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The map label for this gene is pucM [C]

Identifier: 225852043

GI number: 225852043

Start: 528166

End: 528522

Strand: Direct

Name: pucM [C]

Synonym: BMEA_A0544

Alternate gene names: 225852043

Gene position: 528166-528522 (Clockwise)

Preceding gene: 225852042

Following gene: 225852044

Centisome position: 24.85

GC content: 58.26

Gene sequence:

>357_bases
ATGGGTAAGCTTTCCACACATGTTCTCGATACCGCGCACGGCACCCCGGCTGCGGCGATGCGCGTCGAACTTTATCGCAT
CGCCGCCAGCGGAACGCCGGAACTCCTGAAGCGCGTTGTCACCAATCTGGACGGGCGCACCGATGCGCCGCTTCTCTCTG
GCGACGAAATGCGCACGGGCATTTATGAGCTGCAATTCCACGTTGCCGAATATTTTGAAGGCCGTGGTGCGGAACTGGCC
CATGAACCCTTCCTGGATCTCATCCCGATCCGGTTTGGCATTGCCGATGAAGACGGTAATTATCACGTGCCGCTTCTCGT
CAGCCCATGGTCCTATTCCACCTATCGCGGTAGCTGA

Upstream 100 bases:

>100_bases
TTGTCGACCGCGCGGGCAGGGAAGATAATCTGGAGGAGTATTTTTTCTCCGATTACGCCTATCGTATCGAAACCACACAA
ACGGCTTGAACGGGGTTCCC

Downstream 100 bases:

>100_bases
ACCTAAAACTTATCCCCCTGTTCGGGAGCGGAGCTAAACTCCTCGCGTCGTTTATTGCGAGGAGCTTTACATGGACGAAA
CACCCAATCTGAAACTGCCC

Product: hydroxyisourate hydrolase

Products: NA

Alternate protein names: HIU hydrolase; HIUHase

Number of amino acids: Translated: 118; Mature: 117

Protein sequence:

>118_residues
MGKLSTHVLDTAHGTPAAAMRVELYRIAASGTPELLKRVVTNLDGRTDAPLLSGDEMRTGIYELQFHVAEYFEGRGAELA
HEPFLDLIPIRFGIADEDGNYHVPLLVSPWSYSTYRGS

Sequences:

>Translated_118_residues
MGKLSTHVLDTAHGTPAAAMRVELYRIAASGTPELLKRVVTNLDGRTDAPLLSGDEMRTGIYELQFHVAEYFEGRGAELA
HEPFLDLIPIRFGIADEDGNYHVPLLVSPWSYSTYRGS
>Mature_117_residues
GKLSTHVLDTAHGTPAAAMRVELYRIAASGTPELLKRVVTNLDGRTDAPLLSGDEMRTGIYELQFHVAEYFEGRGAELAH
EPFLDLIPIRFGIADEDGNYHVPLLVSPWSYSTYRGS

Specific function: Catalyzes the hydrolysis of 5-hydroxyisourate (HIU) to 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline (OHCU)

COG id: COG2351

COG function: function code R; Transthyretin-like protein

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transthyretin family. 5-hydroxyisourate hydrolase subfamily

Homologues:

Organism=Escherichia coli, GI1788281, Length=115, Percent_Identity=39.1304347826087, Blast_Score=70, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI115532922, Length=118, Percent_Identity=33.8983050847458, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI115532920, Length=118, Percent_Identity=33.8983050847458, Blast_Score=62, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24652571, Length=116, Percent_Identity=40.5172413793103, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIUH_BRUME (Q8YFU1)

Other databases:

- EMBL:   AE008917
- PIR:   AG3430
- RefSeq:   NP_540346.1
- ProteinModelPortal:   Q8YFU1
- SMR:   Q8YFU1
- GeneID:   1197140
- GenomeReviews:   AE008917_GR
- KEGG:   bme:BMEI1429
- HOGENOM:   HBG445217
- OMA:   AGISTHV
- PhylomeDB:   Q8YFU1
- ProtClustDB:   CLSK898431
- BioCyc:   BMEL224914:BMEI1429-MONOMER
- GO:   GO:0006810
- InterPro:   IPR014306
- InterPro:   IPR000895
- Gene3D:   G3DSA:2.60.40.180
- PRINTS:   PR00189
- TIGRFAMs:   TIGR02962

Pfam domain/function: PF00576 Transthyretin; SSF49472 Transthyretin

EC number: =3.5.2.17

Molecular weight: Translated: 13033; Mature: 12902

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00768 TRANSTHYRETIN_1; PS00769 TRANSTHYRETIN_2

Important sites: BINDING 7-7 BINDING 46-46 BINDING 115-115

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKLSTHVLDTAHGTPAAAMRVELYRIAASGTPELLKRVVTNLDGRTDAPLLSGDEMRTG
CCCCCCCEEECCCCCCHHHHHEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCHHCC
IYELQFHVAEYFEGRGAELAHEPFLDLIPIRFGIADEDGNYHVPLLVSPWSYSTYRGS
HHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEECCCCCEEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
GKLSTHVLDTAHGTPAAAMRVELYRIAASGTPELLKRVVTNLDGRTDAPLLSGDEMRTG
CCCCCCEEECCCCCCHHHHHEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCHHCC
IYELQFHVAEYFEGRGAELAHEPFLDLIPIRFGIADEDGNYHVPLLVSPWSYSTYRGS
HHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEECCCCCEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11756688