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The map label for this gene is glxR [C]

Identifier: 225852460

GI number: 225852460

Start: 944684

End: 945559

Strand: Reverse

Name: glxR [C]

Synonym: BMEA_A0989

Alternate gene names: 225852460

Gene position: 945559-944684 (Counterclockwise)

Preceding gene: 225852461

Following gene: 225852450

Centisome position: 44.48

GC content: 55.82

Gene sequence:

>876_bases
ATGACAAACGTGAAAGTGGCTTTTCTTGGTCTCGGTGTGATGGGTTATCCCATGGCGGGGCATCTCAAAGCCAAGGGCGG
GCACGATGTTACGGTCTATAATCGCACCGCAGCGAAGGCGGCCAAATGGGCCGAACAATTCGGCGGCAACTATGCCGCCA
CTCCGGCAGATGCCGTAAAAAACGCAGATTTCGTCTTTGCTTGTATCGGCAATGACGACGATCTGCGCTCCGTCACCATT
GGCGCAGATGGTGCCTTTTCCACCATGAAAAAGGACGCAATCTTTATCGACAACACCACCGCATCGGCTGAAGTGGCGCG
CGAACTTGATAAAGAAGCGCAGAAGCGCGGCTTTCATTTCATGGATGCGCCTGTATCGGGCGGACAGGCAGGTGCCGAAA
ACGGCGTATTGACGGTAATGGTTGGCGCATCCGAGCCAGTCTTCGAGCGTGCAAAGCCGGTCATTGAGGCTTATGCCCGC
TCTGTCGGCCTTATGGGGCCGGTTGGCTCAGGCCAGCTTGCGAAAATGGTGAACCAGATCTGCATTGCAGGTCTTGTGCA
GGGTCTTGCGGAAGGCATTCATTTCGGCAAGAAAGCGGGCCTCGATATCGAGAAGCTTATCGCGGTTATCTCCAAGGGCG
CGGCCGGGTCGTGGCAGATGGAAAACCGCTCCAGCACCATGAACCAGGGCAAATATGATTTCGGCTTTGCCGTTGACTGG
ATGCGCAAGGATCTGGGCATCTGCCTTGATGAAGCAAATCGCAATGGCGCGCTCTTGCCCGTAACTGCGCTGGTGGACCA
GTTTTACAAGGAAGTGCAGAAGATCGGCGGCGGGCGCTGGGATACATCTTCGCTTCTCGCCCGGCTTGAACAGTAA

Upstream 100 bases:

>100_bases
CCTCGAAGACTGGTATCATCGCCTGCAACAGCGTACGGCCTATCGCGCGGCGGTTATGACACCCCTCACCTGATATGAAT
AAATCCAGAGGAATGATGCA

Downstream 100 bases:

>100_bases
GCAATACCATTCCAAATCAAAAAAGCCCGGCATCGCTGCCGGGCTTTTGCATGAACATCCAAAGCTTAGAATTTCACCTT
GGCAGTGAGCGAAAGCGCAC

Product: 6-phosphogluconate dehydrogenase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MTNVKVAFLGLGVMGYPMAGHLKAKGGHDVTVYNRTAAKAAKWAEQFGGNYAATPADAVKNADFVFACIGNDDDLRSVTI
GADGAFSTMKKDAIFIDNTTASAEVARELDKEAQKRGFHFMDAPVSGGQAGAENGVLTVMVGASEPVFERAKPVIEAYAR
SVGLMGPVGSGQLAKMVNQICIAGLVQGLAEGIHFGKKAGLDIEKLIAVISKGAAGSWQMENRSSTMNQGKYDFGFAVDW
MRKDLGICLDEANRNGALLPVTALVDQFYKEVQKIGGGRWDTSSLLARLEQ

Sequences:

>Translated_291_residues
MTNVKVAFLGLGVMGYPMAGHLKAKGGHDVTVYNRTAAKAAKWAEQFGGNYAATPADAVKNADFVFACIGNDDDLRSVTI
GADGAFSTMKKDAIFIDNTTASAEVARELDKEAQKRGFHFMDAPVSGGQAGAENGVLTVMVGASEPVFERAKPVIEAYAR
SVGLMGPVGSGQLAKMVNQICIAGLVQGLAEGIHFGKKAGLDIEKLIAVISKGAAGSWQMENRSSTMNQGKYDFGFAVDW
MRKDLGICLDEANRNGALLPVTALVDQFYKEVQKIGGGRWDTSSLLARLEQ
>Mature_290_residues
TNVKVAFLGLGVMGYPMAGHLKAKGGHDVTVYNRTAAKAAKWAEQFGGNYAATPADAVKNADFVFACIGNDDDLRSVTIG
ADGAFSTMKKDAIFIDNTTASAEVARELDKEAQKRGFHFMDAPVSGGQAGAENGVLTVMVGASEPVFERAKPVIEAYARS
VGLMGPVGSGQLAKMVNQICIAGLVQGLAEGIHFGKKAGLDIEKLIAVISKGAAGSWQMENRSSTMNQGKYDFGFAVDWM
RKDLGICLDEANRNGALLPVTALVDQFYKEVQKIGGGRWDTSSLLARLEQ

Specific function: Glyoxylate catabolism. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=297, Percent_Identity=31.3131313131313, Blast_Score=139, Evalue=3e-33,
Organism=Homo sapiens, GI40556376, Length=289, Percent_Identity=27.3356401384083, Blast_Score=127, Evalue=1e-29,
Organism=Escherichia coli, GI1786719, Length=287, Percent_Identity=31.0104529616725, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI145693186, Length=288, Percent_Identity=29.8611111111111, Blast_Score=141, Evalue=4e-35,
Organism=Escherichia coli, GI1790315, Length=286, Percent_Identity=31.1188811188811, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1789092, Length=226, Percent_Identity=30.5309734513274, Blast_Score=92, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17557316, Length=274, Percent_Identity=28.4671532846715, Blast_Score=98, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24655230, Length=288, Percent_Identity=31.9444444444444, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI19922568, Length=288, Percent_Identity=31.9444444444444, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI28574115, Length=282, Percent_Identity=22.6950354609929, Blast_Score=106, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 30875; Mature: 30743

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNVKVAFLGLGVMGYPMAGHLKAKGGHDVTVYNRTAAKAAKWAEQFGGNYAATPADAVK
CCCEEEEEEECCHHCCCCCCCEECCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCHHHHC
NADFVFACIGNDDDLRSVTIGADGAFSTMKKDAIFIDNTTASAEVARELDKEAQKRGFHF
CCCEEEEEECCCCCCEEEEECCCCCHHHHCCCEEEEECCCHHHHHHHHHHHHHHHCCCEE
MDAPVSGGQAGAENGVLTVMVGASEPVFERAKPVIEAYARSVGLMGPVGSGQLAKMVNQI
EECCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
CIAGLVQGLAEGIHFGKKAGLDIEKLIAVISKGAAGSWQMENRSSTMNQGKYDFGFAVDW
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCEECCCCCHHCCCCCCCCHHHHH
MRKDLGICLDEANRNGALLPVTALVDQFYKEVQKIGGGRWDTSSLLARLEQ
HHHHHHHEEECCCCCCCEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCC
>Mature Secondary Structure 
TNVKVAFLGLGVMGYPMAGHLKAKGGHDVTVYNRTAAKAAKWAEQFGGNYAATPADAVK
CCEEEEEEECCHHCCCCCCCEECCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCHHHHC
NADFVFACIGNDDDLRSVTIGADGAFSTMKKDAIFIDNTTASAEVARELDKEAQKRGFHF
CCCEEEEEECCCCCCEEEEECCCCCHHHHCCCEEEEECCCHHHHHHHHHHHHHHHCCCEE
MDAPVSGGQAGAENGVLTVMVGASEPVFERAKPVIEAYARSVGLMGPVGSGQLAKMVNQI
EECCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
CIAGLVQGLAEGIHFGKKAGLDIEKLIAVISKGAAGSWQMENRSSTMNQGKYDFGFAVDW
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCEECCCCCHHCCCCCCCCHHHHH
MRKDLGICLDEANRNGALLPVTALVDQFYKEVQKIGGGRWDTSSLLARLEQ
HHHHHHHEEECCCCCCCEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA