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The map label for this gene is ddlB

Identifier: 225852910

GI number: 225852910

Start: 1404738

End: 1405664

Strand: Reverse

Name: ddlB

Synonym: BMEA_A1476

Alternate gene names: 225852910

Gene position: 1405664-1404738 (Counterclockwise)

Preceding gene: 225852911

Following gene: 225852909

Centisome position: 66.13

GC content: 55.56

Gene sequence:

>927_bases
ATGACGGGAAAACACGTCGCCGTTTTGATGGGTGGTTTTTCTTCGGAACGCTCCGTCAGCCTTTCCTCCGGTGTGGCCTG
TGCAACGACGCTTGAAGAATGCGGCTATCGCGTCACGCGCATTGATGTGGATCGCAATGTTGCGAGCATCCTGGTCGAGT
TGAAACCCGATGTTGTGTTCAACGCCTTGCACGGCCCCTTCGGCGAAGATGGCGCCATTCAGGGCGTGCTTGAATATCTC
CAGATTCCCTACACACATTCCGGCGTGCTGGCTTCCGCGCTTGCGATGGACAAGGATCGCGCCAAGAAGGTGGCGGCAGC
GGCGGGCGTCGTGGTTGCGCCGTCACGCCTGATGAACCGTTTCGACATTGGTTCGCAGCATCCGATGAAGCCGCCTTACG
TGGTGAAGCCGGTGCGTGAAGGGTCGAGCTTCGGTGTGGTGATCGTCAAGGAAGATCAGCCGCATCCGCCGCAGGTCATC
GGTTCGGCCGATTGGAAATATGGTGATGAGGTCATGGTCGAAGGCTATATTGCCGGGCGTGAGCTGACCTGTGCCGTGAT
GGGGGATCGCGCCATGGATGTGTGCGAGATCATGCCGGTCGGTTATCAGTTCTACGACTATGATTCAAAATATGTTGCAG
GTGCATCAACTCACGTGTGCCCGGCAAAAATTTTACCAAATATTTACCAAAAAATACAAACAATGGCCCTTACGGCGCAT
CGGGCAATCGGGTGTCGGGGCGTAAGCCGGTCGGACTTCCGTTTCGATGATCGTTTTTCTGAAGAAGGCGAAGTTGTCTG
GCTGGAAATCAATACCCAGCCGGGGATGACTCCTACCTCGCTCGTGCCTGATATCGCCAAAGCGGCGGGTATCTCTTTCG
CTGAGTTGTTGAGTTGGATGGTGGAGGACGCGTCTTGTTTGCGTTGA

Upstream 100 bases:

>100_bases
GGCAGGATTTTCCTGCTGTGGGTGCCTGCGGCTTGCGTCGCCGGGTTATCCGGACTCTGTTCTTGATTTATGCGCAGGAA
TGCGCAGAGGGGAAGCTAGA

Downstream 100 bases:

>100_bases
ACGGAAAATCGGATGGATATAGGCGCGCGGGCGCGGTGCGGGATGCATCGGGCGCGATGAATGCCGCTTTTGTCCTGCCG
CGCTTCCTGCGCAAGCCGTT

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase B; D-alanylalanine synthetase B

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MTGKHVAVLMGGFSSERSVSLSSGVACATTLEECGYRVTRIDVDRNVASILVELKPDVVFNALHGPFGEDGAIQGVLEYL
QIPYTHSGVLASALAMDKDRAKKVAAAAGVVVAPSRLMNRFDIGSQHPMKPPYVVKPVREGSSFGVVIVKEDQPHPPQVI
GSADWKYGDEVMVEGYIAGRELTCAVMGDRAMDVCEIMPVGYQFYDYDSKYVAGASTHVCPAKILPNIYQKIQTMALTAH
RAIGCRGVSRSDFRFDDRFSEEGEVVWLEINTQPGMTPTSLVPDIAKAAGISFAELLSWMVEDASCLR

Sequences:

>Translated_308_residues
MTGKHVAVLMGGFSSERSVSLSSGVACATTLEECGYRVTRIDVDRNVASILVELKPDVVFNALHGPFGEDGAIQGVLEYL
QIPYTHSGVLASALAMDKDRAKKVAAAAGVVVAPSRLMNRFDIGSQHPMKPPYVVKPVREGSSFGVVIVKEDQPHPPQVI
GSADWKYGDEVMVEGYIAGRELTCAVMGDRAMDVCEIMPVGYQFYDYDSKYVAGASTHVCPAKILPNIYQKIQTMALTAH
RAIGCRGVSRSDFRFDDRFSEEGEVVWLEINTQPGMTPTSLVPDIAKAAGISFAELLSWMVEDASCLR
>Mature_307_residues
TGKHVAVLMGGFSSERSVSLSSGVACATTLEECGYRVTRIDVDRNVASILVELKPDVVFNALHGPFGEDGAIQGVLEYLQ
IPYTHSGVLASALAMDKDRAKKVAAAAGVVVAPSRLMNRFDIGSQHPMKPPYVVKPVREGSSFGVVIVKEDQPHPPQVIG
SADWKYGDEVMVEGYIAGRELTCAVMGDRAMDVCEIMPVGYQFYDYDSKYVAGASTHVCPAKILPNIYQKIQTMALTAHR
AIGCRGVSRSDFRFDDRFSEEGEVVWLEINTQPGMTPTSLVPDIAKAAGISFAELLSWMVEDASCLR

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786280, Length=314, Percent_Identity=38.5350318471338, Blast_Score=191, Evalue=4e-50,
Organism=Escherichia coli, GI1786579, Length=256, Percent_Identity=36.71875, Blast_Score=152, Evalue=3e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDLB_BRUME (Q8YI63)

Other databases:

- EMBL:   AE008917
- PIR:   AH3324
- RefSeq:   NP_539499.1
- ProteinModelPortal:   Q8YI63
- SMR:   Q8YI63
- GeneID:   1196293
- GenomeReviews:   AE008917_GR
- KEGG:   bme:BMEI0582
- NMPDR:   fig|224914.1.peg.581
- HOGENOM:   HBG427092
- OMA:   PLSIVPE
- PhylomeDB:   Q8YI63
- ProtClustDB:   PRK01372
- BioCyc:   BMEL224914:BMEI0582-MONOMER
- BRENDA:   6.3.2.4
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 33413; Mature: 33281

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGKHVAVLMGGFSSERSVSLSSGVACATTLEECGYRVTRIDVDRNVASILVELKPDVVF
CCCCEEEEEEECCCCCCCEECCCCCHHHHHHHHHCCEEEEEECCCCHHHHEEECCCCCEE
NALHGPFGEDGAIQGVLEYLQIPYTHSGVLASALAMDKDRAKKVAAAAGVVVAPSRLMNR
EHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCHHHHHHHHHHCCEEECHHHHHHH
FDIGSQHPMKPPYVVKPVREGSSFGVVIVKEDQPHPPQVIGSADWKYGDEVMVEGYIAGR
HCCCCCCCCCCCEEEEECCCCCCEEEEEEECCCCCCCCEECCCCCCCCCEEEEEEEECCC
ELTCAVMGDRAMDVCEIMPVGYQFYDYDSKYVAGASTHVCPAKILPNIYQKIQTMALTAH
EEEEEEECCCHHHHHHHCCCCEEEEECCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHH
RAIGCRGVSRSDFRFDDRFSEEGEVVWLEINTQPGMTPTSLVPDIAKAAGISFAELLSWM
HHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHH
VEDASCLR
HHHHHHCC
>Mature Secondary Structure 
TGKHVAVLMGGFSSERSVSLSSGVACATTLEECGYRVTRIDVDRNVASILVELKPDVVF
CCCEEEEEEECCCCCCCEECCCCCHHHHHHHHHCCEEEEEECCCCHHHHEEECCCCCEE
NALHGPFGEDGAIQGVLEYLQIPYTHSGVLASALAMDKDRAKKVAAAAGVVVAPSRLMNR
EHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCHHHHHHHHHHCCEEECHHHHHHH
FDIGSQHPMKPPYVVKPVREGSSFGVVIVKEDQPHPPQVIGSADWKYGDEVMVEGYIAGR
HCCCCCCCCCCCEEEEECCCCCCEEEEEEECCCCCCCCEECCCCCCCCCEEEEEEEECCC
ELTCAVMGDRAMDVCEIMPVGYQFYDYDSKYVAGASTHVCPAKILPNIYQKIQTMALTAH
EEEEEEECCCHHHHHHHCCCCEEEEECCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHH
RAIGCRGVSRSDFRFDDRFSEEGEVVWLEINTQPGMTPTSLVPDIAKAAGISFAELLSWM
HHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHH
VEDASCLR
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11756688