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The map label for this gene is insF1 [C]

Identifier: 225686824

GI number: 225686824

Start: 1033955

End: 1034863

Strand: Reverse

Name: insF1 [C]

Synonym: BMEA_B1060

Alternate gene names: 225686824

Gene position: 1034863-1033955 (Counterclockwise)

Preceding gene: 225686825

Following gene: 225686823

Centisome position: 87.29

GC content: 49.28

Gene sequence:

>909_bases
ATGAAGTTTGCGTTCATCGACACGGAGAAGGCCCATATGTCTCTGTCACGGCTGTGCGCTTTCGCAGGCGTCAGCATCAG
CGGTTATTATGCATGGAAGCATCGTTTGCCCAGTCGTCGTCAGCTTGATGACATGATTATTCTGGCCCACATTCGTAATC
AGTTTGCGCTGTCACGTGAAACCTATGGCAGCCCTCGAATGCATGTTGAACTGAACGAAGAAGGTATTCGAGCAGGTCGT
CATCGCACGGCGCGACTGATGCGTGAAAATGGTTTGAAAGCCCGACAGAAAACGCGCTTCAAACGCACGACCGACAGCAA
TCATGGTGAGCCTGTCGCTCCCAATCTTCTGGATCAGGACTTCACTTGTGACAGACCAGATCAGAAATGGGGTGTCGATA
TCAGTTACATATGGACCGCCGAAGGCTGGTTATATCTGGCAATCGTTGTCGATCTTTATTCTCGTCGCATCATTGGCTGG
GAAGCGCGCGATCGAATGAAAAAGGACCTTGCTATTTGTGCTTTGAAGAAGGCCATTGCAATTCGGTACCCAAAGCCGGG
GCTGATCCAACACTCCGACAGGGGAAGCCAATATGCCAGTTATGAGTATCGCAAAATCCTCAAATCGCACAGCATGCTTC
CATCCATGAGCGGCAAAGGAAATTGCTATGACAATGCAATGGTGGAAACCGTCTTCAAAACGATCAAGTCGGAACTGATC
TGGCGCGCTGCATTCCAGACCAGAAATGACGCAATCAAAGCCATTGGCAAATACATTGACGGCTTCTATAATCCCGTGCG
CAGACATTCTACACTGGGCTATAAATCGCCGGTGAAATTCGAGGCAATGAACCGAAACTTAGAGACAGAAGCTCTCCACT
TTTCCGAAGCAAGCCCAGTTTCAATCTGA

Upstream 100 bases:

>100_bases
ACCAGGAGCTTGCCCGGCTTCGTAAAGAAAACGAACTTCTGCGACAGGAACGTGACCTACTAAAAAAAGCGACGGCCTTC
TTCGCTCGGGAGACAAGTCG

Downstream 100 bases:

>100_bases
AGCACGCCATGCGCGAATGTTGATGTTCCGCGATTCTCTCAATTAATCGCTATGCAAACGAATCTCTCTCTCATCGTTTT
CTGTTTGGCAATAGCTAATT

Product: transposase orfB, IS3 family element

Products: NA

Alternate protein names: ISO-S3 31 kDa protein [H]

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MKFAFIDTEKAHMSLSRLCAFAGVSISGYYAWKHRLPSRRQLDDMIILAHIRNQFALSRETYGSPRMHVELNEEGIRAGR
HRTARLMRENGLKARQKTRFKRTTDSNHGEPVAPNLLDQDFTCDRPDQKWGVDISYIWTAEGWLYLAIVVDLYSRRIIGW
EARDRMKKDLAICALKKAIAIRYPKPGLIQHSDRGSQYASYEYRKILKSHSMLPSMSGKGNCYDNAMVETVFKTIKSELI
WRAAFQTRNDAIKAIGKYIDGFYNPVRRHSTLGYKSPVKFEAMNRNLETEALHFSEASPVSI

Sequences:

>Translated_302_residues
MKFAFIDTEKAHMSLSRLCAFAGVSISGYYAWKHRLPSRRQLDDMIILAHIRNQFALSRETYGSPRMHVELNEEGIRAGR
HRTARLMRENGLKARQKTRFKRTTDSNHGEPVAPNLLDQDFTCDRPDQKWGVDISYIWTAEGWLYLAIVVDLYSRRIIGW
EARDRMKKDLAICALKKAIAIRYPKPGLIQHSDRGSQYASYEYRKILKSHSMLPSMSGKGNCYDNAMVETVFKTIKSELI
WRAAFQTRNDAIKAIGKYIDGFYNPVRRHSTLGYKSPVKFEAMNRNLETEALHFSEASPVSI
>Mature_302_residues
MKFAFIDTEKAHMSLSRLCAFAGVSISGYYAWKHRLPSRRQLDDMIILAHIRNQFALSRETYGSPRMHVELNEEGIRAGR
HRTARLMRENGLKARQKTRFKRTTDSNHGEPVAPNLLDQDFTCDRPDQKWGVDISYIWTAEGWLYLAIVVDLYSRRIIGW
EARDRMKKDLAICALKKAIAIRYPKPGLIQHSDRGSQYASYEYRKILKSHSMLPSMSGKGNCYDNAMVETVFKTIKSELI
WRAAFQTRNDAIKAIGKYIDGFYNPVRRHSTLGYKSPVKFEAMNRNLETEALHFSEASPVSI

Specific function: Involved in the transposition of the insertion sequence is3 [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=260, Percent_Identity=32.3076923076923, Blast_Score=135, Evalue=5e-32,
Organism=Escherichia coli, GI1788405, Length=293, Percent_Identity=41.296928327645, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI1787263, Length=293, Percent_Identity=41.296928327645, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI1786753, Length=293, Percent_Identity=41.296928327645, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI1786570, Length=293, Percent_Identity=41.296928327645, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI1786490, Length=293, Percent_Identity=41.296928327645, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI1789981, Length=223, Percent_Identity=33.6322869955157, Blast_Score=122, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 34844; Mature: 34844

Theoretical pI: Translated: 10.20; Mature: 10.20

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFAFIDTEKAHMSLSRLCAFAGVSISGYYAWKHRLPSRRQLDDMIILAHIRNQFALSRE
CEEEEECCHHHHHHHHHHHHHHCCCEEEEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
TYGSPRMHVELNEEGIRAGRHRTARLMRENGLKARQKTRFKRTTDSNHGEPVAPNLLDQD
HCCCCEEEEEECHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCC
FTCDRPDQKWGVDISYIWTAEGWLYLAIVVDLYSRRIIGWEARDRMKKDLAICALKKAIA
CCCCCCCHHHCCEEEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHE
IRYPKPGLIQHSDRGSQYASYEYRKILKSHSMLPSMSGKGNCYDNAMVETVFKTIKSELI
EECCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
WRAAFQTRNDAIKAIGKYIDGFYNPVRRHSTLGYKSPVKFEAMNRNLETEALHFSEASPV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCHHEEECCCCCCC
SI
CC
>Mature Secondary Structure
MKFAFIDTEKAHMSLSRLCAFAGVSISGYYAWKHRLPSRRQLDDMIILAHIRNQFALSRE
CEEEEECCHHHHHHHHHHHHHHCCCEEEEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
TYGSPRMHVELNEEGIRAGRHRTARLMRENGLKARQKTRFKRTTDSNHGEPVAPNLLDQD
HCCCCEEEEEECHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCC
FTCDRPDQKWGVDISYIWTAEGWLYLAIVVDLYSRRIIGWEARDRMKKDLAICALKKAIA
CCCCCCCHHHCCEEEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHE
IRYPKPGLIQHSDRGSQYASYEYRKILKSHSMLPSMSGKGNCYDNAMVETVFKTIKSELI
EECCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
WRAAFQTRNDAIKAIGKYIDGFYNPVRRHSTLGYKSPVKFEAMNRNLETEALHFSEASPV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCHHEEECCCCCCC
SI
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2824781 [H]