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The map label for this gene is 225686836

Identifier: 225686836

GI number: 225686836

Start: 1046728

End: 1048146

Strand: Reverse

Name: 225686836

Synonym: BMEA_B1074

Alternate gene names: NA

Gene position: 1048146-1046728 (Counterclockwise)

Preceding gene: 225686837

Following gene: 225686831

Centisome position: 88.41

GC content: 58.7

Gene sequence:

>1419_bases
TTGGACCTGACCGAACTTTCCGCCGCGCGCCTTGTGAACATGATGGGAAAAAGGCAGACTTCCCCCTCCGAAGTGATGGC
CGCGCATCTTGATCGTATCGAGCGGCATAACGGAGCCGTCAACGCCATGGTCGCCATGCGTTCCCGCGACGAGCTGATTG
CCGAAGCCCGGTTGCTGGATGACAAACCAGTTTGCGGGCCACTGCATGGATTGCCCTGGGCGATCAAGGATCTTCTGCCA
ACAAAAGATATTCGCTCCACCTGGGGCTCTCCTATTCATGCTGATTACATTCCGGCAGAAGATGCCCTAGCAGTGAGCCG
CATCCGAAAGGCCGGCGCCGTCATCATGGGCAAGACAAATACGCCCGAATGGGGCCATGGTAGCCACAGTTTCAATCCCG
TCTACGGCGTCACCTGTAATCCCTACGATACTCAATTAAGTGCCGGTGGCTCCTCGGGCGGAACTGCAGCCGCGCTTGCC
GCCCACTTTATGCCCCTGGGCGACGGATCGGATATGATGGGAAGTCTGCGCAATCCCGCAGGCTTCTGCAATATTTATGG
CTATCGGCCAAGCTGGGGACTTGTGCCCAATGAAATTGGCGGCGATCTCTTTGTACACACCATGGCCACTCTGGGCCCCA
TGGCACGTGATATTGAGGATCTCGTTCTGCTGCTTGACGTTCTGGCCGAGCCGTATTCACTGAGCCCTTTTGGACAGAAG
CACACCGGGTTTGCCACGAACCACGCACCCGCAGACCTTCGGGGCAAACGCATTGCCTGGCTTGGCAACTGGGGAGGAGC
ATATCCGTGTGAAGCAGGCATTCTGGAGCGTTGCGAGAAGGGCCTTGCCGTCCTGAGCGAGCTTGGCGCCGAGATCGTCC
CGCTCGAACCGCCTTTTTCTTCCCAAGCCCTATGGGAAGCATGGATCACCCTTCGTTCACTGGTGATCTATGGTTCCAAG
CGCGCCCTATGGGAAAAGCCGGAAACCCGGAAGCTCATCAAGCCCGAAACGCTTTGGGAAATTGAAAACGGCGCGGGGCT
GACCGCACAACAGATCATGCATGCGAGCCAGATACGCTCGGACTGGTATGTCAGCGCCCACAGGCTGTTTGCCGAATATG
ACGCCCTGATTATGCCGACGGCCCAGGTCTGGCCTTTTCCAGCTGACTGGCGCTGGCCGCAGCACATCAACGGACAGCCG
ATGGACAGCTATCATCGCTGGATGGAGGTCGTTGTTCCGGTATCATTGATCGGCCTCGCCGCGCTCAGCGTTCCCGTCGG
TTTCGATCAGCGCGGGCGGCCGACGGGCATGCAGATTATCGGCGCCAGCGGCGCCGATGCCGAAATTCTGGCGATCGGCG
AAACCTATCACCGCGCGACCTTGTGGCCGCAAAGGAAACCGCCGATGCTTGCCGCATAA

Upstream 100 bases:

>100_bases
TTGCTGATGATCGCATCTTTATTACCATGGGGTTTACCCATTCTCTGGTTTCGATACAACTCGACGCCTTGCTTGCGCGC
TTCTGACAGGAGTTGACCCG

Downstream 100 bases:

>100_bases
AAGACAAAAGCCGGCTTCCACAAACGAAGCCGGCTTTCTTGCCGATCATTCGGCCAGCCAGTTTTGCCAGCGTTCGCGAA
GCATGTCGCGGTTTTCACCG

Product: amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MDLTELSAARLVNMMGKRQTSPSEVMAAHLDRIERHNGAVNAMVAMRSRDELIAEARLLDDKPVCGPLHGLPWAIKDLLP
TKDIRSTWGSPIHADYIPAEDALAVSRIRKAGAVIMGKTNTPEWGHGSHSFNPVYGVTCNPYDTQLSAGGSSGGTAAALA
AHFMPLGDGSDMMGSLRNPAGFCNIYGYRPSWGLVPNEIGGDLFVHTMATLGPMARDIEDLVLLLDVLAEPYSLSPFGQK
HTGFATNHAPADLRGKRIAWLGNWGGAYPCEAGILERCEKGLAVLSELGAEIVPLEPPFSSQALWEAWITLRSLVIYGSK
RALWEKPETRKLIKPETLWEIENGAGLTAQQIMHASQIRSDWYVSAHRLFAEYDALIMPTAQVWPFPADWRWPQHINGQP
MDSYHRWMEVVVPVSLIGLAALSVPVGFDQRGRPTGMQIIGASGADAEILAIGETYHRATLWPQRKPPMLAA

Sequences:

>Translated_472_residues
MDLTELSAARLVNMMGKRQTSPSEVMAAHLDRIERHNGAVNAMVAMRSRDELIAEARLLDDKPVCGPLHGLPWAIKDLLP
TKDIRSTWGSPIHADYIPAEDALAVSRIRKAGAVIMGKTNTPEWGHGSHSFNPVYGVTCNPYDTQLSAGGSSGGTAAALA
AHFMPLGDGSDMMGSLRNPAGFCNIYGYRPSWGLVPNEIGGDLFVHTMATLGPMARDIEDLVLLLDVLAEPYSLSPFGQK
HTGFATNHAPADLRGKRIAWLGNWGGAYPCEAGILERCEKGLAVLSELGAEIVPLEPPFSSQALWEAWITLRSLVIYGSK
RALWEKPETRKLIKPETLWEIENGAGLTAQQIMHASQIRSDWYVSAHRLFAEYDALIMPTAQVWPFPADWRWPQHINGQP
MDSYHRWMEVVVPVSLIGLAALSVPVGFDQRGRPTGMQIIGASGADAEILAIGETYHRATLWPQRKPPMLAA
>Mature_472_residues
MDLTELSAARLVNMMGKRQTSPSEVMAAHLDRIERHNGAVNAMVAMRSRDELIAEARLLDDKPVCGPLHGLPWAIKDLLP
TKDIRSTWGSPIHADYIPAEDALAVSRIRKAGAVIMGKTNTPEWGHGSHSFNPVYGVTCNPYDTQLSAGGSSGGTAAALA
AHFMPLGDGSDMMGSLRNPAGFCNIYGYRPSWGLVPNEIGGDLFVHTMATLGPMARDIEDLVLLLDVLAEPYSLSPFGQK
HTGFATNHAPADLRGKRIAWLGNWGGAYPCEAGILERCEKGLAVLSELGAEIVPLEPPFSSQALWEAWITLRSLVIYGSK
RALWEKPETRKLIKPETLWEIENGAGLTAQQIMHASQIRSDWYVSAHRLFAEYDALIMPTAQVWPFPADWRWPQHINGQP
MDSYHRWMEVVVPVSLIGLAALSVPVGFDQRGRPTGMQIIGASGADAEILAIGETYHRATLWPQRKPPMLAA

