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The map label for this gene is yfcG [H]

Identifier: 225852151

GI number: 225852151

Start: 643626

End: 644336

Strand: Direct

Name: yfcG [H]

Synonym: BMEA_A0665

Alternate gene names: 225852151

Gene position: 643626-644336 (Clockwise)

Preceding gene: 225852150

Following gene: 225852154

Centisome position: 30.28

GC content: 54.71

Gene sequence:

>711_bases
ATGGCTGAACAGACCAAGCCTATTGAACTTTATTATTGGCCGACGCCGAATGGCTTCAAAATCAGCATCATGCTGGAGGA
ACTGGGCGTCCCTTACGCGGTGAAATATATCAATATCGGCAAGGGCGATCAGTTTGAACCAGGTTTCCTGAAAATTGCAC
CTAACAATCGTATGCCCGCCATCGTCGATTCGGAAGGGCCGGGCGGCGAACCGATCTCGGTGTTTGAATCCGGTGCGATC
CTGCAATATCTGGGCCGTAAATTCGGCAAGTTTTATCCGACTGACAAGCGCAAGCGCGTTACGGTGGAAGAATGGCTGAT
GTGGCAGATGGGCGGGCTTGGCCCGATGTCCGGCCAGGCTGGGCATTTCCGCATCTATGCGCCGGAGAAAATCCAGTATG
GCATCGACCGTTATACGAATGAGGTCAACCGCCTCTATGGCGTCCTGAACCGCCGCCTTGAAGGCCGCGATTACATCGCC
GACGAATATTCGATCGCGGATATGGCCTGCATCGGCTGGGTCAACGCCTACAAGAATTACGAACAGAATCTCGATGATTT
CGCAAATCTCAAGCGGTGGCATGAAACCATGAACGCCCGGCCTGCCGTGCAGCGCGGGTTGCTGGTTGGCAAGGAAGAGC
GTGAGCGCGCCGATGCCGCAGCCAATAAGGAAGAGGAGCAGAAAATTCTCTTCGGCCAGAAGGCGCGTTGA

Upstream 100 bases:

>100_bases
CAAAAATATTGATGCCGGGTTAACTTGTACCTGTTTTCTTCCGGCCTATGCTAACGCTTATTGGATTCATGGACAAAGCC
AAGAAAGCGGGAGAAGACCC

Downstream 100 bases:

>100_bases
TCGTATATGGTATCAAAAGGGCGGCTTGTGGCCGCCCTTTGCTGTTTCTGCGAGTGCTCTATTGCCGGGCAAGGCTTTCG
CTCTTGCAGATCAGGCCGCC

Product: glutathione S-transferase domain-containing protein

Products: HX; R-S-glutathione

Alternate protein names: GST-like protein yfcG [H]

Number of amino acids: Translated: 236; Mature: 235

Protein sequence:

>236_residues
MAEQTKPIELYYWPTPNGFKISIMLEELGVPYAVKYINIGKGDQFEPGFLKIAPNNRMPAIVDSEGPGGEPISVFESGAI
LQYLGRKFGKFYPTDKRKRVTVEEWLMWQMGGLGPMSGQAGHFRIYAPEKIQYGIDRYTNEVNRLYGVLNRRLEGRDYIA
DEYSIADMACIGWVNAYKNYEQNLDDFANLKRWHETMNARPAVQRGLLVGKEERERADAAANKEEEQKILFGQKAR

Sequences:

>Translated_236_residues
MAEQTKPIELYYWPTPNGFKISIMLEELGVPYAVKYINIGKGDQFEPGFLKIAPNNRMPAIVDSEGPGGEPISVFESGAI
LQYLGRKFGKFYPTDKRKRVTVEEWLMWQMGGLGPMSGQAGHFRIYAPEKIQYGIDRYTNEVNRLYGVLNRRLEGRDYIA
DEYSIADMACIGWVNAYKNYEQNLDDFANLKRWHETMNARPAVQRGLLVGKEERERADAAANKEEEQKILFGQKAR
>Mature_235_residues
AEQTKPIELYYWPTPNGFKISIMLEELGVPYAVKYINIGKGDQFEPGFLKIAPNNRMPAIVDSEGPGGEPISVFESGAIL
QYLGRKFGKFYPTDKRKRVTVEEWLMWQMGGLGPMSGQAGHFRIYAPEKIQYGIDRYTNEVNRLYGVLNRRLEGRDYIAD
EYSIADMACIGWVNAYKNYEQNLDDFANLKRWHETMNARPAVQRGLLVGKEERERADAAANKEEEQKILFGQKAR

