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The map label for this gene is nuoI [H]

Identifier: 225852326

GI number: 225852326

Start: 809025

End: 809516

Strand: Direct

Name: nuoI [H]

Synonym: BMEA_A0852

Alternate gene names: 225852326

Gene position: 809025-809516 (Clockwise)

Preceding gene: 225852325

Following gene: 225852327

Centisome position: 38.06

GC content: 58.94

Gene sequence:

>492_bases
ATGGCTTCAATCGCTCAAGCAGCCAAATCGCTCCTGTTGAAGGAATTCGCCAGCGCGTTCGCTCTTTCCATGCGCCAGTT
CTTCGCGCCCAAGGCGACGCTGAACTATCCGCATGAAAAGGGCCCGGTCTCTCCGCGCTTTCGTGGCGAACATGCGCTGC
GCCGTTATCCGAACGGTGAAGAACGCTGCATCGCCTGCAAGCTTTGCGAAGCGATCTGCCCGGCGCAGGCCATCACCATC
GAGGCCGGCCCGCGCCGCAACGACGGCACCCGCCGTACGGTGCGCTATGACATCGATATGGTGAAATGCATCTATTGCGG
CTTCTGTCAGGAAGCCTGCCCGGTTGACGCTATCGTCGAAGGTCCGAACTTCGAGTTCGCGACCGAGACGCGCGAAGAAC
TTTACTACGACAAGGACAAGCTCCTTGCCAATGGTGATCGTTGGGAACGTGAAATCGCGCGCAATATCGCGATGGATGCG
CCCTATCGCTGA

Upstream 100 bases:

>100_bases
GCGCCTTGGCTGGAAAGTGTTCCTGCCGATCTCGCTCTTCATGGTTGTCGCAACCGCGACCTTCCTCAAAGTCTTCGGTC
TGGCGTAAGGAGTAAAACTC

Downstream 100 bases:

>100_bases
TTGGGTTAGAGCATTTCCGGCAAAAGTGCGAAGCGGTTTTGCCTAGGACAATGCGACAAAGGGTTTCGGCATTGGATTGG
AAAAGTCTTTTTAATCCGAT

Product: NADH dehydrogenase subunit I

Products: NA

Alternate protein names: NADH dehydrogenase I subunit I; NDH-1 subunit I [H]

Number of amino acids: Translated: 163; Mature: 162

Protein sequence:

>163_residues
MASIAQAAKSLLLKEFASAFALSMRQFFAPKATLNYPHEKGPVSPRFRGEHALRRYPNGEERCIACKLCEAICPAQAITI
EAGPRRNDGTRRTVRYDIDMVKCIYCGFCQEACPVDAIVEGPNFEFATETREELYYDKDKLLANGDRWEREIARNIAMDA
PYR

Sequences:

>Translated_163_residues
MASIAQAAKSLLLKEFASAFALSMRQFFAPKATLNYPHEKGPVSPRFRGEHALRRYPNGEERCIACKLCEAICPAQAITI
EAGPRRNDGTRRTVRYDIDMVKCIYCGFCQEACPVDAIVEGPNFEFATETREELYYDKDKLLANGDRWEREIARNIAMDA
PYR
>Mature_162_residues
ASIAQAAKSLLLKEFASAFALSMRQFFAPKATLNYPHEKGPVSPRFRGEHALRRYPNGEERCIACKLCEAICPAQAITIE
AGPRRNDGTRRTVRYDIDMVKCIYCGFCQEACPVDAIVEGPNFEFATETREELYYDKDKLLANGDRWEREIARNIAMDAP
YR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI4505371, Length=164, Percent_Identity=71.3414634146341, Blast_Score=241, Evalue=2e-64,
Organism=Escherichia coli, GI1788617, Length=142, Percent_Identity=38.0281690140845, Blast_Score=103, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17555194, Length=154, Percent_Identity=68.8311688311688, Blast_Score=226, Evalue=4e-60,
Organism=Drosophila melanogaster, GI17864306, Length=158, Percent_Identity=71.5189873417722, Blast_Score=242, Evalue=1e-64,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285
- InterPro:   IPR010226 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 18519; Mature: 18388

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
4.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASIAQAAKSLLLKEFASAFALSMRQFFAPKATLNYPHEKGPVSPRFRGEHALRRYPNGE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCC
ERCIACKLCEAICPAQAITIEAGPRRNDGTRRTVRYDIDMVKCIYCGFCQEACPVDAIVE
HHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCEEEC
GPNFEFATETREELYYDKDKLLANGDRWEREIARNIAMDAPYR
CCCCCCHHHHHHHHHHCHHHHHCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ASIAQAAKSLLLKEFASAFALSMRQFFAPKATLNYPHEKGPVSPRFRGEHALRRYPNGE
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCC
ERCIACKLCEAICPAQAITIEAGPRRNDGTRRTVRYDIDMVKCIYCGFCQEACPVDAIVE
HHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCEEEC
GPNFEFATETREELYYDKDKLLANGDRWEREIARNIAMDAPYR
CCCCCCHHHHHHHHHHCHHHHHCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]