Click here to switch to the map view.

The map label for this gene is cysT [H]

Identifier: 225851648

GI number: 225851648

Start: 110888

End: 111754

Strand: Direct

Name: cysT [H]

Synonym: BMEA_A0115

Alternate gene names: 225851648

Gene position: 110888-111754 (Clockwise)

Preceding gene: 225851647

Following gene: 225851649

Centisome position: 5.22

GC content: 59.52

Gene sequence:

>867_bases
ATGTCTTCGCTCCCTGCACAGGCCAGAGCAGGGTGGCACTTCAGAAAGCCGAGCGTTATTCCCGGTTTCGGGCTGACGTT
CGGCTTCAGTGTTGCCTATCTGACCCTTATCATTCTCATTCCGCTGGCGGCTCTGGTGCTGCGTTCGAGCGATGTGGGCC
TTGCCGGCTTCTGGCGGATCGTCACCGACGAGCGCACGCTGAAGGCGCTTGAAACGAGCTTCGGCACCGCCTTCATCGCT
GCGCTGGTCAATGTGGTCTTTGGGGTCATTCTGGCTTGGGTTCTGGTGCGCTATCGCTTTGCGGGACGCCGTTTCGTCGA
TGCCATCGTGGATATTCCCTTTGCGCTGCCGACTGCCGTTGCAGGCATTTCGCTGGCCGCGATCTATGCGCCGAATGGCT
GGATCGGCGGCCTGCTCAGCCCTTATGGCATCCGCATCGCCTTTACGCGTATGGGCATCGTCATTGCGCTCATCTTCATC
GGACTGCCCTTCGTGGTGCGCACCGTGCAGCCGATCATCGAGGAAATCAGCCGCGAGGTGGAGGAGGCGGCGGCAACGCT
TGGCGCTTCCCGTTTCCAGACGATCTGGCGGGTGCTTCTGCCTGGCCTGCAACCGGCCATTATTACCGGCTTTGCGCTCG
CCTTTGCGCGCGGTATCGGCGAATATGGCTCCGTGATCTTCATCGCGGGCAATACGCCCTACAAGTCGGAGATTGCGCCA
CTTCTCATCGTCATCAAGCTTGAGGAATATGATTATGCGGGCGCGACGGCGCTGGCCACGGTGATGCTGGCGCTTTCTTT
CGCGATGCTTCTGTTCATCAATCTCATTCAGACACGCATACGGAGGAAATACGGCCATGGCGCATGA

Upstream 100 bases:

>100_bases
AAATAGCGGGAGCATGTCCCCGAAAGTGAGAACTTACGGCCTGGAAATTCTAAGCCAGGCCAACCGATAACGCAGCTGAC
AAGAGGGAACAAAACACCGC

Downstream 100 bases:

>100_bases
AGCCGCTTGCACCCGTCATGGCGCCGTGACCGAAAGCCCGCCGGTGCGTATTGCGCTGATTGCGCTGGCGCTTGCCTTTC
TGGCGCTCTTTCTCATCCTC

Product: sulfate ABC transporter permease CysT

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MSSLPAQARAGWHFRKPSVIPGFGLTFGFSVAYLTLIILIPLAALVLRSSDVGLAGFWRIVTDERTLKALETSFGTAFIA
ALVNVVFGVILAWVLVRYRFAGRRFVDAIVDIPFALPTAVAGISLAAIYAPNGWIGGLLSPYGIRIAFTRMGIVIALIFI
GLPFVVRTVQPIIEEISREVEEAAATLGASRFQTIWRVLLPGLQPAIITGFALAFARGIGEYGSVIFIAGNTPYKSEIAP
LLIVIKLEEYDYAGATALATVMLALSFAMLLFINLIQTRIRRKYGHGA

Sequences:

>Translated_288_residues
MSSLPAQARAGWHFRKPSVIPGFGLTFGFSVAYLTLIILIPLAALVLRSSDVGLAGFWRIVTDERTLKALETSFGTAFIA
ALVNVVFGVILAWVLVRYRFAGRRFVDAIVDIPFALPTAVAGISLAAIYAPNGWIGGLLSPYGIRIAFTRMGIVIALIFI
GLPFVVRTVQPIIEEISREVEEAAATLGASRFQTIWRVLLPGLQPAIITGFALAFARGIGEYGSVIFIAGNTPYKSEIAP
LLIVIKLEEYDYAGATALATVMLALSFAMLLFINLIQTRIRRKYGHGA
>Mature_287_residues
SSLPAQARAGWHFRKPSVIPGFGLTFGFSVAYLTLIILIPLAALVLRSSDVGLAGFWRIVTDERTLKALETSFGTAFIAA
LVNVVFGVILAWVLVRYRFAGRRFVDAIVDIPFALPTAVAGISLAAIYAPNGWIGGLLSPYGIRIAFTRMGIVIALIFIG
LPFVVRTVQPIIEEISREVEEAAATLGASRFQTIWRVLLPGLQPAIITGFALAFARGIGEYGSVIFIAGNTPYKSEIAPL
LIVIKLEEYDYAGATALATVMLALSFAMLLFINLIQTRIRRKYGHGA

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=269, Percent_Identity=47.2118959107807, Blast_Score=247, Evalue=5e-67,
Organism=Escherichia coli, GI87082099, Length=247, Percent_Identity=32.7935222672065, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1786980, Length=229, Percent_Identity=31.8777292576419, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787368, Length=228, Percent_Identity=29.8245614035088, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787369, Length=167, Percent_Identity=30.5389221556886, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1786254, Length=230, Percent_Identity=28.2608695652174, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31175; Mature: 31044

Theoretical pI: Translated: 10.32; Mature: 10.32

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLPAQARAGWHFRKPSVIPGFGLTFGFSVAYLTLIILIPLAALVLRSSDVGLAGFWRI
CCCCCCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
VTDERTLKALETSFGTAFIAALVNVVFGVILAWVLVRYRFAGRRFVDAIVDIPFALPTAV
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
AGISLAAIYAPNGWIGGLLSPYGIRIAFTRMGIVIALIFIGLPFVVRTVQPIIEEISREV
HHHHHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEAAATLGASRFQTIWRVLLPGLQPAIITGFALAFARGIGEYGSVIFIAGNTPYKSEIAP
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHCE
LLIVIKLEEYDYAGATALATVMLALSFAMLLFINLIQTRIRRKYGHGA
EEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SSLPAQARAGWHFRKPSVIPGFGLTFGFSVAYLTLIILIPLAALVLRSSDVGLAGFWRI
CCCCCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
VTDERTLKALETSFGTAFIAALVNVVFGVILAWVLVRYRFAGRRFVDAIVDIPFALPTAV
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
AGISLAAIYAPNGWIGGLLSPYGIRIAFTRMGIVIALIFIGLPFVVRTVQPIIEEISREV
HHHHHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEAAATLGASRFQTIWRVLLPGLQPAIITGFALAFARGIGEYGSVIFIAGNTPYKSEIAP
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHCE
LLIVIKLEEYDYAGATALATVMLALSFAMLLFINLIQTRIRRKYGHGA
EEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503; 2188959 [H]