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The map label for this gene is dacD [H]

Identifier: 225852496

GI number: 225852496

Start: 973916

End: 975106

Strand: Direct

Name: dacD [H]

Synonym: BMEA_A1032

Alternate gene names: 225852496

Gene position: 973916-975106 (Clockwise)

Preceding gene: 225852495

Following gene: 225852497

Centisome position: 45.82

GC content: 58.19

Gene sequence:

>1191_bases
ATGGGGCTTTTTTCCAGAATCACAGCAATCAGGGCTGGCCTTCTGTTGGGAACGGTTTTTGCCGCGGTGCCGCCTGCATG
GGCTGCACCTGCGAATTCGTCCATTGTCACCAAAGCACCGCAAGCCTTGCTGATCGACGATCGCAGCGGAACGGTTCTTC
TTTCAAAGGGCGCCGATAAGCCGGTTCCACCGGCTTCGCTGGCAAAGCTGATGACGGCGGAAGTTGTTTTCGAGGCCCTG
GAAAAGGGCCGCACAACGCTTGAAACAGCCTATCCTGTCAGCGAATATGCGTGGCGCACAGGCGGCGCACCGTCCGGCAC
ATCGACCATGTTTGCGCGCATCAAATCGACGCCGACGGTTGCAGATCTTCTGCAAGGCATGATCGTCCAATCGGCAAATG
ACGGCGCAATCGTTCTGGCCGAGGGCCTTGCCGGTAGCGAACAGACCTTTGCCGGGGCAATGAACCAGCGCGCGAAGGAG
CTTGGCCTTTCCTCGTCGAAATTCGTGAATTCCACCGGCCTGCCGGCGGCGGGGCAGGTGGTGACACTGGAGGATTTGCT
AAATCTCGCTCGCCATATCCATGCGGCGCATCCCGAATATTATCGATATTATGAGCAGCCCGCCTACACCTGGAATAATA
TCACCCAGCGCAACCGCAATCCGCTGCTGCGGCTCGAAGTTGGCGCGGACGGCATGGGAACCGGATTTACCGAGGCTTCC
GGCTATGCCCTGGTTGGCTCGGCTGAACAGAACGGCCGGCGGCTTTTTCTGGCGATGAGCGGCCTTGCCAGTGTGAAGGA
ACGGGAGGAAGAAGCCCGCAAACTCATTCAATGGGGCATGACCGGCTTTGATGATATGCGCATTTTTGCGGCAAACGATA
AGGTCGGCGATGCGCAGGTCTTTGGCGGGCAATCCTCAACCTTGCCGCTGGTGGTGAAGAAGGATGTGGAACTGCTTTTG
CCGAAGGAGGCGCGTGAAAAGCTGAAGGCACGCATCATCTATGAGGGTCCGTTGCAAGCCCCCATCGCCGCGGGAACGGA
AGTTGGCAGGCTGCAATTTGAATTAAACGGCAGCGTTTTGCAGCAAGTGCCCGTTTTTGCGGCGCAATCCGTCGCGCAAG
GCAGCCTGCCGCAGCGGGCATTGGGATCCGTGGTCGAGTTTTCGACCGGCTGGCTCAGGAAATACCTTTGA

Upstream 100 bases:

>100_bases
ACATGGCGGTGAGGGCGAATTGATTTTCGCTGAAACCGTTGCGTAAACTATATGCAGGCGTTCTGTTTCGCGACGTTCAG
GTTCAGGCAACGGGATTTTC

Downstream 100 bases:

>100_bases
AGGCTCGACCTCGCGCTTAAAGGCCGATATTAAACAGTGTGAGGCCCATTCGGGCATGGATTCATTTGTTCGGATGCCAG
AGTGTCGGGATTGTTCATCA

Product: serine-type D-Ala-D-Ala carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 6B; PBP-6B [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MGLFSRITAIRAGLLLGTVFAAVPPAWAAPANSSIVTKAPQALLIDDRSGTVLLSKGADKPVPPASLAKLMTAEVVFEAL
EKGRTTLETAYPVSEYAWRTGGAPSGTSTMFARIKSTPTVADLLQGMIVQSANDGAIVLAEGLAGSEQTFAGAMNQRAKE
LGLSSSKFVNSTGLPAAGQVVTLEDLLNLARHIHAAHPEYYRYYEQPAYTWNNITQRNRNPLLRLEVGADGMGTGFTEAS
GYALVGSAEQNGRRLFLAMSGLASVKEREEEARKLIQWGMTGFDDMRIFAANDKVGDAQVFGGQSSTLPLVVKKDVELLL
PKEAREKLKARIIYEGPLQAPIAAGTEVGRLQFELNGSVLQQVPVFAAQSVAQGSLPQRALGSVVEFSTGWLRKYL

Sequences:

>Translated_396_residues
MGLFSRITAIRAGLLLGTVFAAVPPAWAAPANSSIVTKAPQALLIDDRSGTVLLSKGADKPVPPASLAKLMTAEVVFEAL
EKGRTTLETAYPVSEYAWRTGGAPSGTSTMFARIKSTPTVADLLQGMIVQSANDGAIVLAEGLAGSEQTFAGAMNQRAKE
LGLSSSKFVNSTGLPAAGQVVTLEDLLNLARHIHAAHPEYYRYYEQPAYTWNNITQRNRNPLLRLEVGADGMGTGFTEAS
GYALVGSAEQNGRRLFLAMSGLASVKEREEEARKLIQWGMTGFDDMRIFAANDKVGDAQVFGGQSSTLPLVVKKDVELLL
PKEAREKLKARIIYEGPLQAPIAAGTEVGRLQFELNGSVLQQVPVFAAQSVAQGSLPQRALGSVVEFSTGWLRKYL
>Mature_395_residues
GLFSRITAIRAGLLLGTVFAAVPPAWAAPANSSIVTKAPQALLIDDRSGTVLLSKGADKPVPPASLAKLMTAEVVFEALE
KGRTTLETAYPVSEYAWRTGGAPSGTSTMFARIKSTPTVADLLQGMIVQSANDGAIVLAEGLAGSEQTFAGAMNQRAKEL
GLSSSKFVNSTGLPAAGQVVTLEDLLNLARHIHAAHPEYYRYYEQPAYTWNNITQRNRNPLLRLEVGADGMGTGFTEASG
YALVGSAEQNGRRLFLAMSGLASVKEREEEARKLIQWGMTGFDDMRIFAANDKVGDAQVFGGQSSTLPLVVKKDVELLLP
KEAREKLKARIIYEGPLQAPIAAGTEVGRLQFELNGSVLQQVPVFAAQSVAQGSLPQRALGSVVEFSTGWLRKYL

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential). Note=N-terminal lies in the periplasmic space (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI87082022, Length=340, Percent_Identity=35.2941176470588, Blast_Score=199, Evalue=4e-52,
Organism=Escherichia coli, GI1787062, Length=398, Percent_Identity=33.6683417085427, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI1786851, Length=394, Percent_Identity=31.4720812182741, Blast_Score=185, Evalue=3e-48,
Organism=Escherichia coli, GI87082054, Length=256, Percent_Identity=27.34375, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 42340; Mature: 42209

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLFSRITAIRAGLLLGTVFAAVPPAWAAPANSSIVTKAPQALLIDDRSGTVLLSKGADK
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCEEEEECCCCEEEEECCCCC
PVPPASLAKLMTAEVVFEALEKGRTTLETAYPVSEYAWRTGGAPSGTSTMFARIKSTPTV
CCCHHHHHHHHHHHHHHHHHHCCCCEEHHCCCHHHHHHCCCCCCCCCHHHHHHHCCCCCH
ADLLQGMIVQSANDGAIVLAEGLAGSEQTFAGAMNQRAKELGLSSSKFVNSTGLPAAGQV
HHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCE
VTLEDLLNLARHIHAAHPEYYRYYEQPAYTWNNITQRNRNPLLRLEVGADGMGTGFTEAS
EEHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHCCCCCEEEEEECCCCCCCCCCCCC
GYALVGSAEQNGRRLFLAMSGLASVKEREEEARKLIQWGMTGFDDMRIFAANDKVGDAQV
CEEEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEE
FGGQSSTLPLVVKKDVELLLPKEAREKLKARIIYEGPLQAPIAAGTEVGRLQFELNGSVL
ECCCCCCCCEEEECCCCEECCHHHHHHHHEEEEEECCCCCCCCCCCCEEEEEEEECCHHH
QQVPVFAAQSVAQGSLPQRALGSVVEFSTGWLRKYL
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GLFSRITAIRAGLLLGTVFAAVPPAWAAPANSSIVTKAPQALLIDDRSGTVLLSKGADK
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCEEEEECCCCEEEEECCCCC
PVPPASLAKLMTAEVVFEALEKGRTTLETAYPVSEYAWRTGGAPSGTSTMFARIKSTPTV
CCCHHHHHHHHHHHHHHHHHHCCCCEEHHCCCHHHHHHCCCCCCCCCHHHHHHHCCCCCH
ADLLQGMIVQSANDGAIVLAEGLAGSEQTFAGAMNQRAKELGLSSSKFVNSTGLPAAGQV
HHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCE
VTLEDLLNLARHIHAAHPEYYRYYEQPAYTWNNITQRNRNPLLRLEVGADGMGTGFTEAS
EEHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHCCCCCEEEEEECCCCCCCCCCCCC
GYALVGSAEQNGRRLFLAMSGLASVKEREEEARKLIQWGMTGFDDMRIFAANDKVGDAQV
CEEEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEE
FGGQSSTLPLVVKKDVELLLPKEAREKLKARIIYEGPLQAPIAAGTEVGRLQFELNGSVL
ECCCCCCCCEEEECCCCEECCHHHHHHHHEEEEEECCCCCCCCCCCCEEEEEEEECCHHH
QQVPVFAAQSVAQGSLPQRALGSVVEFSTGWLRKYL
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7737516; 11677609; 7751291 [H]