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The map label for this gene is ligA [H]

Identifier: 225852902

GI number: 225852902

Start: 1393992

End: 1396151

Strand: Reverse

Name: ligA [H]

Synonym: BMEA_A1468

Alternate gene names: 225852902

Gene position: 1396151-1393992 (Counterclockwise)

Preceding gene: 225852903

Following gene: 225852901

Centisome position: 65.68

GC content: 59.95

Gene sequence:

>2160_bases
ATGTCCGATATTTCCGTCGAGAAACTGACCGAACTCGAAGCTGCCGCCGAACTGGAACGGCTGGCGCGCGCGATTGCTCA
TCACGACGAGCTTTATCACGCCAAGGACAGGCCGGAAATTTCCGATGCGGCTTATGATGCACTGAAGCGGCGCAACGAGG
CGATTGAAGCGCACTTTCCGGCACTTGTCCGCGATGACAGCCCGTCGCGGCGTGTGGGTGCAGCCCCGGCGCTTGCCACC
TTTGCGCCTGTTGTTCACGCGCGGCCCATGCTGTCGCTGGATAATGCCTTTAGTGATGAGGACGTGCGTGATTTTGTTGG
CAGCGTCTATCGCTTTCTGGGCCAACTGCCGGATGATTCCATCGCCTTTACCGCAGAACCGAAGATCGATGGGCTTTCCA
TGTCGATCCGTTACGAGAATGGAATTCTGGTTAGCGGTGCGACACGCGGGGATGGCACAACGGGCGAGAATGTAACAGCC
AATATCCGCACCATTGCTGAAATCCCGAACAGGCTTCCGGCAGGTGCGCCTGCTGTCGTGGAAGTGCGCGGCGAGGTCTA
TATGGCCAAGAGCGACTTTCTGACGCTCAATGCGCAGATGGCGGCGGAGGGCAAGCAGACCTATGTGAACCCGCGCAATA
CGGCAGCAGGTTCGCTGCGCCAGCTTGATGCGAAGGTGACGGCAAGCCGCAAGCTCAGGTTCTTCGCCTATGCCTGGGGG
GAAATGTCGGACATGCCTGCGGATACGCAATTGGGCATGGTCGAGGTTTTTCGCCAATGGGGCTTTCCGGTCAATCCGTT
GATGAAGCGGTTCAACAGCGTGGATGGGTTGCTTGCGCATTATCGCGCCATCGGCATGGAGCGTCCCACGCTGGATTACG
ATATCGACGGTGTTGTTTACAAGGTGGACCGGCTCGACCTGCAAACCCGGCTTGGTTTTCGCTCGCGCAGCCCGCGCTGG
GCGATTGCGCATAAATTCCCGGCGGAACAGGCGCTCACCATTTTGCGCGGCATCGATATTCAGGTGGGCCGCACTGGCGC
GCTGACGCCGGTGGCGCGGCTGGAGCCAATCACCGTCGGCGGTGTGGTTGTGACCAACGCGACGCTGCACAATGAGGACT
ATATCAAGGGCATAGGTCAGAAGGGCGAGCCGATCCGGGAGGGGCGCGATATCCGCATTGGCGATAGCGTGATCGTGCAG
CGCGCGGGCGATGTCATCCCGCAGATCGTGGATGTGGTGCTTGAGGAAGGCAAAAAACGCGGCGAGCCTTATCAGTTTCC
CCATGTCTGCCCCGCCTGCGGCAGCCATGCCGTGCGCGAGGAGGGCGAGGCGGTACGCCGCTGCACCGGCGGGCTGATCT
GCCCTGCGCAGGCGGTGGAACGCATCCGCCATTTTGTGTCGCGCAATGCCTTCGACATTGAGGGGCTTGGCGAAAAGCAG
GTCGAGTTCTTCTTCAATGCGGAAGATCCGGCCCTTTGCATCCGCTCGCCTGCCGATATTTTCACACTGAAAAAGCGGCA
GGAAAACTCGCTGACCAAGCTTCAGAATATCGAAGGCTTTGGCGCAACTTCCGTGAAGAAGCTTTATGACGCCATCGATG
CGCGGCGCGAGATTGCACTCCACCGCTTTCTGTTTGGTCTTGGCATCCGCCATGTGGGCGAGGTGAATGCCAAGCGCCTT
GCCCGCGCCTATCTTTCATATGCCGCTTTTGAGAAGGCGGCGCTGGAGGCTGTGCCGCCAAAAGAAGGCGACCGCACCGA
CAAGGGCAGCGAAGCGTGGCAGGACATGCTGGCCGTGGAGGGGATCGGTTCCATCGTGGCGGAAGCCGTGGTCGATTTTT
ATGGCGAGCCGCATAACCGCGAAGTGCTGGCCGCACTTCTTGCCGAAGTAACGCCGCTTGATGAGGAAGCGCGTGTGGCG
ACCGGTTCGCCCGTCGAGGGCAAGACGGTGGTTTTCACCGGCAGTCTGGAGCGCATGTCACGCGATGAAGCGAAGGCTAT
GGCTGAGCGCCATGGCGCGAAGACGGCAGGCTCTGTCTCCAAGAAGACCGATCTCGTGGTGGCAGGGCCGGGGGCCGGTT
CCAAGCTTGCCAAGGCGACGGAACTGGGCATTGAGGTTATCAATGAAGACGACTGGTTCAAGCTTGTTGGGGAGGATTGA

