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The map label for this gene is holB [H]

Identifier: 225852498

GI number: 225852498

Start: 975829

End: 976911

Strand: Direct

Name: holB [H]

Synonym: BMEA_A1034

Alternate gene names: 225852498

Gene position: 975829-976911 (Clockwise)

Preceding gene: 225852497

Following gene: 225852499

Centisome position: 45.91

GC content: 59.83

Gene sequence:

>1083_bases
ATGATCGAAGAGCTGGACATTCCCAAAACACATGATGCCATTGAAGGCGTCCCCGGACCCTCGGCCAGCGCCTATCTGGC
GGGGCATGACGAGATCGCGCGTTTTCTGGCGCAGGCCTATCATGAAGGCAGGATGCACCATGCCTTGCTGTTCGAGGGAC
CGCAGGGCATTGGCAAGGCAACGCTCGCCTTTCATCTGGCGGGGCATATGCTCTCTTTCGGCGACCGGAACGAGGCTCCC
GAAACGCTAATCGCGCCGGATTTTTCAAAACCGCTCTGGCGGCAGATCGCGGGTGGCATGCACCCGGCGGTGCTGCACAT
CACCCGCCCTTTCGACCAGAAGACCGGAAAATTCCGCACCGCAATCACCGTGGAGGAGATCCGTCGCGTCACGCATTTTC
TGACGCGTACGGCTTCCGATCACGCCTGGCGCATCGTTATTGTCGATCCGGCGGACGATATGAACCGCAATGCGGCCAAT
GCGCTTTTGAAAACGCTGGAAGAGCCGCCGGCGCGCGCGCTTTTCATCCTTATTTCGCATTCATCGGGACGTCTTTTGCC
GACAATCCGCTCGCGCTGCCAGAGCATATCGTTCAAGCCGCTCGACGGTGAAACGCTTGGCCGCGCACTGTCACATGCGG
GGTCGAAGGCGGGCATCAGTGCGGGCGACATAACGGAAGTGCTGTTGCAGCGCTCGGAAGGCAGCGTGCGAAAGGCGCTG
CTTCTGCTGGCGCATGGCGGGCTGGAAATTTCCGATACGGTGGATCAGATCATAGCAGGCAGCATATTCGATCTTCCCAA
AGCGCAGGCGCTCGGCGGCATATTGAACGGGCGGGAAGCCGAAGTGCAATATGAGCTTTTCCGCGATTACCTGATGGGGC
GTATCGCAGCCGAAGCGCGGCGCTACGCCGATAGCGGGCAATTGCGTGAAGCTGATCAATGGGCGCGCTTCTGGAGCGAC
CTCATTCGCGAAACGGTGGAGGCGGAAACCTACAATCTCGACCGCAGGCAGGCGGTTGTTATCCTTCTGGAAAAGACGCA
TCGCGCTTTTCGCACCGGTGTGCCGCCGCTCACATCGGCTTGA

Upstream 100 bases:

>100_bases
CGGCGCGCTGCAAGGTCATTGATGCCGACCGTTCTCAGGAAAAAATTGCCGATGAAATCCGCTCTGTCGTGGACACGATC
CTGACGGAAAAAGGGCTTTT

Downstream 100 bases:

>100_bases
ATGAAATCGCCAACCAAGGGTGGGTTTCAACAACGCGCATGGACGTGACATGAACGCCTACATGCGTTATGTCACCGCTG
ACGAATTTCTTCTCGCGCGC

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MIEELDIPKTHDAIEGVPGPSASAYLAGHDEIARFLAQAYHEGRMHHALLFEGPQGIGKATLAFHLAGHMLSFGDRNEAP
ETLIAPDFSKPLWRQIAGGMHPAVLHITRPFDQKTGKFRTAITVEEIRRVTHFLTRTASDHAWRIVIVDPADDMNRNAAN
ALLKTLEEPPARALFILISHSSGRLLPTIRSRCQSISFKPLDGETLGRALSHAGSKAGISAGDITEVLLQRSEGSVRKAL
LLLAHGGLEISDTVDQIIAGSIFDLPKAQALGGILNGREAEVQYELFRDYLMGRIAAEARRYADSGQLREADQWARFWSD
LIRETVEAETYNLDRRQAVVILLEKTHRAFRTGVPPLTSA

Sequences:

>Translated_360_residues
MIEELDIPKTHDAIEGVPGPSASAYLAGHDEIARFLAQAYHEGRMHHALLFEGPQGIGKATLAFHLAGHMLSFGDRNEAP
ETLIAPDFSKPLWRQIAGGMHPAVLHITRPFDQKTGKFRTAITVEEIRRVTHFLTRTASDHAWRIVIVDPADDMNRNAAN
ALLKTLEEPPARALFILISHSSGRLLPTIRSRCQSISFKPLDGETLGRALSHAGSKAGISAGDITEVLLQRSEGSVRKAL
LLLAHGGLEISDTVDQIIAGSIFDLPKAQALGGILNGREAEVQYELFRDYLMGRIAAEARRYADSGQLREADQWARFWSD
LIRETVEAETYNLDRRQAVVILLEKTHRAFRTGVPPLTSA
>Mature_360_residues
MIEELDIPKTHDAIEGVPGPSASAYLAGHDEIARFLAQAYHEGRMHHALLFEGPQGIGKATLAFHLAGHMLSFGDRNEAP
ETLIAPDFSKPLWRQIAGGMHPAVLHITRPFDQKTGKFRTAITVEEIRRVTHFLTRTASDHAWRIVIVDPADDMNRNAAN
ALLKTLEEPPARALFILISHSSGRLLPTIRSRCQSISFKPLDGETLGRALSHAGSKAGISAGDITEVLLQRSEGSVRKAL
LLLAHGGLEISDTVDQIIAGSIFDLPKAQALGGILNGREAEVQYELFRDYLMGRIAAEARRYADSGQLREADQWARFWSD
LIRETVEAETYNLDRRQAVVILLEKTHRAFRTGVPPLTSA

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786676, Length=258, Percent_Identity=29.4573643410853, Blast_Score=79, Evalue=4e-16,
Organism=Drosophila melanogaster, GI19921136, Length=228, Percent_Identity=24.5614035087719, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003959
- InterPro:   IPR004622 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 39681; Mature: 39681

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEELDIPKTHDAIEGVPGPSASAYLAGHDEIARFLAQAYHEGRMHHALLFEGPQGIGKA
CCCCCCCCCCHHHHCCCCCCCCCEEECCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHH
TLAFHLAGHMLSFGDRNEAPETLIAPDFSKPLWRQIAGGMHPAVLHITRPFDQKTGKFRT
HHHHHHHHHHHHCCCCCCCCCCEECCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCEEE
AITVEEIRRVTHFLTRTASDHAWRIVIVDPADDMNRNAANALLKTLEEPPARALFILISH
EEEHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEC
SSGRLLPTIRSRCQSISFKPLDGETLGRALSHAGSKAGISAGDITEVLLQRSEGSVRKAL
CCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHH
LLLAHGGLEISDTVDQIIAGSIFDLPKAQALGGILNGREAEVQYELFRDYLMGRIAAEAR
HHHHCCCCCHHHHHHHHHHCHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
RYADSGQLREADQWARFWSDLIRETVEAETYNLDRRQAVVILLEKTHRAFRTGVPPLTSA
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEEEEEHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MIEELDIPKTHDAIEGVPGPSASAYLAGHDEIARFLAQAYHEGRMHHALLFEGPQGIGKA
CCCCCCCCCCHHHHCCCCCCCCCEEECCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHH
TLAFHLAGHMLSFGDRNEAPETLIAPDFSKPLWRQIAGGMHPAVLHITRPFDQKTGKFRT
HHHHHHHHHHHHCCCCCCCCCCEECCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCEEE
AITVEEIRRVTHFLTRTASDHAWRIVIVDPADDMNRNAANALLKTLEEPPARALFILISH
EEEHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEC
SSGRLLPTIRSRCQSISFKPLDGETLGRALSHAGSKAGISAGDITEVLLQRSEGSVRKAL
CCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHH
LLLAHGGLEISDTVDQIIAGSIFDLPKAQALGGILNGREAEVQYELFRDYLMGRIAAEAR
HHHHCCCCCHHHHHHHHHHCHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
RYADSGQLREADQWARFWSDLIRETVEAETYNLDRRQAVVILLEKTHRAFRTGVPPLTSA
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEEEEEHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550441; 10984043 [H]