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The map label for this gene is rpoN [H]

Identifier: 225851691

GI number: 225851691

Start: 174677

End: 176179

Strand: Direct

Name: rpoN [H]

Synonym: BMEA_A0164

Alternate gene names: 225851691

Gene position: 174677-176179 (Clockwise)

Preceding gene: 225851690

Following gene: 225851693

Centisome position: 8.22

GC content: 57.75

Gene sequence:

>1503_bases
ATGGCCCTGTCGCCTAAACTTGATATCCGTCAATCGCAATCGCTGGTGATGACACCCCAGCTCATGCAGTCGATCAAGCT
GCTACAGATGACGCATATCGAGCTCGATCAGTTTCTCGACATGGAAATTGAAAAAAATCCGCTTCTGGACAGGATCGAGG
CAGACAGTGATGATCTTGCGGGCGGCGAGCCTGCATCCGGCGCAGACGACCCCACTGAAAGCGCCGATAACTATGACTGG
TTCCAGACCGAGGGGGCGGGCGCCGCCGAAAACCTTTCCGCCACTTTCGATAATTCCCTGGAAAACATTTTTCCTGATGA
TCTGGGCTCCAGCCAAGAGCAATGGAAACTGTCTGCGGGCGAGGGGGCGTTTTCCGGCAACGGCTATGATATCGATCAGG
TAACGGCAGGCCGCCTGTCGCTCAGTGACTATGTGGCCGAACAGATCGTGCTTGGCTTCTCGAAGGCGGCCGACCGTTTC
ATCGCGACGGCGCTCGCCGATGCGCTGGATGAAAGCGGTTATCTTCGTATCGATATTGTCGGGCTTGCACACAATCTGGG
GATCGAGACCGCCGAGATCGGGCGCATTCTGGGTGTGCTGCAAGGCTTTGATCCACCAGGGCTTTTTGCGCGCGATCTGC
GTGAATGCCTTGCAATCCAGTTGCGTCTCAAGGACCGCTTCGATCCGGCGATGGAAGCCCTGATCGCCAATCTCGATCTT
CTGGCCCGGCGCGATTTTGCAACACTCCAGAAAATCTGCGGCGTCGATCAGGCGGACCTGATCGACATGCTGGCCGAAAT
CCGCGCGCTCGATCCGAAGCCCGGCGCGCGTTTTGAGGTGTCGGTTGCCGATACGATCGTGCCGGATGTGCTGGTTTTTT
CCGCGCGTGATGGTGGCTGGGCGATCGAGCTCAATCCGGCAACCATGCCCCGCCTCATCGTCAACAATGAATATTATGCC
GAGGTGAGCGGCTCGGTGAAGACAGAGGAAAAGGCATTTCTTACCGATTGCATGCAGACGGCAGGCTGGCTGGTGCGCAG
CCTTGATCAGCGTGCGCGCACCATCTTGAAAGTAGCGCAGGAGATCGTGCGCCAGCAGGATGGTTTCCTGCGCCATGGCG
TGGCGCATCTGAAGCCGCTGAACCTGCGCATGGTCGCCGATGCAGTGGGTATGCACGAATCGACCATCAGCCGCGTCACC
GCCAACAAGTTTATGGCAACCCCGCGCGGCGTGTTTGAATTGCGCTACTTCTTTACCGCTTCGCTTGCCTCATCGGAAGG
AGGCGAAGCCCATTCCTCCGAAAGCGTGCGCCATCGCATCCGCCAGATGATCGAGGCGGAAAAGCCCGATGAAGTTCTCT
CCGATGATGCCATCGTGGATGCGCTGAAAAAGGACGGTGTGGATATCGCCCGCCGCACCGTGGCGAAATATCGGGAAGCG
ATGAATATCGCTTCCTCCGCACAACGCCGGCGCGAAAAGAAGGCACGCTTTTCAACGCCGTAA

Upstream 100 bases:

>100_bases
AAATTGACAAGCAAGGTTCATACCAGTTTCCATTGGATTGCTGCGGCAAGAACAAGGGCACGAAACGCCTGAATGGAAAA
CCGGGGGAATAACGTTTCGC

Downstream 100 bases:

>100_bases
ACGCTAGCGCCAGAACCATTGTCCTCGCCGCTTTCATTTTGCCGGGTTTATTGTGTATCTTCGGGTTGTGCACAGGCCTG
CCCTGAGCAAAAATATATAT

Product: RNA polymerase factor sigma-54

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 500; Mature: 499

Protein sequence:

>500_residues
MALSPKLDIRQSQSLVMTPQLMQSIKLLQMTHIELDQFLDMEIEKNPLLDRIEADSDDLAGGEPASGADDPTESADNYDW
FQTEGAGAAENLSATFDNSLENIFPDDLGSSQEQWKLSAGEGAFSGNGYDIDQVTAGRLSLSDYVAEQIVLGFSKAADRF
IATALADALDESGYLRIDIVGLAHNLGIETAEIGRILGVLQGFDPPGLFARDLRECLAIQLRLKDRFDPAMEALIANLDL
LARRDFATLQKICGVDQADLIDMLAEIRALDPKPGARFEVSVADTIVPDVLVFSARDGGWAIELNPATMPRLIVNNEYYA
EVSGSVKTEEKAFLTDCMQTAGWLVRSLDQRARTILKVAQEIVRQQDGFLRHGVAHLKPLNLRMVADAVGMHESTISRVT
ANKFMATPRGVFELRYFFTASLASSEGGEAHSSESVRHRIRQMIEAEKPDEVLSDDAIVDALKKDGVDIARRTVAKYREA
MNIASSAQRRREKKARFSTP

