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The map label for this gene is kefA [C]

Identifier: 225852810

GI number: 225852810

Start: 1301272

End: 1303854

Strand: Reverse

Name: kefA [C]

Synonym: BMEA_A1368

Alternate gene names: 225852810

Gene position: 1303854-1301272 (Counterclockwise)

Preceding gene: 225852811

Following gene: 225852809

Centisome position: 61.34

GC content: 57.22

Gene sequence:

>2583_bases
TTGAAATTGATCCCTTTATCGACATTCCGCGTCCTTGCTGTTTCTCTGCTGGCTGGCGCTCTCTCGCTGGGTGCGCTTGC
GCCGGCCGTGGCGCAGAATGACAAGCAGCAAAACCCGCCTGCCTCGCAGTCCGCGCCGGCGGAAAAGCCGAATGGGCAGG
CAGCTTCCGCTGCCGATCAACGGCCCCCCGAACCCGTATCGAGCCCCGTATCAAGCATTGTACAGCAGCAGCGGCCCACG
TTCGATGAGCTGAAGCAACTGACGAAGAAGATCAACGAGCAGCTTTCCAAGTCCGCGTCGAGCGATGAGGCGCTTGCCAA
TCTCAAGCTTCAGCTTGACGCCCTTTCCAAGAAGCTTCTGGAAACGGGCGTTGCCTTCCGCCCGCGCCTTACCGAGATCA
ACACGCGGCTGGAGCAGCTTGGCCCCGCGCCTTCGAGCGATCAGCCGGCGGAGCCGGCCATCGTTGGGGAAGAGCGTGCA
CGGCTGATAGCGGAAAAGGCAGAAATCAACGCTACCCTTGGCGAAACGGAAGACACGTCGCTTGCCGTGAACCGCATGTC
TGCGTTGATCGGCGATATGCGGCGTGATCTCTTCACCAAGACGCTTTCGCAGCGCGTCAATCTGGATTCGACGCTTGGCA
GCGAAGTGGTTTCGGCTGCAAGCGACCAGATGATATCGTTGTGGCGTATCGTGCGATCGTGGTGGCGTTTCGTTGTCACC
TTCAAGCTTGAATCGTTTCTGGCTGCCGCTTTCTTTGCCCTTGCCGCCGCATTGGTGCTTCAGTTCGGCGCGCAGCGGTT
TCTGGGCGCCTTTTACCGGCGCGACCCGTCCGTCGAATCCCCATCCTATCTGAGCCGTTTATCGGTTGCGTTCTGGTCAA
CGGTCATTCCGTCAGCAGCCGTTGGCGTCTTTCTGGCGACGACATATTTCCTCCTCAATTACTTCAATGTGTTGAGGACG
GATATTGCATCGCTTTTCCAATCGCTTTTCATCGTATTGGGGCTGGTGTTCTTCATTCACCGGCTGGCCGTGGCCTGCAT
CAGTTCCGATATGCCGCAATGGCGGCTGGTGCAGGTGGCCCCGCGCCCGGGGCATCTTCTGGCCTGGCTGGTGACAGCCA
CCGCACTTACCAGCGGACTTGATTCCTTCTTTGGAACAGTCAATCGCATACTTTCCTCGCCACTCTCGCTGACTATGGCA
AAAAGCCTGATCGCGACGGTGATTATCGGGGTGCTCATTCTGGCCATCGCCTTTGTCAAGCCTGTCGAGCGAGAGAAGGA
TGGCGCTGTCCGTGCGTGGCCACGCGCTTTCAGGATATTCCTGATTTTGATGGGGCTCTTGCCTATTCTCACGGCCCTTT
CCGGCTATATCGGCATTGCGCGTTTCATCTCGCAGCAGATCGTCGTGACGGGCGCTTTCCTTGTCACCATGTATATGGGC
TTCCTGACAGGGCGCGCGATTTCCGAGGAGCAGGCCTTCGCCTCAAGCCGGATCGGCAAGGCAATGCGCGAGCGTTTTCA
TTTCGATGAAGCAACGCTCGACCAGCTTGGCCTTCTGGCTGGTATTCTCATCAATCTGGTCGTCGCGCTGATCGGCATTC
CGCTGGTTTTGATGCAGCTTGGTTTTCAGTGGGCGGAGCTGAAAAGCACATTCTACAAGCTGATGACCGGCTTCCAGATC
GGAAATTTCTCCATCTCGCTCATGGGGCTCCTGTCGGGTGTGCTGCTGTTTCTCATCGGCTATGTCCTGACGCGCTGGTT
CCAGAACTGGCTGGATAACAGCGTCATGGCGCGTGGCCGGGTGGATTCGGGTGTGCGCAATTCCATCCGCACTGTTGTCG
GTTATGTCGGGCTTTGTCTTGCGGCGCTGATGGGCATTTCGGCGGCCGGGTTCAACCTTGCCAATCTCGCACTGATTGCT
GGCGGCCTCTCTCTCGGTATCGGTTTCGGCCTCCAGAATATCGTCCAGAACTTTGTTTCCGGCCTGATCCTGCTGGCAGA
ACGCCCCTTCAAGGTGGGCGACTGGGTGGAGGCGGGTACGGTCAGCGGCATTGTGAAGAAGATCAGCGTGCGCGCGACGG
AAGTGGAAACATTCCAGAAACAGTCGATTATCGTGCCGAATTCGACGCTCATCAACGGCAATGTCGGCAACTGGACGCAC
CGCAACAAGCTTGGCCGCATCGACATCAATGTGCAGGCTTCCTTTACAGAAGACCCGCGCCGCGTCCACGCGCTTTTGCT
GGAGATCGTGCGTGGCCATCCATCCATTCTGAAGAACCCGGAACCGTTCGTTTCCTTTCAGAGCATGACCGGTTCGCTGC
TCGTTTTCGATGTTTATGCCCATGTGGCCGACATTACGTCGACCGGCAGTATCAAGAACGAATTGCGATTCCAGATTGTC
GAGCGTTTCCATGAACAGGGGTTGAGCCTGTCATCTTCCTCGACAGACCTTATATTGAAGGCCCCCGATGTGGAGAAACT
TTCGGAACTGATGCAGGAGGAAAAAGGACTTTCAGCGGCAGCCAGCAGGGAGAAGACGGGCGAAAAGAAGCCTGAGGAGG
GTGACAAGGACGATCGTGCGTAA

Upstream 100 bases:

>100_bases
GTTGCTTCACGAATAAAGCGAAATCTTGTAAGTGGATGGCTCCTTGTTTCCATTCTAACGCGCTTTATGTTGAAGCGGTG
CTCTTACTTTAACGGCAGAA

Downstream 100 bases:

>100_bases
CCGGATTTCGTTCAGTTGCGGTTCAGTTTTGTCAGCCTATAAGAAATTCGCAAATGCGGGAAGCCTCACGAAAGCGGGGC
CAGAGTGATGGAAAGGTCGG

Product: mechanosensitive ion channel protein MscS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 860; Mature: 860

Protein sequence:

>860_residues
MKLIPLSTFRVLAVSLLAGALSLGALAPAVAQNDKQQNPPASQSAPAEKPNGQAASAADQRPPEPVSSPVSSIVQQQRPT
FDELKQLTKKINEQLSKSASSDEALANLKLQLDALSKKLLETGVAFRPRLTEINTRLEQLGPAPSSDQPAEPAIVGEERA
RLIAEKAEINATLGETEDTSLAVNRMSALIGDMRRDLFTKTLSQRVNLDSTLGSEVVSAASDQMISLWRIVRSWWRFVVT
FKLESFLAAAFFALAAALVLQFGAQRFLGAFYRRDPSVESPSYLSRLSVAFWSTVIPSAAVGVFLATTYFLLNYFNVLRT
DIASLFQSLFIVLGLVFFIHRLAVACISSDMPQWRLVQVAPRPGHLLAWLVTATALTSGLDSFFGTVNRILSSPLSLTMA
KSLIATVIIGVLILAIAFVKPVEREKDGAVRAWPRAFRIFLILMGLLPILTALSGYIGIARFISQQIVVTGAFLVTMYMG
FLTGRAISEEQAFASSRIGKAMRERFHFDEATLDQLGLLAGILINLVVALIGIPLVLMQLGFQWAELKSTFYKLMTGFQI
GNFSISLMGLLSGVLLFLIGYVLTRWFQNWLDNSVMARGRVDSGVRNSIRTVVGYVGLCLAALMGISAAGFNLANLALIA
GGLSLGIGFGLQNIVQNFVSGLILLAERPFKVGDWVEAGTVSGIVKKISVRATEVETFQKQSIIVPNSTLINGNVGNWTH
RNKLGRIDINVQASFTEDPRRVHALLLEIVRGHPSILKNPEPFVSFQSMTGSLLVFDVYAHVADITSTGSIKNELRFQIV
ERFHEQGLSLSSSSTDLILKAPDVEKLSELMQEEKGLSAAASREKTGEKKPEEGDKDDRA

Sequences:

