Click here to switch to the map view.

The map label for this gene is fixK [H]

Identifier: 225852175

GI number: 225852175

Start: 665058

End: 665813

Strand: Direct

Name: fixK [H]

Synonym: BMEA_A0691

Alternate gene names: 225852175

Gene position: 665058-665813 (Clockwise)

Preceding gene: 225852174

Following gene: 225852176

Centisome position: 31.29

GC content: 56.61

Gene sequence:

>756_bases
GTGGTTGTAGTGCCTTTCGCATTAGATCAGAATCAAAAGCTTGCCGCCCGTACGCCCTGCAATGTGTGCGAAGATTGCTG
CGTCCGCAGCATGGCTGTGTGTTCCGCACTCGACGACGGCGATCTTGCCGCCCTCGAAGCCATCATGACGTCGAAAAAGC
TCGATACCAATGAGATGCTGGTTGAGGAAGGCGAACCGAAGCTTCGGGTCTATAGCCTGACCTCCGGCATGTTGCGCATC
TATACGTCGCTGCCGGATGGTCGCCGCCAGATCGCGGGCTTCCTGTTCCCCGGCGATTTTCTGGGGCTGGCCGATGATGA
GGTTTATTCGCTATCGGCGGAAGCGGTCGTGCCATCGGCCCTGTGTGCCTTTTCGGCCAAGGAAATGGAGCGTTTGATGG
AGCGCTTTCCCAAGCTGAAGGAGCGGCTGTACCAGATGACACGTCTGGCGCTGCGCACCGCGCGCGATAACCAGCTTGTG
CTTGGTCGTTTGGCGCCGGTCGAGAAACTGGCAAGCTTCCTTCTGGTTTTATCCGCACGTGCGGAAAAGCGCGGCGAAAA
GCCTAATCCCGTCCACCTTTTGATGAACCGTACCGATATCGCGGATTATCTTGGCCTCACCATAGAAACGGTAAGCCGCA
GCTTCACCAAGTTGAAGACGCAGGGGCTGATACAATTGCGCGATGCGAACACAGTTGAAATTCTCTCCCGCCGTTCACTG
GCTGTCGTTGCTGGACTGGACCCGGATAATCTTTGA

Upstream 100 bases:

>100_bases
CCAACCCTGCGGCGAAATTGCGCTTTGCGATGCGCGTCGAGGTTTCCTAATTAAAGGGAAAAAGCAGCTATCTGCTACCC
CCGCAGTATAGGAATTCATC

Downstream 100 bases:

>100_bases
TCCATAAAAATAGTGGTCCATCCGTGTTTGATTTAAATCAATGAAATATAGCCGTAGCTCCCGTAAATCCTGACTATAGA
GCATTTTCGAGCCAAAATTG

Product: transcriptional activator protein fnrA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MVVVPFALDQNQKLAARTPCNVCEDCCVRSMAVCSALDDGDLAALEAIMTSKKLDTNEMLVEEGEPKLRVYSLTSGMLRI
YTSLPDGRRQIAGFLFPGDFLGLADDEVYSLSAEAVVPSALCAFSAKEMERLMERFPKLKERLYQMTRLALRTARDNQLV
LGRLAPVEKLASFLLVLSARAEKRGEKPNPVHLLMNRTDIADYLGLTIETVSRSFTKLKTQGLIQLRDANTVEILSRRSL
AVVAGLDPDNL

Sequences:

>Translated_251_residues
MVVVPFALDQNQKLAARTPCNVCEDCCVRSMAVCSALDDGDLAALEAIMTSKKLDTNEMLVEEGEPKLRVYSLTSGMLRI
YTSLPDGRRQIAGFLFPGDFLGLADDEVYSLSAEAVVPSALCAFSAKEMERLMERFPKLKERLYQMTRLALRTARDNQLV
LGRLAPVEKLASFLLVLSARAEKRGEKPNPVHLLMNRTDIADYLGLTIETVSRSFTKLKTQGLIQLRDANTVEILSRRSL
AVVAGLDPDNL
>Mature_251_residues
MVVVPFALDQNQKLAARTPCNVCEDCCVRSMAVCSALDDGDLAALEAIMTSKKLDTNEMLVEEGEPKLRVYSLTSGMLRI
YTSLPDGRRQIAGFLFPGDFLGLADDEVYSLSAEAVVPSALCAFSAKEMERLMERFPKLKERLYQMTRLALRTARDNQLV
LGRLAPVEKLASFLLVLSARAEKRGEKPNPVHLLMNRTDIADYLGLTIETVSRSFTKLKTQGLIQLRDANTVEILSRRSL
AVVAGLDPDNL

Specific function: B.japonicum has two fixLJ-dependent fixK homologs that are activators of the transcription of a group of genes involved in anaerobic processes such as denitrification and possibly nitrogen fixation. FixK may bind DNA at the FNR consensus binding site [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787595, Length=228, Percent_Identity=28.9473684210526, Blast_Score=108, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 27783; Mature: 27783

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVVPFALDQNQKLAARTPCNVCEDCCVRSMAVCSALDDGDLAALEAIMTSKKLDTNEML
CEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHH
VEEGEPKLRVYSLTSGMLRIYTSLPDGRRQIAGFLFPGDFLGLADDEVYSLSAEAVVPSA
HHCCCCCEEEEEEHHHHHHHHHCCCCHHHHHHHEECCCCCCCCCCCHHHCCCHHHHHHHH
LCAFSAKEMERLMERFPKLKERLYQMTRLALRTARDNQLVLGRLAPVEKLASFLLVLSAR
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHH
AEKRGEKPNPVHLLMNRTDIADYLGLTIETVSRSFTKLKTQGLIQLRDANTVEILSRRSL
HHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHCCCE
AVVAGLDPDNL
EEEECCCCCCC
>Mature Secondary Structure
MVVVPFALDQNQKLAARTPCNVCEDCCVRSMAVCSALDDGDLAALEAIMTSKKLDTNEML
CEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHH
VEEGEPKLRVYSLTSGMLRIYTSLPDGRRQIAGFLFPGDFLGLADDEVYSLSAEAVVPSA
HHCCCCCEEEEEEHHHHHHHHHCCCCHHHHHHHEECCCCCCCCCCCHHHCCCHHHHHHHH
LCAFSAKEMERLMERFPKLKERLYQMTRLALRTARDNQLVLGRLAPVEKLASFLLVLSAR
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHH
AEKRGEKPNPVHLLMNRTDIADYLGLTIETVSRSFTKLKTQGLIQLRDANTVEILSRRSL
HHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHCCCE
AVVAGLDPDNL
EEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1551834; 12597275 [H]