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The map label for this gene is ppnK

Identifier: 225852448

GI number: 225852448

Start: 932153

End: 932926

Strand: Direct

Name: ppnK

Synonym: BMEA_A0976

Alternate gene names: 225852448

Gene position: 932153-932926 (Clockwise)

Preceding gene: 225852446

Following gene: 225852449

Centisome position: 43.85

GC content: 54.26

Gene sequence:

>774_bases
ATGAAAGACACATCGCTTGCGCTTCATTTTGTTTCATCTGGCACAAAGGAATCGCTATCGGCCCAGAAAGATCTGGTAGA
AAGATACGGCCATGTTGCAGCGGAAGACGCCGATATCATCGTCGCTCTTGGAGGCGATGGCACCATGCTTCAGGCACTGC
GCGATTTCATGAATACGGGCAAGCCGATCTATGGCATGAACCGTGGTTCTGTCGGCTTTTTGATGAATGAGTTCGTGATC
GAAAACCTGCCCGAGCGCATTCTGGCCGCGCAGATGGAAACGATCCGCCCGCTTGTCATGGTAGCGGAAACCGACGATGC
GCCGCCTGTCGAGGCGTTGGCGATCAATGAAGTTTCACTTTTCCGCCAGTCCTATCAGGCTGCCCGCATTCGCATCACCA
TTGATGGCAAGGTGCGCCTGCAGGAACTGGTATGCGATGGTGTGATGGTGGCGACGCCAGCCGGATCAACGGCCTATAAT
CTTTCCGCGCAAGGGCCTATCCTGCCACTGGAGGCGCCCTTGCTGGCGCTGACGCCGGTCAGCCCGTTTCGCCCGCGCCG
ATGGGGCGGCGCACTTCTGCCAAAACATGTGACTGTCAGAATGGATTTGCTCGAAACAGAAAAACGCCCTGTCAATGCTG
TAGCGGATAATAACGAAGTCAAATCGGTTACGTCCGTTACTGTACGCGAAGCGCCAAACAGCCAGGTTACGATCCTGTTC
GACAAAAATCATTCCTGGGATGAGCGAATTCTCACAGAACAATTCAGGCATTAG

Upstream 100 bases:

>100_bases
ATCACCATGTTGAGATTATGGCAACCATGTTTTGCATGTGATAGCCAATAGGCGGCGGGAAAAAATAGCGGCAGATTTGC
CAGAAACGAGACCTGAGAGC

Downstream 100 bases:

>100_bases
GTGTGGTGGATTTGAAGTTCCTGTCATTTGCGAGGCGTCCGCGTTGAGGAATTTCAAATGTCGGGACTAAATATCTGGTG
CAGTATGACTTGAATATTTT

Product: inorganic polyphosphate/ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MKDTSLALHFVSSGTKESLSAQKDLVERYGHVAAEDADIIVALGGDGTMLQALRDFMNTGKPIYGMNRGSVGFLMNEFVI
ENLPERILAAQMETIRPLVMVAETDDAPPVEALAINEVSLFRQSYQAARIRITIDGKVRLQELVCDGVMVATPAGSTAYN
LSAQGPILPLEAPLLALTPVSPFRPRRWGGALLPKHVTVRMDLLETEKRPVNAVADNNEVKSVTSVTVREAPNSQVTILF
DKNHSWDERILTEQFRH

Sequences:

>Translated_257_residues
MKDTSLALHFVSSGTKESLSAQKDLVERYGHVAAEDADIIVALGGDGTMLQALRDFMNTGKPIYGMNRGSVGFLMNEFVI
ENLPERILAAQMETIRPLVMVAETDDAPPVEALAINEVSLFRQSYQAARIRITIDGKVRLQELVCDGVMVATPAGSTAYN
LSAQGPILPLEAPLLALTPVSPFRPRRWGGALLPKHVTVRMDLLETEKRPVNAVADNNEVKSVTSVTVREAPNSQVTILF
DKNHSWDERILTEQFRH
>Mature_257_residues
MKDTSLALHFVSSGTKESLSAQKDLVERYGHVAAEDADIIVALGGDGTMLQALRDFMNTGKPIYGMNRGSVGFLMNEFVI
ENLPERILAAQMETIRPLVMVAETDDAPPVEALAINEVSLFRQSYQAARIRITIDGKVRLQELVCDGVMVATPAGSTAYN
LSAQGPILPLEAPLLALTPVSPFRPRRWGGALLPKHVTVRMDLLETEKRPVNAVADNNEVKSVTSVTVREAPNSQVTILF
DKNHSWDERILTEQFRH

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family

Homologues:

Organism=Escherichia coli, GI1788968, Length=156, Percent_Identity=32.6923076923077, Blast_Score=69, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6325068, Length=207, Percent_Identity=31.4009661835749, Blast_Score=79, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6320794, Length=256, Percent_Identity=28.90625, Blast_Score=71, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6322509, Length=204, Percent_Identity=31.3725490196078, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPNK_BRUME (Q8YGW9)

Other databases:

- EMBL:   AE008917
- PIR:   AF3381
- RefSeq:   NP_539953.1
- ProteinModelPortal:   Q8YGW9
- GeneID:   1196747
- GenomeReviews:   AE008917_GR
- KEGG:   bme:BMEI1036
- HOGENOM:   HBG294544
- OMA:   HRIAINE
- PhylomeDB:   Q8YGW9
- ProtClustDB:   PRK04761
- BioCyc:   BMEL224914:BMEI1036-MONOMER
- BRENDA:   2.7.1.23
- GO:   GO:0005737
- HAMAP:   MF_00361
- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504
- Gene3D:   G3DSA:2.60.200.30
- Gene3D:   G3DSA:3.40.50.10330
- PANTHER:   PTHR20275

Pfam domain/function: PF01513 NAD_kinase; SSF111331 ATP-NAD_kinase_PpnK-typ

EC number: =2.7.1.23

Molecular weight: Translated: 28262; Mature: 28262

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDTSLALHFVSSGTKESLSAQKDLVERYGHVAAEDADIIVALGGDGTMLQALRDFMNTG
CCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHCC
KPIYGMNRGSVGFLMNEFVIENLPERILAAQMETIRPLVMVAETDDAPPVEALAINEVSL
CCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCEEEHHHHHH
FRQSYQAARIRITIDGKVRLQELVCDGVMVATPAGSTAYNLSAQGPILPLEAPLLALTPV
HHHHHCEEEEEEEECCCEEEEHHHHCCEEEECCCCCCEEECCCCCCEEECCCCEEEECCC
SPFRPRRWGGALLPKHVTVRMDLLETEKRPVNAVADNNEVKSVTSVTVREAPNSQVTILF
CCCCCCCCCCCCCCCEEEEEEEHHHCCCCCCCEECCCCCHHHEEEEEEEECCCCEEEEEE
DKNHSWDERILTEQFRH
CCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MKDTSLALHFVSSGTKESLSAQKDLVERYGHVAAEDADIIVALGGDGTMLQALRDFMNTG
CCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHCC
KPIYGMNRGSVGFLMNEFVIENLPERILAAQMETIRPLVMVAETDDAPPVEALAINEVSL
CCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCEEEHHHHHH
FRQSYQAARIRITIDGKVRLQELVCDGVMVATPAGSTAYNLSAQGPILPLEAPLLALTPV
HHHHHCEEEEEEEECCCEEEEHHHHCCEEEECCCCCCEEECCCCCCEEECCCCEEEECCC
SPFRPRRWGGALLPKHVTVRMDLLETEKRPVNAVADNNEVKSVTSVTVREAPNSQVTILF
CCCCCCCCCCCCCCCEEEEEEEHHHCCCCCCCEECCCCCHHHEEEEEEEECCCCEEEEEE
DKNHSWDERILTEQFRH
CCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11756688