>Translated_860_residues
MKLIPLSTFRVLAVSLLAGALSLGALAPAVAQNDKQQNPPASQSAPAEKPNGQAASAADQRPPEPVSSPVSSIVQQQRPT
FDELKQLTKKINEQLSKSASSDEALANLKLQLDALSKKLLETGVAFRPRLTEINTRLEQLGPAPSSDQPAEPAIVGEERA
RLIAEKAEINATLGETEDTSLAVNRMSALIGDMRRDLFTKTLSQRVNLDSTLGSEVVSAASDQMISLWRIVRSWWRFVVT
FKLESFLAAAFFALAAALVLQFGAQRFLGAFYRRDPSVESPSYLSRLSVAFWSTVIPSAAVGVFLATTYFLLNYFNVLRT
DIASLFQSLFIVLGLVFFIHRLAVACISSDMPQWRLVQVAPRPGHLLAWLVTATALTSGLDSFFGTVNRILSSPLSLTMA
KSLIATVIIGVLILAIAFVKPVEREKDGAVRAWPRAFRIFLILMGLLPILTALSGYIGIARFISQQIVVTGAFLVTMYMG
FLTGRAISEEQAFASSRIGKAMRERFHFDEATLDQLGLLAGILINLVVALIGIPLVLMQLGFQWAELKSTFYKLMTGFQI
GNFSISLMGLLSGVLLFLIGYVLTRWFQNWLDNSVMARGRVDSGVRNSIRTVVGYVGLCLAALMGISAAGFNLANLALIA
GGLSLGIGFGLQNIVQNFVSGLILLAERPFKVGDWVEAGTVSGIVKKISVRATEVETFQKQSIIVPNSTLINGNVGNWTH
RNKLGRIDINVQASFTEDPRRVHALLLEIVRGHPSILKNPEPFVSFQSMTGSLLVFDVYAHVADITSTGSIKNELRFQIV
ERFHEQGLSLSSSSTDLILKAPDVEKLSELMQEEKGLSAAASREKTGEKKPEEGDKDDRA
>Mature_860_residues
MKLIPLSTFRVLAVSLLAGALSLGALAPAVAQNDKQQNPPASQSAPAEKPNGQAASAADQRPPEPVSSPVSSIVQQQRPT
FDELKQLTKKINEQLSKSASSDEALANLKLQLDALSKKLLETGVAFRPRLTEINTRLEQLGPAPSSDQPAEPAIVGEERA
RLIAEKAEINATLGETEDTSLAVNRMSALIGDMRRDLFTKTLSQRVNLDSTLGSEVVSAASDQMISLWRIVRSWWRFVVT
FKLESFLAAAFFALAAALVLQFGAQRFLGAFYRRDPSVESPSYLSRLSVAFWSTVIPSAAVGVFLATTYFLLNYFNVLRT
DIASLFQSLFIVLGLVFFIHRLAVACISSDMPQWRLVQVAPRPGHLLAWLVTATALTSGLDSFFGTVNRILSSPLSLTMA
KSLIATVIIGVLILAIAFVKPVEREKDGAVRAWPRAFRIFLILMGLLPILTALSGYIGIARFISQQIVVTGAFLVTMYMG
FLTGRAISEEQAFASSRIGKAMRERFHFDEATLDQLGLLAGILINLVVALIGIPLVLMQLGFQWAELKSTFYKLMTGFQI
GNFSISLMGLLSGVLLFLIGYVLTRWFQNWLDNSVMARGRVDSGVRNSIRTVVGYVGLCLAALMGISAAGFNLANLALIA
GGLSLGIGFGLQNIVQNFVSGLILLAERPFKVGDWVEAGTVSGIVKKISVRATEVETFQKQSIIVPNSTLINGNVGNWTH
RNKLGRIDINVQASFTEDPRRVHALLLEIVRGHPSILKNPEPFVSFQSMTGSLLVFDVYAHVADITSTGSIKNELRFQIV
ERFHEQGLSLSSSSTDLILKAPDVEKLSELMQEEKGLSAAASREKTGEKKPEEGDKDDRA

Specific function: Unknown

COG id: COG3264

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786670, Length=273, Percent_Identity=27.8388278388278, Blast_Score=139, Evalue=8e-34,
Organism=Escherichia coli, GI2367355, Length=346, Percent_Identity=26.878612716763, Blast_Score=130, Evalue=5e-31,
Organism=Escherichia coli, GI1789291, Length=263, Percent_Identity=22.8136882129278, Blast_Score=88, Evalue=3e-18,
Organism=Escherichia coli, GI1787591, Length=239, Percent_Identity=26.7782426778243, Blast_Score=70, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 93701; Mature: 93701