Upstream 100 bases:

>100_bases
GATAGTCAGGATTTGCTGCTTAGAGCAGTTCCTGTTTTAACGGAATCGCCGGAACCGCTCTGCGCAAAACCGTTTCACAC
TTTTGCTGGAAATGCTCTAG

Downstream 100 bases:

>100_bases
TGGCCGCATTCAATGGCTTCGGGGAGAAGGCCATTCCCTTTCTCAGGGCGCTCGATTTTCACCAGAACCGTGAATGGTTT
CTGGAAAACAGGGATTTGTT

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 719; Mature: 718

Protein sequence:

>719_residues
MSDISVEKLTELEAAAELERLARAIAHHDELYHAKDRPEISDAAYDALKRRNEAIEAHFPALVRDDSPSRRVGAAPALAT
FAPVVHARPMLSLDNAFSDEDVRDFVGSVYRFLGQLPDDSIAFTAEPKIDGLSMSIRYENGILVSGATRGDGTTGENVTA
NIRTIAEIPNRLPAGAPAVVEVRGEVYMAKSDFLTLNAQMAAEGKQTYVNPRNTAAGSLRQLDAKVTASRKLRFFAYAWG
EMSDMPADTQLGMVEVFRQWGFPVNPLMKRFNSVDGLLAHYRAIGMERPTLDYDIDGVVYKVDRLDLQTRLGFRSRSPRW
AIAHKFPAEQALTILRGIDIQVGRTGALTPVARLEPITVGGVVVTNATLHNEDYIKGIGQKGEPIREGRDIRIGDSVIVQ
RAGDVIPQIVDVVLEEGKKRGEPYQFPHVCPACGSHAVREEGEAVRRCTGGLICPAQAVERIRHFVSRNAFDIEGLGEKQ
VEFFFNAEDPALCIRSPADIFTLKKRQENSLTKLQNIEGFGATSVKKLYDAIDARREIALHRFLFGLGIRHVGEVNAKRL
ARAYLSYAAFEKAALEAVPPKEGDRTDKGSEAWQDMLAVEGIGSIVAEAVVDFYGEPHNREVLAALLAEVTPLDEEARVA
TGSPVEGKTVVFTGSLERMSRDEAKAMAERHGAKTAGSVSKKTDLVVAGPGAGSKLAKATELGIEVINEDDWFKLVGED

Sequences:

>Translated_719_residues
MSDISVEKLTELEAAAELERLARAIAHHDELYHAKDRPEISDAAYDALKRRNEAIEAHFPALVRDDSPSRRVGAAPALAT
FAPVVHARPMLSLDNAFSDEDVRDFVGSVYRFLGQLPDDSIAFTAEPKIDGLSMSIRYENGILVSGATRGDGTTGENVTA
NIRTIAEIPNRLPAGAPAVVEVRGEVYMAKSDFLTLNAQMAAEGKQTYVNPRNTAAGSLRQLDAKVTASRKLRFFAYAWG
EMSDMPADTQLGMVEVFRQWGFPVNPLMKRFNSVDGLLAHYRAIGMERPTLDYDIDGVVYKVDRLDLQTRLGFRSRSPRW
AIAHKFPAEQALTILRGIDIQVGRTGALTPVARLEPITVGGVVVTNATLHNEDYIKGIGQKGEPIREGRDIRIGDSVIVQ
RAGDVIPQIVDVVLEEGKKRGEPYQFPHVCPACGSHAVREEGEAVRRCTGGLICPAQAVERIRHFVSRNAFDIEGLGEKQ
VEFFFNAEDPALCIRSPADIFTLKKRQENSLTKLQNIEGFGATSVKKLYDAIDARREIALHRFLFGLGIRHVGEVNAKRL
ARAYLSYAAFEKAALEAVPPKEGDRTDKGSEAWQDMLAVEGIGSIVAEAVVDFYGEPHNREVLAALLAEVTPLDEEARVA
TGSPVEGKTVVFTGSLERMSRDEAKAMAERHGAKTAGSVSKKTDLVVAGPGAGSKLAKATELGIEVINEDDWFKLVGED
>Mature_718_residues
SDISVEKLTELEAAAELERLARAIAHHDELYHAKDRPEISDAAYDALKRRNEAIEAHFPALVRDDSPSRRVGAAPALATF
APVVHARPMLSLDNAFSDEDVRDFVGSVYRFLGQLPDDSIAFTAEPKIDGLSMSIRYENGILVSGATRGDGTTGENVTAN
IRTIAEIPNRLPAGAPAVVEVRGEVYMAKSDFLTLNAQMAAEGKQTYVNPRNTAAGSLRQLDAKVTASRKLRFFAYAWGE
MSDMPADTQLGMVEVFRQWGFPVNPLMKRFNSVDGLLAHYRAIGMERPTLDYDIDGVVYKVDRLDLQTRLGFRSRSPRWA
IAHKFPAEQALTILRGIDIQVGRTGALTPVARLEPITVGGVVVTNATLHNEDYIKGIGQKGEPIREGRDIRIGDSVIVQR
AGDVIPQIVDVVLEEGKKRGEPYQFPHVCPACGSHAVREEGEAVRRCTGGLICPAQAVERIRHFVSRNAFDIEGLGEKQV
EFFFNAEDPALCIRSPADIFTLKKRQENSLTKLQNIEGFGATSVKKLYDAIDARREIALHRFLFGLGIRHVGEVNAKRLA
RAYLSYAAFEKAALEAVPPKEGDRTDKGSEAWQDMLAVEGIGSIVAEAVVDFYGEPHNREVLAALLAEVTPLDEEARVAT
GSPVEGKTVVFTGSLERMSRDEAKAMAERHGAKTAGSVSKKTDLVVAGPGAGSKLAKATELGIEVINEDDWFKLVGED

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Escherichia coli, GI1788750, Length=708, Percent_Identity=46.7514124293785, Blast_Score=590, Evalue=1e-170,
Organism=Escherichia coli, GI87082305, Length=552, Percent_Identity=21.7391304347826, Blast_Score=89, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 78624; Mature: 78493

