Click here to switch to the map view.

The map label for this gene is fumA [H]

Identifier: 225852466

GI number: 225852466

Start: 951119

End: 952738

Strand: Reverse

Name: fumA [H]

Synonym: BMEA_A0995

Alternate gene names: 225852466

Gene position: 952738-951119 (Counterclockwise)

Preceding gene: 225852469

Following gene: 225852465

Centisome position: 44.82

GC content: 58.46

Gene sequence:

>1620_bases
ATGGCAGAAGCAAGCACCGCCGATCTTTTCCCTCTTGGCGAAGACGACACCCCTTATCGCAAACTGACTTCGGATTATGT
GTCGGTGGACACGTTCAAGGGGCAGGAAGTCCTGACTGTGGACCCGGAAGGGCTTCGCCTGCTTTCAGAAGCAGCCTTTG
CCGATATCAACCATCTGCTGCGCCCCGGCCATCTGCAACAGCTTGCAAAGATTCTTCAGGATCCCGAAGCAACCGACAAT
GACCGCTTCGTCGCCTACGACCTTTTGAAGAACGCCAATATCGCAGCCGGCGGCGTGCTGCCCATGTGCCAGGATACCGG
CACGGCCATTATCATGGGCAAGAAGGGCCGCCGCGTCTGGACCGAAGGCGGCGATGCCGATGCGCTCGGCGCCGGTGTGC
TGGACGCCTATAACAAGAAGAACCTGCGTTATTCGCAGCTCGCCCCGCTTTCGATGTTCGAGGAAAAGAACACCAAGAAC
AACCTGCCCGCGCAGATCGATATCTATGAAGAAGGCGAAGACGCCTACAAGTTCCTGTTCGTTGCCAAGGGCGGCGGTTC
GGCGAACAAGACGTTTCTCTATCAGGGAACGCCGTCGCTTCTGACGCATGACCGCATGATTGATTTCCTCAAGGACAAAA
TCCTGAGCCTCGGCACAGCCGCCTGCCCGCCCTATCATCTGGCCATCGTCATTGGCGGCACTTCGGCGGAAATGAACCTG
AAGACGGTGAAGCTTGCTTCCACGCGCTATCTCGACAGCCTGCCGACCAAAGGCTCGGAAACCGGCCACGCCTTCCGCGA
CCTTGAAATGGAAGCAGAAATTCACAAGCTGACCCAGTCGCTCGGTGTCGGCGCGCAGTTCGGCGGCAAATATTTCTGCC
ATGACGTGCGCGTCATCCGCCTGCCCCGCCACGGCGCTTCGCTGCCGATCGGCATCGGCGTTTCCTGTTCGGCCGACCGT
CAGGCGAAGGGCAAGATCACCAAGGACGGCATTTTCCTTGAGCAGCTTGAAGCCAATCCGGCAAAATACATGCCGGATAT
CGACGAGGAAAAGCTGACCGCGCATGTGGTCAAGATCGACCTCAACCAGCCGATGTCGGAAATACTCAAGGAACTCTCGA
AGCATCCGGTCAAGACGCGTCTTTCGCTCTCCGGCCCGATCATCGTGGCGCGCGATCTGGCACATGCGAAAATCCGCGAA
CGGCTGGAAAACGGCGAGCCAATGCCGGATTACTTCAAGAACCACCCGATCTATTATGCGGGCCCCGCCAAGACGCCCGC
GGGCTATGCTTCCGGCTCCTTCGGGCCGACAACGGCAGGCCGCATGGATTCCTATGTGGACCAGTTCCAGTCTTTCGGCG
GCTCCATGGTCATGCTCGCCAAGGGCAACCGTTCGCGCCAGGTGCGCGAAGCCTGCGGCCAGCACGGGGGCTTCTATCTC
GGCTCCATCGGCGGGCCGGCCGCACGTCTTGCGCAAGACTGCATCAAAAAGGTGGAAGTCGTCGAGTACCCGGAACTTGG
CATGGAGGCCATCTGGCGCATTGAAGTCGAAGATTTCCCGGCTTTCATCGTTATCGATGACAAGGGCAATGACTTCTTCA
AGGAATTCAACCTGAGTTGA

Upstream 100 bases:

>100_bases
AAGCCCCGCGGAATATGCCTTGAAGCTCCAGCTTCCGGGTTCAGAGCGCATCGTGCATTCCAGTTTTCTGGCCGGTGCGG
CAAGCAAGCGAGGTACCACG

Downstream 100 bases:

>100_bases
CCGCTACAGAAAAGGCCGGGACCAGAACCCGGCCTTTTCTTTATTCCCCTCATAAGGCCTCTCCGGCGATTGCCGGTCAA
ATATCTGTTCTTACGCACAT

Product: hydro-lyase, Fe-S type, tartrate/fumarate subfamily subunit beta

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 539; Mature: 538

Protein sequence:

>539_residues
MAEASTADLFPLGEDDTPYRKLTSDYVSVDTFKGQEVLTVDPEGLRLLSEAAFADINHLLRPGHLQQLAKILQDPEATDN
DRFVAYDLLKNANIAAGGVLPMCQDTGTAIIMGKKGRRVWTEGGDADALGAGVLDAYNKKNLRYSQLAPLSMFEEKNTKN
NLPAQIDIYEEGEDAYKFLFVAKGGGSANKTFLYQGTPSLLTHDRMIDFLKDKILSLGTAACPPYHLAIVIGGTSAEMNL
KTVKLASTRYLDSLPTKGSETGHAFRDLEMEAEIHKLTQSLGVGAQFGGKYFCHDVRVIRLPRHGASLPIGIGVSCSADR
QAKGKITKDGIFLEQLEANPAKYMPDIDEEKLTAHVVKIDLNQPMSEILKELSKHPVKTRLSLSGPIIVARDLAHAKIRE
RLENGEPMPDYFKNHPIYYAGPAKTPAGYASGSFGPTTAGRMDSYVDQFQSFGGSMVMLAKGNRSRQVREACGQHGGFYL
GSIGGPAARLAQDCIKKVEVVEYPELGMEAIWRIEVEDFPAFIVIDDKGNDFFKEFNLS

Sequences:

>Translated_539_residues
MAEASTADLFPLGEDDTPYRKLTSDYVSVDTFKGQEVLTVDPEGLRLLSEAAFADINHLLRPGHLQQLAKILQDPEATDN
DRFVAYDLLKNANIAAGGVLPMCQDTGTAIIMGKKGRRVWTEGGDADALGAGVLDAYNKKNLRYSQLAPLSMFEEKNTKN
NLPAQIDIYEEGEDAYKFLFVAKGGGSANKTFLYQGTPSLLTHDRMIDFLKDKILSLGTAACPPYHLAIVIGGTSAEMNL
KTVKLASTRYLDSLPTKGSETGHAFRDLEMEAEIHKLTQSLGVGAQFGGKYFCHDVRVIRLPRHGASLPIGIGVSCSADR
QAKGKITKDGIFLEQLEANPAKYMPDIDEEKLTAHVVKIDLNQPMSEILKELSKHPVKTRLSLSGPIIVARDLAHAKIRE
RLENGEPMPDYFKNHPIYYAGPAKTPAGYASGSFGPTTAGRMDSYVDQFQSFGGSMVMLAKGNRSRQVREACGQHGGFYL
GSIGGPAARLAQDCIKKVEVVEYPELGMEAIWRIEVEDFPAFIVIDDKGNDFFKEFNLS
>Mature_538_residues
AEASTADLFPLGEDDTPYRKLTSDYVSVDTFKGQEVLTVDPEGLRLLSEAAFADINHLLRPGHLQQLAKILQDPEATDND
RFVAYDLLKNANIAAGGVLPMCQDTGTAIIMGKKGRRVWTEGGDADALGAGVLDAYNKKNLRYSQLAPLSMFEEKNTKNN
LPAQIDIYEEGEDAYKFLFVAKGGGSANKTFLYQGTPSLLTHDRMIDFLKDKILSLGTAACPPYHLAIVIGGTSAEMNLK
TVKLASTRYLDSLPTKGSETGHAFRDLEMEAEIHKLTQSLGVGAQFGGKYFCHDVRVIRLPRHGASLPIGIGVSCSADRQ
AKGKITKDGIFLEQLEANPAKYMPDIDEEKLTAHVVKIDLNQPMSEILKELSKHPVKTRLSLSGPIIVARDLAHAKIRER
LENGEPMPDYFKNHPIYYAGPAKTPAGYASGSFGPTTAGRMDSYVDQFQSFGGSMVMLAKGNRSRQVREACGQHGGFYLG
SIGGPAARLAQDCIKKVEVVEYPELGMEAIWRIEVEDFPAFIVIDDKGNDFFKEFNLS

Specific function: It functions as an aerobic enzyme in the citric acid cycle. It accounts for about 80% of the fumarase activity when the bacteria grows aerobically [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1787897, Length=530, Percent_Identity=66.0377358490566, Blast_Score=742, Evalue=0.0,
Organism=Escherichia coli, GI1790564, Length=527, Percent_Identity=64.8956356736243, Blast_Score=724, Evalue=0.0,
Organism=Escherichia coli, GI1789443, Length=159, Percent_Identity=30.188679245283, Blast_Score=75, Evalue=9e-15,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 59034; Mature: 58903

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEASTADLFPLGEDDTPYRKLTSDYVSVDTFKGQEVLTVDPEGLRLLSEAAFADINHLL
CCCCCCCCEEECCCCCCCHHHHHHHHEEEEEECCCEEEEECCHHHHHHHHHHHHHHHHHC
RPGHLQQLAKILQDPEATDNDRFVAYDLLKNANIAAGGVLPMCQDTGTAIIMGKKGRRVW
CCHHHHHHHHHHCCCCCCCCCCEEEEEHHCCCCCCCCCCCCCCCCCCCEEEEECCCCEEE
TEGGDADALGAGVLDAYNKKNLRYSQLAPLSMFEEKNTKNNLPAQIDIYEEGEDAYKFLF
CCCCCCCHHCHHHHHHHCCCCCCHHHCCCHHHHHHCCCCCCCCEEEEEEECCCCEEEEEE
VAKGGGSANKTFLYQGTPSLLTHDRMIDFLKDKILSLGTAACPPYHLAIVIGGTSAEMNL
EECCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCEE
KTVKLASTRYLDSLPTKGSETGHAFRDLEMEAEIHKLTQSLGVGAQFGGKYFCHDVRVIR
EEEEEEHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECEEEEE
LPRHGASLPIGIGVSCSADRQAKGKITKDGIFLEQLEANPAKYMPDIDEEKLTAHVVKID
ECCCCCCCCEECCCCCCCCCCCCCCCCCCCEEEEECCCCCHHCCCCCCCCCEEEEEEEEE
LNQPMSEILKELSKHPVKTRLSLSGPIIVARDLAHAKIRERLENGEPMPDYFKNHPIYYA
CCCCHHHHHHHHHCCCCEEEEECCCCEEEEHHHHHHHHHHHHHCCCCCCHHHCCCCEEEE
GPAKTPAGYASGSFGPTTAGRMDSYVDQFQSFGGSMVMLAKGNRSRQVREACGQHGGFYL
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCCCCEEE
GSIGGPAARLAQDCIKKVEVVEYPELGMEAIWRIEVEDFPAFIVIDDKGNDFFKEFNLS
ECCCCHHHHHHHHHHHHHEECCCCCCCCEEEEEEEECCCCEEEEEECCCCHHHHHCCCC
>Mature Secondary Structure 
AEASTADLFPLGEDDTPYRKLTSDYVSVDTFKGQEVLTVDPEGLRLLSEAAFADINHLL
CCCCCCCEEECCCCCCCHHHHHHHHEEEEEECCCEEEEECCHHHHHHHHHHHHHHHHHC
RPGHLQQLAKILQDPEATDNDRFVAYDLLKNANIAAGGVLPMCQDTGTAIIMGKKGRRVW
CCHHHHHHHHHHCCCCCCCCCCEEEEEHHCCCCCCCCCCCCCCCCCCCEEEEECCCCEEE
TEGGDADALGAGVLDAYNKKNLRYSQLAPLSMFEEKNTKNNLPAQIDIYEEGEDAYKFLF
CCCCCCCHHCHHHHHHHCCCCCCHHHCCCHHHHHHCCCCCCCCEEEEEEECCCCEEEEEE
VAKGGGSANKTFLYQGTPSLLTHDRMIDFLKDKILSLGTAACPPYHLAIVIGGTSAEMNL
EECCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCEE
KTVKLASTRYLDSLPTKGSETGHAFRDLEMEAEIHKLTQSLGVGAQFGGKYFCHDVRVIR
EEEEEEHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECEEEEE
LPRHGASLPIGIGVSCSADRQAKGKITKDGIFLEQLEANPAKYMPDIDEEKLTAHVVKID
ECCCCCCCCEECCCCCCCCCCCCCCCCCCCEEEEECCCCCHHCCCCCCCCCEEEEEEEEE
LNQPMSEILKELSKHPVKTRLSLSGPIIVARDLAHAKIRERLENGEPMPDYFKNHPIYYA
CCCCHHHHHHHHHCCCCEEEEECCCCEEEEHHHHHHHHHHHHHCCCCCCHHHCCCCEEEE
GPAKTPAGYASGSFGPTTAGRMDSYVDQFQSFGGSMVMLAKGNRSRQVREACGQHGGFYL
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCCCCEEE
GSIGGPAARLAQDCIKKVEVVEYPELGMEAIWRIEVEDFPAFIVIDDKGNDFFKEFNLS
ECCCCHHHHHHHHHHHHHEECCCCCCCCEEEEEEEECCCCEEEEEECCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609; 1879695 [H]