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The map label for this gene is dacA [H]

Identifier: 225852669

GI number: 225852669

Start: 1169708

End: 1171126

Strand: Direct

Name: dacA [H]

Synonym: BMEA_A1215

Alternate gene names: 225852669

Gene position: 1169708-1171126 (Clockwise)

Preceding gene: 225852668

Following gene: 225852670

Centisome position: 55.03

GC content: 61.31

Gene sequence:

>1419_bases
GTGCGTACCGCATTCAGTAAAGTCGCATATGCCTTGAAAAAAGCGGCACAGGTCTCTCTTCTTCTAACAGCCGTGACGGG
TTACTCCACGGTTTCCTCCATTGCACCGGCCATGGCGAAAGGCGACAACAGATATGCCGCGATCGTCGTTGATGCGAACA
CCGGCAAGACGCTTTTCTCGGCAAATGCCGATGCACAGCGCTATCCGGCGTCGCTGACCAAGATGATGACGCTCTATATG
CTGTTTGAAGCCATCCACGCCGGCCGCATCAACAAGGATACGCGCATTCCCGTGTCGGCCTATGCCGCCTCCCGTCCGCC
CACGAAGATCGGCTTTCGTCCCGGCCAGTCGATTCGGGCGGAAGATGCGGCCCTTGCGATGATTACGAAATCCGCAAACG
ACGTTGCTGCGGCAGTCGGTGAATATCTTGGCGGCTCGGAAGAACGTTTCGCCCAGATGATGACCGCAAGGGCGCGCCGC
CTTGGTATGCGCAACACGACCTTCCGCAATGCGTCCGGCCTGCCGAACATGGCCCAGCATTCGTCCGCCCGCGACATGGC
CATCCTTGGCATGGCGCTGCGTCGCCATTTCCCGCGTGAATTTGCCTATTTCTCGCGCAGCAGCTTCGATTTCCGCGGCC
AAACCATCCGTGGCCATAACCGGCTTCTTGGCCGTGTCGAAGGCGTGGACGGCATCAAGACGGGCTATACCAACGCTTCC
GGTTACAACCTTGTTTCGTCAGTCAATCTCGATGGTCGCCGTCTCGTCGCCGTCGTGATGGGCGGCAATACCGGCGCAAG
CCGCGATGCCCACATGGCCCAGCTTATTCGCAAATATCTGCCGATGGCCGCCCGTGGCCGTAATAACGATGCTTTGATTG
CCGCGCGCAGTGAAGCCCCGCAGGTTGTGGCTTCCATCGACCTGCCAAAAACCAAACGTGCACCGGTGCCCGTCGCCCGC
ATCGCCGTCGAGGACATCATCAACGCCGAAATCGGTGAAGGTGATATCGACCAGCCGCAGGTTCTGGCCCTCGTAGCCCC
CACCCCGCGCCCGGCGGCTCTGGCCGCTCAGGCAGCCGTACTTCCGACGCCGAAGGCCCCCGTTCGGCCAACGCAGGCCC
CGGCACAAGATGTGGATCCGGTGACAACGGCATCCGCATCGGCTGCAAGCGGCTGGGCGATCCAGATCGGCTCGCTCCCG
AGCGAAGGACAGGCCCGCGATATGCTGGCCAAGGCGTCCGCCACTGCTGGCCGCACACTGCGCTCCGCTTCGCCCTATAC
GGAAACCTTCAACAAGGGTAGCGCCACCTTCTATCGCGCCCGTTTCGTCGGCTTTACCTCCAAGCAGGCGGCCTGGGATG
CTTGCGCCTCGCTGAAGCGCAATAACTTCGGCTGCTATGCGGTAGCGAACTCGAACTGA

Upstream 100 bases:

>100_bases
ACGGATTGGTAACGCTGATGGCCGTAAGGTGTCCGAATTAGGTTGCGGTGTGCTTTCACCGCCGCAGTAAGAAATTCCGG
GATACAGGCAGGTCATCATC

Downstream 100 bases:

>100_bases
ATTCACGAAAGTTGCAAGGGGAAACCTCCCCTTGCTGCACCTTCAAACCCCCATTTTGTATAGCGTAAAGGGTTTCGAGA
TGTCCAATATGAAAGAGACT

Product: serine-type D-Ala-D-Ala carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MRTAFSKVAYALKKAAQVSLLLTAVTGYSTVSSIAPAMAKGDNRYAAIVVDANTGKTLFSANADAQRYPASLTKMMTLYM
LFEAIHAGRINKDTRIPVSAYAASRPPTKIGFRPGQSIRAEDAALAMITKSANDVAAAVGEYLGGSEERFAQMMTARARR
LGMRNTTFRNASGLPNMAQHSSARDMAILGMALRRHFPREFAYFSRSSFDFRGQTIRGHNRLLGRVEGVDGIKTGYTNAS
GYNLVSSVNLDGRRLVAVVMGGNTGASRDAHMAQLIRKYLPMAARGRNNDALIAARSEAPQVVASIDLPKTKRAPVPVAR
IAVEDIINAEIGEGDIDQPQVLALVAPTPRPAALAAQAAVLPTPKAPVRPTQAPAQDVDPVTTASASAASGWAIQIGSLP
SEGQARDMLAKASATAGRTLRSASPYTETFNKGSATFYRARFVGFTSKQAAWDACASLKRNNFGCYAVANSN

Sequences:

>Translated_472_residues
MRTAFSKVAYALKKAAQVSLLLTAVTGYSTVSSIAPAMAKGDNRYAAIVVDANTGKTLFSANADAQRYPASLTKMMTLYM
LFEAIHAGRINKDTRIPVSAYAASRPPTKIGFRPGQSIRAEDAALAMITKSANDVAAAVGEYLGGSEERFAQMMTARARR
LGMRNTTFRNASGLPNMAQHSSARDMAILGMALRRHFPREFAYFSRSSFDFRGQTIRGHNRLLGRVEGVDGIKTGYTNAS
GYNLVSSVNLDGRRLVAVVMGGNTGASRDAHMAQLIRKYLPMAARGRNNDALIAARSEAPQVVASIDLPKTKRAPVPVAR
IAVEDIINAEIGEGDIDQPQVLALVAPTPRPAALAAQAAVLPTPKAPVRPTQAPAQDVDPVTTASASAASGWAIQIGSLP
SEGQARDMLAKASATAGRTLRSASPYTETFNKGSATFYRARFVGFTSKQAAWDACASLKRNNFGCYAVANSN
>Mature_472_residues
MRTAFSKVAYALKKAAQVSLLLTAVTGYSTVSSIAPAMAKGDNRYAAIVVDANTGKTLFSANADAQRYPASLTKMMTLYM
LFEAIHAGRINKDTRIPVSAYAASRPPTKIGFRPGQSIRAEDAALAMITKSANDVAAAVGEYLGGSEERFAQMMTARARR
LGMRNTTFRNASGLPNMAQHSSARDMAILGMALRRHFPREFAYFSRSSFDFRGQTIRGHNRLLGRVEGVDGIKTGYTNAS
GYNLVSSVNLDGRRLVAVVMGGNTGASRDAHMAQLIRKYLPMAARGRNNDALIAARSEAPQVVASIDLPKTKRAPVPVAR
IAVEDIINAEIGEGDIDQPQVLALVAPTPRPAALAAQAAVLPTPKAPVRPTQAPAQDVDPVTTASASAASGWAIQIGSLP
SEGQARDMLAKASATAGRTLRSASPYTETFNKGSATFYRARFVGFTSKQAAWDACASLKRNNFGCYAVANSN

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein. Note=N-terminal lies in the periplasmic space (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1786851, Length=290, Percent_Identity=35.1724137931034, Blast_Score=159, Evalue=4e-40,
Organism=Escherichia coli, GI1787062, Length=265, Percent_Identity=36.6037735849057, Blast_Score=148, Evalue=8e-37,
Organism=Escherichia coli, GI87082022, Length=238, Percent_Identity=35.2941176470588, Blast_Score=145, Evalue=6e-36,
Organism=Escherichia coli, GI87082054, Length=239, Percent_Identity=29.7071129707113, Blast_Score=82, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 50211; Mature: 50211

Theoretical pI: Translated: 10.83; Mature: 10.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTAFSKVAYALKKAAQVSLLLTAVTGYSTVSSIAPAMAKGDNRYAAIVVDANTGKTLFS
CCCHHHHHHHHHHHHHHHEEEEHHHHCHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEE
ANADAQRYPASLTKMMTLYMLFEAIHAGRINKDTRIPVSAYAASRPPTKIGFRPGQSIRA
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCC
EDAALAMITKSANDVAAAVGEYLGGSEERFAQMMTARARRLGMRNTTFRNASGLPNMAQH
CCCEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHH
SSARDMAILGMALRRHFPREFAYFSRSSFDFRGQTIRGHNRLLGRVEGVDGIKTGYTNAS
CCCCHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCEECCCHHHHHHHCCCCCCCCCCCCCC
GYNLVSSVNLDGRRLVAVVMGGNTGASRDAHMAQLIRKYLPMAARGRNNDALIAARSEAP
CCCEEEECCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCHHHCCCCCCEEEEECCCCC
QVVASIDLPKTKRAPVPVARIAVEDIINAEIGEGDIDQPQVLALVAPTPRPAALAAQAAV
EEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHC
LPTPKAPVRPTQAPAQDVDPVTTASASAASGWAIQIGSLPSEGQARDMLAKASATAGRTL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCHH
RSASPYTETFNKGSATFYRARFVGFTSKQAAWDACASLKRNNFGCYAVANSN
CCCCCCHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEECCC
>Mature Secondary Structure
MRTAFSKVAYALKKAAQVSLLLTAVTGYSTVSSIAPAMAKGDNRYAAIVVDANTGKTLFS
CCCHHHHHHHHHHHHHHHEEEEHHHHCHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEE
ANADAQRYPASLTKMMTLYMLFEAIHAGRINKDTRIPVSAYAASRPPTKIGFRPGQSIRA
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCC
EDAALAMITKSANDVAAAVGEYLGGSEERFAQMMTARARRLGMRNTTFRNASGLPNMAQH
CCCEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHH
SSARDMAILGMALRRHFPREFAYFSRSSFDFRGQTIRGHNRLLGRVEGVDGIKTGYTNAS
CCCCHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCEECCCHHHHHHHCCCCCCCCCCCCCC
GYNLVSSVNLDGRRLVAVVMGGNTGASRDAHMAQLIRKYLPMAARGRNNDALIAARSEAP
CCCEEEECCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCHHHCCCCCCEEEEECCCCC
QVVASIDLPKTKRAPVPVARIAVEDIINAEIGEGDIDQPQVLALVAPTPRPAALAAQAAV
EEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHC
LPTPKAPVRPTQAPAQDVDPVTTASASAASGWAIQIGSLPSEGQARDMLAKASATAGRTL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCHH
RSASPYTETFNKGSATFYRARFVGFTSKQAAWDACASLKRNNFGCYAVANSN
CCCCCCHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]