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The map label for this gene is tgt [H]

Identifier: 225852482

GI number: 225852482

Start: 962639

End: 963247

Strand: Direct

Name: tgt [H]

Synonym: BMEA_A1014

Alternate gene names: 225852482

Gene position: 962639-963247 (Clockwise)

Preceding gene: 225852479

Following gene: 225852483

Centisome position: 45.29

GC content: 59.11

Gene sequence:

>609_bases
GTGGCATTTGGCGACCAGCCGGGCAAGGCCATGTTCGGCATCGTGCAGGGGGGCGATATCGCGCGTCTGCGCGAAAGATC
GGCAGAAGCGCTCAAGGCCATGGACCTCAAGGGCTATTCGGTTGGTGGGCTTGCGGTGGGCGAGCCGCAGGAAGTCATGC
TGGATATGCTGGAAGTGGTCTGCCCGATCCTGCCAACGGAAAAGCCGCGCTACCTGATGGGGGTCGGCACGCCAGATGAT
ATTTTGAAATCCGTGGCGCGCGGGATCGATATGTTCGATTGCGTGATGCCGACGCGCGCGGGGCGCCATGGCCTTGCTTT
CACCCGCTTCGGCAAGGTGAATTTGCGCAATGCGCGTCATGCGGAAGATCATCGCCCGCTCGATCCGCAATCCGATTGTC
CGGCATCGCGCGATTATAGCCGCGCCTATCTGCATCATCTGGTGAAGTCCGGCGAGGCGCTCGGTGCAATGCTGCTCACC
TGGAACAATCTTGCCTATTATCAATATCTCATGAAGGGCATTCGCGCGGCGATTGCCGATGGAAATTTCAGTGATTTTAC
CGCTGAAACCACGGAAGGCTGGGCAAGAGGCGATATGCCTGCGCTCTAG

Upstream 100 bases:

>100_bases
TCTGTACGTACACGTGAGGGTATTATGAACCGCCGTCGCTTCCTTTCCTTCCTCGGCCTTGCTCCTGTAGCTTTCGCTGC
GCTGGGCCGAACGTTGCAAG

Downstream 100 bases:

>100_bases
GGCCTGTCTGCATACAAGGATTCCCTTTTGCATGAAATTCTGATTCAAGGTTGTTGAAGGAGAACAGCCTTGAGCAGACG
AAGCCTTACAGATGAGCAAT

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 202; Mature: 201

Protein sequence:

>202_residues
MAFGDQPGKAMFGIVQGGDIARLRERSAEALKAMDLKGYSVGGLAVGEPQEVMLDMLEVVCPILPTEKPRYLMGVGTPDD
ILKSVARGIDMFDCVMPTRAGRHGLAFTRFGKVNLRNARHAEDHRPLDPQSDCPASRDYSRAYLHHLVKSGEALGAMLLT
WNNLAYYQYLMKGIRAAIADGNFSDFTAETTEGWARGDMPAL

Sequences:

>Translated_202_residues
MAFGDQPGKAMFGIVQGGDIARLRERSAEALKAMDLKGYSVGGLAVGEPQEVMLDMLEVVCPILPTEKPRYLMGVGTPDD
ILKSVARGIDMFDCVMPTRAGRHGLAFTRFGKVNLRNARHAEDHRPLDPQSDCPASRDYSRAYLHHLVKSGEALGAMLLT
WNNLAYYQYLMKGIRAAIADGNFSDFTAETTEGWARGDMPAL
>Mature_201_residues
AFGDQPGKAMFGIVQGGDIARLRERSAEALKAMDLKGYSVGGLAVGEPQEVMLDMLEVVCPILPTEKPRYLMGVGTPDDI
LKSVARGIDMFDCVMPTRAGRHGLAFTRFGKVNLRNARHAEDHRPLDPQSDCPASRDYSRAYLHHLVKSGEALGAMLLTW
NNLAYYQYLMKGIRAAIADGNFSDFTAETTEGWARGDMPAL

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=178, Percent_Identity=40.4494382022472, Blast_Score=144, Evalue=5e-35,
Organism=Homo sapiens, GI13375872, Length=221, Percent_Identity=25.7918552036199, Blast_Score=91, Evalue=6e-19,
Organism=Escherichia coli, GI1786607, Length=194, Percent_Identity=51.5463917525773, Blast_Score=199, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI17544680, Length=182, Percent_Identity=36.2637362637363, Blast_Score=125, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI25146502, Length=162, Percent_Identity=28.3950617283951, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19920510, Length=181, Percent_Identity=42.5414364640884, Blast_Score=155, Evalue=2e-38,
Organism=Drosophila melanogaster, GI21355311, Length=178, Percent_Identity=28.0898876404494, Blast_Score=71, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 22151; Mature: 22020

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFGDQPGKAMFGIVQGGDIARLRERSAEALKAMDLKGYSVGGLAVGEPQEVMLDMLEVV
CCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCEECCCHHHHHHHHHHHH
CPILPTEKPRYLMGVGTPDDILKSVARGIDMFDCVMPTRAGRHGLAFTRFGKVNLRNARH
HCCCCCCCCCEEEECCCHHHHHHHHHHCCCHHHHHCCCCCCCCCCEEEEECCEECCCCCC
AEDHRPLDPQSDCPASRDYSRAYLHHLVKSGEALGAMLLTWNNLAYYQYLMKGIRAAIAD
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
GNFSDFTAETTEGWARGDMPAL
CCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AFGDQPGKAMFGIVQGGDIARLRERSAEALKAMDLKGYSVGGLAVGEPQEVMLDMLEVV
CCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCEECCCHHHHHHHHHHHH
CPILPTEKPRYLMGVGTPDDILKSVARGIDMFDCVMPTRAGRHGLAFTRFGKVNLRNARH
HCCCCCCCCCEEEECCCHHHHHHHHHHCCCHHHHHCCCCCCCCCCEEEEECCEECCCCCC
AEDHRPLDPQSDCPASRDYSRAYLHHLVKSGEALGAMLLTWNNLAYYQYLMKGIRAAIAD
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
GNFSDFTAETTEGWARGDMPAL
CCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]