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The map label for this gene is pdhB [H]

Identifier: 225852628

GI number: 225852628

Start: 1124809

End: 1126194

Strand: Reverse

Name: pdhB [H]

Synonym: BMEA_A1173

Alternate gene names: 225852628

Gene position: 1126194-1124809 (Counterclockwise)

Preceding gene: 225852629

Following gene: 225852627

Centisome position: 52.98

GC content: 57.65

Gene sequence:

>1386_bases
ATGCCCATAGAAATTCTCATGCCCGCACTTTCCCCGACCATGGAGGAGGGCAAGCTCTCCAAATGGCTCAAGAAAGAAGG
CGACAAGGTTACGTCCGGCGATGTGATCGCCGAAATCGAGACCGACAAGGCGACGATGGAAGTCGAAGCTGTTGATGAAG
GCACCATTGGCAAACTTCTTGTCGATGAAGGCACCGAAGGCGTGAAGGTCAATACGCCGATTGCCGTGCTTCTTGGCGAC
GGCGAGAGTGCTGCCGATATCGGTTCTGCTCCGGCTGCAAAGGCCGAGGCGGCGAAGGAAGAGCCGAAGGCGGAAGAAAA
CAAGGCCGATGCCGTTCCTGCCGCTCCAAAGGCTCCGGCTGTTGAAGTTGCGTCCGACCCGGATATTCCGGCTGGCACGG
AAATGGTTTCCTTGACTGTTCGCGAAGCTCTTCGCGATGCCATGGCGGAAGAAATGCGCCGCGATCCCGATGTCTTCATC
ATGGGTGAGGAAGTCGCCCAATATCAGGGCGCCTACAAGATCACGCAGGGGCTTCTGGATGAATTTGGTCCCAAGCGCGT
CGTCGATACACCGATTACGGAACATGGCTTTGCCGGTGTGGGCGTTGGTGCTGCTTTTGCCGGCCTGAAGCCGATCGTTG
AATTCATGACCTTCAACTTCGCCATGCAGGCAATTGACCAGATCGTGAATTCCGCCGCCAAGACGCTTTACATGTCGGGT
GGCCAGATGGGCGCGCCGATGGTTTTCCGCGGCCCTTCGGGCGCGGCAGCCCGCGTCGCCGCGCAGCACTCGCAGTGCTA
TGCCGCCTGGTACAGCCATATTCCGGGCCTGAAGGTCGTGATGCCCTATACGGCAGCCGATGCGAAGGGCCTTCTCAAGG
CTGCGATCCGCGATCCGAATCCGGTCATCTTCCTTGAAAATGAAATTCTCTACGGCCATCATTTCGATGTGCCGAAGCTT
GATGATTTCGTTCTGCCGATTGGCAAGGCGCGGATCCACAAGCAGGGCAAGGATGCAACAATCGTCTCGTTCGGCATCGG
CATGACCTATGCCGTGAAGGCAGCGGAAGAGCTTGCCGGGCAGGGCATTGATGTGGAAATCATTGACCTGCGCACCATTC
GCCCGATGGATATTGCGACGGTGGTGGAATCGGTCAAGAAGACGGGCCGCCTCGTGACGGTGGAAGAAGGCTTCCCGCAG
TCGTCTGTCGGCACGGAAATCGCCACCCGCGTGATGCAGCAGGCCTTCGATTATCTCGATGCGCCAATCCTGACTATCGC
TGGCAAGGACGTTCCGATGCCTTATGCCGCAAATCTTGAAAAGCTGGCGCTTCCGAGCGTTGCCGAAGTGGTCGAAGCGG
TGAAAGCCGTTACCTATACCGCTTAA

Upstream 100 bases:

>100_bases
CATTGTCGCTGATGCTGCCGATTTTGCCGAACATGATCCGGAGCCGGATGCGTCCGAGCTCTATACGGATATTCTGCTCT
AATTCGAGGAAGGTGTTGCT

Downstream 100 bases:

>100_bases
ACAGAAAGGGTCTGGACATGCCGATCAATATCACCATGCCAGCGCTTTCTCCCACGATGGAAGAAGGTAACCTGTCGAAA
TGGCTGGTCAAGGAAGGCGA

Product: pyruvate dehydrogenase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MPIEILMPALSPTMEEGKLSKWLKKEGDKVTSGDVIAEIETDKATMEVEAVDEGTIGKLLVDEGTEGVKVNTPIAVLLGD
GESAADIGSAPAAKAEAAKEEPKAEENKADAVPAAPKAPAVEVASDPDIPAGTEMVSLTVREALRDAMAEEMRRDPDVFI
MGEEVAQYQGAYKITQGLLDEFGPKRVVDTPITEHGFAGVGVGAAFAGLKPIVEFMTFNFAMQAIDQIVNSAAKTLYMSG
GQMGAPMVFRGPSGAAARVAAQHSQCYAAWYSHIPGLKVVMPYTAADAKGLLKAAIRDPNPVIFLENEILYGHHFDVPKL
DDFVLPIGKARIHKQGKDATIVSFGIGMTYAVKAAEELAGQGIDVEIIDLRTIRPMDIATVVESVKKTGRLVTVEEGFPQ
SSVGTEIATRVMQQAFDYLDAPILTIAGKDVPMPYAANLEKLALPSVAEVVEAVKAVTYTA

Sequences:

>Translated_461_residues
MPIEILMPALSPTMEEGKLSKWLKKEGDKVTSGDVIAEIETDKATMEVEAVDEGTIGKLLVDEGTEGVKVNTPIAVLLGD
GESAADIGSAPAAKAEAAKEEPKAEENKADAVPAAPKAPAVEVASDPDIPAGTEMVSLTVREALRDAMAEEMRRDPDVFI
MGEEVAQYQGAYKITQGLLDEFGPKRVVDTPITEHGFAGVGVGAAFAGLKPIVEFMTFNFAMQAIDQIVNSAAKTLYMSG
GQMGAPMVFRGPSGAAARVAAQHSQCYAAWYSHIPGLKVVMPYTAADAKGLLKAAIRDPNPVIFLENEILYGHHFDVPKL
DDFVLPIGKARIHKQGKDATIVSFGIGMTYAVKAAEELAGQGIDVEIIDLRTIRPMDIATVVESVKKTGRLVTVEEGFPQ
SSVGTEIATRVMQQAFDYLDAPILTIAGKDVPMPYAANLEKLALPSVAEVVEAVKAVTYTA
>Mature_460_residues
PIEILMPALSPTMEEGKLSKWLKKEGDKVTSGDVIAEIETDKATMEVEAVDEGTIGKLLVDEGTEGVKVNTPIAVLLGDG
ESAADIGSAPAAKAEAAKEEPKAEENKADAVPAAPKAPAVEVASDPDIPAGTEMVSLTVREALRDAMAEEMRRDPDVFIM
GEEVAQYQGAYKITQGLLDEFGPKRVVDTPITEHGFAGVGVGAAFAGLKPIVEFMTFNFAMQAIDQIVNSAAKTLYMSGG
QMGAPMVFRGPSGAAARVAAQHSQCYAAWYSHIPGLKVVMPYTAADAKGLLKAAIRDPNPVIFLENEILYGHHFDVPKLD
DFVLPIGKARIHKQGKDATIVSFGIGMTYAVKAAEELAGQGIDVEIIDLRTIRPMDIATVVESVKKTGRLVTVEEGFPQS
SVGTEIATRVMQQAFDYLDAPILTIAGKDVPMPYAANLEKLALPSVAEVVEAVKAVTYTA

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156564403, Length=324, Percent_Identity=56.7901234567901, Blast_Score=394, Evalue=1e-109,
Organism=Homo sapiens, GI291084858, Length=324, Percent_Identity=53.7037037037037, Blast_Score=363, Evalue=1e-100,
Organism=Homo sapiens, GI4557353, Length=333, Percent_Identity=33.9339339339339, Blast_Score=204, Evalue=2e-52,
Organism=Homo sapiens, GI34101272, Length=333, Percent_Identity=33.9339339339339, Blast_Score=204, Evalue=2e-52,
Organism=Homo sapiens, GI203098753, Length=81, Percent_Identity=50.6172839506173, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI203098816, Length=81, Percent_Identity=50.6172839506173, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI31711992, Length=87, Percent_Identity=47.1264367816092, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI260898739, Length=59, Percent_Identity=59.3220338983051, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI225637461, Length=256, Percent_Identity=28.515625, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI225637459, Length=256, Percent_Identity=28.515625, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI225637463, Length=307, Percent_Identity=27.0358306188925, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI133778974, Length=246, Percent_Identity=27.2357723577236, Blast_Score=69, Evalue=9e-12,
Organism=Homo sapiens, GI205277463, Length=320, Percent_Identity=24.0625, Blast_Score=66, Evalue=6e-11,
Organism=Homo sapiens, GI4507521, Length=320, Percent_Identity=24.0625, Blast_Score=66, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17538422, Length=323, Percent_Identity=61.9195046439629, Blast_Score=412, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI17506935, Length=339, Percent_Identity=40.7079646017699, Blast_Score=201, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI17560088, Length=128, Percent_Identity=36.71875, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17539652, Length=257, Percent_Identity=29.9610894941634, Blast_Score=65, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6319698, Length=326, Percent_Identity=58.8957055214724, Blast_Score=397, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6324258, Length=131, Percent_Identity=42.7480916030534, Blast_Score=94, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6321632, Length=71, Percent_Identity=50.7042253521127, Blast_Score=78, Evalue=3e-15,
Organism=Drosophila melanogaster, GI21358145, Length=320, Percent_Identity=60, Blast_Score=404, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24650940, Length=320, Percent_Identity=60, Blast_Score=404, Evalue=1e-112,
Organism=Drosophila melanogaster, GI160714832, Length=322, Percent_Identity=34.1614906832298, Blast_Score=197, Evalue=1e-50,
Organism=Drosophila melanogaster, GI160714828, Length=322, Percent_Identity=34.1614906832298, Blast_Score=196, Evalue=3e-50,
Organism=Drosophila melanogaster, GI24650943, Length=91, Percent_Identity=63.7362637362637, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24650945, Length=91, Percent_Identity=63.7362637362637, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI20129315, Length=83, Percent_Identity=48.1927710843374, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI45551847, Length=241, Percent_Identity=26.9709543568465, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI45550715, Length=241, Percent_Identity=26.9709543568465, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24645119, Length=241, Percent_Identity=26.9709543568465, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24582497, Length=72, Percent_Identity=45.8333333333333, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR000089
- InterPro:   IPR011053
- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 48955; Mature: 48823

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIEILMPALSPTMEEGKLSKWLKKEGDKVTSGDVIAEIETDKATMEVEAVDEGTIGKLL
CCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCEEEEEEECCCCCCCEEE
VDEGTEGVKVNTPIAVLLGDGESAADIGSAPAAKAEAAKEEPKAEENKADAVPAAPKAPA
ECCCCCCEEECCCEEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCE
VEVASDPDIPAGTEMVSLTVREALRDAMAEEMRRDPDVFIMGEEVAQYQGAYKITQGLLD
EEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHCCHHHHHHHHHH
EFGPKRVVDTPITEHGFAGVGVGAAFAGLKPIVEFMTFNFAMQAIDQIVNSAAKTLYMSG
HCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECC
GQMGAPMVFRGPSGAAARVAAQHSQCYAAWYSHIPGLKVVMPYTAADAKGLLKAAIRDPN
CCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCC
PVIFLENEILYGHHFDVPKLDDFVLPIGKARIHKQGKDATIVSFGIGMTYAVKAAEELAG
CEEEEECCEEECCCCCCCCCCCHHCCCCHHHHHCCCCCCEEEEECCCHHHHHHHHHHHCC
QGIDVEIIDLRTIRPMDIATVVESVKKTGRLVTVEEGFPQSSVGTEIATRVMQQAFDYLD
CCCCEEEEEECCCCCCHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHC
APILTIAGKDVPMPYAANLEKLALPSVAEVVEAVKAVTYTA
CCEEEEECCCCCCCHHCCHHHHCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PIEILMPALSPTMEEGKLSKWLKKEGDKVTSGDVIAEIETDKATMEVEAVDEGTIGKLL
CCEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCEEEEEEECCCCCCCEEE
VDEGTEGVKVNTPIAVLLGDGESAADIGSAPAAKAEAAKEEPKAEENKADAVPAAPKAPA
ECCCCCCEEECCCEEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCE
VEVASDPDIPAGTEMVSLTVREALRDAMAEEMRRDPDVFIMGEEVAQYQGAYKITQGLLD
EEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHCCHHHHHHHHHH
EFGPKRVVDTPITEHGFAGVGVGAAFAGLKPIVEFMTFNFAMQAIDQIVNSAAKTLYMSG
HCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECC
GQMGAPMVFRGPSGAAARVAAQHSQCYAAWYSHIPGLKVVMPYTAADAKGLLKAAIRDPN
CCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCC
PVIFLENEILYGHHFDVPKLDDFVLPIGKARIHKQGKDATIVSFGIGMTYAVKAAEELAG
CEEEEECCEEECCCCCCCCCCCHHCCCCHHHHHCCCCCCEEEEECCCHHHHHHHHHHHCC
QGIDVEIIDLRTIRPMDIATVVESVKKTGRLVTVEEGFPQSSVGTEIATRVMQQAFDYLD
CCCCEEEEEECCCCCCHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHC
APILTIAGKDVPMPYAANLEKLALPSVAEVVEAVKAVTYTA
CCEEEEECCCCCCCHHCCHHHHCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10796014; 11481430 [H]