Sequences:

>Translated_500_residues
MALSPKLDIRQSQSLVMTPQLMQSIKLLQMTHIELDQFLDMEIEKNPLLDRIEADSDDLAGGEPASGADDPTESADNYDW
FQTEGAGAAENLSATFDNSLENIFPDDLGSSQEQWKLSAGEGAFSGNGYDIDQVTAGRLSLSDYVAEQIVLGFSKAADRF
IATALADALDESGYLRIDIVGLAHNLGIETAEIGRILGVLQGFDPPGLFARDLRECLAIQLRLKDRFDPAMEALIANLDL
LARRDFATLQKICGVDQADLIDMLAEIRALDPKPGARFEVSVADTIVPDVLVFSARDGGWAIELNPATMPRLIVNNEYYA
EVSGSVKTEEKAFLTDCMQTAGWLVRSLDQRARTILKVAQEIVRQQDGFLRHGVAHLKPLNLRMVADAVGMHESTISRVT
ANKFMATPRGVFELRYFFTASLASSEGGEAHSSESVRHRIRQMIEAEKPDEVLSDDAIVDALKKDGVDIARRTVAKYREA
MNIASSAQRRREKKARFSTP
>Mature_499_residues
ALSPKLDIRQSQSLVMTPQLMQSIKLLQMTHIELDQFLDMEIEKNPLLDRIEADSDDLAGGEPASGADDPTESADNYDWF
QTEGAGAAENLSATFDNSLENIFPDDLGSSQEQWKLSAGEGAFSGNGYDIDQVTAGRLSLSDYVAEQIVLGFSKAADRFI
ATALADALDESGYLRIDIVGLAHNLGIETAEIGRILGVLQGFDPPGLFARDLRECLAIQLRLKDRFDPAMEALIANLDLL
ARRDFATLQKICGVDQADLIDMLAEIRALDPKPGARFEVSVADTIVPDVLVFSARDGGWAIELNPATMPRLIVNNEYYAE
VSGSVKTEEKAFLTDCMQTAGWLVRSLDQRARTILKVAQEIVRQQDGFLRHGVAHLKPLNLRMVADAVGMHESTISRVTA
NKFMATPRGVFELRYFFTASLASSEGGEAHSSESVRHRIRQMIEAEKPDEVLSDDAIVDALKKDGVDIARRTVAKYREAM
NIASSAQRRREKKARFSTP

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of the nitrogen fixation genes (nif operon), glnA and dctA for dicar

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=496, Percent_Identity=36.0887096774194, Blast_Score=306, Evalue=1e-84,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 55039; Mature: 54908

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALSPKLDIRQSQSLVMTPQLMQSIKLLQMTHIELDQFLDMEIEKNPLLDRIEADSDDLA
CCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCC
GGEPASGADDPTESADNYDWFQTEGAGAAENLSATFDNSLENIFPDDLGSSQEQWKLSAG
CCCCCCCCCCCCCCCCCCCCEECCCCCCHHCCHHHHHHHHHHCCCCCCCCCHHHEEEECC
EGAFSGNGYDIDQVTAGRLSLSDYVAEQIVLGFSKAADRFIATALADALDESGYLRIDIV
CCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
GLAHNLGIETAEIGRILGVLQGFDPPGLFARDLRECLAIQLRLKDRFDPAMEALIANLDL
EEHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
LARRDFATLQKICGVDQADLIDMLAEIRALDPKPGARFEVSVADTIVPDVLVFSARDGGW
HHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHCCCEEEECCCCCE
AIELNPATMPRLIVNNEYYAEVSGSVKTEEKAFLTDCMQTAGWLVRSLDQRARTILKVAQ
EEEECCCCCCEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIVRQQDGFLRHGVAHLKPLNLRMVADAVGMHESTISRVTANKFMATPRGVFELRYFFTA
HHHHHHCCHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
SLASSEGGEAHSSESVRHRIRQMIEAEKPDEVLSDDAIVDALKKDGVDIARRTVAKYREA
HHHCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
MNIASSAQRRREKKARFSTP
HHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
ALSPKLDIRQSQSLVMTPQLMQSIKLLQMTHIELDQFLDMEIEKNPLLDRIEADSDDLA
CCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCC
GGEPASGADDPTESADNYDWFQTEGAGAAENLSATFDNSLENIFPDDLGSSQEQWKLSAG
CCCCCCCCCCCCCCCCCCCCEECCCCCCHHCCHHHHHHHHHHCCCCCCCCCHHHEEEECC
EGAFSGNGYDIDQVTAGRLSLSDYVAEQIVLGFSKAADRFIATALADALDESGYLRIDIV
CCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
GLAHNLGIETAEIGRILGVLQGFDPPGLFARDLRECLAIQLRLKDRFDPAMEALIANLDL
EEHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
LARRDFATLQKICGVDQADLIDMLAEIRALDPKPGARFEVSVADTIVPDVLVFSARDGGW
HHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHCCCEEEECCCCCE
AIELNPATMPRLIVNNEYYAEVSGSVKTEEKAFLTDCMQTAGWLVRSLDQRARTILKVAQ
EEEECCCCCCEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIVRQQDGFLRHGVAHLKPLNLRMVADAVGMHESTISRVTANKFMATPRGVFELRYFFTA
HHHHHHCCHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
SLASSEGGEAHSSESVRHRIRQMIEAEKPDEVLSDDAIVDALKKDGVDIARRTVAKYREA
HHHCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
MNIASSAQRRREKKARFSTP
HHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2211497 [H]