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The map label for this gene is ada [H]

Identifier: 225851911

GI number: 225851911

Start: 400289

End: 401374

Strand: Direct

Name: ada [H]

Synonym: BMEA_A0405

Alternate gene names: 225851911

Gene position: 400289-401374 (Clockwise)

Preceding gene: 225851910

Following gene: 225851912

Centisome position: 18.83

GC content: 59.58

Gene sequence:

>1086_bases
ATGAACCTTATGATTCCGAAAACGCATGACCCCGACGAAAGCCGCTGGCAGAAGGTGCTCGACCGTGACAAGGCATCGGA
CGGGAAGTTCGTCTATGCCGTGCGCACCACAGGTGTTTATTGCCGCCCATCCTGCCCATCGCGGCGGGGAAAGCGCGAAA
ACGTGCAGTTTTTCAACGGCTGCGAGGATGCCGAGCGCGCCGGTTTCCGCCCCTGTCTGCGCTGCAAGCCCGATCTTTCC
GATACGCTGGCGACGCAGAACAATGCCCGCCATGCCGAAATGGTCGCAAGCGCCTGCCGCTTCATTGAAACAGCGGAGAC
GCAGCCTTCGCTGGAGGAAATCGCGAATGTAGTTAAGGCAAGCCCGGCGCATTTCCATCGTGTTTTCAAGGCCTTCACCG
GCCTTACGCCGAAAGCCTATGCCGATGCGCACCGGGCAGGCAGGATGCGCGCAGCACTTGATATGCCGCAAATCCGGGTG
ACGGATGCGATCTATGATGCGGGATATAATTCCTCAAGCCGTTTCTATGAAGCGTCTGACCGGATTTTGGGAATGACCCC
AAAGGCTTATCGGGCTGGCGGCAAGGATGCCGATATCCGTTTCGCCATCGGACAATCAACGCTGGGCGCGGTGCTGGTGG
CGGCAAGCGGAAAGGGCGTCTGCGCCATTTTCATGGGCGACGATCCGCAAGGCTTGATCCATGATCTGGAAAAGCGTTTT
CCGAAGGCAAATCTGATCGGTGCTGACCGCAATTTCGAGAATCTGGTAGCCGAGGTGGTGGGCTTCGTGGAAGCGCCGGG
TATCGGTCTTGATCTGCCGCTGGATTTGCGCGGCACGGCGTTCCAGCAAAGGGTGTGGCGGGCGCTATGCGAAATTCCTG
CTGGGGAAACAGTAAGTTATACTGACATCGCCGAACGGATCAGTGCGCCACGGGCCGTTCGCGCTGTGGCGCAGGCCTGC
GCGGCAAACAAGATCGCCGTTGCCGTTCCCTGCCACCGCGTGGTGCGCAACGATGGCGGCATTTCCGGCTATCGCTGGGG
CGTGGAACGCAAGCGCGACCTGCTTGAAAGGGAGCGCAAGGCATGA

Upstream 100 bases:

>100_bases
TCTGTTCAGAACGGCTGGGAATTTGAAAGCAGGCAGCGGAGTGTTGGTTTCGCGCCATGGCATTAGGCTTCCTTCATGCT
CATATTGAAGGAATACGGAC

Downstream 100 bases:

>100_bases
TCGGGCATAAGCCTTGACGGTGTTTTTGAAACAATGTCGCAAGGCGCCCGGTATCATCTTTCCCGATTCCCGATCGTGCA
ATTTGCCAGCCTGTATAAAG

Product: methylated-DNA-protein-cysteine methyltransferase

Products: NA

Alternate protein names: Regulatory protein of adaptative response; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MNLMIPKTHDPDESRWQKVLDRDKASDGKFVYAVRTTGVYCRPSCPSRRGKRENVQFFNGCEDAERAGFRPCLRCKPDLS
DTLATQNNARHAEMVASACRFIETAETQPSLEEIANVVKASPAHFHRVFKAFTGLTPKAYADAHRAGRMRAALDMPQIRV
TDAIYDAGYNSSSRFYEASDRILGMTPKAYRAGGKDADIRFAIGQSTLGAVLVAASGKGVCAIFMGDDPQGLIHDLEKRF
PKANLIGADRNFENLVAEVVGFVEAPGIGLDLPLDLRGTAFQQRVWRALCEIPAGETVSYTDIAERISAPRAVRAVAQAC
AANKIAVAVPCHRVVRNDGGISGYRWGVERKRDLLERERKA

Sequences:

