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The map label for this gene is ntrX [H]

Identifier: 225852616

GI number: 225852616

Start: 1109949

End: 1111310

Strand: Reverse

Name: ntrX [H]

Synonym: BMEA_A1160

Alternate gene names: 225852616

Gene position: 1111310-1109949 (Counterclockwise)

Preceding gene: 225852617

Following gene: 225852615

Centisome position: 52.28

GC content: 60.21

Gene sequence:

>1362_bases
ATGGCGGCCGATATTCTTGTTGTTGATGACGAAGTGGATATCCGGGATCTTGTTGCTGGTATTCTGAGCGATGAAGGCCA
TGAAACACGCACCGCTTTCGATGCGGATAGCGCGCTTGCGGCCATTAATGACCGCGCTCCGCGGCTGGTGTTTCTCGATA
TATGGCTTCAGGGCAGCCGGCTCGACGGTCTGGCACTGCTGGACGAGATCAAGAAGCAGCATCCTGAATTGCCGGTGGTG
ATGATTTCCGGTCATGGCAATATCGAAACCGCCGTCTCGGCCATTCGTCGCGGCGCCTATGATTTCATCGAAAAGCCGTT
CAAGGCCGACCGCCTGATCCTCGTGGCGGAACGGGCGCTGGAAACCTCCAAGCTCAAGCGCGAGGTTTCCGACCTTCGCA
AACGCACCGGCGACCAGTTGGAACTGGTCGGCACGTCGCTCGCCATGAACCAGTTGCGCCAGACCATCGAGCGCGTGGCC
CCCACCAACAGCCGCATCATGATTACCGGCCCTTCCGGTGCAGGCAAGGAACTTGTGGCGCGCGCAATCCATGCCCAGTC
GAGCCGTGCCAATGGGCCTTTCGTAACGGTGAACGCGGCGACGATCACGCCGGAACGCATGGAAATAGAGCTGTTCGGCA
CGGAGATGGACGGTGGCGAACGCAAGGTCGGCGCGCTGGAAGAGGCTCATGGCGGCATCCTCTATCTGGACGAAGTGGCC
GATATGCCGCGCGAAACCCAGAACAAGATTTTGCGCGTGCTGGTCGATCAGCAGTTTGAACGTGTCGGTGGCACCAAGCG
CGTGAAGGTTGATGTACGCATCATTTCCTCCACGGCGCAGAACCTCGAAGGCATGATCGCGGAGGGCACTTTCCGCGAAG
ACCTGTTCCATCGTCTTTCGGTTGTTCCGGTGCAGGTGCCGGCGCTGGCCGCGCGGCGCGAGGATATTCCCTCGCTGGTC
GAGTTTTTCATGAAGCAGATTGCCGAGCAGGCGGGCATCAAGCCGCGCAAGATCGGGCCGGATGCCATGGCCGTGTTGCA
GGCGCATAGCTGGCCGGGCAATCTTCGCCAGTTGCGCAACAATGTCGAACGCCTGATGATCCTGACGCGAGGCGACGACC
CGGATGAACTCGTGACGGCCGATCTTTTGCCCGCGGAAATCGGCGACACGCTGCCGCGCGCACCGACGGAATCGGACCAG
CACATCATGGCGCTGCCGCTGCGTGAAGCGCGCGAGCGTTTCGAGAAGGAATACCTGATCGCGCAGATCAACCGTTTTGG
CGGCAATATCTCGCGCACGGCTGAATTCGTCGGGATGGAACGGTCTGCGCTCCATCGCAAGCTGAAGTCTCTCGGCGTAT
AA

Upstream 100 bases:

>100_bases
GGCGCAATGATACGTATGGTCTTTCCCGAGGATCCCCCCATTCCATCCGATGCGGATTCGGGAAATGACGAAAAGCACAC
AGCTGGACAGGTGATTTGAT

Downstream 100 bases:

>100_bases
TATAGCAGGGCGAACGGAGCGGATATGCGGGTAATTGTTTGCGGAGCAGGGCAGGTCGGCTATGGTATTGCCGAACGGCT
GGCTGCCGAGGAAAACGATG

Product: nitrogen assimilation regulatory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MAADILVVDDEVDIRDLVAGILSDEGHETRTAFDADSALAAINDRAPRLVFLDIWLQGSRLDGLALLDEIKKQHPELPVV
MISGHGNIETAVSAIRRGAYDFIEKPFKADRLILVAERALETSKLKREVSDLRKRTGDQLELVGTSLAMNQLRQTIERVA
PTNSRIMITGPSGAGKELVARAIHAQSSRANGPFVTVNAATITPERMEIELFGTEMDGGERKVGALEEAHGGILYLDEVA
DMPRETQNKILRVLVDQQFERVGGTKRVKVDVRIISSTAQNLEGMIAEGTFREDLFHRLSVVPVQVPALAARREDIPSLV
EFFMKQIAEQAGIKPRKIGPDAMAVLQAHSWPGNLRQLRNNVERLMILTRGDDPDELVTADLLPAEIGDTLPRAPTESDQ
HIMALPLREARERFEKEYLIAQINRFGGNISRTAEFVGMERSALHRKLKSLGV

Sequences:

>Translated_453_residues
MAADILVVDDEVDIRDLVAGILSDEGHETRTAFDADSALAAINDRAPRLVFLDIWLQGSRLDGLALLDEIKKQHPELPVV
MISGHGNIETAVSAIRRGAYDFIEKPFKADRLILVAERALETSKLKREVSDLRKRTGDQLELVGTSLAMNQLRQTIERVA
PTNSRIMITGPSGAGKELVARAIHAQSSRANGPFVTVNAATITPERMEIELFGTEMDGGERKVGALEEAHGGILYLDEVA
DMPRETQNKILRVLVDQQFERVGGTKRVKVDVRIISSTAQNLEGMIAEGTFREDLFHRLSVVPVQVPALAARREDIPSLV
EFFMKQIAEQAGIKPRKIGPDAMAVLQAHSWPGNLRQLRNNVERLMILTRGDDPDELVTADLLPAEIGDTLPRAPTESDQ
HIMALPLREARERFEKEYLIAQINRFGGNISRTAEFVGMERSALHRKLKSLGV
>Mature_452_residues
AADILVVDDEVDIRDLVAGILSDEGHETRTAFDADSALAAINDRAPRLVFLDIWLQGSRLDGLALLDEIKKQHPELPVVM
ISGHGNIETAVSAIRRGAYDFIEKPFKADRLILVAERALETSKLKREVSDLRKRTGDQLELVGTSLAMNQLRQTIERVAP
TNSRIMITGPSGAGKELVARAIHAQSSRANGPFVTVNAATITPERMEIELFGTEMDGGERKVGALEEAHGGILYLDEVAD
MPRETQNKILRVLVDQQFERVGGTKRVKVDVRIISSTAQNLEGMIAEGTFREDLFHRLSVVPVQVPALAARREDIPSLVE
FFMKQIAEQAGIKPRKIGPDAMAVLQAHSWPGNLRQLRNNVERLMILTRGDDPDELVTADLLPAEIGDTLPRAPTESDQH
IMALPLREARERFEKEYLIAQINRFGGNISRTAEFVGMERSALHRKLKSLGV

Specific function: Member of the two-component regulatory system ntrY/ntrX involved in the activation of nitrogen assimilatory genes such as glnA. NtrX is probably phosphorylated by ntrY and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=461, Percent_Identity=36.4425162689805, Blast_Score=299, Evalue=2e-82,
Organism=Escherichia coli, GI1790299, Length=470, Percent_Identity=37.4468085106383, Blast_Score=299, Evalue=2e-82,
Organism=Escherichia coli, GI1790437, Length=452, Percent_Identity=36.9469026548673, Blast_Score=275, Evalue=6e-75,
Organism=Escherichia coli, GI1788905, Length=451, Percent_Identity=35.4767184035477, Blast_Score=239, Evalue=4e-64,
Organism=Escherichia coli, GI1789233, Length=320, Percent_Identity=38.4375, Blast_Score=206, Evalue=3e-54,
Organism=Escherichia coli, GI1789087, Length=366, Percent_Identity=34.6994535519126, Blast_Score=192, Evalue=5e-50,
Organism=Escherichia coli, GI87082117, Length=320, Percent_Identity=35, Blast_Score=188, Evalue=5e-49,
Organism=Escherichia coli, GI1786524, Length=328, Percent_Identity=37.5, Blast_Score=181, Evalue=7e-47,
Organism=Escherichia coli, GI1787583, Length=315, Percent_Identity=33.6507936507937, Blast_Score=178, Evalue=8e-46,
Organism=Escherichia coli, GI87082152, Length=321, Percent_Identity=35.202492211838, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI87081872, Length=323, Percent_Identity=34.3653250773994, Blast_Score=162, Evalue=4e-41,
Organism=Escherichia coli, GI87081858, Length=289, Percent_Identity=27.681660899654, Blast_Score=121, Evalue=8e-29,
Organism=Escherichia coli, GI1789828, Length=231, Percent_Identity=31.1688311688312, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1786599, Length=115, Percent_Identity=36.5217391304348, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 50156; Mature: 50025

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAADILVVDDEVDIRDLVAGILSDEGHETRTAFDADSALAAINDRAPRLVFLDIWLQGSR
CCCEEEEECCCCCHHHHHHHHHCCCCCCHHHHCCCCHHHHHHCCCCCEEEEEEEEEECCC
LDGLALLDEIKKQHPELPVVMISGHGNIETAVSAIRRGAYDFIEKPFKADRLILVAERAL
CCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCHHHHHHCCCCCCCEEEEHHHHH
ETSKLKREVSDLRKRTGDQLELVGTSLAMNQLRQTIERVAPTNSRIMITGPSGAGKELVA
HHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHH
RAIHAQSSRANGPFVTVNAATITPERMEIELFGTEMDGGERKVGALEEAHGGILYLDEVA
HHHHHCCCCCCCCEEEEEEEEECCCEEEEEEEECCCCCCCCHHCCHHHCCCCEEEEHHHH
DMPRETQNKILRVLVDQQFERVGGTKRVKVDVRIISSTAQNLEGMIAEGTFREDLFHRLS
CCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEHHHHHHHCCHHCCCCHHHHHHHHHH
VVPVQVPALAARREDIPSLVEFFMKQIAEQAGIKPRKIGPDAMAVLQAHSWPGNLRQLRN
CCEEECHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHH
NVERLMILTRGDDPDELVTADLLPAEIGDTLPRAPTESDQHIMALPLREARERFEKEYLI
HHHHEEEEECCCCHHHHHHHHCCHHHHCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHH
AQINRFGGNISRTAEFVGMERSALHRKLKSLGV
HHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
AADILVVDDEVDIRDLVAGILSDEGHETRTAFDADSALAAINDRAPRLVFLDIWLQGSR
CCEEEEECCCCCHHHHHHHHHCCCCCCHHHHCCCCHHHHHHCCCCCEEEEEEEEEECCC
LDGLALLDEIKKQHPELPVVMISGHGNIETAVSAIRRGAYDFIEKPFKADRLILVAERAL
CCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCHHHHHHCCCCCCCEEEEHHHHH
ETSKLKREVSDLRKRTGDQLELVGTSLAMNQLRQTIERVAPTNSRIMITGPSGAGKELVA
HHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHH
RAIHAQSSRANGPFVTVNAATITPERMEIELFGTEMDGGERKVGALEEAHGGILYLDEVA
HHHHHCCCCCCCCEEEEEEEEECCCEEEEEEEECCCCCCCCHHCCHHHCCCCEEEEHHHH
DMPRETQNKILRVLVDQQFERVGGTKRVKVDVRIISSTAQNLEGMIAEGTFREDLFHRLS
CCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEHHHHHHHCCHHCCCCHHHHHHHHHH
VVPVQVPALAARREDIPSLVEFFMKQIAEQAGIKPRKIGPDAMAVLQAHSWPGNLRQLRN
CCEEECHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHH
NVERLMILTRGDDPDELVTADLLPAEIGDTLPRAPTESDQHIMALPLREARERFEKEYLI
HHHHEEEEECCCCHHHHHHHHCCHHHHCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHH
AQINRFGGNISRTAEFVGMERSALHRKLKSLGV
HHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1661370 [H]