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The map label for this gene is puuP [H]

Identifier: 225686913

GI number: 225686913

Start: 1148042

End: 1149535

Strand: Direct

Name: puuP [H]

Synonym: BMEA_B1165

Alternate gene names: 225686913

Gene position: 1148042-1149535 (Clockwise)

Preceding gene: 225686906

Following gene: 225686914

Centisome position: 96.84

GC content: 60.71

Gene sequence:

>1494_bases
ATGTGGAGCGCGGTTTTTCAAGTATCCAGGGGAACAAATATCATGAGCAATCCGGGTGAACTGCCTGAACAAACCAATCA
ATTGCGAAAAAACAGCCTCGGTGTAGCGGCTGTAACCTTTCTCGTCGTCTCTGCGGCAGCGCCTTTGACTGCCGTAGCCG
GCGGCGTGCCGCTTTCCATGCTTCTTGGAAACGGGCCGGGCATTCCGCTTACTTTCCTGATCGTCACCGGCGTTCTTTTG
ATGTTTTCGGTGGGGTATGTCGCCATGGCGCGGCACATCCGCAATGCCGGCGCATTTTATGCCTTCACCGCGCAGGGGCT
GGGTGGCCTGATGGGTGGTGCGGCGGCGCTTCTCGCCATTCTGGCCTATAATGCCATGCAGATCGGCGTGTTCGGCCTTT
TCGGCGCGGCAACATCCGGCTTCATGGCCAGCCTCGGCCTCGACCTGCCATGGTGGGCATGGACCTATATCGGCATTGCA
GCCGTTGCCGTGCTCGGCTATCGCCGGGTCGATCTTTCGGCACGGGTTCTCACCGTGCTCGTGGTTCTGGAATATCTCGT
CGTGCTGGTGATTGATGCCGCCATCCTGTTTTCCGGCGGCGATAGCGGGCTTACGATGGAACCCTTCACGCCATCCGCCT
TCCTGAGCGGGACGCCCGCCATCGGCATTCTTTTCTGCTTTGCCGCCTTCATCGGCTTTGAAGCAACCACGGTCTATAGC
GAGGAAGCCCGCGATCCGGGCCGCACCGTGCCGCGCGCGACCTATATTTCCGTGCTGATTATCGGCATCTTCTACATGCT
GACCGCGTGGCTGATGGTCAATGGCGCGGGGGCGGGCAAGCTTGTCGGCGAATTGCAGGCTCTGCAAGACCCCACGACCT
TTCTCTTCGGCCTTGCCGAGCGTTATGTCGGCCACTGGATCATCCCGGTCATGAACCTGCTCTTCGTCACCAGCCTCTTT
GCAGGCGTGGTCGCCTTTCACAACGGCGTCGCCCGCTACATGTATGTGGCTGGCCGTGAAGGGCTGCTGCCGCGCGGGCT
TGGCGTCACCCATCCGCAATATCAAAGCCCGCATGTCGGCTCCATCGTCCAGACCGCAATTGCAATCACCGTCGTGGGGA
TATTCGCCGTCACCGGACAAGACCCTGTTCTGGCGCTCTTTTCCTGGGCAACCAATGTCGGCACGCTGGCAATCATGCTT
CTCATGACCTTCACGGCCTTTGCCATCATCGCCTTTTTTGCGCGCCGTCCGGGGCTGGAAAGCAACCCCTTCACCACGCG
CGTCCTGCCTCTGGTGACGGGCACCATCCTTGGCCTTCTGGTGATCTATATTGCCGTCAATTTCGGCAGCCTTGCCGGTG
CAAACGGCTTCATGGCCGTGTTCCTGCCGGGCCTTGTGCTGGTGGCGGCGATTGCGGGCCTGCTGCTTGCATTGCGGCTC
AAGGGCACCAATGCCGCGGGCTTCGCCCGGCTGGGCGCCGGACAGGCCGTCTGA

