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The map label for this gene is flhA [H]

Identifier: 225851667

GI number: 225851667

Start: 144707

End: 145819

Strand: Reverse

Name: flhA [H]

Synonym: BMEA_A0137

Alternate gene names: 225851667

Gene position: 145819-144707 (Counterclockwise)

Preceding gene: 225851668

Following gene: 225851666

Centisome position: 6.86

GC content: 61.01

Gene sequence:

>1113_bases
ATGAAGTCACGCGCAGCCGTTGCCTGGGAGGCAAAGAAGCCGCTCACCATCGAAACCGTCGAGATCGGCGGGCCAAAGGC
TGGAGAAGTGCTGGTCGAAATCATGGCGACGGGTGTTTGCCATACCGATGCCTACACACTTTCGGGCCTCGATTCGGAAG
GCAAGTTTCCGGCGATCCTCGGCCATGAAGGTGCGGGCATCGTGCGCGAAGTGGGCGCGGACGTAACATCGGTCAAGCCG
GGCGACCATGTCATTCCGCTCTACACACCTGAATGCCGCCAGTGCAAAACCTGCCTGTCGCAGCGCTCCAACCTGTGCAC
GGTCATCCGCGCCACGCAAGGCCAGGGCCTGATGCCCGACAAAACCTCGCGCTTTTCCTGCGATGGCGGCGAAGTGTTCC
ACTATATGGGCTGCTCGACCTTCTCGAACTTCACCGTCCTGCCGGAAATCGCGGTTGCCAAGGTGCGCGAGGACGCGCCT
TTCGACAAGATCTGCTATATCGGCTGCGGCGTCACGACGGGCATCGGCGCCGTGATCTATACGGCCAAGGTTCGCCCCGG
CTCGAATGTGGTGGTGTTCGGCCTCGGCGGCGTCGGCCTCAATGTCATTCAGGGCGCGCGCATGGTTGGCGCCGACAAGA
TCATCGGCGTGGACATCAATCCGGCCAAGGTCGAAATGGCACGGAAGTTCGGCATGACGGACTTCATCAACCCGCGCGAA
ATCGGCAATGACAAGGTGGTGCAGGCCATTCAGGATCTGACCGATGGCGGGGCGGACTATTCCTTCGACGCCACGGGCAA
TACCGACGTGATGCGTCAGGCGCTGGAATGCTGCCATCGCGGCTGGGGTGAATCGATCATTATCGGGGTGGCCGAAGCCG
GCAAGGAAATCGCCACCCGCCCGTTCCAGCTCGTCACCGGCCGCGTCTGGAAAGGCACCGCCTTCGGCGGCGCGCGCGGG
CGCACCGACGTGCCGAAGATCGTGGACTGGTACATGGAGGGCAAGATCAATATCGACGATCTCATCACTCACACCATGCC
GCTTGATGAAATCAACACCGCCTTCGACCTGATGCATGAAGGCAAATCGATCCGTTCGGTTGTCATCTACTGA

Upstream 100 bases:

>100_bases
GCAAAAAGACGATCCCGATACACAGATCGATGAAATCATGCGAATTTTGCGTACCTATCTGAAATAGGCGCGCAAAATAT
AACCGGAGGGACGCACCACC

Downstream 100 bases:

>100_bases
TGAACGCAAGCCTGCTCACCTCCCATTGGGCTGAAATCGACGATCTGGCCCCGCGCGCACTTTATGCGATGCTGAAACTG
CGCGTGGACGTCTTCGTGGT

Product: S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase

Products: NA

Alternate protein names: Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH [H]

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MKSRAAVAWEAKKPLTIETVEIGGPKAGEVLVEIMATGVCHTDAYTLSGLDSEGKFPAILGHEGAGIVREVGADVTSVKP
GDHVIPLYTPECRQCKTCLSQRSNLCTVIRATQGQGLMPDKTSRFSCDGGEVFHYMGCSTFSNFTVLPEIAVAKVREDAP
FDKICYIGCGVTTGIGAVIYTAKVRPGSNVVVFGLGGVGLNVIQGARMVGADKIIGVDINPAKVEMARKFGMTDFINPRE
IGNDKVVQAIQDLTDGGADYSFDATGNTDVMRQALECCHRGWGESIIIGVAEAGKEIATRPFQLVTGRVWKGTAFGGARG
RTDVPKIVDWYMEGKINIDDLITHTMPLDEINTAFDLMHEGKSIRSVVIY

Sequences:

>Translated_370_residues
MKSRAAVAWEAKKPLTIETVEIGGPKAGEVLVEIMATGVCHTDAYTLSGLDSEGKFPAILGHEGAGIVREVGADVTSVKP
GDHVIPLYTPECRQCKTCLSQRSNLCTVIRATQGQGLMPDKTSRFSCDGGEVFHYMGCSTFSNFTVLPEIAVAKVREDAP
FDKICYIGCGVTTGIGAVIYTAKVRPGSNVVVFGLGGVGLNVIQGARMVGADKIIGVDINPAKVEMARKFGMTDFINPRE
IGNDKVVQAIQDLTDGGADYSFDATGNTDVMRQALECCHRGWGESIIIGVAEAGKEIATRPFQLVTGRVWKGTAFGGARG
RTDVPKIVDWYMEGKINIDDLITHTMPLDEINTAFDLMHEGKSIRSVVIY
>Mature_370_residues
MKSRAAVAWEAKKPLTIETVEIGGPKAGEVLVEIMATGVCHTDAYTLSGLDSEGKFPAILGHEGAGIVREVGADVTSVKP
GDHVIPLYTPECRQCKTCLSQRSNLCTVIRATQGQGLMPDKTSRFSCDGGEVFHYMGCSTFSNFTVLPEIAVAKVREDAP
FDKICYIGCGVTTGIGAVIYTAKVRPGSNVVVFGLGGVGLNVIQGARMVGADKIIGVDINPAKVEMARKFGMTDFINPRE
IGNDKVVQAIQDLTDGGADYSFDATGNTDVMRQALECCHRGWGESIIIGVAEAGKEIATRPFQLVTGRVWKGTAFGGARG
RTDVPKIVDWYMEGKINIDDLITHTMPLDEINTAFDLMHEGKSIRSVVIY

Specific function: Essential for the oxidation of formaldehyde produced during methylotrophic growth [H]

COG id: COG1062

COG function: function code C; Zn-dependent alcohol dehydrogenases, class III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily [H]

Homologues:

Organism=Homo sapiens, GI71565154, Length=368, Percent_Identity=59.7826086956522, Blast_Score=449, Evalue=1e-126,
Organism=Homo sapiens, GI71565152, Length=374, Percent_Identity=48.3957219251337, Blast_Score=375, Evalue=1e-104,
Organism=Homo sapiens, GI34577061, Length=370, Percent_Identity=46.2162162162162, Blast_Score=365, Evalue=1e-101,
Organism=Homo sapiens, GI4501933, Length=370, Percent_Identity=45.6756756756757, Blast_Score=356, Evalue=2e-98,
Organism=Homo sapiens, GI4501929, Length=370, Percent_Identity=45.945945945946, Blast_Score=356, Evalue=2e-98,
Organism=Homo sapiens, GI262073058, Length=371, Percent_Identity=44.743935309973, Blast_Score=351, Evalue=7e-97,
Organism=Homo sapiens, GI71743840, Length=371, Percent_Identity=44.743935309973, Blast_Score=350, Evalue=8e-97,
Organism=Homo sapiens, GI156523966, Length=369, Percent_Identity=43.9024390243902, Blast_Score=341, Evalue=8e-94,
Organism=Homo sapiens, GI4501939, Length=362, Percent_Identity=44.1988950276243, Blast_Score=335, Evalue=6e-92,
Organism=Escherichia coli, GI1786552, Length=370, Percent_Identity=59.4594594594595, Blast_Score=446, Evalue=1e-126,
Organism=Escherichia coli, GI1788073, Length=363, Percent_Identity=26.9972451790634, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87082125, Length=383, Percent_Identity=27.154046997389, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI87081918, Length=339, Percent_Identity=29.7935103244838, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1790045, Length=353, Percent_Identity=25.4957507082153, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1787863, Length=362, Percent_Identity=24.585635359116, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI226510992, Length=380, Percent_Identity=23.9473684210526, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1786825, Length=387, Percent_Identity=24.5478036175711, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788075, Length=368, Percent_Identity=24.4565217391304, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1788407, Length=225, Percent_Identity=25.7777777777778, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI25146526, Length=373, Percent_Identity=55.7640750670241, Blast_Score=441, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI71997431, Length=375, Percent_Identity=55.4666666666667, Blast_Score=436, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI17565904, Length=343, Percent_Identity=54.2274052478134, Blast_Score=394, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17562584, Length=360, Percent_Identity=26.3888888888889, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17562878, Length=260, Percent_Identity=27.6923076923077, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17562582, Length=374, Percent_Identity=25.9358288770053, Blast_Score=72, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6320033, Length=371, Percent_Identity=55.7951482479784, Blast_Score=442, Evalue=1e-125,
Organism=Saccharomyces cerevisiae, GI6322619, Length=392, Percent_Identity=29.0816326530612, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6323729, Length=372, Percent_Identity=26.0752688172043, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6319955, Length=392, Percent_Identity=28.8265306122449, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6319621, Length=371, Percent_Identity=25.6064690026954, Blast_Score=90, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6323099, Length=392, Percent_Identity=25.765306122449, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323961, Length=363, Percent_Identity=23.1404958677686, Blast_Score=83, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6324486, Length=373, Percent_Identity=23.8605898123324, Blast_Score=83, Evalue=8e-17,
Organism=Drosophila melanogaster, GI17737895, Length=368, Percent_Identity=58.1521739130435, Blast_Score=463, Evalue=1e-131,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014183
- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.284 [H]

Molecular weight: Translated: 39683; Mature: 39683

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSRAAVAWEAKKPLTIETVEIGGPKAGEVLVEIMATGVCHTDAYTLSGLDSEGKFPAIL
CCCCCEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCEEE
GHEGAGIVREVGADVTSVKPGDHVIPLYTPECRQCKTCLSQRSNLCTVIRATQGQGLMPD
CCCCCCHHHHHCCCCEECCCCCCEEEEECCCHHHHHHHHHCCCCEEEEEEECCCCCCCCC
KTSRFSCDGGEVFHYMGCSTFSNFTVLPEIAVAKVREDAPFDKICYIGCGVTTGIGAVIY
CCCCEECCCCCEEEEECCCCCCCCEECHHHHHHHHHCCCCCCCEEEEECCHHHCCCEEEE
TAKVRPGSNVVVFGLGGVGLNVIQGARMVGADKIIGVDINPAKVEMARKFGMTDFINPRE
EEEECCCCCEEEEEECCCCHHHHHCCEEECCCEEEEECCCHHHHHHHHHCCCCCCCCHHH
IGNDKVVQAIQDLTDGGADYSFDATGNTDVMRQALECCHRGWGESIIIGVAEAGKEIATR
CCCHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHCCCCCEEEEEEHHCCHHHHCC
PFQLVTGRVWKGTAFGGARGRTDVPKIVDWYMEGKINIDDLITHTMPLDEINTAFDLMHE
CHHHHCCCEEECCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHC
GKSIRSVVIY
CCCCEEEEEC
>Mature Secondary Structure
MKSRAAVAWEAKKPLTIETVEIGGPKAGEVLVEIMATGVCHTDAYTLSGLDSEGKFPAIL
CCCCCEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCEEE
GHEGAGIVREVGADVTSVKPGDHVIPLYTPECRQCKTCLSQRSNLCTVIRATQGQGLMPD
CCCCCCHHHHHCCCCEECCCCCCEEEEECCCHHHHHHHHHCCCCEEEEEEECCCCCCCCC
KTSRFSCDGGEVFHYMGCSTFSNFTVLPEIAVAKVREDAPFDKICYIGCGVTTGIGAVIY
CCCCEECCCCCEEEEECCCCCCCCEECHHHHHHHHHCCCCCCCEEEEECCHHHCCCEEEE
TAKVRPGSNVVVFGLGGVGLNVIQGARMVGADKIIGVDINPAKVEMARKFGMTDFINPRE
EEEECCCCCEEEEEECCCCHHHHHCCEEECCCEEEEECCCHHHHHHHHHCCCCCCCCHHH
IGNDKVVQAIQDLTDGGADYSFDATGNTDVMRQALECCHRGWGESIIIGVAEAGKEIATR
CCCHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHCCCCCEEEEEEHHCCHHHHCC
PFQLVTGRVWKGTAFGGARGRTDVPKIVDWYMEGKINIDDLITHTMPLDEINTAFDLMHE
CHHHHCCCEEECCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHC
GKSIRSVVIY
CCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7798140 [H]