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The map label for this gene is rph [H]

Identifier: 225851706

GI number: 225851706

Start: 185467

End: 186183

Strand: Direct

Name: rph [H]

Synonym: BMEA_A0179

Alternate gene names: 225851706

Gene position: 185467-186183 (Clockwise)

Preceding gene: 225851702

Following gene: 225851707

Centisome position: 8.72

GC content: 62.62

Gene sequence:

>717_bases
ATGCGTCCATCCAAGCGTGCTGCCGACGAAATGCGCACCATCTCTTTCGAGCGCGGCGTCTCCAAACATGCGGAAGGCTC
CTGCCTGGTCAAGTTTGGCGACACCCATGTTTTGTGCACGGCAAGCCTTGAGGAAAAAGTGCCGGGCTGGATGCGCAATA
CGGGCAAGGGCTGGGTGACGGCGGAATATGGTATGCTGCCGCGTTCCACCGGCGAGCGGATGCGCCGCGAGGCGGCGGCG
GGCAAGCAGGGCGGGCGCACGCAGGAAATCCAGCGCCTCATCGGCCGCTCGCTCCGCGCGGTCGTGGACATGCAGGCGCT
TGGCGAAATGCAGATCACGGTGGACTGCGATGTGATCCAGGCCGATGGCGGCACGCGCACGGCGGCTATCACCGGCGGCT
GGGTGGCGCTGCACGAATGCCTGCGCTGGATGGAAGCGCGCCAGATGGTGCGTGTCGAAAAGGTCCTGAAAGACCATGTA
GCCGCCATTTCCTGCGGCATTTATGAAGGCGTGCCGGTTCTCGATCTCGACTATGCCGAGGATTCGGTTGCCGAAACCGA
CAGCAATTTCGTCATGACCGGCAAGGGCGGCATCGTGGAAATTCAGGGCACCGCCGAAGGCGTGCCTTTCTCGGAAGAGG
AGTTTGGCGCGCTGATGAAGCTTGCGCGCAGCGGCATCGATCGTCTCGTATCGTTGCAGAAAATGGCCGTCGCCTGA

Upstream 100 bases:

>100_bases
GTGACATGCGAAATTTCTTTCTATTTATGCCTAACAGGCATAAAAAGCCGCACCTGAAAGTGAAATCGCTTTCCAAACCA
GTTTTGAAACGAGGGAAAAT

Downstream 100 bases:

>100_bases
CGGTTCCCCGTTATTCTGGCTCTGGCGGCCTGTAAATGAGGTTCTTTTTCGCTTCCGGTGCTCATGGAGTTTAAGTACAC
TGCGCTCCGGGTCTCAAAGG

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase [H]

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MRPSKRAADEMRTISFERGVSKHAEGSCLVKFGDTHVLCTASLEEKVPGWMRNTGKGWVTAEYGMLPRSTGERMRREAAA
GKQGGRTQEIQRLIGRSLRAVVDMQALGEMQITVDCDVIQADGGTRTAAITGGWVALHECLRWMEARQMVRVEKVLKDHV
AAISCGIYEGVPVLDLDYAEDSVAETDSNFVMTGKGGIVEIQGTAEGVPFSEEEFGALMKLARSGIDRLVSLQKMAVA

Sequences:

>Translated_238_residues
MRPSKRAADEMRTISFERGVSKHAEGSCLVKFGDTHVLCTASLEEKVPGWMRNTGKGWVTAEYGMLPRSTGERMRREAAA
GKQGGRTQEIQRLIGRSLRAVVDMQALGEMQITVDCDVIQADGGTRTAAITGGWVALHECLRWMEARQMVRVEKVLKDHV
AAISCGIYEGVPVLDLDYAEDSVAETDSNFVMTGKGGIVEIQGTAEGVPFSEEEFGALMKLARSGIDRLVSLQKMAVA
>Mature_238_residues
MRPSKRAADEMRTISFERGVSKHAEGSCLVKFGDTHVLCTASLEEKVPGWMRNTGKGWVTAEYGMLPRSTGERMRREAAA
GKQGGRTQEIQRLIGRSLRAVVDMQALGEMQITVDCDVIQADGGTRTAAITGGWVALHECLRWMEARQMVRVEKVLKDHV
AAISCGIYEGVPVLDLDYAEDSVAETDSNFVMTGKGGIVEIQGTAEGVPFSEEEFGALMKLARSGIDRLVSLQKMAVA

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates [H]

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family [H]

Homologues:

Organism=Escherichia coli, GI157672248, Length=211, Percent_Identity=59.7156398104265, Blast_Score=254, Evalue=5e-69,
Organism=Caenorhabditis elegans, GI71981632, Length=183, Percent_Identity=27.3224043715847, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336 [H]

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C [H]

EC number: =2.7.7.56 [H]

Molecular weight: Translated: 25937; Mature: 25937

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPSKRAADEMRTISFERGVSKHAEGSCLVKFGDTHVLCTASLEEKVPGWMRNTGKGWVT
CCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCEEEEECCHHHHCCHHHHCCCCCEEE
AEYGMLPRSTGERMRREAAAGKQGGRTQEIQRLIGRSLRAVVDMQALGEMQITVDCDVIQ
EECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEE
ADGGTRTAAITGGWVALHECLRWMEARQMVRVEKVLKDHVAAISCGIYEGVPVLDLDYAE
CCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
DSVAETDSNFVMTGKGGIVEIQGTAEGVPFSEEEFGALMKLARSGIDRLVSLQKMAVA
CHHHCCCCCEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRPSKRAADEMRTISFERGVSKHAEGSCLVKFGDTHVLCTASLEEKVPGWMRNTGKGWVT
CCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCEEEEECCHHHHCCHHHHCCCCCEEE
AEYGMLPRSTGERMRREAAAGKQGGRTQEIQRLIGRSLRAVVDMQALGEMQITVDCDVIQ
EECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEE
ADGGTRTAAITGGWVALHECLRWMEARQMVRVEKVLKDHVAAISCGIYEGVPVLDLDYAE
CCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
DSVAETDSNFVMTGKGGIVEIQGTAEGVPFSEEEFGALMKLARSGIDRLVSLQKMAVA
CHHHCCCCCEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA