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The map label for this gene is ppdK [H]

Identifier: 225852036

GI number: 225852036

Start: 520359

End: 523022

Strand: Direct

Name: ppdK [H]

Synonym: BMEA_A0537

Alternate gene names: 225852036

Gene position: 520359-523022 (Clockwise)

Preceding gene: 225852034

Following gene: 225852037

Centisome position: 24.48

GC content: 60.77

Gene sequence:

>2664_bases
ATGGCAAAGTGGGTGTACACGTTCGGTGATGGCAAGGCGGAAGGGGCTGCGAGCGATCGCAACCTTCTCGGCGGCAAGGG
GGCGAACCTGGCTGAAATGAGCAGCCTTGGCCTGCCTGTGCCGCCCGGCTTTACGATTACCACCGAAGTTTGCACTTATT
ATTACAATAACGACCGCGTCTATCCGTCCGAACTGGATGCGCAGGTTCAGGCGGCGCTTGCGCATATTGCAACGCTTACC
GGTCGTAATTTCGGTGATGCGGAAAAACCGCTTCTGGTTTCGGTGCGCTCCGGCGCGCGCGCATCCATGCCGGGCATGAT
GGATACGGTTCTCAATCTCGGGCTCAATGACGAGACGGTGCAGGCCATTGCCCGCGAATCGGGCGACGAACGTTTCGCCT
ATGACAGCTATCGCCGCTTTATCCAGATGTATTCGGATGTGGTCCTGGGTGTCGATCATGGCTTCTTCGAGGAAATTCTC
GAAGACACCAAGGCGGATCTCGGTGTCGAGGTCGATACGGCGCTTTCCGCCGACGACTGGAAGAACGTGATCGGGCTTTA
CAAGGCAAAGGTCGAGGAAGAACTGGGCCAGCCTTTCCCGCAAGACCCGCGCGAGCAGCTTTGGGGCGCAATCGGCGCCG
TTTTCTCAAGCTGGATGAATGCACGCGCCATCACCTATCGCCGCCTGCACAACATCCCGGCCGCATGGGGCACGGCAGTC
AATGTACAGGCCATGGTCTTCGGCAATATGGGGGAAACCTCGGCTACGGGCGTTGCCTTCACGCGCAACCCATCGACAGG
CGAAAACAAGCTCTATGGCGAATTCCTCGTCAATGCCCAGGGCGAAGACGTGGTGGCCGGTATCCGTACGCCGCAAAACA
TCACCGAGGAAGCGCGCATCGCCGCCGGTTCCGACAAGCCCTCGCTTGAAAAGGTGATGCCGGAAGCCTTTGCCGAATTT
TTGAAAGTCGCAAATCGTCTGGAACAGCATTATCGCGACATGCAGGATCTGGAATTTACCATCGAGCGCGGCAAGCTGTG
GATGCTCCAGACGCGCTCCGGCAAGCGCACTGCGAGGGCTGCGCTGAAAATGGCCGTGGAAATGGCGGCGGAAGGGCTGA
TTAGCGAGGAAGAGGCGGTGTTGCGTATCGACCCCGCAGCCCTTGACCAGCTTCTGCATCCCACCATCGATCCGCGCGCC
GAGCGTCAGGTCGTGGGCATGGGCCTGCCAGCTTCGCCGGGCGCTGCAACTGGCGAGATCGTGTTCAGCTCCGAAGAGGC
CGAACAGGCCAAGGCGGAAGGCCGCAACGTTATTCTGGTCCGCATTGAAACGAGCCCCGAAGATATTCACGGCATGCACG
CTGCCGAAGGCATTTTGACCACGCGCGGCGGCATGACCAGCCATGCGGCGGTGGTTGCGCGCGGCATGGGCAAGCCCTGT
GTTTCCGGCGCCGGTTCGCTGCGCGTCGATTACCGCAATGGCACCATGCTGGCTGCTGGCCAGACCTTCAGGAAGGGCGA
TGTCATCACGATTGATGGCGCAAGCGGCCAGGTGCTGAAAGGCTCTGTCGCCATGTTGCAGCCGGAGCTTTCCGGCGATT
TCGGCAAGCTGATGGAATGGGCCGACCGTGCGCGCCGCATGAAGGTGCGCGCCAATGCCGAAACACCAGCCGATGCCCGC
ACTGCGCGGTCATTTGGTGCGGAAGGCATCGGCCTTTGCCGCACGGAGCATATGTTCTTCGATGGCAGTCGCATCGTCGC
CATGCGCGAGATGATTTTGTCCGATACCGAGGAAGGTCGGCGGCTGGCGCTTGGCAAGCTTCTGCCGATGCAGCGTTCGG
ATTTTGCCGAACTGTTCGAGATCATGAAGGGCCTGCCCGTCACGATCCGCCTGCTCGATCCGCCGCTGCATGAATTCCTG
CCGCATACGGATGAGGAAGTGGACGAAGTCGCCCGGTCCATGGGCGTCGATGCTGCGAAGCTGCGCGACCGCGCCGATGC
GCTGCATGAGTTCAATCCGATGCTTGGCCATCGCGGTTGCCGTCTGGCTGTTTCCTATCCGGAAATAGCCGAAATGCAGG
CGCGCGCTATTTTCGAAGCTGCCGTGGAAGCGGGCAAGAAGACCGGCGAGCCGGTGGTGCCGGAAGTGATGGTGCCGCTG
GTGAGCTTGAAGGCGGAGCTTGATTTCGTCAAGGCGCGCATCGATGCGGTGGCGAAGGAAGTCATGAGCGAGGCAGGCAT
CAAGATCGACTATATGGTCGGCACGATGATCGAGCTGCCGCGCGCGGCACTCCGCGCGGCCGAGATTGCCGAATCTGCGG
AATTCTTCTCCTTCGGCACCAACGACCTGACGCAGACGACCTTCGGTATTTCGCGCGATGATGCCGCCGGCTTCCTCACG
ACATACCAGAACCGCGTTGTGATCGAGCAGGATCCGTTCGTTTCGCTCGACGTGGACGGTGTGGGCGAACTGGTGCAGAT
CGCTGCCGAACGCGGACGCAAGACGCGCGAAAAGATCAAGCTTGGCATCTGCGGTGAACATGGCGGCGACCCGGCTTCCA
TCGCCTTCTGCGAAAAGACGGGTCTCGACTATGTGTCCTGCTCGCCTTTCCGTGTGCCGATCGCCCGCCTTGCGGCGGCG
CAGGCAGCCGTGCGCAAAGTGTAA

Upstream 100 bases:

>100_bases
CTCGGCTTCTGATCGCATAAGCAACGCAAGCAGGTTCAGGGAGTGCTTGCAGCAATTGCAGGTCTTTCGGGAACCTTGTC
CTGTGTCAAGGGAGGCCGGA

Downstream 100 bases:

>100_bases
ACCGCTGCCATAATTCAGCTTTACTGCCGGGTTAGTTAGCGACTGCTAATGCGTTTTCGAGCCAAAAGTGCGAAGCGCCG
ATGCGGGGAAATCAGTCCAC

Product: pyruvate phosphate dikinase

Products: NA

Alternate protein names: Pyruvate, orthophosphate dikinase [H]

Number of amino acids: Translated: 887; Mature: 886

Protein sequence:

>887_residues
MAKWVYTFGDGKAEGAASDRNLLGGKGANLAEMSSLGLPVPPGFTITTEVCTYYYNNDRVYPSELDAQVQAALAHIATLT
GRNFGDAEKPLLVSVRSGARASMPGMMDTVLNLGLNDETVQAIARESGDERFAYDSYRRFIQMYSDVVLGVDHGFFEEIL
EDTKADLGVEVDTALSADDWKNVIGLYKAKVEEELGQPFPQDPREQLWGAIGAVFSSWMNARAITYRRLHNIPAAWGTAV
NVQAMVFGNMGETSATGVAFTRNPSTGENKLYGEFLVNAQGEDVVAGIRTPQNITEEARIAAGSDKPSLEKVMPEAFAEF
LKVANRLEQHYRDMQDLEFTIERGKLWMLQTRSGKRTARAALKMAVEMAAEGLISEEEAVLRIDPAALDQLLHPTIDPRA
ERQVVGMGLPASPGAATGEIVFSSEEAEQAKAEGRNVILVRIETSPEDIHGMHAAEGILTTRGGMTSHAAVVARGMGKPC
VSGAGSLRVDYRNGTMLAAGQTFRKGDVITIDGASGQVLKGSVAMLQPELSGDFGKLMEWADRARRMKVRANAETPADAR
TARSFGAEGIGLCRTEHMFFDGSRIVAMREMILSDTEEGRRLALGKLLPMQRSDFAELFEIMKGLPVTIRLLDPPLHEFL
PHTDEEVDEVARSMGVDAAKLRDRADALHEFNPMLGHRGCRLAVSYPEIAEMQARAIFEAAVEAGKKTGEPVVPEVMVPL
VSLKAELDFVKARIDAVAKEVMSEAGIKIDYMVGTMIELPRAALRAAEIAESAEFFSFGTNDLTQTTFGISRDDAAGFLT
TYQNRVVIEQDPFVSLDVDGVGELVQIAAERGRKTREKIKLGICGEHGGDPASIAFCEKTGLDYVSCSPFRVPIARLAAA
QAAVRKV

Sequences:

>Translated_887_residues
MAKWVYTFGDGKAEGAASDRNLLGGKGANLAEMSSLGLPVPPGFTITTEVCTYYYNNDRVYPSELDAQVQAALAHIATLT
GRNFGDAEKPLLVSVRSGARASMPGMMDTVLNLGLNDETVQAIARESGDERFAYDSYRRFIQMYSDVVLGVDHGFFEEIL
EDTKADLGVEVDTALSADDWKNVIGLYKAKVEEELGQPFPQDPREQLWGAIGAVFSSWMNARAITYRRLHNIPAAWGTAV
NVQAMVFGNMGETSATGVAFTRNPSTGENKLYGEFLVNAQGEDVVAGIRTPQNITEEARIAAGSDKPSLEKVMPEAFAEF
LKVANRLEQHYRDMQDLEFTIERGKLWMLQTRSGKRTARAALKMAVEMAAEGLISEEEAVLRIDPAALDQLLHPTIDPRA
ERQVVGMGLPASPGAATGEIVFSSEEAEQAKAEGRNVILVRIETSPEDIHGMHAAEGILTTRGGMTSHAAVVARGMGKPC
VSGAGSLRVDYRNGTMLAAGQTFRKGDVITIDGASGQVLKGSVAMLQPELSGDFGKLMEWADRARRMKVRANAETPADAR
TARSFGAEGIGLCRTEHMFFDGSRIVAMREMILSDTEEGRRLALGKLLPMQRSDFAELFEIMKGLPVTIRLLDPPLHEFL
PHTDEEVDEVARSMGVDAAKLRDRADALHEFNPMLGHRGCRLAVSYPEIAEMQARAIFEAAVEAGKKTGEPVVPEVMVPL
VSLKAELDFVKARIDAVAKEVMSEAGIKIDYMVGTMIELPRAALRAAEIAESAEFFSFGTNDLTQTTFGISRDDAAGFLT
TYQNRVVIEQDPFVSLDVDGVGELVQIAAERGRKTREKIKLGICGEHGGDPASIAFCEKTGLDYVSCSPFRVPIARLAAA
QAAVRKV
>Mature_886_residues
AKWVYTFGDGKAEGAASDRNLLGGKGANLAEMSSLGLPVPPGFTITTEVCTYYYNNDRVYPSELDAQVQAALAHIATLTG
RNFGDAEKPLLVSVRSGARASMPGMMDTVLNLGLNDETVQAIARESGDERFAYDSYRRFIQMYSDVVLGVDHGFFEEILE
DTKADLGVEVDTALSADDWKNVIGLYKAKVEEELGQPFPQDPREQLWGAIGAVFSSWMNARAITYRRLHNIPAAWGTAVN
VQAMVFGNMGETSATGVAFTRNPSTGENKLYGEFLVNAQGEDVVAGIRTPQNITEEARIAAGSDKPSLEKVMPEAFAEFL
KVANRLEQHYRDMQDLEFTIERGKLWMLQTRSGKRTARAALKMAVEMAAEGLISEEEAVLRIDPAALDQLLHPTIDPRAE
RQVVGMGLPASPGAATGEIVFSSEEAEQAKAEGRNVILVRIETSPEDIHGMHAAEGILTTRGGMTSHAAVVARGMGKPCV
SGAGSLRVDYRNGTMLAAGQTFRKGDVITIDGASGQVLKGSVAMLQPELSGDFGKLMEWADRARRMKVRANAETPADART
ARSFGAEGIGLCRTEHMFFDGSRIVAMREMILSDTEEGRRLALGKLLPMQRSDFAELFEIMKGLPVTIRLLDPPLHEFLP
HTDEEVDEVARSMGVDAAKLRDRADALHEFNPMLGHRGCRLAVSYPEIAEMQARAIFEAAVEAGKKTGEPVVPEVMVPLV
SLKAELDFVKARIDAVAKEVMSEAGIKIDYMVGTMIELPRAALRAAEIAESAEFFSFGTNDLTQTTFGISRDDAAGFLTT
YQNRVVIEQDPFVSLDVDGVGELVQIAAERGRKTREKIKLGICGEHGGDPASIAFCEKTGLDYVSCSPFRVPIARLAAAQ
AAVRKV

Specific function: Catalyzes the reversible phosphorylation of pyruvate and phosphate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=455, Percent_Identity=25.4945054945055, Blast_Score=107, Evalue=5e-24,
Organism=Escherichia coli, GI1787994, Length=743, Percent_Identity=25.1682368775236, Blast_Score=101, Evalue=2e-22,
Organism=Escherichia coli, GI1789193, Length=426, Percent_Identity=24.8826291079812, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI48994992, Length=447, Percent_Identity=25.0559284116331, Blast_Score=94, Evalue=4e-20,
Organism=Escherichia coli, GI1788726, Length=431, Percent_Identity=24.3619489559165, Blast_Score=80, Evalue=5e-16,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR010121
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.1 [H]

Molecular weight: Translated: 96371; Mature: 96240

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKWVYTFGDGKAEGAASDRNLLGGKGANLAEMSSLGLPVPPGFTITTEVCTYYYNNDRV
CCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCEEEEEEEEEEECCCEE
YPSELDAQVQAALAHIATLTGRNFGDAEKPLLVSVRSGARASMPGMMDTVLNLGLNDETV
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCHHHH
QAIARESGDERFAYDSYRRFIQMYSDVVLGVDHGFFEEILEDTKADLGVEVDTALSADDW
HHHHHHCCCCCHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHCCEEECCCCCHHHH
KNVIGLYKAKVEEELGQPFPQDPREQLWGAIGAVFSSWMNARAITYRRLHNIPAAWGTAV
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCE
NVQAMVFGNMGETSATGVAFTRNPSTGENKLYGEFLVNAQGEDVVAGIRTPQNITEEARI
EEEEEEECCCCCCCCCCEEEECCCCCCCCCEEEEEEECCCCCCEEEECCCCCCHHHHHHE
AAGSDKPSLEKVMPEAFAEFLKVANRLEQHYRDMQDLEFTIERGKLWMLQTRSGKRTARA
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCEEEEEECCCCHHHHH
ALKMAVEMAAEGLISEEEAVLRIDPAALDQLLHPTIDPRAERQVVGMGLPASPGAATGEI
HHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCEE
VFSSEEAEQAKAEGRNVILVRIETSPEDIHGMHAAEGILTTRGGMTSHAAVVARGMGKPC
EECCCHHHHHHHCCCCEEEEEEECCCHHHCCHHHHCCCEEECCCCCHHHHHHHHCCCCCC
VSGAGSLRVDYRNGTMLAAGQTFRKGDVITIDGASGQVLKGSVAMLQPELSGDFGKLMEW
CCCCCCEEEEECCCEEEECCCCCCCCCEEEEECCCCCEEECCHHEECCCCCCCHHHHHHH
ADRARRMKVRANAETPADARTARSFGAEGIGLCRTEHMFFDGSRIVAMREMILSDTEEGR
HHHHHHEEEEECCCCCCHHHHHHHCCCCCCCEEEEEEEEECCCHHHHHHHHHHCCCCCCC
RLALGKLLPMQRSDFAELFEIMKGLPVTIRLLDPPLHEFLPHTDEEVDEVARSMGVDAAK
EEHHHHHCCCCCCCHHHHHHHHCCCCEEEEEECCCHHHHCCCCHHHHHHHHHHHCCCHHH
LRDRADALHEFNPMLGHRGCRLAVSYPEIAEMQARAIFEAAVEAGKKTGEPVVPEVMVPL
HHHHHHHHHHHCHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
VSLKAELDFVKARIDAVAKEVMSEAGIKIDYMVGTMIELPRAALRAAEIAESAEFFSFGT
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
NDLTQTTFGISRDDAAGFLTTYQNRVVIEQDPFVSLDVDGVGELVQIAAERGRKTREKIK
CCHHHHHCCCCCCCCCHHHEECCCEEEEECCCEEEEECCCHHHHHHHHHHHCCCCHHHEE
LGICGEHGGDPASIAFCEKTGLDYVSCSPFRVPIARLAAAQAAVRKV
EEECCCCCCCCCEEEEEHHCCCCCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AKWVYTFGDGKAEGAASDRNLLGGKGANLAEMSSLGLPVPPGFTITTEVCTYYYNNDRV
CCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCEEEEEEEEEEECCCEE
YPSELDAQVQAALAHIATLTGRNFGDAEKPLLVSVRSGARASMPGMMDTVLNLGLNDETV
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCHHHH
QAIARESGDERFAYDSYRRFIQMYSDVVLGVDHGFFEEILEDTKADLGVEVDTALSADDW
HHHHHHCCCCCHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHCCEEECCCCCHHHH
KNVIGLYKAKVEEELGQPFPQDPREQLWGAIGAVFSSWMNARAITYRRLHNIPAAWGTAV
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCE
NVQAMVFGNMGETSATGVAFTRNPSTGENKLYGEFLVNAQGEDVVAGIRTPQNITEEARI
EEEEEEECCCCCCCCCCEEEECCCCCCCCCEEEEEEECCCCCCEEEECCCCCCHHHHHHE
AAGSDKPSLEKVMPEAFAEFLKVANRLEQHYRDMQDLEFTIERGKLWMLQTRSGKRTARA
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCEEEEEECCCCHHHHH
ALKMAVEMAAEGLISEEEAVLRIDPAALDQLLHPTIDPRAERQVVGMGLPASPGAATGEI
HHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCEE
VFSSEEAEQAKAEGRNVILVRIETSPEDIHGMHAAEGILTTRGGMTSHAAVVARGMGKPC
EECCCHHHHHHHCCCCEEEEEEECCCHHHCCHHHHCCCEEECCCCCHHHHHHHHCCCCCC
VSGAGSLRVDYRNGTMLAAGQTFRKGDVITIDGASGQVLKGSVAMLQPELSGDFGKLMEW
CCCCCCEEEEECCCEEEECCCCCCCCCEEEEECCCCCEEECCHHEECCCCCCCHHHHHHH
ADRARRMKVRANAETPADARTARSFGAEGIGLCRTEHMFFDGSRIVAMREMILSDTEEGR
HHHHHHEEEEECCCCCCHHHHHHHCCCCCCCEEEEEEEEECCCHHHHHHHHHHCCCCCCC
RLALGKLLPMQRSDFAELFEIMKGLPVTIRLLDPPLHEFLPHTDEEVDEVARSMGVDAAK
EEHHHHHCCCCCCCHHHHHHHHCCCCEEEEEECCCHHHHCCCCHHHHHHHHHHHCCCHHH
LRDRADALHEFNPMLGHRGCRLAVSYPEIAEMQARAIFEAAVEAGKKTGEPVVPEVMVPL
HHHHHHHHHHHCHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
VSLKAELDFVKARIDAVAKEVMSEAGIKIDYMVGTMIELPRAALRAAEIAESAEFFSFGT
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
NDLTQTTFGISRDDAAGFLTTYQNRVVIEQDPFVSLDVDGVGELVQIAAERGRKTREKIK
CCHHHHHCCCCCCCCCHHHEECCCEEEEECCCEEEEECCCHHHHHHHHHHHCCCCHHHEE
LGICGEHGGDPASIAFCEKTGLDYVSCSPFRVPIARLAAAQAAVRKV
EEECCCCCCCCCEEEEEHHCCCCCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430; 9168612 [H]