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The map label for this gene is queA [H]

Identifier: 225852594

GI number: 225852594

Start: 1085977

End: 1087068

Strand: Reverse

Name: queA [H]

Synonym: BMEA_A1137

Alternate gene names: 225852594

Gene position: 1087068-1085977 (Counterclockwise)

Preceding gene: 225852595

Following gene: 225852593

Centisome position: 51.14

GC content: 58.24

Gene sequence:

>1092_bases
ATGCGTGTTGATCTTTTCGATTTCGATCTGCCGGAGGAGCGCATTGCCTTGCGCCCGGTTGAGCCGCGCGATCATGCAAA
ACTGTTGCACGTTCGCCCCGGAGAGCCGTTTGAAGATCGACACGTCTATGATCTGTTCGACCTTTTGCAGCCGGGCGACG
CGCTTGTTTTCAATGATACCAAGGTGATACCGGCACAGCTTGAAGGAATGCGCGAGCGCACGGGCAATATTTCGCAGGTC
AGTGCAACGCTGCATATGCGTGTCGGCCCTGATCGCTGGAAGGCTTTCCTGCGCCCTGCCAAGCGCGTGAAGGAAGGCGA
CCGCATCCGCTTCGGCCATTCCGGAACAAGCTGCTTTCTCGGCACGCTCGATGCAACGGTAGCTGAAAAAGGGGATTCGG
GCGAGGCGCTTCTGGTTTTCGATCTGTCCGGTGCGGTGCTGGACGAGGCGATTGCTGCCGTCGGCCATATTCCGTTGCCA
CCCTATATTGCTTCAAAGCGCCCTGAAGACGAGCGTGACCGCAAGGACTACCAGACCGTCTATGCGCGGGAGGAAGGTGC
GGTTGCCGCACCGACGGCGGGGCTGCATTTCACGCCGGATCTTCTGGAAAAGATCAAGGCCAGGGGCATTGAGGAGCATT
TCGTAACCCTGCATGTGGGGGCGGGCACCTTTCTGCCGGTCAAGGCCGACGACACCGGCGACCACAAGATGCATGCGGAA
ATCGGCCATGTTTCGCAAAGGACGGCGAGCGCGCTCAATGCCGTGCATGAGCGGGGCGGGCGTATTATATGCGTGGGTAC
GACTTCGCTACGGCTTATCGAGAGCGCCACGGGTGAGGACGGTGTTGTGCGGCCATGGTCTGGCGCGACGGATATCTTCA
TTACGCCCGGCTATCGCTTCCGGGCTGTCGATCTGCTGATGACCAATTTTCATCTTCCGCGTTCCACCCTTTTCATGCTG
GTTTCGGCCTTCAGCGGTCTCGATACGATGCATGCTGCTTACAATTATGCGATTGCCGACGGCTACCGCTTCTATTCCTA
TGGGGACGCAAGCTTGCTTGAACGTATCGACCACGATAGACACTCGGCATGA

Upstream 100 bases:

>100_bases
ATCTCGGCTCCGGCTTCGGCCGGGGCCGACTTTATGCGTTGCGTTTCCAAACGCGATATTGCTTCGCGCTTTCGCTGGAA
ATGCCCATACGAGGTAAGTC

Downstream 100 bases:

>100_bases
CCACAGAAAATTTCGGATTCAAGGTTCTCGCGCGTGATGGCGCGGCTCGTCAGGGTGAGATTTCCATGCCGCGCGGCGTG
GTGCGCACGCCCGCCTTCAT

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MRVDLFDFDLPEERIALRPVEPRDHAKLLHVRPGEPFEDRHVYDLFDLLQPGDALVFNDTKVIPAQLEGMRERTGNISQV
SATLHMRVGPDRWKAFLRPAKRVKEGDRIRFGHSGTSCFLGTLDATVAEKGDSGEALLVFDLSGAVLDEAIAAVGHIPLP
PYIASKRPEDERDRKDYQTVYAREEGAVAAPTAGLHFTPDLLEKIKARGIEEHFVTLHVGAGTFLPVKADDTGDHKMHAE
IGHVSQRTASALNAVHERGGRIICVGTTSLRLIESATGEDGVVRPWSGATDIFITPGYRFRAVDLLMTNFHLPRSTLFML
VSAFSGLDTMHAAYNYAIADGYRFYSYGDASLLERIDHDRHSA

Sequences:

>Translated_363_residues
MRVDLFDFDLPEERIALRPVEPRDHAKLLHVRPGEPFEDRHVYDLFDLLQPGDALVFNDTKVIPAQLEGMRERTGNISQV
SATLHMRVGPDRWKAFLRPAKRVKEGDRIRFGHSGTSCFLGTLDATVAEKGDSGEALLVFDLSGAVLDEAIAAVGHIPLP
PYIASKRPEDERDRKDYQTVYAREEGAVAAPTAGLHFTPDLLEKIKARGIEEHFVTLHVGAGTFLPVKADDTGDHKMHAE
IGHVSQRTASALNAVHERGGRIICVGTTSLRLIESATGEDGVVRPWSGATDIFITPGYRFRAVDLLMTNFHLPRSTLFML
VSAFSGLDTMHAAYNYAIADGYRFYSYGDASLLERIDHDRHSA
>Mature_363_residues
MRVDLFDFDLPEERIALRPVEPRDHAKLLHVRPGEPFEDRHVYDLFDLLQPGDALVFNDTKVIPAQLEGMRERTGNISQV
SATLHMRVGPDRWKAFLRPAKRVKEGDRIRFGHSGTSCFLGTLDATVAEKGDSGEALLVFDLSGAVLDEAIAAVGHIPLP
PYIASKRPEDERDRKDYQTVYAREEGAVAAPTAGLHFTPDLLEKIKARGIEEHFVTLHVGAGTFLPVKADDTGDHKMHAE
IGHVSQRTASALNAVHERGGRIICVGTTSLRLIESATGEDGVVRPWSGATDIFITPGYRFRAVDLLMTNFHLPRSTLFML
VSAFSGLDTMHAAYNYAIADGYRFYSYGDASLLERIDHDRHSA

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=357, Percent_Identity=44.8179271708684, Blast_Score=268, Evalue=5e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 40225; Mature: 40225

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVDLFDFDLPEERIALRPVEPRDHAKLLHVRPGEPFEDRHVYDLFDLLQPGDALVFNDT
CEEEEECCCCCHHHEEECCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHCCCCEEEECCC
KVIPAQLEGMRERTGNISQVSATLHMRVGPDRWKAFLRPAKRVKEGDRIRFGHSGTSCFL
EECHHHHHHHHHHCCCCEEEEEEEEEEECHHHHHHHHHHHHHCCCCCEEEECCCCCEEEE
GTLDATVAEKGDSGEALLVFDLSGAVLDEAIAAVGHIPLPPYIASKRPEDERDRKDYQTV
EEHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
YAREEGAVAAPTAGLHFTPDLLEKIKARGIEEHFVTLHVGAGTFLPVKADDTGDHKMHAE
HCCCCCCEECCCCCCCCCHHHHHHHHHCCCCEEEEEEEECCCEEEEEECCCCCCCEEHHH
IGHVSQRTASALNAVHERGGRIICVGTTSLRLIESATGEDGVVRPWSGATDIFITPGYRF
HHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEECCCCCCCEEECCCCCCEEEECCCCCE
RAVDLLMTNFHLPRSTLFMLVSAFSGLDTMHAAYNYAIADGYRFYSYGDASLLERIDHDR
EEEEEHHHCCCCCHHHHHHHHHHHCCCHHHHHHHEEEEECCEEEEEECCHHHHHHHHHCC
HSA
CCC
>Mature Secondary Structure
MRVDLFDFDLPEERIALRPVEPRDHAKLLHVRPGEPFEDRHVYDLFDLLQPGDALVFNDT
CEEEEECCCCCHHHEEECCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHCCCCEEEECCC
KVIPAQLEGMRERTGNISQVSATLHMRVGPDRWKAFLRPAKRVKEGDRIRFGHSGTSCFL
EECHHHHHHHHHHCCCCEEEEEEEEEEECHHHHHHHHHHHHHCCCCCEEEECCCCCEEEE
GTLDATVAEKGDSGEALLVFDLSGAVLDEAIAAVGHIPLPPYIASKRPEDERDRKDYQTV
EEHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
YAREEGAVAAPTAGLHFTPDLLEKIKARGIEEHFVTLHVGAGTFLPVKADDTGDHKMHAE
HCCCCCCEECCCCCCCCCHHHHHHHHHCCCCEEEEEEEECCCEEEEEECCCCCCCEEHHH
IGHVSQRTASALNAVHERGGRIICVGTTSLRLIESATGEDGVVRPWSGATDIFITPGYRF
HHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEECCCCCCCEEECCCCCCEEEECCCCCE
RAVDLLMTNFHLPRSTLFMLVSAFSGLDTMHAAYNYAIADGYRFYSYGDASLLERIDHDR
EEEEEHHHCCCCCHHHHHHHHHHHCCCHHHHHHHEEEEECCEEEEEECCHHHHHHHHHCC
HSA
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA