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The map label for this gene is panB

Identifier: 225851875

GI number: 225851875

Start: 361513

End: 362340

Strand: Direct

Name: panB

Synonym: BMEA_A0368

Alternate gene names: 225851875

Gene position: 361513-362340 (Clockwise)

Preceding gene: 225851874

Following gene: 225851877

Centisome position: 17.01

GC content: 62.44

Gene sequence:

>828_bases
ATGAGTGCACCCGTAACCCGCAAACGGCTGACGCCGAAGGTTATTCAGGCGATGAAGGGCGAGTGTCCCATCGTCAGCCT
CACCGCTTATACGACGCCGGTTGCGCGTCTGCTCGACCCGCATTGCGACCTGCTTCTGGTGGGGGATTCGCTTGGCATGG
TACTTTACGGCATGGAGTCCACCCTTGCCGTCACGCTCGACATGATGATCATGCATGGGCAGGCGGTCATGCGCGGTACA
AGCCATGCCTGCGTGATCGTGGATATGCCTTTCGGCTCCTATCAGGAATCGAAGGAGCAGGCCTTCCGCAATGCCGCCCG
TGTGATGCAGGAAACGGGCTGTGACGGCGTGAAGCTGGAAGGCGGCGAGGAAATGGCCGAAACGGTGGCCTTTCTCGTCC
GGCGCGGCATTCCGGTCTTCGGCCATGTCGGGCTGATGCCGCAGCAGGTGAATACGGTCGGCGGGTTTCGTTCGCTGGGG
CGCGGCGACGATGAAGCCGGCAAGATACGCCGCGACGCGCAAGCCATTGCCCAAGCGGGTGCTTTCGCGGTGGTCATCGA
AGGCACGGTCGAGCCGCTTGCGCGCGAAATAACGGCCTTGATCGATATTCCGACCGTCGGTATCGGCGCTTCATCCGCCT
GCGACGGGCAGGTTCTGGTTTCGGATGACATGCTGGGCCTGTTCCAGGATTTCACGCCGCGTTTCGTCAAGCGTTTTGCG
CATCTGGCGCCGCAGGTGTCGCAAGCGGCCGAAGCCTATGCCGAAGAGGTGCGCGCGCGCAGATTTCCCGGCCCGGAACA
TGTTTTCGGGGCCAAGCCCGGAGCGTGA

Upstream 100 bases:

>100_bases
CTCGTTGCGCTTTTCGTGCGTGTGGGGGCGACGCGGCTTCTGGATAATCGTGTGATCGGACATGCGGCGCCACAGATCAC
ACAGGAAAGGGCAGCCTGAC

Downstream 100 bases:

>100_bases
GCACCGAAAATTGTGGCCGATGATATTGCCCATACCGATCTGATTGCCGTGCTGATTGCCGTGATTGAGGACGAACCCCG
CATCATGACCGTGCGGGGTT

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MSAPVTRKRLTPKVIQAMKGECPIVSLTAYTTPVARLLDPHCDLLLVGDSLGMVLYGMESTLAVTLDMMIMHGQAVMRGT
SHACVIVDMPFGSYQESKEQAFRNAARVMQETGCDGVKLEGGEEMAETVAFLVRRGIPVFGHVGLMPQQVNTVGGFRSLG
RGDDEAGKIRRDAQAIAQAGAFAVVIEGTVEPLAREITALIDIPTVGIGASSACDGQVLVSDDMLGLFQDFTPRFVKRFA
HLAPQVSQAAEAYAEEVRARRFPGPEHVFGAKPGA

Sequences:

>Translated_275_residues
MSAPVTRKRLTPKVIQAMKGECPIVSLTAYTTPVARLLDPHCDLLLVGDSLGMVLYGMESTLAVTLDMMIMHGQAVMRGT
SHACVIVDMPFGSYQESKEQAFRNAARVMQETGCDGVKLEGGEEMAETVAFLVRRGIPVFGHVGLMPQQVNTVGGFRSLG
RGDDEAGKIRRDAQAIAQAGAFAVVIEGTVEPLAREITALIDIPTVGIGASSACDGQVLVSDDMLGLFQDFTPRFVKRFA
HLAPQVSQAAEAYAEEVRARRFPGPEHVFGAKPGA
>Mature_274_residues
SAPVTRKRLTPKVIQAMKGECPIVSLTAYTTPVARLLDPHCDLLLVGDSLGMVLYGMESTLAVTLDMMIMHGQAVMRGTS
HACVIVDMPFGSYQESKEQAFRNAARVMQETGCDGVKLEGGEEMAETVAFLVRRGIPVFGHVGLMPQQVNTVGGFRSLGR
GDDEAGKIRRDAQAIAQAGAFAVVIEGTVEPLAREITALIDIPTVGIGASSACDGQVLVSDDMLGLFQDFTPRFVKRFAH
LAPQVSQAAEAYAEEVRARRFPGPEHVFGAKPGA

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=264, Percent_Identity=41.2878787878788, Blast_Score=187, Evalue=7e-49,
Organism=Saccharomyces cerevisiae, GI6319653, Length=259, Percent_Identity=33.976833976834, Blast_Score=155, Evalue=8e-39,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_BRUA1 (B2S9H9)

Other databases:

- EMBL:   CP000887
- RefSeq:   YP_001934344.1
- ProteinModelPortal:   B2S9H9
- SMR:   B2S9H9
- GeneID:   6328271
- GenomeReviews:   CP000887_GR
- KEGG:   bmc:BAbS19_I03290
- HOGENOM:   HBG299908
- OMA:   DMMIAHG
- ProtClustDB:   PRK00311
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 29418; Mature: 29287

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: NA

Important sites: ACT_SITE 187-187 BINDING 88-88 BINDING 118-118

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPVTRKRLTPKVIQAMKGECPIVSLTAYTTPVARLLDPHCDLLLVGDSLGMVLYGMES
CCCCCCHHHCCHHHHHHHCCCCCEEEEEHHHHHHHHHHCCCCCEEEECCCCCEEEECCCH
TLAVTLDMMIMHGQAVMRGTSHACVIVDMPFGSYQESKEQAFRNAARVMQETGCDGVKLE
HHHHHHHHHHHHCHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEC
GGEEMAETVAFLVRRGIPVFGHVGLMPQQVNTVGGFRSLGRGDDEAGKIRRDAQAIAQAG
CHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCC
AFAVVIEGTVEPLAREITALIDIPTVGIGASSACDGQVLVSDDMLGLFQDFTPRFVKRFA
CEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCHHHHHHHHCCHHHHHHHH
HLAPQVSQAAEAYAEEVRARRFPGPEHVFGAKPGA
HHCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SAPVTRKRLTPKVIQAMKGECPIVSLTAYTTPVARLLDPHCDLLLVGDSLGMVLYGMES
CCCCCHHHCCHHHHHHHCCCCCEEEEEHHHHHHHHHHCCCCCEEEECCCCCEEEECCCH
TLAVTLDMMIMHGQAVMRGTSHACVIVDMPFGSYQESKEQAFRNAARVMQETGCDGVKLE
HHHHHHHHHHHHCHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEC
GGEEMAETVAFLVRRGIPVFGHVGLMPQQVNTVGGFRSLGRGDDEAGKIRRDAQAIAQAG
CHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCC
AFAVVIEGTVEPLAREITALIDIPTVGIGASSACDGQVLVSDDMLGLFQDFTPRFVKRFA
CEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCHHHHHHHHCCHHHHHHHH
HLAPQVSQAAEAYAEEVRARRFPGPEHVFGAKPGA
HHCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA