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The map label for this gene is cobD

Identifier: 225852785

GI number: 225852785

Start: 1274658

End: 1275641

Strand: Reverse

Name: cobD

Synonym: BMEA_A1341

Alternate gene names: 225852785

Gene position: 1275641-1274658 (Counterclockwise)

Preceding gene: 225852786

Following gene: 225852783

Centisome position: 60.01

GC content: 59.04

Gene sequence:

>984_bases
ATGGAAATAAAACTTATTGTCCTTTCGCTGGCGCTCCTTCTCGACCGCTTCATCGGTGATCTACCGCTGCTTTGGCAAAG
GATTTCGCATCCGGTCGTGCTCTTGGGCAAGGCGATTTCCTGGGGTGAAAAAAATTTCAACGACAGCAGCCTGCCGCCTT
CAACGTTGCGCCGCAATGGCATGTGGCTGACGGTTGGGCTGGTGGCGGCTTGTATTTTCGCCGGATTGGTGATCCGGAGT
ATTTTGCCCCATGCCGGTACGGCGGGTGCTATTGCCGAAGTCGTAATTGTTGCCATTCTGCTTGCGCAAAAAAGCCTTGC
CGATCATGTGCAGGCCGTGGCGCAAGCCTTGCGTGACGATGGCATAGAAGGTGGCCGCAAGGCGGTCTCCATGATCGTGG
GGCGAAACCCCGACCGACTGGATGAAGGCGGCGTCAGCCGTGCTGCCATTGAAAGCCTTGCGGAAAATGCATCCGACGGG
ATCGTTGCGCCGGCATTCTGGTTTCTCGTCGGCGGATTGCCGGGGCTGTTTGCCTATAAGTTTATCAATACGGCCGATTC
CATGATCGGGCATCTGAATGATCGCTATCGCGATTTCGGCCGGTTCGCTGCAAAACTCGATGATGTCGCCAATTATATTC
CTGCCCGGCTGACAGGTCTGCTCGCCGCACTTGCAACCTCCCTCGGCCATGGCAGGGAAGCGGGGAGGCAGGCGCTCTCG
ATCATGTGCCGGGATGCTAGGCTGCATCGATCGCCCAATGCGGGCTGGCCGGAAGCGGCATTTGCAGGGGCGCTGGGGCT
GGCGCTTGCCGGTCCGCGCCAATATGGCGCCGAGACGGTTGAGGGGCCGATGCTCAACGCCACTGGAAAACGCGAGGCCG
AGGCGAGGGATATCGATGCCGCGCTGGTGCTGTTCTGGTCCACGATGAGCCTGATGACCGGACTGGTGATAGCGGCGAGC
CTTGTCGGCCTATTTGTCGGTTAG

Upstream 100 bases:

>100_bases
TATCGGCCTTGCGCATGACGACGCTGCCCTGGCGCGGCTGGAACAAGCATTGTGCGACATGTGAAGTTGAAATTTTGATT
CAGGTTTTTTGGCCAACTCC

Downstream 100 bases:

>100_bases
GATGAACCGTTTGCCGTCGAAGCGCTGCAAACGGTTGGGATTGTCCGTCCTGATGCCGTTTTCATCAAATTTGATGGTGC
CCAGAGCGGTTTCAAAGGAG

Product: cobalamin biosynthesis protein

Products: ADP; phosphate; Adenosyl cobinamide phosphate; Adenosyl cobinamide

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MEIKLIVLSLALLLDRFIGDLPLLWQRISHPVVLLGKAISWGEKNFNDSSLPPSTLRRNGMWLTVGLVAACIFAGLVIRS
ILPHAGTAGAIAEVVIVAILLAQKSLADHVQAVAQALRDDGIEGGRKAVSMIVGRNPDRLDEGGVSRAAIESLAENASDG
IVAPAFWFLVGGLPGLFAYKFINTADSMIGHLNDRYRDFGRFAAKLDDVANYIPARLTGLLAALATSLGHGREAGRQALS
IMCRDARLHRSPNAGWPEAAFAGALGLALAGPRQYGAETVEGPMLNATGKREAEARDIDAALVLFWSTMSLMTGLVIAAS
LVGLFVG

Sequences:

>Translated_327_residues
MEIKLIVLSLALLLDRFIGDLPLLWQRISHPVVLLGKAISWGEKNFNDSSLPPSTLRRNGMWLTVGLVAACIFAGLVIRS
ILPHAGTAGAIAEVVIVAILLAQKSLADHVQAVAQALRDDGIEGGRKAVSMIVGRNPDRLDEGGVSRAAIESLAENASDG
IVAPAFWFLVGGLPGLFAYKFINTADSMIGHLNDRYRDFGRFAAKLDDVANYIPARLTGLLAALATSLGHGREAGRQALS
IMCRDARLHRSPNAGWPEAAFAGALGLALAGPRQYGAETVEGPMLNATGKREAEARDIDAALVLFWSTMSLMTGLVIAAS
LVGLFVG
>Mature_327_residues
MEIKLIVLSLALLLDRFIGDLPLLWQRISHPVVLLGKAISWGEKNFNDSSLPPSTLRRNGMWLTVGLVAACIFAGLVIRS
ILPHAGTAGAIAEVVIVAILLAQKSLADHVQAVAQALRDDGIEGGRKAVSMIVGRNPDRLDEGGVSRAAIESLAENASDG
IVAPAFWFLVGGLPGLFAYKFINTADSMIGHLNDRYRDFGRFAAKLDDVANYIPARLTGLLAALATSLGHGREAGRQALS
IMCRDARLHRSPNAGWPEAAFAGALGLALAGPRQYGAETVEGPMLNATGKREAEARDIDAALVLFWSTMSLMTGLVIAAS
LVGLFVG

Specific function: Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group

COG id: COG1270

COG function: function code H; Cobalamin biosynthesis protein CobD/CbiB

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobD/CbiB family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBD_BRUME (Q8YHT9)

Other databases:

- EMBL:   AE008917
- PIR:   AE3340
- RefSeq:   NP_539624.1
- GeneID:   1196418
- GenomeReviews:   AE008917_GR
- KEGG:   bme:BMEI0707
- NMPDR:   fig|224914.1.peg.706
- HOGENOM:   HBG647131
- OMA:   IGDPWGW
- ProtClustDB:   PRK01209
- BioCyc:   BMEL224914:BMEI0707-MONOMER
- HAMAP:   MF_00024
- InterPro:   IPR004485
- TIGRFAMs:   TIGR00380

Pfam domain/function: PF03186 CobD_Cbib

EC number: 6.3.1.10

Molecular weight: Translated: 34640; Mature: 34640

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1f81c37c)-; HASH(0x228332e0)-; HASH(0x21fb8f14)-; HASH(0x22442e5c)-;

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIKLIVLSLALLLDRFIGDLPLLWQRISHPVVLLGKAISWGEKNFNDSSLPPSTLRRNG
CCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEHHHHHHCCCCCCCCCCCCHHHHHHCC
MWLTVGLVAACIFAGLVIRSILPHAGTAGAIAEVVIVAILLAQKSLADHVQAVAQALRDD
CEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GIEGGRKAVSMIVGRNPDRLDEGGVSRAAIESLAENASDGIVAPAFWFLVGGLPGLFAYK
CCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHH
FINTADSMIGHLNDRYRDFGRFAAKLDDVANYIPARLTGLLAALATSLGHGREAGRQALS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
IMCRDARLHRSPNAGWPEAAFAGALGLALAGPRQYGAETVEGPMLNATGKREAEARDIDA
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHCCHHHCCCCCCCCCCCCHHHHHHHH
ALVLFWSTMSLMTGLVIAASLVGLFVG
HHHHHHHHHHHHHHHHHHHHHHHHHCH
>Mature Secondary Structure
MEIKLIVLSLALLLDRFIGDLPLLWQRISHPVVLLGKAISWGEKNFNDSSLPPSTLRRNG
CCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEHHHHHHCCCCCCCCCCCCHHHHHHCC
MWLTVGLVAACIFAGLVIRSILPHAGTAGAIAEVVIVAILLAQKSLADHVQAVAQALRDD
CEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GIEGGRKAVSMIVGRNPDRLDEGGVSRAAIESLAENASDGIVAPAFWFLVGGLPGLFAYK
CCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHH
FINTADSMIGHLNDRYRDFGRFAAKLDDVANYIPARLTGLLAALATSLGHGREAGRQALS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
IMCRDARLHRSPNAGWPEAAFAGALGLALAGPRQYGAETVEGPMLNATGKREAEARDIDA
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHCCHHHCCCCCCCCCCCCHHHHHHHH
ALVLFWSTMSLMTGLVIAASLVGLFVG
HHHHHHHHHHHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyric acid; (R)-1-aminopropan-2-yl phosphate; (R)-1-aminopropan-2-ol

Specific reaction: (1) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-yl phosphate = ADP + phosphate + adenosylcobinamide phosphate; (2) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-ol = ADP + phosphate + adenosylcobinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11756688