Specific function: Has disulfide bond reductase activity (in vitro). Has low hydroperoxidase activity with cumene hydroperoxide. Has very low glutathione-S-transferase activity (in vitro) [H]

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1788640, Length=214, Percent_Identity=50, Blast_Score=213, Evalue=7e-57,
Organism=Escherichia coli, GI87082195, Length=247, Percent_Identity=39.6761133603239, Blast_Score=164, Evalue=4e-42,
Organism=Escherichia coli, GI87081789, Length=173, Percent_Identity=24.8554913294798, Blast_Score=63, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324100, Length=248, Percent_Identity=31.8548387096774, Blast_Score=125, Evalue=5e-30,
Organism=Drosophila melanogaster, GI17933730, Length=232, Percent_Identity=30.1724137931034, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI17864592, Length=195, Percent_Identity=29.7435897435897, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI28571670, Length=172, Percent_Identity=31.3953488372093, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI45549270, Length=192, Percent_Identity=30.2083333333333, Blast_Score=87, Evalue=8e-18,
Organism=Drosophila melanogaster, GI17864598, Length=198, Percent_Identity=29.7979797979798, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI85725204, Length=198, Percent_Identity=28.7878787878788, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI17737923, Length=198, Percent_Identity=28.7878787878788, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI17864594, Length=194, Percent_Identity=28.8659793814433, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI17864596, Length=189, Percent_Identity=28.042328042328, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI221378983, Length=195, Percent_Identity=27.6923076923077, Blast_Score=77, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24646259, Length=193, Percent_Identity=27.979274611399, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24646249, Length=194, Percent_Identity=28.3505154639175, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24646251, Length=187, Percent_Identity=23.5294117647059, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: 2.5.1.18

Molecular weight: Translated: 27057; Mature: 26926

Theoretical pI: Translated: 7.49; Mature: 7.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEQTKPIELYYWPTPNGFKISIMLEELGVPYAVKYINIGKGDQFEPGFLKIAPNNRMPA
CCCCCCCEEEEECCCCCCEEEEEEHHHCCCCEEEEEEECCCCCCCCCCEEEECCCCCCCE
IVDSEGPGGEPISVFESGAILQYLGRKFGKFYPTDKRKRVTVEEWLMWQMGGLGPMSGQA
EEECCCCCCCCHHHHCCCHHHHHHHHHHCCCCCCCCCCEECHHHHHHHHHCCCCCCCCCC
GHFRIYAPEKIQYGIDRYTNEVNRLYGVLNRRLEGRDYIADEYSIADMACIGWVNAYKNY
CEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
EQNLDDFANLKRWHETMNARPAVQRGLLVGKEERERADAAANKEEEQKILFGQKAR
HCCHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHCCCCCC
>Mature Secondary Structure 
AEQTKPIELYYWPTPNGFKISIMLEELGVPYAVKYINIGKGDQFEPGFLKIAPNNRMPA
CCCCCCEEEEECCCCCCEEEEEEHHHCCCCEEEEEEECCCCCCCCCCEEEECCCCCCCE
IVDSEGPGGEPISVFESGAILQYLGRKFGKFYPTDKRKRVTVEEWLMWQMGGLGPMSGQA
EEECCCCCCCCHHHHCCCHHHHHHHHHHCCCCCCCCCCEECHHHHHHHHHCCCCCCCCCC
GHFRIYAPEKIQYGIDRYTNEVNRLYGVLNRRLEGRDYIADEYSIADMACIGWVNAYKNY
CEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
EQNLDDFANLKRWHETMNARPAVQRGLLVGKEERERADAAANKEEEQKILFGQKAR
HCCHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RX; glutathione

Specific reaction: RX + glutathione = HX + R-S-glutathione

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]