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The map label for this gene is nfdA [H]

Identifier: 225686915

GI number: 225686915

Start: 1151244

End: 1152926

Strand: Direct

Name: nfdA [H]

Synonym: BMEA_B1167

Alternate gene names: 225686915

Gene position: 1151244-1152926 (Clockwise)

Preceding gene: 225686914

Following gene: 225686916

Centisome position: 97.11

GC content: 60.84

Gene sequence:

>1683_bases
ATGAACCATAAGGGAACGATATACATGACCAGCGCGACGCACGCAGATCTCATCCTCACCAATGGCCGCATTTATACTGT
CGATACTGCACGCCCCTGGGCGCAAGCCGTCGCCATTAGCGGCGGGCGCATTCTGGCGGTCGGCAGCACAGCCGAAATAG
AAGCCTATAGAACCGGTGAAACGGAAGTGGTGGACCTTGGCGGACGCATGGCCATGCCGGGTTTCGTGGATGTGCACAAT
CACATCATGATGGGCGGTCAGGCCGATCTTTTTGAAACGCAATTGCCCGCAGGCGCCAGCGTCGATGATATCTGCGCCCA
TGTGCGCCGTCAGGCCGAGGCAGCAGTGCCCGGCCAGTGGATTATCGCCAACCAGTGGGGCGCGGATATGATAACCGCGC
TCAACAGCGAGGCCTCGCTTGCCAAACTCGATGCGGCCAGCCTTGGCCATCCTGTCCTGCTGCGGGATGAAACCATGCAC
AATCGCTGGGTCAACAGCGTGGCATTGCAGATGTGCGGCATTGCCAACGACCAGCCGGACCCGCCCGCCGGTTCCTTCGG
GCGCGATCCCGCAACGGGGCGCCTGACCGGGCTTTTGGTTGAATCGGCTGCCGGTATTGTCGAGCGTGTTGCCGCCGATC
ACTTCACCGAGGCAATGGGCGAGGCAGCCATTGCCCGCGCGGTGGAAATCGTCAATTCCTATGGCGTCACCGCCTTTCAG
GATGCCGCCTCCGTCCTCCCGATCATGAAGGCGCTGACGGGGCTGGACAACAAGGGCAAGCTGACGGCCTGGGCCGTCAC
CTCCCTGCCCCTGATCGAGCCATCCTTCATGTTTGGCCTTTCCGGCGATGAGCTGCTTGCGCTGCGCGATGAATACCGCA
CCCGGCATGTCAGGCCGAATTTCACCAAGATATTCCTTGATGGCGTGCCCGGCGCGCGCACCAGCGCCTTCCATGATGCC
TATCTGGATGATGGCGTGCACCCGCGTGGCTATCGCGGCGAAACGCTCGTCACCTATCCCGATCTCGTCCAGCACATCGA
CAAGTCCGAAAAGCTTGGCATGGGACTGAAAATCCATTGCACCGGCGATTATGCAGTCACCGAAATGCTGGATGCGGTGG
AGGCCGTGCGCCATTTCAACGGCCCGGCCAAGGTGATGCACCACATCGCCCATGCAAGCTATGTCCGCCCGCAGGATGTC
GAACGCTTTGCAAAGCTTTCCGTCGTCGCCGATCTGTCTCCGATGATTTGGTATCCGACCACCTTCCTCGAAGGCCATAA
GGAAGCCATGGGCGATGAGCGCGCAACCCGCTTCTGGCCAATTGCCGATCTGCATAAATCGGGCGCGCTTCTGGCCGGAG
GCTCCGACTGGCCCGTCATTCCCGTGCCTGATCCATGGACGAGGATCGAAGGCATGGTCACGCGGCAAAACCCCTCCGGC
GCATTTCCGGGTGTTGCGCTCTGGCCGGAACAGGCCATCGATCTTGCAACCGCCATCCGCATCTTCACGCTCAATTCGGC
CATCGCCATGGGCATCGGGGCGGAAACAGGGTCCATTGAGCCTGGCAAGTCTGCGGACATCATCGTGCTCGACCAGCAGG
TCTTCGATGTGGCCCCGGACAGGATCGCGGATACGAAAGTCCTTACCACCTATTTTGCAGGCCGCGCCGTCTATCGGCGC
TGA

Upstream 100 bases:

>100_bases
TCATCATCAATCCTTGTACATCAAAATATTTAACGTGTGAAATATATTGACACGTTACCGCTGTCGCGGCAATCTAACAA
ACAAATAACGGGTTCAAATA

Downstream 100 bases:

>100_bases
CACCCTGGGCTGACACCAATAATTCACCCCGCCATGGCGGGAAAACAATAAACGGGGAAGAAGATCATGAAACCGATAAA
GGACACCTGCCTTCTGGTTG

Product: exoenzymes regulatory protein AepA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 560; Mature: 560

Protein sequence:

>560_residues
MNHKGTIYMTSATHADLILTNGRIYTVDTARPWAQAVAISGGRILAVGSTAEIEAYRTGETEVVDLGGRMAMPGFVDVHN
HIMMGGQADLFETQLPAGASVDDICAHVRRQAEAAVPGQWIIANQWGADMITALNSEASLAKLDAASLGHPVLLRDETMH
NRWVNSVALQMCGIANDQPDPPAGSFGRDPATGRLTGLLVESAAGIVERVAADHFTEAMGEAAIARAVEIVNSYGVTAFQ
DAASVLPIMKALTGLDNKGKLTAWAVTSLPLIEPSFMFGLSGDELLALRDEYRTRHVRPNFTKIFLDGVPGARTSAFHDA
YLDDGVHPRGYRGETLVTYPDLVQHIDKSEKLGMGLKIHCTGDYAVTEMLDAVEAVRHFNGPAKVMHHIAHASYVRPQDV
ERFAKLSVVADLSPMIWYPTTFLEGHKEAMGDERATRFWPIADLHKSGALLAGGSDWPVIPVPDPWTRIEGMVTRQNPSG
AFPGVALWPEQAIDLATAIRIFTLNSAIAMGIGAETGSIEPGKSADIIVLDQQVFDVAPDRIADTKVLTTYFAGRAVYRR

Sequences:

>Translated_560_residues
MNHKGTIYMTSATHADLILTNGRIYTVDTARPWAQAVAISGGRILAVGSTAEIEAYRTGETEVVDLGGRMAMPGFVDVHN
HIMMGGQADLFETQLPAGASVDDICAHVRRQAEAAVPGQWIIANQWGADMITALNSEASLAKLDAASLGHPVLLRDETMH
NRWVNSVALQMCGIANDQPDPPAGSFGRDPATGRLTGLLVESAAGIVERVAADHFTEAMGEAAIARAVEIVNSYGVTAFQ
DAASVLPIMKALTGLDNKGKLTAWAVTSLPLIEPSFMFGLSGDELLALRDEYRTRHVRPNFTKIFLDGVPGARTSAFHDA
YLDDGVHPRGYRGETLVTYPDLVQHIDKSEKLGMGLKIHCTGDYAVTEMLDAVEAVRHFNGPAKVMHHIAHASYVRPQDV
ERFAKLSVVADLSPMIWYPTTFLEGHKEAMGDERATRFWPIADLHKSGALLAGGSDWPVIPVPDPWTRIEGMVTRQNPSG
AFPGVALWPEQAIDLATAIRIFTLNSAIAMGIGAETGSIEPGKSADIIVLDQQVFDVAPDRIADTKVLTTYFAGRAVYRR
>Mature_560_residues
MNHKGTIYMTSATHADLILTNGRIYTVDTARPWAQAVAISGGRILAVGSTAEIEAYRTGETEVVDLGGRMAMPGFVDVHN
HIMMGGQADLFETQLPAGASVDDICAHVRRQAEAAVPGQWIIANQWGADMITALNSEASLAKLDAASLGHPVLLRDETMH
NRWVNSVALQMCGIANDQPDPPAGSFGRDPATGRLTGLLVESAAGIVERVAADHFTEAMGEAAIARAVEIVNSYGVTAFQ
DAASVLPIMKALTGLDNKGKLTAWAVTSLPLIEPSFMFGLSGDELLALRDEYRTRHVRPNFTKIFLDGVPGARTSAFHDA
YLDDGVHPRGYRGETLVTYPDLVQHIDKSEKLGMGLKIHCTGDYAVTEMLDAVEAVRHFNGPAKVMHHIAHASYVRPQDV
ERFAKLSVVADLSPMIWYPTTFLEGHKEAMGDERATRFWPIADLHKSGALLAGGSDWPVIPVPDPWTRIEGMVTRQNPSG
AFPGVALWPEQAIDLATAIRIFTLNSAIAMGIGAETGSIEPGKSADIIVLDQQVFDVAPDRIADTKVLTTYFAGRAVYRR

Specific function: Hydrolyzes N-substituted formamides, but not amides. N- benzylformamide is the preferred substrate, while N-butylformamide is hydrolyzed at a much lower rate. Has very low activity towards allylformamide, N-(2-cyclohex-1-enylethyl)formamide and N-(alpha-

COG id: COG1574

COG function: function code R; Predicted metal-dependent hydrolase with the TIM-barrel fold

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013108
- InterPro:   IPR011059 [H]

Pfam domain/function: PF07969 Amidohydro_3 [H]

EC number: =3.5.1.91 [H]

Molecular weight: Translated: 60479; Mature: 60479

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHKGTIYMTSATHADLILTNGRIYTVDTARPWAQAVAISGGRILAVGSTAEIEAYRTGE
CCCCCEEEEEECCCCEEEEECCEEEEEECCCCHHHHEEECCCEEEEECCCCEEEEEECCC
TEVVDLGGRMAMPGFVDVHNHIMMGGQADLFETQLPAGASVDDICAHVRRQAEAAVPGQW
CEEEECCCCCCCCCEEECCCEEEECCCCCHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCE
IIANQWGADMITALNSEASLAKLDAASLGHPVLLRDETMHNRWVNSVALQMCGIANDQPD
EEECCCCCHHHHHHCCCCHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCC
PPAGSFGRDPATGRLTGLLVESAAGIVERVAADHFTEAMGEAAIARAVEIVNSYGVTAFQ
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
DAASVLPIMKALTGLDNKGKLTAWAVTSLPLIEPSFMFGLSGDELLALRDEYRTRHVRPN
HHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCEEECCCCCCEEEEHHHHHHCCCCCC
FTKIFLDGVPGARTSAFHDAYLDDGVHPRGYRGETLVTYPDLVQHIDKSEKLGMGLKIHC
CEEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCEEEECHHHHHHCCCHHHCCCCEEEEE
TGDYAVTEMLDAVEAVRHFNGPAKVMHHIAHASYVRPQDVERFAKLSVVADLSPMIWYPT
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEECCH
TFLEGHKEAMGDERATRFWPIADLHKSGALLAGGSDWPVIPVPDPWTRIEGMVTRQNPSG
HHHCCHHHHCCCCCCCEECCHHHHCCCCEEEECCCCCCEEECCCCHHHHCCEEEECCCCC
AFPGVALWPEQAIDLATAIRIFTLNSAIAMGIGAETGSIEPGKSADIIVLDQQVFDVAPD
CCCCCEECCHHHHHHHHHEEEEEECCHHEEEECCCCCCCCCCCCCCEEEECHHHHHCCCC
RIADTKVLTTYFAGRAVYRR
HHCCHHHHHHHHHCHHHCCC
>Mature Secondary Structure
MNHKGTIYMTSATHADLILTNGRIYTVDTARPWAQAVAISGGRILAVGSTAEIEAYRTGE
CCCCCEEEEEECCCCEEEEECCEEEEEECCCCHHHHEEECCCEEEEECCCCEEEEEECCC
TEVVDLGGRMAMPGFVDVHNHIMMGGQADLFETQLPAGASVDDICAHVRRQAEAAVPGQW
CEEEECCCCCCCCCEEECCCEEEECCCCCHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCE
IIANQWGADMITALNSEASLAKLDAASLGHPVLLRDETMHNRWVNSVALQMCGIANDQPD
EEECCCCCHHHHHHCCCCHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCC
PPAGSFGRDPATGRLTGLLVESAAGIVERVAADHFTEAMGEAAIARAVEIVNSYGVTAFQ
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
DAASVLPIMKALTGLDNKGKLTAWAVTSLPLIEPSFMFGLSGDELLALRDEYRTRHVRPN
HHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCEEECCCCCCEEEEHHHHHHCCCCCC
FTKIFLDGVPGARTSAFHDAYLDDGVHPRGYRGETLVTYPDLVQHIDKSEKLGMGLKIHC
CEEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCEEEECHHHHHHCCCHHHCCCCEEEEE
TGDYAVTEMLDAVEAVRHFNGPAKVMHHIAHASYVRPQDVERFAKLSVVADLSPMIWYPT
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEECCH
TFLEGHKEAMGDERATRFWPIADLHKSGALLAGGSDWPVIPVPDPWTRIEGMVTRQNPSG
HHHCCHHHHCCCCCCCEECCHHHHCCCCEEEECCCCCCEEECCCCHHHHCCEEEECCCCC
AFPGVALWPEQAIDLATAIRIFTLNSAIAMGIGAETGSIEPGKSADIIVLDQQVFDVAPD
CCCCCEECCHHHHHHHHHEEEEEECCHHEEEECCCCCCCCCCCCCCEEEECHHHHHCCCC
RIADTKVLTTYFAGRAVYRR
HHCCHHHHHHHHHCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA