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The map label for this gene is rlmB [H]

Identifier: 225852749

GI number: 225852749

Start: 1240393

End: 1241253

Strand: Direct

Name: rlmB [H]

Synonym: BMEA_A1300

Alternate gene names: 225852749

Gene position: 1240393-1241253 (Clockwise)

Preceding gene: 225852748

Following gene: 225852752

Centisome position: 58.35

GC content: 61.32

Gene sequence:

>861_bases
ATGACCGATCAAAATTCGCCCCGTACACCCAAAGATTCTCACTATGCGCGCCTGCGTCGCCAGCACCGCGACCAGAAGGC
CGGAGCGGAAGGCAAATCACCACGCAAACCGCAGCGCCCCGCGATCGCGGCAAGCGCGGTTGCCGAAGGAACCGTGCGCC
TTTATGGCCTGCATACGGTTCGCGCCGCCGTGGAAAACCCGCAGCGCAAAATCCTGCGTATGCGCGCCACACGCAACGGC
TTTGCCCGGCTTGAGATTGGCGAAGCCGATTCTCTCCCCTTCCCCGTGGAGATCGTCGATCCGAGGGATATCGACAAGGA
AACCGGGTCGGAAGCCGTGCATCAGGGCGTCATGATCGAGGCCGAACCGCTTCGCACCAGAAAACTTTCCGAACTCAGGG
AAAGCCCGCTGCTTCTGGTGCTCGACCAGGTGACGGACCCGCATAATGTCGGCGCGATCATGCGCTCGGCAGTCGCTTTC
GGCGCTGGCGCCCTGATTACGACCAGCCGCCACAGCCCGCAGGAAACCGGCGTTCTGGCCAAGGCAGCCTCCGGCGCATT
GGAGCTCATTGCCCATATCGAGGTTCGCAATCTCGCCGAAGCCATCGAAGAACTGCACACGCTTGGCTTCCAGACCATCG
GTCTTGATTCGCAAGGGCCTCTGGAGATGGAGGCGACGCTTTCAGGAAACCGAATCGCTCTGGTGCTGGGCGCCGAAGGC
AAGGGCTTGCGCCAGAAAACGCGCGAAACCGTGACAGCACTTGCCCGCCTCGACATGCCGGGCGAGATCAAGTCGCTCAA
TGTTTCCAATGCGGCGGCAATTTCGCTTTACGCAGCCGAACGTTATCTGCGTTCACGCTGA

Upstream 100 bases:

>100_bases
TTTGGCGGGCTTTGCGAATTATTTTACAATCGGAACAGCCTGATAGCATTTGCAGGGCAAGCCGCTATCTTCCGCCCCTC
TAAAGAGCTATAAGCGCACC

Downstream 100 bases:

>100_bases
TTCGTACTTTCTGAGACCATGCCTCCCGACAGGGAAGGCATGGCAGCGAATCATCCGGCTTCAGGCCGTCGCGATATAGG
TGCGAATATCTTCCGCCTCG

Product: TrmH family RNA methyltransferase

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MTDQNSPRTPKDSHYARLRRQHRDQKAGAEGKSPRKPQRPAIAASAVAEGTVRLYGLHTVRAAVENPQRKILRMRATRNG
FARLEIGEADSLPFPVEIVDPRDIDKETGSEAVHQGVMIEAEPLRTRKLSELRESPLLLVLDQVTDPHNVGAIMRSAVAF
GAGALITTSRHSPQETGVLAKAASGALELIAHIEVRNLAEAIEELHTLGFQTIGLDSQGPLEMEATLSGNRIALVLGAEG
KGLRQKTRETVTALARLDMPGEIKSLNVSNAAAISLYAAERYLRSR

Sequences:

>Translated_286_residues
MTDQNSPRTPKDSHYARLRRQHRDQKAGAEGKSPRKPQRPAIAASAVAEGTVRLYGLHTVRAAVENPQRKILRMRATRNG
FARLEIGEADSLPFPVEIVDPRDIDKETGSEAVHQGVMIEAEPLRTRKLSELRESPLLLVLDQVTDPHNVGAIMRSAVAF
GAGALITTSRHSPQETGVLAKAASGALELIAHIEVRNLAEAIEELHTLGFQTIGLDSQGPLEMEATLSGNRIALVLGAEG
KGLRQKTRETVTALARLDMPGEIKSLNVSNAAAISLYAAERYLRSR
>Mature_285_residues
TDQNSPRTPKDSHYARLRRQHRDQKAGAEGKSPRKPQRPAIAASAVAEGTVRLYGLHTVRAAVENPQRKILRMRATRNGF
ARLEIGEADSLPFPVEIVDPRDIDKETGSEAVHQGVMIEAEPLRTRKLSELRESPLLLVLDQVTDPHNVGAIMRSAVAFG
AGALITTSRHSPQETGVLAKAASGALELIAHIEVRNLAEAIEELHTLGFQTIGLDSQGPLEMEATLSGNRIALVLGAEGK
GLRQKTRETVTALARLDMPGEIKSLNVSNAAAISLYAAERYLRSR

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=255, Percent_Identity=32.156862745098, Blast_Score=89, Evalue=5e-18,
Organism=Escherichia coli, GI1790623, Length=239, Percent_Identity=33.8912133891213, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1788935, Length=183, Percent_Identity=25.6830601092896, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17555908, Length=282, Percent_Identity=25.886524822695, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324775, Length=338, Percent_Identity=24.5562130177515, Blast_Score=77, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 31076; Mature: 30945

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDQNSPRTPKDSHYARLRRQHRDQKAGAEGKSPRKPQRPAIAASAVAEGTVRLYGLHTV
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCEEEEEHHHH
RAAVENPQRKILRMRATRNGFARLEIGEADSLPFPVEIVDPRDIDKETGSEAVHQGVMIE
HHHHHCHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEECCCCCCHHCCHHHHHCCEEEE
AEPLRTRKLSELRESPLLLVLDQVTDPHNVGAIMRSAVAFGAGALITTSRHSPQETGVLA
CCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHH
KAASGALELIAHIEVRNLAEAIEELHTLGFQTIGLDSQGPLEMEATLSGNRIALVLGAEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCEEEEEEECCCEEEEEECCCC
KGLRQKTRETVTALARLDMPGEIKSLNVSNAAAISLYAAERYLRSR
CCHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TDQNSPRTPKDSHYARLRRQHRDQKAGAEGKSPRKPQRPAIAASAVAEGTVRLYGLHTV
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCEEEEEHHHH
RAAVENPQRKILRMRATRNGFARLEIGEADSLPFPVEIVDPRDIDKETGSEAVHQGVMIE
HHHHHCHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEECCCCCCHHCCHHHHHCCEEEE
AEPLRTRKLSELRESPLLLVLDQVTDPHNVGAIMRSAVAFGAGALITTSRHSPQETGVLA
CCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHH
KAASGALELIAHIEVRNLAEAIEELHTLGFQTIGLDSQGPLEMEATLSGNRIALVLGAEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCEEEEEEECCCEEEEEECCCC
KGLRQKTRETVTALARLDMPGEIKSLNVSNAAAISLYAAERYLRSR
CCHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]