Specific function: Unknown

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=500, Percent_Identity=26.2, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI222831590, Length=254, Percent_Identity=33.8582677165354, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI166795287, Length=212, Percent_Identity=31.6037735849057, Blast_Score=74, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17537465, Length=240, Percent_Identity=31.6666666666667, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17538252, Length=229, Percent_Identity=30.1310043668122, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI71990152, Length=244, Percent_Identity=29.0983606557377, Blast_Score=85, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17543272, Length=173, Percent_Identity=33.5260115606936, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17556264, Length=280, Percent_Identity=28.9285714285714, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17538254, Length=259, Percent_Identity=27.4131274131274, Blast_Score=67, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6319685, Length=430, Percent_Identity=25.3488372093023, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6323950, Length=447, Percent_Identity=25.9507829977629, Blast_Score=81, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6320448, Length=458, Percent_Identity=24.6724890829694, Blast_Score=77, Evalue=6e-15,
Organism=Drosophila melanogaster, GI45550774, Length=491, Percent_Identity=26.4765784114053, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24648435, Length=491, Percent_Identity=26.4765784114053, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24648437, Length=491, Percent_Identity=26.4765784114053, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI21356731, Length=485, Percent_Identity=24.9484536082474, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24644968, Length=493, Percent_Identity=24.7464503042596, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24648441, Length=416, Percent_Identity=26.4423076923077, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24648439, Length=416, Percent_Identity=26.4423076923077, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI161078093, Length=508, Percent_Identity=25.3937007874016, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24648113, Length=440, Percent_Identity=27.2727272727273, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24652985, Length=239, Percent_Identity=33.8912133891213, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI19922090, Length=239, Percent_Identity=33.8912133891213, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24652981, Length=239, Percent_Identity=33.8912133891213, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24652983, Length=239, Percent_Identity=33.8912133891213, Blast_Score=94, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.1.4 [H]

Molecular weight: Translated: 51619; Mature: 51619

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLTELSAARLVNMMGKRQTSPSEVMAAHLDRIERHNGAVNAMVAMRSRDELIAEARLLD
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCC
DKPVCGPLHGLPWAIKDLLPTKDIRSTWGSPIHADYIPAEDALAVSRIRKAGAVIMGKTN
CCCCCCCCCCCCHHHHHHCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCEEEEECCC
TPEWGHGSHSFNPVYGVTCNPYDTQLSAGGSSGGTAAALAAHFMPLGDGSDMMGSLRNPA
CCCCCCCCCCCCCEEEEECCCCCCEECCCCCCCCHHHHHHHHHCCCCCCHHHHHHHCCCC
GFCNIYGYRPSWGLVPNEIGGDLFVHTMATLGPMARDIEDLVLLLDVLAEPYSLSPFGQK
CEEEEEEECCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
HTGFATNHAPADLRGKRIAWLGNWGGAYPCEAGILERCEKGLAVLSELGAEIVPLEPPFS
CCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCC
SQALWEAWITLRSLVIYGSKRALWEKPETRKLIKPETLWEIENGAGLTAQQIMHASQIRS
HHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCHHHEEECCCCCCHHHHHHHHHHHHH
DWYVSAHRLFAEYDALIMPTAQVWPFPADWRWPQHINGQPMDSYHRWMEVVVPVSLIGLA
HHHHHHHHHHHHHCCEEECCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
ALSVPVGFDQRGRPTGMQIIGASGADAEILAIGETYHRATLWPQRKPPMLAA
HHHCCCCCCCCCCCCCEEEEECCCCCCEEEEECCHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MDLTELSAARLVNMMGKRQTSPSEVMAAHLDRIERHNGAVNAMVAMRSRDELIAEARLLD
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCC
DKPVCGPLHGLPWAIKDLLPTKDIRSTWGSPIHADYIPAEDALAVSRIRKAGAVIMGKTN
CCCCCCCCCCCCHHHHHHCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCEEEEECCC
TPEWGHGSHSFNPVYGVTCNPYDTQLSAGGSSGGTAAALAAHFMPLGDGSDMMGSLRNPA
CCCCCCCCCCCCCEEEEECCCCCCEECCCCCCCCHHHHHHHHHCCCCCCHHHHHHHCCCC
GFCNIYGYRPSWGLVPNEIGGDLFVHTMATLGPMARDIEDLVLLLDVLAEPYSLSPFGQK
CEEEEEEECCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
HTGFATNHAPADLRGKRIAWLGNWGGAYPCEAGILERCEKGLAVLSELGAEIVPLEPPFS
CCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCC
SQALWEAWITLRSLVIYGSKRALWEKPETRKLIKPETLWEIENGAGLTAQQIMHASQIRS
HHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCHHHEEECCCCCCHHHHHHHHHHHHH
DWYVSAHRLFAEYDALIMPTAQVWPFPADWRWPQHINGQPMDSYHRWMEVVVPVSLIGLA
HHHHHHHHHHHHHCCEEECCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
ALSVPVGFDQRGRPTGMQIIGASGADAEILAIGETYHRATLWPQRKPPMLAA
HHHCCCCCCCCCCCCCEEEEECCCCCCEEEEECCHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]