTCATGCTTCCGCCGCCTCGTTCAACAATCATTGGGGCGTGCCGCTGACACTTGCCCGT
ATGCCCGCCGATGTGGATTACGGCGTGTTCGAGATCGGCATGAACCATCATGGCGAAATCCGTCCGCTGGTGAAGATGGT
GCGCCCGCATGTCGCGCTGATTACGCTGATTGCACCGGCGCATCTTGGCCATTTTGCCAATCTGGAAGAGATTGCGGTTG
CCAAGGCGGAGATTTTCGAGGGCATCGTGCCTGGTGGCTATGCGCTTTTGAACCGCGACGACAAGCGCTTCAGGCAATTG
CAGGAACTGGCCGAAAAGGCCGGGGTCGAACATATTGCGACTTTCGGCGAGAATGCGCGTGCCGATTATCGCCTGCGCGA
TGTGAAGCTTCATCCAACCTGTTCGTGCATGACGGTGAAGATCGGAACGCATGAGGCAGCGGTGAAGGTGGGAATACCGG
GCCGCCATGTCGTGCAGAACATGCTTGCCGTTCTCGGCGCCGCGCACCTTGTCGGTGCGGATATTGCCAAGGTTATGATG
GCGATGGCGACGCTTTCTGCCCAGGGCGGGCGCGGTGCACGTCATGTGCTTCAACATCCGGAAGGCGCTTTCACGCTGAT
CGATGAAAGCTACAATGCCAATCCGACCTCGATGCGCGCGGCCCTTTCGCTGCTGCATTCGACCGTACCGGAAGGATCGC
GCGGGCGGCGTATTGCGGTTCTGGGCGATATGGTGGAACTTGGCCGACAGTCCGGCAAGCTGCATGCAGACCTTGCGCGT
CCCATTGTGGACGCGGAAGTGAATACACTTTTTATCGGCGGATCGGAGATGTCTGTGCTGAAAAATGCCTTGCCGGTTGA
AATTCAGACCGAATACAGGCAGAGCACCGACGAATTATTGCCGCTTGTGGTCAAGGCGGTCCGTCCCGGCGATGTCGTCA
TGGTGAAATCGTCCAACAGGACCGGCTTCTCCAAAATCGTCAAGGCGCTGATTGCCCAGTTTCCGCCCGTAGAAGCGGCG
GAATAG

Upstream 100 bases:

>100_bases
GACATGAGGAAGGGCAGATCGTCGGCAATATTACGCTGCCGTTTTCAGATCATGCAGAAGTGGCGGCGGCACTTGCCGCC
CGTCTTGAGGAGCATCTAAA

Downstream 100 bases:

>100_bases
GCGATTATTGAGGGATTGTTTTTCACATGCTGATGTTTCTCACCCATTTCGCCGAACATGTGACACCGTTCAATGTGTTC
CGCTACATTACCTTCCGCAC

Product: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MSNRELSDWLWTSDAMVEAMEGRPFGNLPAGVTGISIDSRTLKPGEAFFAIKGEQFDGHDFATAAMAAGAGLLVVAEHRL
PAFGNTKVPMIVVEDVLEALIRLGIAARARSKAQIIAVTGSVGKTTTKEALRHVLSDVGKVHASAASFNNHWGVPLTLAR
MPADVDYGVFEIGMNHHGEIRPLVKMVRPHVALITLIAPAHLGHFANLEEIAVAKAEIFEGIVPGGYALLNRDDKRFRQL
QELAEKAGVEHIATFGENARADYRLRDVKLHPTCSCMTVKIGTHEAAVKVGIPGRHVVQNMLAVLGAAHLVGADIAKVMM
AMATLSAQGGRGARHVLQHPEGAFTLIDESYNANPTSMRAALSLLHSTVPEGSRGRRIAVLGDMVELGRQSGKLHADLAR
PIVDAEVNTLFIGGSEMSVLKNALPVEIQTEYRQSTDELLPLVVKAVRPGDVVMVKSSNRTGFSKIVKALIAQFPPVEAA
E

Sequences:

>Translated_481_residues
MSNRELSDWLWTSDAMVEAMEGRPFGNLPAGVTGISIDSRTLKPGEAFFAIKGEQFDGHDFATAAMAAGAGLLVVAEHRL
PAFGNTKVPMIVVEDVLEALIRLGIAARARSKAQIIAVTGSVGKTTTKEALRHVLSDVGKVHASAASFNNHWGVPLTLAR
MPADVDYGVFEIGMNHHGEIRPLVKMVRPHVALITLIAPAHLGHFANLEEIAVAKAEIFEGIVPGGYALLNRDDKRFRQL
QELAEKAGVEHIATFGENARADYRLRDVKLHPTCSCMTVKIGTHEAAVKVGIPGRHVVQNMLAVLGAAHLVGADIAKVMM
AMATLSAQGGRGARHVLQHPEGAFTLIDESYNANPTSMRAALSLLHSTVPEGSRGRRIAVLGDMVELGRQSGKLHADLAR
PIVDAEVNTLFIGGSEMSVLKNALPVEIQTEYRQSTDELLPLVVKAVRPGDVVMVKSSNRTGFSKIVKALIAQFPPVEAA
E
>Mature_480_residues
SNRELSDWLWTSDAMVEAMEGRPFGNLPAGVTGISIDSRTLKPGEAFFAIKGEQFDGHDFATAAMAAGAGLLVVAEHRLP
AFGNTKVPMIVVEDVLEALIRLGIAARARSKAQIIAVTGSVGKTTTKEALRHVLSDVGKVHASAASFNNHWGVPLTLARM
PADVDYGVFEIGMNHHGEIRPLVKMVRPHVALITLIAPAHLGHFANLEEIAVAKAEIFEGIVPGGYALLNRDDKRFRQLQ
ELAEKAGVEHIATFGENARADYRLRDVKLHPTCSCMTVKIGTHEAAVKVGIPGRHVVQNMLAVLGAAHLVGADIAKVMMA
MATLSAQGGRGARHVLQHPEGAFTLIDESYNANPTSMRAALSLLHSTVPEGSRGRRIAVLGDMVELGRQSGKLHADLARP
IVDAEVNTLFIGGSEMSVLKNALPVEIQTEYRQSTDELLPLVVKAVRPGDVVMVKSSNRTGFSKIVKALIAQFPPVEAAE

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=437, Percent_Identity=33.4096109839817, Blast_Score=198, Evalue=8e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 51556; Mature: 51425

Theoretical pI: Translated: 7.62; Mature: 7.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNRELSDWLWTSDAMVEAMEGRPFGNLPAGVTGISIDSRTLKPGEAFFAIKGEQFDGHD
CCCCCCHHHHCCCHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCEEEEEECCCCCCCH
FATAAMAAGAGLLVVAEHRLPAFGNTKVPMIVVEDVLEALIRLGIAARARSKAQIIAVTG
HHHHHHHCCCCEEEEEECCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCEEEEEEEC
SVGKTTTKEALRHVLSDVGKVHASAASFNNHWGVPLTLARMPADVDYGVFEIGMNHHGEI
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCEEEECCCCCCCH
RPLVKMVRPHVALITLIAPAHLGHFANLEEIAVAKAEIFEGIVPGGYALLNRDDKRFRQL
HHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHH
QELAEKAGVEHIATFGENARADYRLRDVKLHPTCSCMTVKIGTHEAAVKVGIPGRHVVQN
HHHHHHCCHHHHHHCCCCCCCCEEEEEEEECCCCEEEEEEECCCCEEEEECCCHHHHHHH
MLAVLGAAHLVGADIAKVMMAMATLSAQGGRGARHVLQHPEGAFTLIDESYNANPTSMRA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCEEEEECCCCCCHHHHHH
ALSLLHSTVPEGSRGRRIAVLGDMVELGRQSGKLHADLARPIVDAEVNTLFIGGSEMSVL
HHHHHHHHCCCCCCCCEEEEEHHHHHHHHHCCCCHHHHHCCHHCCCCCEEEECCCHHHHH
KNALPVEIQTEYRQSTDELLPLVVKAVRPGDVVMVKSSNRTGFSKIVKALIAQFPPVEAA
HCCCCCEEEHHHHCCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCC
E
C
>Mature Secondary Structure 
SNRELSDWLWTSDAMVEAMEGRPFGNLPAGVTGISIDSRTLKPGEAFFAIKGEQFDGHD
CCCCCHHHHCCCHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCEEEEEECCCCCCCH
FATAAMAAGAGLLVVAEHRLPAFGNTKVPMIVVEDVLEALIRLGIAARARSKAQIIAVTG
HHHHHHHCCCCEEEEEECCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCEEEEEEEC
SVGKTTTKEALRHVLSDVGKVHASAASFNNHWGVPLTLARMPADVDYGVFEIGMNHHGEI
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCEEEECCCCCCCH
RPLVKMVRPHVALITLIAPAHLGHFANLEEIAVAKAEIFEGIVPGGYALLNRDDKRFRQL
HHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHH
QELAEKAGVEHIATFGENARADYRLRDVKLHPTCSCMTVKIGTHEAAVKVGIPGRHVVQN
HHHHHHCCHHHHHHCCCCCCCCEEEEEEEECCCCEEEEEEECCCCEEEEECCCHHHHHHH
MLAVLGAAHLVGADIAKVMMAMATLSAQGGRGARHVLQHPEGAFTLIDESYNANPTSMRA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCEEEEECCCCCCHHHHHH
ALSLLHSTVPEGSRGRRIAVLGDMVELGRQSGKLHADLARPIVDAEVNTLFIGGSEMSVL
HHHHHHHHCCCCCCCCEEEEEHHHHHHHHHCCCCHHHHHCCHHCCCCCEEEECCCHHHHH
KNALPVEIQTEYRQSTDELLPLVVKAVRPGDVVMVKSSNRTGFSKIVKALIAQFPPVEAA
HCCCCCEEEHHHHCCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]