Upstream 100 bases:

>100_bases
AGTCTATATTTGAAGCATCGGTTTAAATAATGAACCGATCAAATCCGGGCTTAAACAATACAAAAACAGCCGGAATGGCG
CTTTCAACTTTGTATCCGCG

Downstream 100 bases:

>100_bases
CCGCTGAAATTCCGGCGCTCTCCCACAAGGAGGGCGCCCTATCTGCATTTGCAATTTGAGGATACACCGCCATGCAAGAC
AAGATCGACCGTCTGCGCCA

Product: amino acid permease-associated protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 497; Mature: 497

Protein sequence:

>497_residues
MWSAVFQVSRGTNIMSNPGELPEQTNQLRKNSLGVAAVTFLVVSAAAPLTAVAGGVPLSMLLGNGPGIPLTFLIVTGVLL
MFSVGYVAMARHIRNAGAFYAFTAQGLGGLMGGAAALLAILAYNAMQIGVFGLFGAATSGFMASLGLDLPWWAWTYIGIA
AVAVLGYRRVDLSARVLTVLVVLEYLVVLVIDAAILFSGGDSGLTMEPFTPSAFLSGTPAIGILFCFAAFIGFEATTVYS
EEARDPGRTVPRATYISVLIIGIFYMLTAWLMVNGAGAGKLVGELQALQDPTTFLFGLAERYVGHWIIPVMNLLFVTSLF
AGVVAFHNGVARYMYVAGREGLLPRGLGVTHPQYQSPHVGSIVQTAIAITVVGIFAVTGQDPVLALFSWATNVGTLAIML
LMTFTAFAIIAFFARRPGLESNPFTTRVLPLVTGTILGLLVIYIAVNFGSLAGANGFMAVFLPGLVLVAAIAGLLLALRL
KGTNAAGFARLGAGQAV

Sequences:

>Translated_497_residues
MWSAVFQVSRGTNIMSNPGELPEQTNQLRKNSLGVAAVTFLVVSAAAPLTAVAGGVPLSMLLGNGPGIPLTFLIVTGVLL
MFSVGYVAMARHIRNAGAFYAFTAQGLGGLMGGAAALLAILAYNAMQIGVFGLFGAATSGFMASLGLDLPWWAWTYIGIA
AVAVLGYRRVDLSARVLTVLVVLEYLVVLVIDAAILFSGGDSGLTMEPFTPSAFLSGTPAIGILFCFAAFIGFEATTVYS
EEARDPGRTVPRATYISVLIIGIFYMLTAWLMVNGAGAGKLVGELQALQDPTTFLFGLAERYVGHWIIPVMNLLFVTSLF
AGVVAFHNGVARYMYVAGREGLLPRGLGVTHPQYQSPHVGSIVQTAIAITVVGIFAVTGQDPVLALFSWATNVGTLAIML
LMTFTAFAIIAFFARRPGLESNPFTTRVLPLVTGTILGLLVIYIAVNFGSLAGANGFMAVFLPGLVLVAAIAGLLLALRL
KGTNAAGFARLGAGQAV
>Mature_497_residues
MWSAVFQVSRGTNIMSNPGELPEQTNQLRKNSLGVAAVTFLVVSAAAPLTAVAGGVPLSMLLGNGPGIPLTFLIVTGVLL
MFSVGYVAMARHIRNAGAFYAFTAQGLGGLMGGAAALLAILAYNAMQIGVFGLFGAATSGFMASLGLDLPWWAWTYIGIA
AVAVLGYRRVDLSARVLTVLVVLEYLVVLVIDAAILFSGGDSGLTMEPFTPSAFLSGTPAIGILFCFAAFIGFEATTVYS
EEARDPGRTVPRATYISVLIIGIFYMLTAWLMVNGAGAGKLVGELQALQDPTTFLFGLAERYVGHWIIPVMNLLFVTSLF
AGVVAFHNGVARYMYVAGREGLLPRGLGVTHPQYQSPHVGSIVQTAIAITVVGIFAVTGQDPVLALFSWATNVGTLAIML
LMTFTAFAIIAFFARRPGLESNPFTTRVLPLVTGTILGLLVIYIAVNFGSLAGANGFMAVFLPGLVLVAAIAGLLLALRL
KGTNAAGFARLGAGQAV

Specific function: Imports putrescine [H]

COG id: COG0531

COG function: function code E; Amino acid transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily [H]

Homologues:

Organism=Escherichia coli, GI87081869, Length=445, Percent_Identity=24.2696629213483, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87082023, Length=222, Percent_Identity=27.027027027027, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 51971; Mature: 51971

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWSAVFQVSRGTNIMSNPGELPEQTNQLRKNSLGVAAVTFLVVSAAAPLTAVAGGVPLSM
CCHHHEEECCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHCCCCHHH
LLGNGPGIPLTFLIVTGVLLMFSVGYVAMARHIRNAGAFYAFTAQGLGGLMGGAAALLAI
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHH
LAYNAMQIGVFGLFGAATSGFMASLGLDLPWWAWTYIGIAAVAVLGYRRVDLSARVLTVL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
VVLEYLVVLVIDAAILFSGGDSGLTMEPFTPSAFLSGTPAIGILFCFAAFIGFEATTVYS
HHHHHHHHHHHHHHHHEECCCCCCEECCCCCCHHHCCCCHHHHHHHHHHHHCCCHHEEEC
EEARDPGRTVPRATYISVLIIGIFYMLTAWLMVNGAGAGKLVGELQALQDPTTFLFGLAE
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHH
RYVGHWIIPVMNLLFVTSLFAGVVAFHNGVARYMYVAGREGLLPRGLGVTHPQYQSPHVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHEECCCCCCCCCCCCCCCCCCCCCHH
SIVQTAIAITVVGIFAVTGQDPVLALFSWATNVGTLAIMLLMTFTAFAIIAFFARRPGLE
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SNPFTTRVLPLVTGTILGLLVIYIAVNFGSLAGANGFMAVFLPGLVLVAAIAGLLLALRL
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
KGTNAAGFARLGAGQAV
CCCCCCCHHHCCCCCCC
>Mature Secondary Structure
MWSAVFQVSRGTNIMSNPGELPEQTNQLRKNSLGVAAVTFLVVSAAAPLTAVAGGVPLSM
CCHHHEEECCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHCCCCHHH
LLGNGPGIPLTFLIVTGVLLMFSVGYVAMARHIRNAGAFYAFTAQGLGGLMGGAAALLAI
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHH
LAYNAMQIGVFGLFGAATSGFMASLGLDLPWWAWTYIGIAAVAVLGYRRVDLSARVLTVL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
VVLEYLVVLVIDAAILFSGGDSGLTMEPFTPSAFLSGTPAIGILFCFAAFIGFEATTVYS
HHHHHHHHHHHHHHHHEECCCCCCEECCCCCCHHHCCCCHHHHHHHHHHHHCCCHHEEEC
EEARDPGRTVPRATYISVLIIGIFYMLTAWLMVNGAGAGKLVGELQALQDPTTFLFGLAE
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHH
RYVGHWIIPVMNLLFVTSLFAGVVAFHNGVARYMYVAGREGLLPRGLGVTHPQYQSPHVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHEECCCCCCCCCCCCCCCCCCCCCHH
SIVQTAIAITVVGIFAVTGQDPVLALFSWATNVGTLAIMLLMTFTAFAIIAFFARRPGLE
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SNPFTTRVLPLVTGTILGLLVIYIAVNFGSLAGANGFMAVFLPGLVLVAAIAGLLLALRL
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
KGTNAAGFARLGAGQAV
CCCCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]