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS50172 BRCT ; PS01055 DNA_LIGASE_N1 ; PS01056 DNA_LIGASE_N2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDISVEKLTELEAAAELERLARAIAHHDELYHAKDRPEISDAAYDALKRRNEAIEAHFP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCC
ALVRDDSPSRRVGAAPALATFAPVVHARPMLSLDNAFSDEDVRDFVGSVYRFLGQLPDDS
EEEECCCCCCCCCCCHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHHHCCCCCC
IAFTAEPKIDGLSMSIRYENGILVSGATRGDGTTGENVTANIRTIAEIPNRLPAGAPAVV
EEEEECCCCCCEEEEEEECCCEEEECCCCCCCCCCCCCEEHHHHHHHHHHCCCCCCCEEE
EVRGEVYMAKSDFLTLNAQMAAEGKQTYVNPRNTAAGSLRQLDAKVTASRKLRFFAYAWG
EECCCEEEEECCEEEEEHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHCCCEEEEEEECC
EMSDMPADTQLGMVEVFRQWGFPVNPLMKRFNSVDGLLAHYRAIGMERPTLDYDIDGVVY
CCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEE
KVDRLDLQTRLGFRSRSPRWAIAHKFPAEQALTILRGIDIQVGRTGALTPVARLEPITVG
EEEHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHCCCEEECCCCCCCCHHHCCCEEEC
GVVVTNATLHNEDYIKGIGQKGEPIREGRDIRIGDSVIVQRAGDVIPQIVDVVLEEGKKR
CEEEEEEEECCCHHHHCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHC
GEPYQFPHVCPACGSHAVREEGEAVRRCTGGLICPAQAVERIRHFVSRNAFDIEGLGEKQ
CCCCCCCCCCCHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCE
VEFFFNAEDPALCIRSPADIFTLKKRQENSLTKLQNIEGFGATSVKKLYDAIDARREIAL
EEEEECCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
HRFLFGLGIRHVGEVNAKRLARAYLSYAAFEKAALEAVPPKEGDRTDKGSEAWQDMLAVE
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
GIGSIVAEAVVDFYGEPHNREVLAALLAEVTPLDEEARVATGSPVEGKTVVFTGSLERMS
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCHHHHH
RDEAKAMAERHGAKTAGSVSKKTDLVVAGPGAGSKLAKATELGIEVINEDDWFKLVGED
HHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHCCEEECCCCEEEECCCC
>Mature Secondary Structure 
SDISVEKLTELEAAAELERLARAIAHHDELYHAKDRPEISDAAYDALKRRNEAIEAHFP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCC
ALVRDDSPSRRVGAAPALATFAPVVHARPMLSLDNAFSDEDVRDFVGSVYRFLGQLPDDS
EEEECCCCCCCCCCCHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHHHCCCCCC
IAFTAEPKIDGLSMSIRYENGILVSGATRGDGTTGENVTANIRTIAEIPNRLPAGAPAVV
EEEEECCCCCCEEEEEEECCCEEEECCCCCCCCCCCCCEEHHHHHHHHHHCCCCCCCEEE
EVRGEVYMAKSDFLTLNAQMAAEGKQTYVNPRNTAAGSLRQLDAKVTASRKLRFFAYAWG
EECCCEEEEECCEEEEEHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHCCCEEEEEEECC
EMSDMPADTQLGMVEVFRQWGFPVNPLMKRFNSVDGLLAHYRAIGMERPTLDYDIDGVVY
CCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEE
KVDRLDLQTRLGFRSRSPRWAIAHKFPAEQALTILRGIDIQVGRTGALTPVARLEPITVG
EEEHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHCCCEEECCCCCCCCHHHCCCEEEC
GVVVTNATLHNEDYIKGIGQKGEPIREGRDIRIGDSVIVQRAGDVIPQIVDVVLEEGKKR
CEEEEEEEECCCHHHHCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHC
GEPYQFPHVCPACGSHAVREEGEAVRRCTGGLICPAQAVERIRHFVSRNAFDIEGLGEKQ
CCCCCCCCCCCHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCE
VEFFFNAEDPALCIRSPADIFTLKKRQENSLTKLQNIEGFGATSVKKLYDAIDARREIAL
EEEEECCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
HRFLFGLGIRHVGEVNAKRLARAYLSYAAFEKAALEAVPPKEGDRTDKGSEAWQDMLAVE
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
GIGSIVAEAVVDFYGEPHNREVLAALLAEVTPLDEEARVATGSPVEGKTVVFTGSLERMS
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCHHHHH
RDEAKAMAERHGAKTAGSVSKKTDLVVAGPGAGSKLAKATELGIEVINEDDWFKLVGED
HHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHCCEEECCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA