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The map label for this gene is ispH [H]

Identifier: 225852011

GI number: 225852011

Start: 493343

End: 494461

Strand: Direct

Name: ispH [H]

Synonym: BMEA_A0511

Alternate gene names: 225852011

Gene position: 493343-494461 (Clockwise)

Preceding gene: 225852010

Following gene: 225852012

Centisome position: 23.21

GC content: 57.82

Gene sequence:

>1119_bases
ATGGAAAAAAGCCGTAAATCGCATGACAGGTCCGTCCTGCGTTTTTTGCGTCATCTACTGGAAAACGAGTACGCCATAAT
GACCCAACATCGCCCCCCTCTGGAGATCCGCCTTTGCGGCCCGCGCGGCTTTTGCGCAGGTGTGGACCGGGCCATCCAGA
TTGTCGTCCTGGCGCTCAAAAAATATGGTGCGCCGGTTTATGTACGCCATGAGATCGTGCACAATCGTTATGTGGTCGAG
GGGCTTCAGGCCCGCGGCGCGATCTTCGTCGAGGAACTGGATGAAATCCCGGCGGCACACCGCAACCAGCCGGTGGTGTT
TTCCGCCCATGGTGTGCCAAAATCCGTGCCAGCGGACGCGGAGGCCAAGAACCTCTTTTATCTTGATGCGACATGCCCAC
TGGTTTCCAAGGTGCACAAACAGGCCATGCGCCACCAGCGCCTTGGCCGCCATGTCATCCTGATCGGCCATTCCGGCCAC
CCGGAAGTGATCGGCACGATGGGGCAATTGCCGGATGGCGCGGTCACGCTGATTGAAACCGTCGAGGATGCGCACACCTG
CCATTTCGATGATGAAGACAATCTTGGCTTCGTCACGCAGACCACGCTGTCGGTGGATGACACGGCGGGTATCATCAAGG
AATTGCAGGCGCGCTTTCCCAATCTGGCGGCTCCGGCGGCGGAATCGATCTGCTACGCCACCACCAACCGGCAGGATGCT
GTGCGTGCGGCGGCGCCGGGCTGCGATCTGTTTCTGATTGTCGGTGCACCGAATTCCTCCAATTCCAAGCGTCTGGTCGA
GGTGGCGGAAAAGGCGGGCGCGCGCATGTCCATGCTGGTGCAGCGCGCGGAGGATATAGAATGGGAGCAGATCGGCGATA
TTTCCGTCGTTGGCCTTTCGGCTGGCGCGTCTGCGCCCGAAATCATCGTGGATGAAATCATCGATGCCTTCAAAGCCCGC
TTCGATGTGAAGATCGAACTGGCGGAAACAACGGTTGAAACCGAAAATTTCCTTGTGAATCGCGAAATCCGCGATGTGGA
ATTGACGGTGAAGGATATGGCATTCGTCAATGGCGAGCACCGCGTGGTCGGCATTTCCAAACTTATGCAGGGGAAGTAA

Upstream 100 bases:

>100_bases
CCTCGCGCTTGCGCTGATCGGGGTTGTCGGCACATGGCTGTGGCGCTATCGAAAAAGCGGCAGGGGTTGACAACGGCCCC
GTTGCAGCCCAACTCCGTGC

Downstream 100 bases:

>100_bases
GGGACATGGCCGTCTATACGGATATTAACGAGATCGAGCTTGGCGCATTCCTGCGGCATTACGATATCGGCACGCTGACC
TCCTATAAGGGGATCGCGGA

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MEKSRKSHDRSVLRFLRHLLENEYAIMTQHRPPLEIRLCGPRGFCAGVDRAIQIVVLALKKYGAPVYVRHEIVHNRYVVE
GLQARGAIFVEELDEIPAAHRNQPVVFSAHGVPKSVPADAEAKNLFYLDATCPLVSKVHKQAMRHQRLGRHVILIGHSGH
PEVIGTMGQLPDGAVTLIETVEDAHTCHFDDEDNLGFVTQTTLSVDDTAGIIKELQARFPNLAAPAAESICYATTNRQDA
VRAAAPGCDLFLIVGAPNSSNSKRLVEVAEKAGARMSMLVQRAEDIEWEQIGDISVVGLSAGASAPEIIVDEIIDAFKAR
FDVKIELAETTVETENFLVNREIRDVELTVKDMAFVNGEHRVVGISKLMQGK

Sequences:

>Translated_372_residues
MEKSRKSHDRSVLRFLRHLLENEYAIMTQHRPPLEIRLCGPRGFCAGVDRAIQIVVLALKKYGAPVYVRHEIVHNRYVVE
GLQARGAIFVEELDEIPAAHRNQPVVFSAHGVPKSVPADAEAKNLFYLDATCPLVSKVHKQAMRHQRLGRHVILIGHSGH
PEVIGTMGQLPDGAVTLIETVEDAHTCHFDDEDNLGFVTQTTLSVDDTAGIIKELQARFPNLAAPAAESICYATTNRQDA
VRAAAPGCDLFLIVGAPNSSNSKRLVEVAEKAGARMSMLVQRAEDIEWEQIGDISVVGLSAGASAPEIIVDEIIDAFKAR
FDVKIELAETTVETENFLVNREIRDVELTVKDMAFVNGEHRVVGISKLMQGK
>Mature_372_residues
MEKSRKSHDRSVLRFLRHLLENEYAIMTQHRPPLEIRLCGPRGFCAGVDRAIQIVVLALKKYGAPVYVRHEIVHNRYVVE
GLQARGAIFVEELDEIPAAHRNQPVVFSAHGVPKSVPADAEAKNLFYLDATCPLVSKVHKQAMRHQRLGRHVILIGHSGH
PEVIGTMGQLPDGAVTLIETVEDAHTCHFDDEDNLGFVTQTTLSVDDTAGIIKELQARFPNLAAPAAESICYATTNRQDA
VRAAAPGCDLFLIVGAPNSSNSKRLVEVAEKAGARMSMLVQRAEDIEWEQIGDISVVGLSAGASAPEIIVDEIIDAFKAR
FDVKIELAETTVETENFLVNREIRDVELTVKDMAFVNGEHRVVGISKLMQGK

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) [H]

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family [H]

Homologues:

Organism=Escherichia coli, GI1786212, Length=283, Percent_Identity=51.9434628975265, Blast_Score=301, Evalue=5e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003451 [H]

Pfam domain/function: PF02401 LYTB [H]

EC number: =1.17.1.2 [H]

Molecular weight: Translated: 40958; Mature: 40958

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKSRKSHDRSVLRFLRHLLENEYAIMTQHRPPLEIRLCGPRGFCAGVDRAIQIVVLALK
CCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHH
KYGAPVYVRHEIVHNRYVVEGLQARGAIFVEELDEIPAAHRNQPVVFSAHGVPKSVPADA
HCCCCEEEEHHHHHHHHHHHHHHHCCEEHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCC
EAKNLFYLDATCPLVSKVHKQAMRHQRLGRHVILIGHSGHPEVIGTMGQLPDGAVTLIET
CCCEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHCCCCCCCHHHHHHH
VEDAHTCHFDDEDNLGFVTQTTLSVDDTAGIIKELQARFPNLAAPAAESICYATTNRQDA
HHHCCEECCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCCHHCEEEEECCCHHH
VRAAAPGCDLFLIVGAPNSSNSKRLVEVAEKAGARMSMLVQRAEDIEWEQIGDISVVGLS
HHHCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCHHHCCCEEEEEEC
AGASAPEIIVDEIIDAFKARFDVKIELAETTVETENFLVNREIRDVELTVKDMAFVNGEH
CCCCCCHHHHHHHHHHHHHCEEEEEEEECCEECCCCEEECCCCCEEEEEEEHHHEECCCC
RVVGISKLMQGK
EEEEHHHHHCCC
>Mature Secondary Structure
MEKSRKSHDRSVLRFLRHLLENEYAIMTQHRPPLEIRLCGPRGFCAGVDRAIQIVVLALK
CCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHH
KYGAPVYVRHEIVHNRYVVEGLQARGAIFVEELDEIPAAHRNQPVVFSAHGVPKSVPADA
HCCCCEEEEHHHHHHHHHHHHHHHCCEEHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCC
EAKNLFYLDATCPLVSKVHKQAMRHQRLGRHVILIGHSGHPEVIGTMGQLPDGAVTLIET
CCCEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHCCCCCCCHHHHHHH
VEDAHTCHFDDEDNLGFVTQTTLSVDDTAGIIKELQARFPNLAAPAAESICYATTNRQDA
HHHCCEECCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCCHHCEEEEECCCHHH
VRAAAPGCDLFLIVGAPNSSNSKRLVEVAEKAGARMSMLVQRAEDIEWEQIGDISVVGLS
HHHCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCHHHCCCEEEEEEC
AGASAPEIIVDEIIDAFKARFDVKIELAETTVETENFLVNREIRDVELTVKDMAFVNGEH
CCCCCCHHHHHHHHHHHHHCEEEEEEEECCEECCCCEEECCCCCEEEEEEEHHHEECCCC
RVVGISKLMQGK
EEEEHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA