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The map label for this gene is lpdA [H]

Identifier: 225852626

GI number: 225852626

Start: 1121902

End: 1123365

Strand: Reverse

Name: lpdA [H]

Synonym: BMEA_A1171

Alternate gene names: 225852626

Gene position: 1123365-1121902 (Counterclockwise)

Preceding gene: 225852627

Following gene: 225852625

Centisome position: 52.85

GC content: 58.81

Gene sequence:

>1464_bases
ATGGCCGACATTTACGACGTTATTGTTATCGGATCTGGCCCCGGCGGCTATGTGACGGCGATCCGCGCAGCGCAGCTCGG
TCTCAAGACCGCTATCGTCGAACGCGAACATCTGGGCGGCATCTGCCTCAACTGGGGCTGTATTCCGACCAAGGCGCTGT
TGCGTTCGGCGGAAATCCTGCATTTCGGCGAACATGCCAAGGATTACGGCCTGAAACTCGACGGCACCATCACGCCTGAC
GTGAAAGCTGTTGTGCAGCGTTCGCGCGGGGTTTCGGCGCGGCTGAATGGCGGCGTTGCCTTCCTGATGAAGAAAAACAA
GATCGATGTGATCTGGGGCGAGGCGAAGCTTGTGAAGGCTGCCTCCGGCAGCAATCCGGCGGAGATTTCCGTCGGCAAGA
TCTCCAAACAGCCAATGCAGCCGCAAAACCCCGTGCCGAAGGGCGTGCTGGGCGAGGGCAGCTATAAGGCCAAGCATATC
ATCGTGGCCACGGGTGCGCGCCCGCGGGCGCTGCCCGGTATCGAGCCGGACGGCAAATTGATCTGGACCTATTTTGAAGC
CATGGTGCCGCAGGCCCTGCCGAAATCCATGCTGGTGATGGGGTCTGGCGCTATCGGTATCGAATTTGCCTCCTTCTATC
ACGACATGGGTGTCGATGTGACCGTGGTGGAAGTGATGTCGCAGATCATGCCGGTGGAAGATGCCGAAACCTCTGCCATT
GCCCGCAAGCAGCTTGAAAAGCGCGGCCTGAAGATCATCACCGACGCCAAGGTGGCAAAGGTGGAAAAGGGCGCCAATAG
CGTCACGGCCCATGTCGAGACCAAGGATGGCAAAACCCAGACCCTGACGGTTGACCGCATGATCTCGGCGGTGGGTGTGC
AGGGCAATATCGAAAATCTCGGTCTGGAAGCACTTGGCGTGAAGACGGATCGCGGCTGCATCGTCATCGACGGTTATGGC
AAGACCAATGTGGCGGGCATCTATGCCATTGGCGATGTTGCCGGGCCGCCGATGCTGGCGCACAAGGCAGAGCATGAAGG
CGTGATCTGCGTGGAAAAGATTGCGGGCCTGCCCAATGTCCATCCGCTGGACATGGGCAAGATTCCCGGCTGCACCTATT
GCAACCCGCAGGTGGCCTCGGTCGGCCTGACTGAAGCCAAGGCGAAAGAGCAGGGCTATGACATTCGTGTCGGGCGCTAT
TCCTTCTCGGCCAATGGCAAGGCGATCGCGCTTGGTGAGGATCAGGGGCTGGTCAAGACCATCTTCGACCGGAAGACCGG
ACAGCTTTTGGGTGCGCATATGGTGGGTGCGGAAGTGACTGAACTCATTCAGGGCTTCGTGATCGCCATGAACCTTGAAA
CCACCGAAGAGGAACTGATGCATTCGGTCTTCCCGCATCCGACCCTTTCGGAAACGATGAAGGAAAGCGTTCTCGACGCC
TATGGACGGGTGCTGAACGCTTGA

Upstream 100 bases:

>100_bases
ATGGGTATGCTGGTCTGATGGAACTGACGCCGCGCTTGTCTTTGTAGCTTATACGAAGAACAGCGCGGCCTTTCCGTTTT
CAGGAATAAGGGCGAAAATT

Downstream 100 bases:

>100_bases
TGGGGGAGGTTCGCACATTCCGCTGATCGAGCATGGAGGGCCATGGCGGCGCGCCGCCTGGCCGGTCATAAAATGGTGTA
GCGTGGCGGGACTGGTCCTT

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes [H]

Number of amino acids: Translated: 487; Mature: 486

Protein sequence:

>487_residues
MADIYDVIVIGSGPGGYVTAIRAAQLGLKTAIVEREHLGGICLNWGCIPTKALLRSAEILHFGEHAKDYGLKLDGTITPD
VKAVVQRSRGVSARLNGGVAFLMKKNKIDVIWGEAKLVKAASGSNPAEISVGKISKQPMQPQNPVPKGVLGEGSYKAKHI
IVATGARPRALPGIEPDGKLIWTYFEAMVPQALPKSMLVMGSGAIGIEFASFYHDMGVDVTVVEVMSQIMPVEDAETSAI
ARKQLEKRGLKIITDAKVAKVEKGANSVTAHVETKDGKTQTLTVDRMISAVGVQGNIENLGLEALGVKTDRGCIVIDGYG
KTNVAGIYAIGDVAGPPMLAHKAEHEGVICVEKIAGLPNVHPLDMGKIPGCTYCNPQVASVGLTEAKAKEQGYDIRVGRY
SFSANGKAIALGEDQGLVKTIFDRKTGQLLGAHMVGAEVTELIQGFVIAMNLETTEEELMHSVFPHPTLSETMKESVLDA
YGRVLNA

Sequences:

>Translated_487_residues
MADIYDVIVIGSGPGGYVTAIRAAQLGLKTAIVEREHLGGICLNWGCIPTKALLRSAEILHFGEHAKDYGLKLDGTITPD
VKAVVQRSRGVSARLNGGVAFLMKKNKIDVIWGEAKLVKAASGSNPAEISVGKISKQPMQPQNPVPKGVLGEGSYKAKHI
IVATGARPRALPGIEPDGKLIWTYFEAMVPQALPKSMLVMGSGAIGIEFASFYHDMGVDVTVVEVMSQIMPVEDAETSAI
ARKQLEKRGLKIITDAKVAKVEKGANSVTAHVETKDGKTQTLTVDRMISAVGVQGNIENLGLEALGVKTDRGCIVIDGYG
KTNVAGIYAIGDVAGPPMLAHKAEHEGVICVEKIAGLPNVHPLDMGKIPGCTYCNPQVASVGLTEAKAKEQGYDIRVGRY
SFSANGKAIALGEDQGLVKTIFDRKTGQLLGAHMVGAEVTELIQGFVIAMNLETTEEELMHSVFPHPTLSETMKESVLDA
YGRVLNA
>Mature_486_residues
ADIYDVIVIGSGPGGYVTAIRAAQLGLKTAIVEREHLGGICLNWGCIPTKALLRSAEILHFGEHAKDYGLKLDGTITPDV
KAVVQRSRGVSARLNGGVAFLMKKNKIDVIWGEAKLVKAASGSNPAEISVGKISKQPMQPQNPVPKGVLGEGSYKAKHII
VATGARPRALPGIEPDGKLIWTYFEAMVPQALPKSMLVMGSGAIGIEFASFYHDMGVDVTVVEVMSQIMPVEDAETSAIA
RKQLEKRGLKIITDAKVAKVEKGANSVTAHVETKDGKTQTLTVDRMISAVGVQGNIENLGLEALGVKTDRGCIVIDGYGK
TNVAGIYAIGDVAGPPMLAHKAEHEGVICVEKIAGLPNVHPLDMGKIPGCTYCNPQVASVGLTEAKAKEQGYDIRVGRYS
FSANGKAIALGEDQGLVKTIFDRKTGQLLGAHMVGAEVTELIQGFVIAMNLETTEEELMHSVFPHPTLSETMKESVLDAY
GRVLNA

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=490, Percent_Identity=37.3469387755102, Blast_Score=281, Evalue=8e-76,
Organism=Homo sapiens, GI50301238, Length=487, Percent_Identity=27.9260780287474, Blast_Score=173, Evalue=3e-43,
Organism=Homo sapiens, GI22035672, Length=492, Percent_Identity=27.6422764227642, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI33519430, Length=478, Percent_Identity=25.1046025104602, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI33519428, Length=478, Percent_Identity=25.1046025104602, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI33519426, Length=478, Percent_Identity=25.1046025104602, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI148277065, Length=478, Percent_Identity=25.1046025104602, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI148277071, Length=478, Percent_Identity=25.1046025104602, Blast_Score=109, Evalue=7e-24,
Organism=Homo sapiens, GI291045266, Length=494, Percent_Identity=24.8987854251012, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI291045268, Length=490, Percent_Identity=24.6938775510204, Blast_Score=89, Evalue=1e-17,
Organism=Escherichia coli, GI1786307, Length=469, Percent_Identity=34.5415778251599, Blast_Score=258, Evalue=5e-70,
Organism=Escherichia coli, GI1789915, Length=465, Percent_Identity=30.752688172043, Blast_Score=169, Evalue=5e-43,
Organism=Escherichia coli, GI87082354, Length=490, Percent_Identity=26.9387755102041, Blast_Score=168, Evalue=9e-43,
Organism=Escherichia coli, GI87081717, Length=476, Percent_Identity=27.5210084033613, Blast_Score=155, Evalue=6e-39,
Organism=Caenorhabditis elegans, GI32565766, Length=488, Percent_Identity=36.8852459016393, Blast_Score=295, Evalue=4e-80,
Organism=Caenorhabditis elegans, GI71983429, Length=467, Percent_Identity=26.338329764454, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71983419, Length=467, Percent_Identity=26.338329764454, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17557007, Length=494, Percent_Identity=25.3036437246964, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71982272, Length=509, Percent_Identity=25.5402750491159, Blast_Score=92, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6321091, Length=489, Percent_Identity=37.2188139059305, Blast_Score=262, Evalue=9e-71,
Organism=Saccharomyces cerevisiae, GI6325240, Length=491, Percent_Identity=27.4949083503055, Blast_Score=166, Evalue=6e-42,
Organism=Saccharomyces cerevisiae, GI6325166, Length=486, Percent_Identity=29.0123456790123, Blast_Score=164, Evalue=2e-41,
Organism=Drosophila melanogaster, GI21358499, Length=490, Percent_Identity=38.9795918367347, Blast_Score=292, Evalue=3e-79,
Organism=Drosophila melanogaster, GI24640549, Length=489, Percent_Identity=26.9938650306748, Blast_Score=125, Evalue=8e-29,
Organism=Drosophila melanogaster, GI24640553, Length=489, Percent_Identity=26.9938650306748, Blast_Score=125, Evalue=9e-29,
Organism=Drosophila melanogaster, GI24640551, Length=489, Percent_Identity=26.9938650306748, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI17737741, Length=503, Percent_Identity=27.037773359841, Blast_Score=118, Evalue=8e-27,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 51740; Mature: 51608

Theoretical pI: Translated: 7.36; Mature: 7.36

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADIYDVIVIGSGPGGYVTAIRAAQLGLKTAIVEREHLGGICLNWGCIPTKALLRSAEIL
CCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHH
HFGEHAKDYGLKLDGTITPDVKAVVQRSRGVSARLNGGVAFLMKKNKIDVIWGEAKLVKA
CCCCCHHHCCEEECCCCCCHHHHHHHHHCCCEEEECCCEEEEEECCCEEEEECCEEEEEE
ASGSNPAEISVGKISKQPMQPQNPVPKGVLGEGSYKAKHIIVATGARPRALPGIEPDGKL
CCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCE
IWTYFEAMVPQALPKSMLVMGSGAIGIEFASFYHDMGVDVTVVEVMSQIMPVEDAETSAI
EHHHHHHHHHHHCCCCEEEEECCCCEEHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHH
ARKQLEKRGLKIITDAKVAKVEKGANSVTAHVETKDGKTQTLTVDRMISAVGVQGNIENL
HHHHHHHCCCEEEECCHHHHHHCCCCCEEEEEECCCCCEEEEEHHHHHHHHCCCCCHHHC
GLEALGVKTDRGCIVIDGYGKTNVAGIYAIGDVAGPPMLAHKAEHEGVICVEKIAGLPNV
CEEEEEEECCCCEEEEECCCCCCCEEEEEECCCCCCCHHHCCCCCCCEEEEHHHCCCCCC
HPLDMGKIPGCTYCNPQVASVGLTEAKAKEQGYDIRVGRYSFSANGKAIALGEDQGLVKT
CCCCCCCCCCCCCCCCCHHCCCCHHHHHHHCCCEEEEEEEEECCCCCEEEEECCCCHHHH
IFDRKTGQLLGAHMVGAEVTELIQGFVIAMNLETTEEELMHSVFPHPTLSETMKESVLDA
HHCCCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHH
YGRVLNA
HHHHHCC
>Mature Secondary Structure 
ADIYDVIVIGSGPGGYVTAIRAAQLGLKTAIVEREHLGGICLNWGCIPTKALLRSAEIL
CCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHH
HFGEHAKDYGLKLDGTITPDVKAVVQRSRGVSARLNGGVAFLMKKNKIDVIWGEAKLVKA
CCCCCHHHCCEEECCCCCCHHHHHHHHHCCCEEEECCCEEEEEECCCEEEEECCEEEEEE
ASGSNPAEISVGKISKQPMQPQNPVPKGVLGEGSYKAKHIIVATGARPRALPGIEPDGKL
CCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCE
IWTYFEAMVPQALPKSMLVMGSGAIGIEFASFYHDMGVDVTVVEVMSQIMPVEDAETSAI
EHHHHHHHHHHHCCCCEEEEECCCCEEHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHH
ARKQLEKRGLKIITDAKVAKVEKGANSVTAHVETKDGKTQTLTVDRMISAVGVQGNIENL
HHHHHHHCCCEEEECCHHHHHHCCCCCEEEEEECCCCCEEEEEHHHHHHHHCCCCCHHHC
GLEALGVKTDRGCIVIDGYGKTNVAGIYAIGDVAGPPMLAHKAEHEGVICVEKIAGLPNV
CEEEEEEECCCCEEEEECCCCCCCEEEEEECCCCCCCHHHCCCCCCCEEEEHHHCCCCCC
HPLDMGKIPGCTYCNPQVASVGLTEAKAKEQGYDIRVGRYSFSANGKAIALGEDQGLVKT
CCCCCCCCCCCCCCCCCHHCCCCHHHHHHHCCCEEEEEEEEECCCCCEEEEECCCCHHHH
IFDRKTGQLLGAHMVGAEVTELIQGFVIAMNLETTEEELMHSVFPHPTLSETMKESVLDA
HHCCCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHH
YGRVLNA
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9515924 [H]