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: PS01246 UPF0003

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIPLSTFRVLAVSLLAGALSLGALAPAVAQNDKQQNPPASQSAPAEKPNGQAASAADQ
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
RPPEPVSSPVSSIVQQQRPTFDELKQLTKKINEQLSKSASSDEALANLKLQLDALSKKLL
CCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
ETGVAFRPRLTEINTRLEQLGPAPSSDQPAEPAIVGEERARLIAEKAEINATLGETEDTS
HCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEECHHHHHHHHHHHHHCCCCCCCCCHH
LAVNRMSALIGDMRRDLFTKTLSQRVNLDSTLGSEVVSAASDQMISLWRIVRSWWRFVVT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FKLESFLAAAFFALAAALVLQFGAQRFLGAFYRRDPSVESPSYLSRLSVAFWSTVIPSAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
VGVFLATTYFLLNYFNVLRTDIASLFQSLFIVLGLVFFIHRLAVACISSDMPQWRLVQVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
PRPGHLLAWLVTATALTSGLDSFFGTVNRILSSPLSLTMAKSLIATVIIGVLILAIAFVK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
PVEREKDGAVRAWPRAFRIFLILMGLLPILTALSGYIGIARFISQQIVVTGAFLVTMYMG
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLTGRAISEEQAFASSRIGKAMRERFHFDEATLDQLGLLAGILINLVVALIGIPLVLMQL
HHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GFQWAELKSTFYKLMTGFQIGNFSISLMGLLSGVLLFLIGYVLTRWFQNWLDNSVMARGR
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCC
VDSGVRNSIRTVVGYVGLCLAALMGISAAGFNLANLALIAGGLSLGIGFGLQNIVQNFVS
CCHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLILLAERPFKVGDWVEAGTVSGIVKKISVRATEVETFQKQSIIVPNSTLINGNVGNWTH
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCEEECCCCCCCHH
RNKLGRIDINVQASFTEDPRRVHALLLEIVRGHPSILKNPEPFVSFQSMTGSLLVFDVYA
HCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCHHHCCCCCCCHHHHHCCCCHHHHHHHH
HVADITSTGSIKNELRFQIVERFHEQGLSLSSSSTDLILKAPDVEKLSELMQEEKGLSAA
HHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHHHCCCHHH
ASREKTGEKKPEEGDKDDRA
HHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKLIPLSTFRVLAVSLLAGALSLGALAPAVAQNDKQQNPPASQSAPAEKPNGQAASAADQ
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
RPPEPVSSPVSSIVQQQRPTFDELKQLTKKINEQLSKSASSDEALANLKLQLDALSKKLL
CCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
ETGVAFRPRLTEINTRLEQLGPAPSSDQPAEPAIVGEERARLIAEKAEINATLGETEDTS
HCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEECHHHHHHHHHHHHHCCCCCCCCCHH
LAVNRMSALIGDMRRDLFTKTLSQRVNLDSTLGSEVVSAASDQMISLWRIVRSWWRFVVT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FKLESFLAAAFFALAAALVLQFGAQRFLGAFYRRDPSVESPSYLSRLSVAFWSTVIPSAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
VGVFLATTYFLLNYFNVLRTDIASLFQSLFIVLGLVFFIHRLAVACISSDMPQWRLVQVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
PRPGHLLAWLVTATALTSGLDSFFGTVNRILSSPLSLTMAKSLIATVIIGVLILAIAFVK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
PVEREKDGAVRAWPRAFRIFLILMGLLPILTALSGYIGIARFISQQIVVTGAFLVTMYMG
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLTGRAISEEQAFASSRIGKAMRERFHFDEATLDQLGLLAGILINLVVALIGIPLVLMQL
HHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GFQWAELKSTFYKLMTGFQIGNFSISLMGLLSGVLLFLIGYVLTRWFQNWLDNSVMARGR
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCC
VDSGVRNSIRTVVGYVGLCLAALMGISAAGFNLANLALIAGGLSLGIGFGLQNIVQNFVS
CCHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLILLAERPFKVGDWVEAGTVSGIVKKISVRATEVETFQKQSIIVPNSTLINGNVGNWTH
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCEEECCCCCCCHH
RNKLGRIDINVQASFTEDPRRVHALLLEIVRGHPSILKNPEPFVSFQSMTGSLLVFDVYA
HCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCHHHCCCCCCCHHHHHCCCCHHHHHHHH
HVADITSTGSIKNELRFQIVERFHEQGLSLSSSSTDLILKAPDVEKLSELMQEEKGLSAA
HHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHHHCCCHHH
ASREKTGEKKPEEGDKDDRA
HHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]