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The map label for this gene is glyS [H]

Identifier: 225851940

GI number: 225851940

Start: 428925

End: 431258

Strand: Direct

Name: glyS [H]

Synonym: BMEA_A0435

Alternate gene names: 225851940

Gene position: 428925-431258 (Clockwise)

Preceding gene: 225851939

Following gene: 225851941

Centisome position: 20.18

GC content: 59.98

Gene sequence:

>2334_bases
ATGCCTGATTTGTTGCTCGAACTCTTTTCCGAGGAAATTCCCGCTCGCATGCAGCGCAAGGCTGCCGGCGATCTGAAGAA
GATGATTACCGATGGCCTGGTGGATGCTGGCCTGACCTATGAGGCTGCGACCGCATACTGGACGCCGCGCCGTCTGGCGC
TCGATATTCGTGGCTTGACGGTACGTTCCAAGGACGTGCACGAGGACATCAAGGGGCCATCTGTTTCTGCGCCGGAACAG
GCCATTCAGGGGTTTTTGCGCAAGGCTGGCCTCAGCGATGTTTCTCAGGCGCATGTCCATTCCGATCCGAAGAAGGGTGA
TTTCTACGTCGCCCATCTGACGAAGCCGGGCCGTGCGGCGGAAGATATCGTCGCCGAACTGGTTCCGCAGACGATCCGCA
ACTTCCCATGGCCGAAATCCATGCGCTGGGGTGTGGCTTCGGCCCGGCCCGGTGCCTTGCGCTGGGTGCGTCCGCTGCAA
TCGATCCTGTGCACCTTCGGCCCTGAAACCGAGGAAACGAAGGTCATCGATTTCGATGTCGACGGTATCAAGTCCGGCAA
TGTCACCTATGGCCACCGTTTCCTGAGCGACGGTCAGGCGATCAAGGTGCGCCGCTTCGAGGATTATGTCGAAAAGCTCG
AAAAGGCTTTCGTAGTTCTCGATGCCGAGCGCCGCAAGGAAATCATTTCGCAGGATGCACATAATCTCGCCTTCGCTTCC
GGCCTTGAGCTGGTGGAAGACGACGGGCTTCTGGAAGAAGTGTCCGGTCTGGTCGAATGGCCGGTCGTGCTGATGGGCGA
ATTCGAGGAAGAATTTCTGGCCATCCCGCCGGAAGTCATCCGCCTTACCATCCGCGCCAACCAGAAATGCTTCGTCACCC
GCAAGCAGGGCGATGCTTCGAGGCTGGCTTCGCCAGCACCTCAGCATGAGGAGATACAGGCGCTTTCCAACAAGTTCATC
CTTGTCTCCAATGTTGCAGCGCGCGACGGCGGTACGGAAATCGCCTATGGCAACGGCAAGGTGGTGCGTGCGCGCCTGTC
GGATGCGCTCTATTTCTGGCATACCGACCAGCATGACCTGCCCGACCTCGATAAGCTGGCCGCATCGGCGGAAAAATTCG
GTCTTGATCTGAAGAAGCCGCTCGATCAGCGCATGGCGCGTCTGGATGCACTGAATGTGACCTTCCATGCCAAGCTTGGC
ACGCAAGGGCAGCGCGTGGAACGCATCCGCGAACTGGCAGCGCAGATTGCGCCGCTGGTGGGTGCCGACCCGAAGCTTGC
ACAGCGTGCGGCCGTGCTTGCCAAGGCTGACCTGCAAACCGAAGTTGTCGGCGAGTTTCCTGAACTTCAGGGCGCGATGG
GGCGCAAATATGCGTTGCTTCAGGGCGAAGACAAAGCCGTCGCCGCCGCTCTGGAAGAGCATTACAAGCCGCAGGGCCCG
TCCGACGTGGTGCCGAAGAACCCGGTTTCCGTTGCCGTGGCGCTTGCCGACAAGCTCGACACGCTGGTCGGATTCTGGGC
GATCGACGAAAAGCCGACCGGCTCGAAGGATCCTTATGCGCTGCGCCGTGCAGCATTGGGCGTCATTCGCCTGCTCCTGT
CGCAGGAATGGAAATTCCCGCTGCTGCCGCTTTTCCGCTCGGCCTTTGCCGCGTTGCAGGAAGGCCAGATGCAGCGCAAG
CTGCGTGAGTTTGAAGCAAAGCTTGCCGCGGAAAATTCCGGCGATGTGGATGGCGAGCAGGACGTGGATAATGCCCTCGC
CAATTACGAGAAGGAAATTCGCGCCGAAGCCGAAGCTTCTTGCGGGCCTGTGCTGGCCGATCTCCTCTCCTTCTTCCATG
ACCGTTTCAAGGTCCATCTGAAAGAAGAGGGCGCGCGCTACGACGCTATTGATGCGGTGTTGACGCCGGAAGCCGACAAT
CTGCTTCTGGTTGCGCGGCGCTTGGAGGCACTGATCGTCTTCATCAACGAGGTAGACGGCAAGAACCTTCTGGCCGGGGC
CAAGCGCGCGGCGAATATTCTCGCAGCCGAGGAAAAGAAGGGCACGAAGGTTGCCGACCGGGTTGACCCGGCCTTGCTGC
GTGAAGCGGAGGAAAAGGCGCTGTTCGATGCCGTTACCCTGGCCTCGCGCGAAGTCGGAGAAGCCATTGCCCGTGAGGAT
TTCAACGGCGCGATGCTGGCGCTTGCCAAGCTGCGTGGCCCGGTTGATATCTTCTTCGACAAGGTGCTGGTCAATGACGA
GGACGGGAATGTTCGCGCCAACCGGCTGGCGCTGCTCGACCAGATCCGCGCGGCCACAGGCAAGGTGGCCGATTTCTCGA
AGATCGCCGGATAA

Upstream 100 bases:

>100_bases
ACATTCTGCGCGTGCGCAACCTTGCGCGCCAGTGCGGCGAGGCATTCCTTTTGACGGATGCCGGTGGTTTCAATTTCAAG
CGTGAGGGCGAGTGAAGAAC

Downstream 100 bases:

>100_bases
GAGCCTGAGGGGCGCCGCGAGGCGCCCTTTTTATTGGCTGGATCATTCTCATCTTGCATGAAGTTGGTGCCGGATGCGCC
CGGATGTTGCTTGCCCGGCG

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 777; Mature: 776

Protein sequence:

>777_residues
MPDLLLELFSEEIPARMQRKAAGDLKKMITDGLVDAGLTYEAATAYWTPRRLALDIRGLTVRSKDVHEDIKGPSVSAPEQ
AIQGFLRKAGLSDVSQAHVHSDPKKGDFYVAHLTKPGRAAEDIVAELVPQTIRNFPWPKSMRWGVASARPGALRWVRPLQ
SILCTFGPETEETKVIDFDVDGIKSGNVTYGHRFLSDGQAIKVRRFEDYVEKLEKAFVVLDAERRKEIISQDAHNLAFAS
GLELVEDDGLLEEVSGLVEWPVVLMGEFEEEFLAIPPEVIRLTIRANQKCFVTRKQGDASRLASPAPQHEEIQALSNKFI
LVSNVAARDGGTEIAYGNGKVVRARLSDALYFWHTDQHDLPDLDKLAASAEKFGLDLKKPLDQRMARLDALNVTFHAKLG
TQGQRVERIRELAAQIAPLVGADPKLAQRAAVLAKADLQTEVVGEFPELQGAMGRKYALLQGEDKAVAAALEEHYKPQGP
SDVVPKNPVSVAVALADKLDTLVGFWAIDEKPTGSKDPYALRRAALGVIRLLLSQEWKFPLLPLFRSAFAALQEGQMQRK
LREFEAKLAAENSGDVDGEQDVDNALANYEKEIRAEAEASCGPVLADLLSFFHDRFKVHLKEEGARYDAIDAVLTPEADN
LLLVARRLEALIVFINEVDGKNLLAGAKRAANILAAEEKKGTKVADRVDPALLREAEEKALFDAVTLASREVGEAIARED
FNGAMLALAKLRGPVDIFFDKVLVNDEDGNVRANRLALLDQIRAATGKVADFSKIAG

Sequences:

>Translated_777_residues
MPDLLLELFSEEIPARMQRKAAGDLKKMITDGLVDAGLTYEAATAYWTPRRLALDIRGLTVRSKDVHEDIKGPSVSAPEQ
AIQGFLRKAGLSDVSQAHVHSDPKKGDFYVAHLTKPGRAAEDIVAELVPQTIRNFPWPKSMRWGVASARPGALRWVRPLQ
SILCTFGPETEETKVIDFDVDGIKSGNVTYGHRFLSDGQAIKVRRFEDYVEKLEKAFVVLDAERRKEIISQDAHNLAFAS
GLELVEDDGLLEEVSGLVEWPVVLMGEFEEEFLAIPPEVIRLTIRANQKCFVTRKQGDASRLASPAPQHEEIQALSNKFI
LVSNVAARDGGTEIAYGNGKVVRARLSDALYFWHTDQHDLPDLDKLAASAEKFGLDLKKPLDQRMARLDALNVTFHAKLG
TQGQRVERIRELAAQIAPLVGADPKLAQRAAVLAKADLQTEVVGEFPELQGAMGRKYALLQGEDKAVAAALEEHYKPQGP
SDVVPKNPVSVAVALADKLDTLVGFWAIDEKPTGSKDPYALRRAALGVIRLLLSQEWKFPLLPLFRSAFAALQEGQMQRK
LREFEAKLAAENSGDVDGEQDVDNALANYEKEIRAEAEASCGPVLADLLSFFHDRFKVHLKEEGARYDAIDAVLTPEADN
LLLVARRLEALIVFINEVDGKNLLAGAKRAANILAAEEKKGTKVADRVDPALLREAEEKALFDAVTLASREVGEAIARED
FNGAMLALAKLRGPVDIFFDKVLVNDEDGNVRANRLALLDQIRAATGKVADFSKIAG
>Mature_776_residues
PDLLLELFSEEIPARMQRKAAGDLKKMITDGLVDAGLTYEAATAYWTPRRLALDIRGLTVRSKDVHEDIKGPSVSAPEQA
IQGFLRKAGLSDVSQAHVHSDPKKGDFYVAHLTKPGRAAEDIVAELVPQTIRNFPWPKSMRWGVASARPGALRWVRPLQS
ILCTFGPETEETKVIDFDVDGIKSGNVTYGHRFLSDGQAIKVRRFEDYVEKLEKAFVVLDAERRKEIISQDAHNLAFASG
LELVEDDGLLEEVSGLVEWPVVLMGEFEEEFLAIPPEVIRLTIRANQKCFVTRKQGDASRLASPAPQHEEIQALSNKFIL
VSNVAARDGGTEIAYGNGKVVRARLSDALYFWHTDQHDLPDLDKLAASAEKFGLDLKKPLDQRMARLDALNVTFHAKLGT
QGQRVERIRELAAQIAPLVGADPKLAQRAAVLAKADLQTEVVGEFPELQGAMGRKYALLQGEDKAVAAALEEHYKPQGPS
DVVPKNPVSVAVALADKLDTLVGFWAIDEKPTGSKDPYALRRAALGVIRLLLSQEWKFPLLPLFRSAFAALQEGQMQRKL
REFEAKLAAENSGDVDGEQDVDNALANYEKEIRAEAEASCGPVLADLLSFFHDRFKVHLKEEGARYDAIDAVLTPEADNL
LLVARRLEALIVFINEVDGKNLLAGAKRAANILAAEEKKGTKVADRVDPALLREAEEKALFDAVTLASREVGEAIAREDF
NGAMLALAKLRGPVDIFFDKVLVNDEDGNVRANRLALLDQIRAATGKVADFSKIAG

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=778, Percent_Identity=32.9048843187661, Blast_Score=345, Evalue=6e-96,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 85423; Mature: 85292

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDLLLELFSEEIPARMQRKAAGDLKKMITDGLVDAGLTYEAATAYWTPRRLALDIRGLT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHEECCCEEEEEEECEE
VRSKDVHEDIKGPSVSAPEQAIQGFLRKAGLSDVSQAHVHSDPKKGDFYVAHLTKPGRAA
EECHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCEEEEEECCCCCHH
EDIVAELVPQTIRNFPWPKSMRWGVASARPGALRWVRPLQSILCTFGPETEETKVIDFDV
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEC
DGIKSGNVTYGHRFLSDGQAIKVRRFEDYVEKLEKAFVVLDAERRKEIISQDAHNLAFAS
CCCCCCCEEECHHHHCCCCEEEHHHHHHHHHHHHHHEEEEEHHHHHHHHHCCHHHHHHHH
GLELVEDDGLLEEVSGLVEWPVVLMGEFEEEFLAIPPEVIRLTIRANQKCFVTRKQGDAS
CCHHHCCCCHHHHHHHHHHCHHHHHCCCCHHHHCCCHHHEEEEEECCCEEEEEECCCCHH
RLASPAPQHEEIQALSNKFILVSNVAARDGGTEIAYGNGKVVRARLSDALYFWHTDQHDL
HHCCCCCCHHHHHHHHCCEEEEEECCCCCCCCEEEECCCCEEEEECCCCEEEEECCCCCC
PDLDKLAASAEKFGLDLKKPLDQRMARLDALNVTFHAKLGTQGQRVERIRELAAQIAPLV
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCEEEEEECCCCCHHHHHHHHHHHHHHHHC
GADPKLAQRAAVLAKADLQTEVVGEFPELQGAMGRKYALLQGEDKAVAAALEEHYKPQGP
CCCHHHHHHHHHHHHHCCHHHHHHCCHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCC
SDVVPKNPVSVAVALADKLDTLVGFWAIDEKPTGSKDPYALRRAALGVIRLLLSQEWKFP
CCCCCCCCHHHHHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
LLPLFRSAFAALQEGQMQRKLREFEAKLAAENSGDVDGEQDVDNALANYEKEIRAEAEAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
CGPVLADLLSFFHDRFKVHLKEEGARYDAIDAVLTPEADNLLLVARRLEALIVFINEVDG
CCHHHHHHHHHHHHHHEEEEHHCCCCCHHHHEEECCCCCCHHHHHHHHHHHHHEEECCCC
KNLLAGAKRAANILAAEEKKGTKVADRVDPALLREAEEKALFDAVTLASREVGEAIARED
CHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
FNGAMLALAKLRGPVDIFFDKVLVNDEDGNVRANRLALLDQIRAATGKVADFSKIAG
CCHHHHHHHHHCCCHHHHHHHHEEECCCCCEEHHHHHHHHHHHHHHCCCHHHHHHCC
>Mature Secondary Structure 
PDLLLELFSEEIPARMQRKAAGDLKKMITDGLVDAGLTYEAATAYWTPRRLALDIRGLT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHEECCCEEEEEEECEE
VRSKDVHEDIKGPSVSAPEQAIQGFLRKAGLSDVSQAHVHSDPKKGDFYVAHLTKPGRAA
EECHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCEEEEEECCCCCHH
EDIVAELVPQTIRNFPWPKSMRWGVASARPGALRWVRPLQSILCTFGPETEETKVIDFDV
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEC
DGIKSGNVTYGHRFLSDGQAIKVRRFEDYVEKLEKAFVVLDAERRKEIISQDAHNLAFAS
CCCCCCCEEECHHHHCCCCEEEHHHHHHHHHHHHHHEEEEEHHHHHHHHHCCHHHHHHHH
GLELVEDDGLLEEVSGLVEWPVVLMGEFEEEFLAIPPEVIRLTIRANQKCFVTRKQGDAS
CCHHHCCCCHHHHHHHHHHCHHHHHCCCCHHHHCCCHHHEEEEEECCCEEEEEECCCCHH
RLASPAPQHEEIQALSNKFILVSNVAARDGGTEIAYGNGKVVRARLSDALYFWHTDQHDL
HHCCCCCCHHHHHHHHCCEEEEEECCCCCCCCEEEECCCCEEEEECCCCEEEEECCCCCC
PDLDKLAASAEKFGLDLKKPLDQRMARLDALNVTFHAKLGTQGQRVERIRELAAQIAPLV
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCEEEEEECCCCCHHHHHHHHHHHHHHHHC
GADPKLAQRAAVLAKADLQTEVVGEFPELQGAMGRKYALLQGEDKAVAAALEEHYKPQGP
CCCHHHHHHHHHHHHHCCHHHHHHCCHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCC
SDVVPKNPVSVAVALADKLDTLVGFWAIDEKPTGSKDPYALRRAALGVIRLLLSQEWKFP
CCCCCCCCHHHHHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
LLPLFRSAFAALQEGQMQRKLREFEAKLAAENSGDVDGEQDVDNALANYEKEIRAEAEAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
CGPVLADLLSFFHDRFKVHLKEEGARYDAIDAVLTPEADNLLLVARRLEALIVFINEVDG
CCHHHHHHHHHHHHHHEEEEHHCCCCCHHHHEEECCCCCCHHHHHHHHHHHHHEEECCCC
KNLLAGAKRAANILAAEEKKGTKVADRVDPALLREAEEKALFDAVTLASREVGEAIARED
CHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
FNGAMLALAKLRGPVDIFFDKVLVNDEDGNVRANRLALLDQIRAATGKVADFSKIAG
CCHHHHHHHHHCCCHHHHHHHHEEECCCCCEEHHHHHHHHHHHHHHCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]