>Translated_361_residues
MNLMIPKTHDPDESRWQKVLDRDKASDGKFVYAVRTTGVYCRPSCPSRRGKRENVQFFNGCEDAERAGFRPCLRCKPDLS
DTLATQNNARHAEMVASACRFIETAETQPSLEEIANVVKASPAHFHRVFKAFTGLTPKAYADAHRAGRMRAALDMPQIRV
TDAIYDAGYNSSSRFYEASDRILGMTPKAYRAGGKDADIRFAIGQSTLGAVLVAASGKGVCAIFMGDDPQGLIHDLEKRF
PKANLIGADRNFENLVAEVVGFVEAPGIGLDLPLDLRGTAFQQRVWRALCEIPAGETVSYTDIAERISAPRAVRAVAQAC
AANKIAVAVPCHRVVRNDGGISGYRWGVERKRDLLERERKA
>Mature_361_residues
MNLMIPKTHDPDESRWQKVLDRDKASDGKFVYAVRTTGVYCRPSCPSRRGKRENVQFFNGCEDAERAGFRPCLRCKPDLS
DTLATQNNARHAEMVASACRFIETAETQPSLEEIANVVKASPAHFHRVFKAFTGLTPKAYADAHRAGRMRAALDMPQIRV
TDAIYDAGYNSSSRFYEASDRILGMTPKAYRAGGKDADIRFAIGQSTLGAVLVAASGKGVCAIFMGDDPQGLIHDLEKRF
PKANLIGADRNFENLVAEVVGFVEAPGIGLDLPLDLRGTAFQQRVWRALCEIPAGETVSYTDIAERISAPRAVRAVAQAC
AANKIAVAVPCHRVVRNDGGISGYRWGVERKRDLLERERKA

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB [H]

COG id: COG2169

COG function: function code F; Adenosine deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788542, Length=348, Percent_Identity=50.8620689655172, Blast_Score=350, Evalue=9e-98,
Organism=Escherichia coli, GI1787596, Length=87, Percent_Identity=44.8275862068966, Blast_Score=82, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI115533070, Length=80, Percent_Identity=57.5, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI115533068, Length=80, Percent_Identity=57.5, Blast_Score=99, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6320001, Length=85, Percent_Identity=43.5294117647059, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI17137554, Length=161, Percent_Identity=37.2670807453416, Blast_Score=82, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR016221
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF01035 DNA_binding_1; PF00165 HTH_AraC; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 39719; Mature: 39719

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: PS01124 HTH_ARAC_FAMILY_2 ; PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLMIPKTHDPDESRWQKVLDRDKASDGKFVYAVRTTGVYCRPSCPSRRGKRENVQFFNG
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCEEECCCCCCCCCCCCCCHHHCC
CEDAERAGFRPCLRCKPDLSDTLATQNNARHAEMVASACRFIETAETQPSLEEIANVVKA
CCHHHHCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHC
SPAHFHRVFKAFTGLTPKAYADAHRAGRMRAALDMPQIRVTDAIYDAGYNSSSRFYEASD
CHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHCCCCCEEEHHHHHHCCCCCCCCEEECCC
RILGMTPKAYRAGGKDADIRFAIGQSTLGAVLVAASGKGVCAIFMGDDPQGLIHDLEKRF
CEECCCCHHHHCCCCCCCEEEEECCCCCCEEEEEECCCCEEEEEECCCCHHHHHHHHHHC
PKANLIGADRNFENLVAEVVGFVEAPGIGLDLPLDLRGTAFQQRVWRALCEIPAGETVSY
CCCCEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEECH
TDIAERISAPRAVRAVAQACAANKIAVAVPCHRVVRNDGGISGYRWGVERKRDLLERERK
HHHHHHHCCCHHHHHHHHHHHCCCEEEEECHHHHHHCCCCCCHHHCCHHHHHHHHHHHHC
A
C
>Mature Secondary Structure
MNLMIPKTHDPDESRWQKVLDRDKASDGKFVYAVRTTGVYCRPSCPSRRGKRENVQFFNG
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCEEECCCCCCCCCCCCCCHHHCC
CEDAERAGFRPCLRCKPDLSDTLATQNNARHAEMVASACRFIETAETQPSLEEIANVVKA
CCHHHHCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHC
SPAHFHRVFKAFTGLTPKAYADAHRAGRMRAALDMPQIRVTDAIYDAGYNSSSRFYEASD
CHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHCCCCCEEEHHHHHHCCCCCCCCEEECCC
RILGMTPKAYRAGGKDADIRFAIGQSTLGAVLVAASGKGVCAIFMGDDPQGLIHDLEKRF
CEECCCCHHHHCCCCCCCEEEEECCCCCCEEEEEECCCCEEEEEECCCCHHHHHHHHHHC
PKANLIGADRNFENLVAEVVGFVEAPGIGLDLPLDLRGTAFQQRVWRALCEIPAGETVSY
CCCCEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEECH
TDIAERISAPRAVRAVAQACAANKIAVAVPCHRVVRNDGGISGYRWGVERKRDLLERERK
HHHHHHHCCCHHHHHHHHHHHCCCEEEEECHHHHHHCCCCCCHHHCCHHHHHHHHHHHHC
A
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987251; 3887409; 9097040; 9278503; 2982792; 3536913; 3009022; 3529081; 1581309; 8202360; 8500619; 8156986 [H]