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The map label for this gene is ddlA [H]

Identifier: 225852765

GI number: 225852765

Start: 1253372

End: 1254433

Strand: Reverse

Name: ddlA [H]

Synonym: BMEA_A1317

Alternate gene names: 225852765

Gene position: 1254433-1253372 (Counterclockwise)

Preceding gene: 225852767

Following gene: 225852764

Centisome position: 59.01

GC content: 53.48

Gene sequence:

>1062_bases
ATGGCATCTGGAAAAATGCGTATCGTTGTCCTCTTTGGTGGACGCTCAGCGGAGCATGATGTTTCCGTTCTTTCAGCAAC
CAATGTCATGAATGCGCTGGATCCGGCCAAATATGAGGCTGTTCCTGTTTTTGTAACACGCGCGGGACAATGGCTGTTGA
GCCGCTTCGTGAACGGTGCGCTGGAAAAGCTGTCAAGCGGCGCAGAGCTTTGCCTTGTTCCGGGCGGCTGTGGGCGTGCC
ATCGTGGTTCCTGATGCCGGCGCTCCATACGAGGCGGACAAAATAGATATCATATTTCCGGTTCTGCATGGCCTTCATGG
GGAAGATGGTGCTGTGCAAGGCTTGGCTCAGGTTGCGCGAGTACCGCTGGCAGGTTGTGGTATTCCAGGTTCCGCCAATG
CACTCGACAAGGATATTGCCAAGCGCCTGGTCAATGAAGCGGGCCTGTCCACGGCAAAATCCGTGACCATCACGCGCGAA
GAGGTTCCCGCTTTCAGTGCGCTCGAACAGGCTTTGGGTTTGCCCATATTTATCAAACCCGCCCGTCAGGGTTCGTCTGT
CGGTGTCCACAAGGTTGTAACGGAAGCAGATTATCAGGCTGCCATGTCCGATGGTTTCATTTATGACGATAAGCTGCTGG
CTGAAGAGTTCATTCAGGCGCGCGAAGTTGAATGCGGCGTTCTTGAAGACGAAGGAGGCGCGCTGTTCGTCTCGCGCGCT
GGCGAGATTGTGCCTGCGGAAAGCCACTGTTTTTATAGTTATGATGCCAAATATATCGATGCCGATGGAACTGAGATCAA
GGTTCCTGCGGAGTTGCCCGAACAGGTAGAGAATGAAATCCGTGCGATCGCCGCCAAGGCTTTCCGGGTTCTGGGCTGCG
ATTCCATGGCGAGGGTTGATTTTTTTGTTACGGCAGACAGGCGTATCGTGCTGAATGAAATCAACACTATTCCCGGCTTC
ACCGATATGAGCATGTATTCCAAGGTGATGGCTGTGAGCGGTGTAAGCTATCCTGAAATTATCAATCGATTGGTTGCGCA
TGGCTTGGCGCGGGGAAGCTGA

Upstream 100 bases:

>100_bases
GCCGCGATATTTTAGGCCCTTGATGGGGACGACATTATATAGCCGAACTTGATCCGGATTTTTGAGAAGCAATCTTTGTT
CAAATTATATCGAGGTAGTC

Downstream 100 bases:

>100_bases
CTTTAATCTCATCATTGAAGAACGCTGGCTTTTGTGTCTTTAAGGACCAATGACAACAGAGCGTTTTTTCCTCGGAGTGA
AACAATCCGCAACCGGCCAG

Product: D-alanyl-alanine synthetase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase A; D-alanylalanine synthetaseA [H]

Number of amino acids: Translated: 353; Mature: 352

Protein sequence:

>353_residues
MASGKMRIVVLFGGRSAEHDVSVLSATNVMNALDPAKYEAVPVFVTRAGQWLLSRFVNGALEKLSSGAELCLVPGGCGRA
IVVPDAGAPYEADKIDIIFPVLHGLHGEDGAVQGLAQVARVPLAGCGIPGSANALDKDIAKRLVNEAGLSTAKSVTITRE
EVPAFSALEQALGLPIFIKPARQGSSVGVHKVVTEADYQAAMSDGFIYDDKLLAEEFIQAREVECGVLEDEGGALFVSRA
GEIVPAESHCFYSYDAKYIDADGTEIKVPAELPEQVENEIRAIAAKAFRVLGCDSMARVDFFVTADRRIVLNEINTIPGF
TDMSMYSKVMAVSGVSYPEIINRLVAHGLARGS

Sequences:

>Translated_353_residues
MASGKMRIVVLFGGRSAEHDVSVLSATNVMNALDPAKYEAVPVFVTRAGQWLLSRFVNGALEKLSSGAELCLVPGGCGRA
IVVPDAGAPYEADKIDIIFPVLHGLHGEDGAVQGLAQVARVPLAGCGIPGSANALDKDIAKRLVNEAGLSTAKSVTITRE
EVPAFSALEQALGLPIFIKPARQGSSVGVHKVVTEADYQAAMSDGFIYDDKLLAEEFIQAREVECGVLEDEGGALFVSRA
GEIVPAESHCFYSYDAKYIDADGTEIKVPAELPEQVENEIRAIAAKAFRVLGCDSMARVDFFVTADRRIVLNEINTIPGF
TDMSMYSKVMAVSGVSYPEIINRLVAHGLARGS
>Mature_352_residues
ASGKMRIVVLFGGRSAEHDVSVLSATNVMNALDPAKYEAVPVFVTRAGQWLLSRFVNGALEKLSSGAELCLVPGGCGRAI
VVPDAGAPYEADKIDIIFPVLHGLHGEDGAVQGLAQVARVPLAGCGIPGSANALDKDIAKRLVNEAGLSTAKSVTITREE
VPAFSALEQALGLPIFIKPARQGSSVGVHKVVTEADYQAAMSDGFIYDDKLLAEEFIQAREVECGVLEDEGGALFVSRAG
EIVPAESHCFYSYDAKYIDADGTEIKVPAELPEQVENEIRAIAAKAFRVLGCDSMARVDFFVTADRRIVLNEINTIPGFT
DMSMYSKVMAVSGVSYPEIINRLVAHGLARGS

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786579, Length=357, Percent_Identity=42.0168067226891, Blast_Score=292, Evalue=2e-80,
Organism=Escherichia coli, GI1786280, Length=355, Percent_Identity=30.4225352112676, Blast_Score=151, Evalue=8e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 37530; Mature: 37399

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1 ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASGKMRIVVLFGGRSAEHDVSVLSATNVMNALDPAKYEAVPVFVTRAGQWLLSRFVNGA
CCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCHHCEEEEEEEHHHHHHHHHHHHHH
LEKLSSGAELCLVPGGCGRAIVVPDAGAPYEADKIDIIFPVLHGLHGEDGAVQGLAQVAR
HHHHCCCCCEEEEECCCCCEEEECCCCCCCCCCCEEEEEHHHHCCCCCCHHHHHHHHHHH
VPLAGCGIPGSANALDKDIAKRLVNEAGLSTAKSVTITREEVPAFSALEQALGLPIFIKP
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHEEEEEHHHCCHHHHHHHHCCCCEEEEE
ARQGSSVGVHKVVTEADYQAAMSDGFIYDDKLLAEEFIQAREVECGVLEDEGGALFVSRA
CCCCCCCCHHHHHHHHHHHHHHCCCCEECHHHHHHHHHHHHCCCCCEEECCCCEEEEECC
GEIVPAESHCFYSYDAKYIDADGTEIKVPAELPEQVENEIRAIAAKAFRVLGCDSMARVD
CCEECCCCCEEEECCCEEEECCCCEEEECHHCHHHHHHHHHHHHHHHHHHHCCCCHHEEE
FFVTADRRIVLNEINTIPGFTDMSMYSKVMAVSGVSYPEIINRLVAHGLARGS
EEEECCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ASGKMRIVVLFGGRSAEHDVSVLSATNVMNALDPAKYEAVPVFVTRAGQWLLSRFVNGA
CCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCHHCEEEEEEEHHHHHHHHHHHHHH
LEKLSSGAELCLVPGGCGRAIVVPDAGAPYEADKIDIIFPVLHGLHGEDGAVQGLAQVAR
HHHHCCCCCEEEEECCCCCEEEECCCCCCCCCCCEEEEEHHHHCCCCCCHHHHHHHHHHH
VPLAGCGIPGSANALDKDIAKRLVNEAGLSTAKSVTITREEVPAFSALEQALGLPIFIKP
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHEEEEEHHHCCHHHHHHHHCCCCEEEEE
ARQGSSVGVHKVVTEADYQAAMSDGFIYDDKLLAEEFIQAREVECGVLEDEGGALFVSRA
CCCCCCCCHHHHHHHHHHHHHHCCCCEECHHHHHHHHHHHHCCCCCEEECCCCEEEEECC
GEIVPAESHCFYSYDAKYIDADGTEIKVPAELPEQVENEIRAIAAKAFRVLGCDSMARVD
CCEECCCCCEEEECCCEEEECCCCEEEECHHCHHHHHHHHHHHHHHHHHHHCCCCHHEEE
FFVTADRRIVLNEINTIPGFTDMSMYSKVMAVSGVSYPEIINRLVAHGLARGS
EEEECCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]