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The map label for this gene is gatA [H]

Identifier: 225852103

GI number: 225852103

Start: 587420

End: 588757

Strand: Direct

Name: gatA [H]

Synonym: BMEA_A0613

Alternate gene names: 225852103

Gene position: 587420-588757 (Clockwise)

Preceding gene: 225852102

Following gene: 225852106

Centisome position: 27.63

GC content: 60.31

Gene sequence:

>1338_bases
ATGGTCGGGGTTCGGGATCGGTTGGAAGCCACCCTCGCACGGCTTGCGGCGCGGGCGGGTGAGGAGCACGTCTTCAGCAA
GATCTATGCGGAGCGTGCACGGGCGGAGGCCGATGCAGCAGATACGCGGCTGCGCGCAGGGCGGCCGCTTGGTCCGCTTG
ATGGCCGCATCGTTTCGATCAAGGATCTGTTCGATGTGGCGGGCGAGCCGACGCTTGCGGGTTCGGTGGTTCGCCACGAT
GCCCCGCCTGCGGGCTGCGATGCTCTCATCGTGCAGCGCCTGCGCAATGCCGGAGCGGTTATTATCGGCAAAACCCACAT
GACCGAGTTTGCCTTCACGCCGGTCGGCCTCAATCCGCATTACGGTGAGCCGGGCAATGCGATTGACCCGGCGCGCATTC
CCGGTGGCTCATCGTCGGGTGCCGCCGTTTCGGTTGCCGAAGGCACGAGCGAGATCGCCATCGGTTCGGATACTGGCGGT
TCGGTGCGCATTCCGGCGGCACTGAACGGGCTGGTCGGTTTCAAGCCCACCGCGCGGCGCATTCCGCTGGAAGGCGCCTT
TCCGCTGGCGCCGTCGCTTGATTCGGTCGGGCCGCTCACCAGAACCGTGGCTGATGCTATCCTGGCTGATGCGGTGATGG
CCGGGGAAAAGCCCATTCTGCCGGAGGTCTTGCCGGTGAATGGGCTGCGTATCGCCTTGCCGAAAGACTATCTGCTCGCG
GACATGGAGCCAGATGTCGCAGCTCATTTTGAAGCGTCTCTGGCCGCACTTGAAAAGGCCGGAGCAATCATTGCCGATCT
TGCGGTGGACGACCTCATCGGGCGGCTGAAAGAGGCAACCCGCATAGGTTCGATTGCCGGTATAGAGGCAAGCCATATTC
ATGCTTCCACATGGCTCGCCGATCTGGACGCCAATGTCGATATTCGGGTCAAGCGCCCGCTTTCGGTACGCATAAAGGTG
CCGCTTGAGGCCTATCATGCGCTCATGGAAACGCGAAAGGCACTGGCGCGTGAAATGGATGAACGCCTGAGTGGTTTCGA
TATGTTCGCAACGCCTGCAACACCTATTGTTGCGCCGACAATTGCTTCCGTCAGCCACGACGAGGAGGAATATGACCGGG
TGGAGAACCTGCTTTTGCGCGATACGCAGGTCGCAAACCAGTTCGACCTATGCAGCATCACCCTGCCCATGCCGGGCATG
AAATTGCCGACTGGCCTCATGCTGACCGCACGCAATGACTCGGACAAGAGGCTGCTTGCTGCAGCCTTGTCGATTGAAAA
ATTATTAGAGCATGATCCCGAAAAGTGTGAGCGGTTTTCGGAAAAAGACCATGCTTAA

Upstream 100 bases:

>100_bases
GAGTGTGTGAAGAGGGTCGCGATTGACACTCTTCAGGCGTGATTATATCGCTCGGGCAGCAATATCTGAGCCATATGGCG
GTTTGCGGGGAGAAAAATCA

Downstream 100 bases:

>100_bases
ACAAAGGCCTAGAGACGGCCTGACTTAAAGTCGCCGTGCTCTAGGTTGATTCTTTCGCCAGCTCGGCTTCGCGGTGGATG
CGCGCCACCATGGCGCGCAG

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MVGVRDRLEATLARLAARAGEEHVFSKIYAERARAEADAADTRLRAGRPLGPLDGRIVSIKDLFDVAGEPTLAGSVVRHD
APPAGCDALIVQRLRNAGAVIIGKTHMTEFAFTPVGLNPHYGEPGNAIDPARIPGGSSSGAAVSVAEGTSEIAIGSDTGG
SVRIPAALNGLVGFKPTARRIPLEGAFPLAPSLDSVGPLTRTVADAILADAVMAGEKPILPEVLPVNGLRIALPKDYLLA
DMEPDVAAHFEASLAALEKAGAIIADLAVDDLIGRLKEATRIGSIAGIEASHIHASTWLADLDANVDIRVKRPLSVRIKV
PLEAYHALMETRKALAREMDERLSGFDMFATPATPIVAPTIASVSHDEEEYDRVENLLLRDTQVANQFDLCSITLPMPGM
KLPTGLMLTARNDSDKRLLAAALSIEKLLEHDPEKCERFSEKDHA

Sequences:

>Translated_445_residues
MVGVRDRLEATLARLAARAGEEHVFSKIYAERARAEADAADTRLRAGRPLGPLDGRIVSIKDLFDVAGEPTLAGSVVRHD
APPAGCDALIVQRLRNAGAVIIGKTHMTEFAFTPVGLNPHYGEPGNAIDPARIPGGSSSGAAVSVAEGTSEIAIGSDTGG
SVRIPAALNGLVGFKPTARRIPLEGAFPLAPSLDSVGPLTRTVADAILADAVMAGEKPILPEVLPVNGLRIALPKDYLLA
DMEPDVAAHFEASLAALEKAGAIIADLAVDDLIGRLKEATRIGSIAGIEASHIHASTWLADLDANVDIRVKRPLSVRIKV
PLEAYHALMETRKALAREMDERLSGFDMFATPATPIVAPTIASVSHDEEEYDRVENLLLRDTQVANQFDLCSITLPMPGM
KLPTGLMLTARNDSDKRLLAAALSIEKLLEHDPEKCERFSEKDHA
>Mature_445_residues
MVGVRDRLEATLARLAARAGEEHVFSKIYAERARAEADAADTRLRAGRPLGPLDGRIVSIKDLFDVAGEPTLAGSVVRHD
APPAGCDALIVQRLRNAGAVIIGKTHMTEFAFTPVGLNPHYGEPGNAIDPARIPGGSSSGAAVSVAEGTSEIAIGSDTGG
SVRIPAALNGLVGFKPTARRIPLEGAFPLAPSLDSVGPLTRTVADAILADAVMAGEKPILPEVLPVNGLRIALPKDYLLA
DMEPDVAAHFEASLAALEKAGAIIADLAVDDLIGRLKEATRIGSIAGIEASHIHASTWLADLDANVDIRVKRPLSVRIKV
PLEAYHALMETRKALAREMDERLSGFDMFATPATPIVAPTIASVSHDEEEYDRVENLLLRDTQVANQFDLCSITLPMPGM
KLPTGLMLTARNDSDKRLLAAALSIEKLLEHDPEKCERFSEKDHA

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=470, Percent_Identity=27.8723404255319, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI195972892, Length=441, Percent_Identity=26.530612244898, Blast_Score=107, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17543272, Length=469, Percent_Identity=26.6524520255864, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17556264, Length=244, Percent_Identity=29.5081967213115, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17537465, Length=172, Percent_Identity=35.4651162790698, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17538252, Length=182, Percent_Identity=31.8681318681319, Blast_Score=78, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI71990152, Length=173, Percent_Identity=32.9479768786127, Blast_Score=75, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17538254, Length=202, Percent_Identity=30.1980198019802, Blast_Score=67, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6319685, Length=404, Percent_Identity=28.2178217821782, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6323950, Length=203, Percent_Identity=32.512315270936, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320448, Length=200, Percent_Identity=32.5, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24648113, Length=473, Percent_Identity=28.7526427061311, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24652985, Length=451, Percent_Identity=25.9423503325942, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI19922090, Length=451, Percent_Identity=25.9423503325942, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24652981, Length=451, Percent_Identity=25.9423503325942, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24652983, Length=451, Percent_Identity=25.9423503325942, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI45550774, Length=190, Percent_Identity=32.6315789473684, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24648435, Length=192, Percent_Identity=32.2916666666667, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24648437, Length=192, Percent_Identity=32.2916666666667, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24648441, Length=190, Percent_Identity=32.6315789473684, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24648439, Length=190, Percent_Identity=32.6315789473684, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24644968, Length=222, Percent_Identity=29.2792792792793, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21356731, Length=181, Percent_Identity=30.939226519337, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 47275; Mature: 47275

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVGVRDRLEATLARLAARAGEEHVFSKIYAERARAEADAADTRLRAGRPLGPLDGRIVSI
CCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEH
KDLFDVAGEPTLAGSVVRHDAPPAGCDALIVQRLRNAGAVIIGKTHMTEFAFTPVGLNPH
HHHHHCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHCCCEEEEECCCCCEEEEECCCCCCC
YGEPGNAIDPARIPGGSSSGAAVSVAEGTSEIAIGSDTGGSVRIPAALNGLVGFKPTARR
CCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEECCCCCCEECCHHHCCCCCCCCCCCC
IPLEGAFPLAPSLDSVGPLTRTVADAILADAVMAGEKPILPEVLPVNGLRIALPKDYLLA
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCEEE
DMEPDVAAHFEASLAALEKAGAIIADLAVDDLIGRLKEATRIGSIAGIEASHIHASTWLA
CCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHEEHHHHHE
DLDANVDIRVKRPLSVRIKVPLEAYHALMETRKALAREMDERLSGFDMFATPATPIVAPT
ECCCCEEEEEECCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHH
IASVSHDEEEYDRVENLLLRDTQVANQFDLCSITLPMPGMKLPTGLMLTARNDSDKRLLA
HHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEEEECCCCCHHHHH
AALSIEKLLEHDPEKCERFSEKDHA
HHHHHHHHHHCCHHHHHHHHHCCCC
>Mature Secondary Structure
MVGVRDRLEATLARLAARAGEEHVFSKIYAERARAEADAADTRLRAGRPLGPLDGRIVSI
CCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEH
KDLFDVAGEPTLAGSVVRHDAPPAGCDALIVQRLRNAGAVIIGKTHMTEFAFTPVGLNPH
HHHHHCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHCCCEEEEECCCCCEEEEECCCCCCC
YGEPGNAIDPARIPGGSSSGAAVSVAEGTSEIAIGSDTGGSVRIPAALNGLVGFKPTARR
CCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEECCCCCCEECCHHHCCCCCCCCCCCC
IPLEGAFPLAPSLDSVGPLTRTVADAILADAVMAGEKPILPEVLPVNGLRIALPKDYLLA
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCEEE
DMEPDVAAHFEASLAALEKAGAIIADLAVDDLIGRLKEATRIGSIAGIEASHIHASTWLA
CCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHEEHHHHHE
DLDANVDIRVKRPLSVRIKVPLEAYHALMETRKALAREMDERLSGFDMFATPATPIVAPT
ECCCCEEEEEECCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHH
IASVSHDEEEYDRVENLLLRDTQVANQFDLCSITLPMPGMKLPTGLMLTARNDSDKRLLA
HHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEEEECCCCCHHHHH
AALSIEKLLEHDPEKCERFSEKDHA
HHHHHHHHHHCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA