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The map label for this gene is rnhA

Identifier: 225852013

GI number: 225852013

Start: 495444

End: 495908

Strand: Direct

Name: rnhA

Synonym: BMEA_A0513

Alternate gene names: 225852013

Gene position: 495444-495908 (Clockwise)

Preceding gene: 225852012

Following gene: 225852016

Centisome position: 23.31

GC content: 59.78

Gene sequence:

>465_bases
GTGAAGCGCATCGAGGCCTATACGGACGGGGCCTGTTCCGGCAATCCCGGTCCCGGCGGCTGGGGTGCATTGCTGCGGTG
GAACGGCAATGAAAAGGAACTGAAGGGCGGCGAGGCGGAAACGACCAATAACCGCATGGAACTGATGGCGGCGATTTCTG
CACTTTCGGCGCTGAAGGAGCCTTGCGAAGTCGATCTCTACACCGACTCCGTCTATGTGCGCGATGGTATTTCCGGCTGG
ATCGAAGGCTGGAAGCGCAATGGCTGGAAAACGGCGGCCAAGAAGCCGGTGAAGAATGCCGAACTCTGGCAGGCGCTGGA
TGAGGCGCGAAAAGCCCACAAGGTCACTTGGCATTGGATCAAGGGCCATGCCGGGCACCCTGAAAACGAACGTGCGGATG
AACTGGCCCGCGCTGGCATGGAGCCGTTCAAATATGCCGGGCACCGCACGTTGAAGGTGAAATAG

Upstream 100 bases:

>100_bases
TGGTGAAGAAAGACCCGATGGAATATGTGCGCCGGATGCGCTTTCACCGCCAGATCGAATCCGCTGCCGAATATGGCCTT
GAAATGCAGGGAGTTGCGGC

Downstream 100 bases:

>100_bases
TAAGGGCGGCTTTCGCCGCCCTTTCACTTTTAAAGCTGTGCGAGAAGGGCTGAGGCGGCAGACGTGACCGCCTGGCCCGG
CTGTTCTTCGATATTGAGAG

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H

Number of amino acids: Translated: 154; Mature: 154

Protein sequence:

>154_residues
MKRIEAYTDGACSGNPGPGGWGALLRWNGNEKELKGGEAETTNNRMELMAAISALSALKEPCEVDLYTDSVYVRDGISGW
IEGWKRNGWKTAAKKPVKNAELWQALDEARKAHKVTWHWIKGHAGHPENERADELARAGMEPFKYAGHRTLKVK

Sequences:

>Translated_154_residues
MKRIEAYTDGACSGNPGPGGWGALLRWNGNEKELKGGEAETTNNRMELMAAISALSALKEPCEVDLYTDSVYVRDGISGW
IEGWKRNGWKTAAKKPVKNAELWQALDEARKAHKVTWHWIKGHAGHPENERADELARAGMEPFKYAGHRTLKVK
>Mature_154_residues
MKRIEAYTDGACSGNPGPGGWGALLRWNGNEKELKGGEAETTNNRMELMAAISALSALKEPCEVDLYTDSVYVRDGISGW
IEGWKRNGWKTAAKKPVKNAELWQALDEARKAHKVTWHWIKGHAGHPENERADELARAGMEPFKYAGHRTLKVK

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain

Homologues:

Organism=Homo sapiens, GI21359816, Length=141, Percent_Identity=37.5886524822695, Blast_Score=87, Evalue=6e-18,
Organism=Escherichia coli, GI1786408, Length=143, Percent_Identity=65.7342657342657, Blast_Score=198, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI115532542, Length=143, Percent_Identity=38.4615384615385, Blast_Score=89, Evalue=8e-19,
Organism=Drosophila melanogaster, GI45552509, Length=151, Percent_Identity=33.7748344370861, Blast_Score=83, Evalue=8e-17,
Organism=Drosophila melanogaster, GI17137476, Length=151, Percent_Identity=33.7748344370861, Blast_Score=83, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH_BRUA2 (Q2YMI3)

Other databases:

- EMBL:   AM040264
- RefSeq:   YP_413958.1
- HSSP:   P0A7Y4
- ProteinModelPortal:   Q2YMI3
- SMR:   Q2YMI3
- STRING:   Q2YMI3
- GeneID:   3788906
- GenomeReviews:   AM040264_GR
- KEGG:   bmf:BAB1_0503
- eggNOG:   COG0328
- HOGENOM:   HBG742437
- OMA:   NGWRTAD
- ProtClustDB:   PRK00203
- BioCyc:   BMEL359391:BAB1_0503-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00042
- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156

Pfam domain/function: PF00075 RnaseH; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 17173; Mature: 17173

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRIEAYTDGACSGNPGPGGWGALLRWNGNEKELKGGEAETTNNRMELMAAISALSALKE
CCCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHCC
PCEVDLYTDSVYVRDGISGWIEGWKRNGWKTAAKKPVKNAELWQALDEARKAHKVTWHWI
CCEEEEEECCEEEECCHHHHHHHHHCCCCCCHHCCCCCHHHHHHHHHHHHHHHCEEEEEE
KGHAGHPENERADELARAGMEPFKYAGHRTLKVK
ECCCCCCCCHHHHHHHHCCCCCHHHCCCEEEEEC
>Mature Secondary Structure
MKRIEAYTDGACSGNPGPGGWGALLRWNGNEKELKGGEAETTNNRMELMAAISALSALKE
CCCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHCC
PCEVDLYTDSVYVRDGISGWIEGWKRNGWKTAAKKPVKNAELWQALDEARKAHKVTWHWI
CCEEEEEECCEEEECCHHHHHHHHHCCCCCCHHCCCCCHHHHHHHHHHHHHHHCEEEEEE
KGHAGHPENERADELARAGMEPFKYAGHRTLKVK
ECCCCCCCCHHHHHHHHCCCCCHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA